Restriction map for
pF1KB9417
(Circular) (Six-base) MAPSORT of: pF1KB9417.seq Check: 4595 from: 1 to: 5768
pF1KB9417 5768 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 2255 2806 2255
Size: 551 5217
Acc65I G'GTAC_C
Cuts at: 1192 1205 1571 2784 1192
Size: 13 366 1213 4176
Fragments arranged by size:
4176 1213 366 13
AclI AA'CG_TT
Cuts at: 5608 5608
Size: 5768
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 1628 1666 2404 2521 3593 4025 4378 5040
Size: 1404 38 738 117 1072 432 353 662
Cuts at: 5040 224
Size: 952
Fragments arranged by size:
1404 1072 952 738 662 432 353 117
38
AflIII A'CryG_T
Cuts at: 2129 3120 4920 5201 2129
Size: 991 1800 281 2696
Fragments arranged by size:
2696 1800 991 281
AhdI GACnn_n'nnGTC
Cuts at: 2677 2677
Size: 5768
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 691 723 691
Size: 32 5736
AlwNI CAG_nnn'CTG
Cuts at: 333 1927 2111 3073 4511 333
Size: 1594 184 962 1438 1590
Fragments arranged by size:
1594 1590 1438 962 184
ApaI G_GGCC'C
Cuts at: 1057 1114 1057
Size: 57 5711
ApaLI G'TGCA_C
Cuts at: 4606 4606
Size: 5768
ApoI r'AATT_y
Cuts at: 1852 2772 5121 5377 1852
Size: 920 2349 256 2243
Fragments arranged by size:
2349 2243 920 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5768
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5768
AvaI C'yCGr_G
Cuts at: 248 301 1175 1990 2788 248
Size: 53 874 815 798 3228
Fragments arranged by size:
3228 874 815 798 53
BamHI G'GATC_C
Cuts at: 2793 2793
Size: 5768
BanI G'GyrC_C
Cuts at: 119 244 262 363 1179 1192 1205 1385 1571
Size: 125 18 101 816 13 13 180 186
Cuts at: 1571 2758 2784 3198 3446 3481 119
Size: 1187 26 414 248 35 2406
Fragments arranged by size:
2406 1187 816 414 248 186 180 125
101 35 26 18 13 13
BanII G_rGCy'C
Cuts at: 299 341 542 611 953 1057 1114 1175 2159
Size: 42 201 69 342 104 57 61 984
Cuts at: 2159 2731 2782 3812 299
Size: 572 51 1030 2255
Fragments arranged by size:
2255 1030 984 572 342 201 104 69
61 57 51 42
BbeI G_GCGC'C
Cuts at: 1183 1389 3202 3450 1183
Size: 206 1813 248 3501
Fragments arranged by size:
3501 1813 248 206
BbsI GAAGACnn'nnnn_
Cuts at: 551 1705 1786 551
Size: 1154 81 4533
Fragments arranged by size:
4533 1154 81
BbvCI CC'TCA_GC
Cuts at: 2654 2654
Size: 5768
BciVI GTATCCnnnnn_n'
Cuts at: 3666 4722 5768 3666
Size: 1056 1046 3666
Fragments arranged by size:
3666 1056 1046
BclI T'GATC_A
Cuts at: 3288 3288
Size: 5768
BglI GCCn_nnn'nGGC
Cuts at: 857 857
Size: 5768
BglII A'GATC_T
Cuts at: 2168 3283 5320 2168
Size: 1115 2037 2616
Fragments arranged by size:
2616 2037 1115
BlpI GC'TnA_GC
Cuts at: 2711 2880 2711
Size: 169 5599
Bme1580I G_kGCm'C
Cuts at: 124 196 249 265 368 977 1057 1106 1114
Size: 72 53 16 103 609 80 49 8
Cuts at: 1114 3393 3486 4610 124
Size: 2279 93 1124 1282
Fragments arranged by size:
2279 1282 1124 609 103 93 80 72
53 49 16 8
BmrI ACTGGGnnnn_n'
Cuts at: 1360 1384 2980 3061 3396 5540 1360
Size: 24 1596 81 335 2144 1588
Fragments arranged by size:
2144 1596 1588 335 81 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4135 4135
Size: 5768
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 355 532 553 613 641 830 850 1300
Size: 90 177 21 60 28 189 20 450
Cuts at: 1300 1377 1396 1837 2603 265
Size: 77 19 441 766 3430
Fragments arranged by size:
3430 766 450 441 189 177 90 77
60 28 21 20 19
Bpu10I CC'TnA_GC
Cuts at: 580 593 853 1261 2654 580
Size: 13 260 408 1393 3694
Fragments arranged by size:
3694 1393 408 260 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1750 2944 3823 4537 4835 1750
Size: 1194 879 714 298 2683
Fragments arranged by size:
2683 1194 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 813 1507 1952 5472 813
Size: 694 445 3520 1109
Fragments arranged by size:
3520 1109 694 445
BsaAI yAC'GTr
Cuts at: 3121 3121
Size: 5768
BsaBI GATnn'nnATC
Cuts at: 6 916 1795 3282 5319 6
Size: 910 879 1487 2037 455
Fragments arranged by size:
2037 1487 910 879 455
BsaHI Gr'CG_yC
Cuts at: 1090 1180 1386 3199 3447 4149 5645 1090
Size: 90 206 1813 248 702 1496 1213
Fragments arranged by size:
1813 1496 1213 702 248 206 90
BsaWI w'CCGG_w
Cuts at: 2957 3478 4156 4567 4714 4988 2957
Size: 521 678 411 147 274 3737
Fragments arranged by size:
3737 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 611 641 793 823 1439 1469 611
Size: 30 152 30 616 30 4910
Fragments arranged by size:
4910 616 152 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 550 961 1966 2609 550
Size: 411 1005 643 3709
Fragments arranged by size:
3709 1005 643 411
BseYI C'CCAG_C
Cuts at: 170 491 671 782 1342 1920 2435 2612 2733
Size: 321 180 111 560 578 515 177 121
Cuts at: 2733 3193 3951 4616 5024 170
Size: 460 758 665 408 914
Fragments arranged by size:
914 758 665 578 560 515 460 408
321 180 177 121 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 864 1785 864
Size: 921 4847
BsiEI CG_ry'CG
Cuts at: 104 3310 3356 4586 104
Size: 3206 46 1230 1286
Fragments arranged by size:
3206 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 341 779 2782 3560 3750 4610 341
Size: 438 2003 778 190 860 1499
Fragments arranged by size:
2003 1499 860 778 438 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1081 1081
Size: 5768
Bsp1286I G_dGCh'C
Cuts at: 124 196 249 265 299 341 368 542 611
Size: 72 53 16 34 42 27 174 69
Cuts at: 611 779 953 977 1057 1106 1114 1175 2159
Size: 168 174 24 80 49 8 61 984
Cuts at: 2159 2731 2782 3393 3486 3560 3750 3812 4610
Size: 572 51 611 93 74 190 62 798
Cuts at: 4610 124
Size: 1282
Fragments arranged by size:
1282 984 798 611 572 190 174 174
168 93 80 74 72 69 62 61
53 51 49 42 34 27 24 16
8
BspHI T'CATG_A
Cuts at: 1871 1946 4200 1871
Size: 75 2254 3439
Fragments arranged by size:
3439 2254 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2018 2818 3032 3334 3715 2018
Size: 800 214 302 381 4071
Fragments arranged by size:
4071 800 381 302 214
BsrBI CCG'CTC
Cuts at: 50 857 2710 4060 4114 5598 5763 50
Size: 807 1853 1350 54 1484 165 55
Fragments arranged by size:
1853 1484 1350 807 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3680 3680
Size: 5768
BsrFI r'CCGG_y
Cuts at: 1208 1499 1676 2456 3766 3947 1208
Size: 291 177 780 1310 181 3029
Fragments arranged by size:
3029 1310 780 291 181 177
BssSI C'ACGA_G
Cuts at: 1419 1803 4039 4747 1419
Size: 384 2236 708 2440
Fragments arranged by size:
2440 2236 708 384
BstAPI GCAn_nnn'nTGC
Cuts at: 894 3145 894
Size: 2251 3517
BstBI TT'CG_AA
Cuts at: 4129 4129
Size: 5768
BstEII G'GTnAC_C
Cuts at: 744 1272 744
Size: 528 5240
BstXI CCAn_nnnn'nTGG
Cuts at: 828 2058 2222 2668 828
Size: 1230 164 446 3928
Fragments arranged by size:
3928 1230 446 164
BstYI r'GATC_y
Cuts at: 1 329 2168 2793 3260 3283 3618 3864 4170
Size: 328 1839 625 467 23 335 246 306
Cuts at: 4170 4182 4268 5320 1
Size: 12 86 1052 449
Fragments arranged by size:
1839 1052 625 467 449 335 328 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2624 2624
Size: 5768
BtgI C'CryG_G
Cuts at: 107 611 650 821 107
Size: 504 39 171 5054
Fragments arranged by size:
5054 504 171 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2031 2285 3718 3899 4062 2031
Size: 254 1433 181 163 3737
Fragments arranged by size:
3737 1433 254 181 163
BtsI GCAGTG_nn'
Cuts at: 876 1334 2645 3011 876
Size: 458 1311 366 3633
Fragments arranged by size:
3633 1311 458 366
DraI TTT'AAA
Cuts at: 2767 2767
Size: 5768
DraIII CAC_nnn'GTG
Cuts at: 1686 1686
Size: 5768
DrdI GACnn_nn'nnGTC
Cuts at: 486 3474 4818 486
Size: 2988 1344 1436
Fragments arranged by size:
2988 1436 1344
EaeI y'GGCC_r
Cuts at: 557 647 1457 2102 2458 3353 3527 3918 3945
Size: 90 810 645 356 895 174 391 27
Cuts at: 3945 5290 557
Size: 1345 1035
Fragments arranged by size:
1345 1035 895 810 645 391 356 174
90 27
EagI C'GGCC_G
Cuts at: 3353 3353
Size: 5768
EarI CTCTTCn'nnn_
Cuts at: 1492 1634 3791 4001 1492
Size: 142 2157 210 3259
Fragments arranged by size:
3259 2157 210 142
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2161 3976 4718 4864 5578 2161
Size: 1815 742 146 714 2351
Fragments arranged by size:
2351 1815 742 714 146
EcoICRI GAG'CTC
Cuts at: 339 2780 339
Size: 2441 3327
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 265 355 532 553 613 641 830 850
Size: 41 90 177 21 60 28 189 20
Cuts at: 850 1300 1377 1396 1628 1666 1837 2404 2521
Size: 450 77 19 232 38 171 567 117
Cuts at: 2521 2603 3593 4025 4378 5040 224
Size: 82 990 432 353 662 952
Fragments arranged by size:
990 952 662 567 450 432 353 232
189 177 171 117 90 82 77 60
41 38 28 21 20 19
EcoNI CCTnn'n_nnAGG
Cuts at: 373 373
Size: 5768
EcoO109I rG'GnC_Cy
Cuts at: 138 598 1053 1054 1111 2094 2907 138
Size: 460 455 1 57 983 813 2999
Fragments arranged by size:
2999 983 813 460 455 57 1
EcoRI G'AATT_C
Cuts at: 2772 2772
Size: 5768
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3787 3787
Size: 5768
FspI TGC'GCA
Cuts at: 1582 3549 1582
Size: 1967 3801
HaeII r_GCGC'y
Cuts at: 163 1183 1389 3202 3450 4680 5451 163
Size: 1020 206 1813 248 1230 771 480
Fragments arranged by size:
1813 1230 1020 771 480 248 206
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2116 2148 2263 2295 2663 2695 2713 2745 3610
Size: 32 115 32 368 32 18 32 865
Cuts at: 3610 3640 3642 3672 2116
Size: 30 2 30 4212
Fragments arranged by size:
4212 865 368 115 32 32 32 32
30 30 18 2
HincII GTy'rAC
Cuts at: 2547 2807 2547
Size: 260 5508
KasI G'GCGC_C
Cuts at: 1179 1385 3198 3446 1179
Size: 206 1813 248 3501
Fragments arranged by size:
3501 1813 248 206
KpnI G_GTAC'C
Cuts at: 1196 1209 1575 2788 1196
Size: 13 366 1213 4176
Fragments arranged by size:
4176 1213 366 13
MluI A'CGCG_T
Cuts at: 5201 5201
Size: 5768
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 461 1579 4527 4711 461
Size: 1118 2948 184 1518
Fragments arranged by size:
2948 1518 1118 184
MscI TGG'CCA
Cuts at: 649 3529 649
Size: 2880 2888
MslI CAynn'nnrTG
Cuts at: 772 1030 2020 2134 3757 3884 772
Size: 258 990 114 1623 127 2656
Fragments arranged by size:
2656 1623 990 258 127 114
MspA1I CmG'CkG
Cuts at: 144 1462 2879 3193 3553 4337 4582 144
Size: 1318 1417 314 360 784 245 1330
Fragments arranged by size:
1417 1330 1318 784 360 314 245
NaeI GCC'GGC
Cuts at: 3949 3949
Size: 5768
NarI GG'CG_CC
Cuts at: 1180 1386 3199 3447 1180
Size: 206 1813 248 3501
Fragments arranged by size:
3501 1813 248 206
NcoI C'CATG_G
Cuts at: 107 107
Size: 5768
NgoMIV G'CCGG_C
Cuts at: 3947 3947
Size: 5768
NspI r_CATG'y
Cuts at: 688 1040 2118 2133 2821 3321 4924 688
Size: 352 1078 15 688 500 1603 1532
Fragments arranged by size:
1603 1532 1078 688 500 352 15
PasI CC'CwG_GG
Cuts at: 188 229 369 1107 1692 2743 188
Size: 41 140 738 585 1051 3213
Fragments arranged by size:
3213 1051 738 585 140 41
PciI A'CATG_T
Cuts at: 2129 4920 2129
Size: 2791 2977
PflMI CCAn_nnn'nTGG
Cuts at: 113 459 113
Size: 346 5422
PfoI T'CCnGG_A
Cuts at: 332 530 629 1063 332
Size: 198 99 434 5037
Fragments arranged by size:
5037 434 198 99
PmeI GTTT'AAAC
Cuts at: 2767 2767
Size: 5768
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4214 4246 4214
Size: 32 5736
PpuMI rG'GwC_Cy
Cuts at: 138 598 2094 138
Size: 460 1496 3812
Fragments arranged by size:
3812 1496 460
PspOMI G'GGCC_C
Cuts at: 1053 1110 1053
Size: 57 5711
PstI C_TGCA'G
Cuts at: 431 890 1190 1289 2592 2815 3500 431
Size: 459 300 99 1303 223 685 2699
Fragments arranged by size:
2699 1303 685 459 300 223 99
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5768
PvuII CAG'CTG
Cuts at: 3193 3553 3193
Size: 360 5408
RsrII CG'GwC_CG
Cuts at: 3963 3963
Size: 5768
SacI G_AGCT'C
Cuts at: 341 2782 341
Size: 2441 3327
SalI G'TCGA_C
Cuts at: 2805 2805
Size: 5768
SanDI GG'GwC_CC
Cuts at: 138 598 138
Size: 460 5308
SapI GCTCTTCn'nnn_
Cuts at: 1634 3791 4001 1634
Size: 2157 210 3401
Fragments arranged by size:
3401 2157 210
SbfI CC_TGCA'GG
Cuts at: 890 1190 2815 890
Size: 300 1625 3843
Fragments arranged by size:
3843 1625 300
ScaI AGT'ACT
Cuts at: 5285 5285
Size: 5768
SexAI A'CCwGG_T
Cuts at: 803 803
Size: 5768
SfcI C'TryA_G
Cuts at: 33 427 886 1186 1285 1590 2588 2634 2811
Size: 394 459 300 99 305 998 46 177
Cuts at: 2811 3496 4464 4655 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 998 968 685 459 394 305 300
191 177 99 46
SfoI GGC'GCC
Cuts at: 1181 1387 3200 3448 1181
Size: 206 1813 248 3501
Fragments arranged by size:
3501 1813 248 206
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5768
SgrAI Cr'CCGG_yG
Cuts at: 1499 1499
Size: 5768
SmaI CCC'GGG
Cuts at: 250 1177 2790 250
Size: 927 1613 3228
Fragments arranged by size:
3228 1613 927
SmlI C'TyrA_G
Cuts at: 1765 2923 3838 4552 4814 1765
Size: 1158 915 714 262 2719
Fragments arranged by size:
2719 1158 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5768
SphI G_CATG'C
Cuts at: 1040 2118 2821 3321 1040
Size: 1078 703 500 3487
Fragments arranged by size:
3487 1078 703 500
StuI AGG'CCT
Cuts at: 851 1153 1381 2506 851
Size: 302 228 1125 4113
Fragments arranged by size:
4113 1125 302 228
StyI C'CwwG_G
Cuts at: 107 737 2902 3256 107
Size: 630 2165 354 2619
Fragments arranged by size:
2619 2165 630 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 361 367 3351 3484 3943 4152 361
Size: 6 2984 133 459 209 1977
Fragments arranged by size:
2984 1977 459 209 133 6
TatI w'GTAC_w
Cuts at: 1437 5283 1437
Size: 3846 1922
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 793 825 1439 1471 5285 5317 793
Size: 32 614 32 3814 32 1244
Fragments arranged by size:
3814 1244 614 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2251 3565 2251
Size: 1314 4454
XbaI T'CTAG_A
Cuts at: 2799 2799
Size: 5768
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1767 3263 1767
Size: 1496 4272
XmaI C'CCGG_G
Cuts at: 248 1175 2788 248
Size: 927 1613 3228
Fragments arranged by size:
3228 1613 927
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BspEI BsrGI BssHII BstZ17I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspXI PsrI SacII
SfiI SnaBI SrfI SspI SwaI XhoI XmnI ZraI