Restriction map for
pF1KB9409
(Circular) (Six-base) MAPSORT of: pF1KB9409.seq Check: 2427 from: 1 to: 3980
pF1KB9409 3980 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1018 1018
Size: 3980
Acc65I G'GTAC_C
Cuts at: 996 996
Size: 3980
AclI AA'CG_TT
Cuts at: 3820 3820
Size: 3980
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1805 2237 2590 3252 1805
Size: 432 353 662 2533
Fragments arranged by size:
2533 662 432 353
AfeI AGC'GCT
Cuts at: 214 214
Size: 3980
AflIII A'CryG_T
Cuts at: 1332 3132 3413 1332
Size: 1800 281 1899
Fragments arranged by size:
1899 1800 281
AleI CACnn'nnGTG
Cuts at: 823 823
Size: 3980
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 166 166
Size: 3980
AlwNI CAG_nnn'CTG
Cuts at: 195 513 1285 2723 195
Size: 318 772 1438 1452
Fragments arranged by size:
1452 1438 772 318
ApaLI G'TGCA_C
Cuts at: 2818 2818
Size: 3980
ApoI r'AATT_y
Cuts at: 984 3333 3589 984
Size: 2349 256 1375
Fragments arranged by size:
2349 1375 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3980
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3980
AvaI C'yCGr_G
Cuts at: 1000 1000
Size: 3980
BamHI G'GATC_C
Cuts at: 910 1005 910
Size: 95 3885
BanI G'GyrC_C
Cuts at: 996 1410 1658 1693 996
Size: 414 248 35 3283
Fragments arranged by size:
3283 414 248 35
BanII G_rGCy'C
Cuts at: 749 772 994 2024 749
Size: 23 222 1030 2705
Fragments arranged by size:
2705 1030 222 23
BbeI G_GCGC'C
Cuts at: 1414 1662 1414
Size: 248 3732
BbsI GAAGACnn'nnnn_
Cuts at: 327 327
Size: 3980
BciVI GTATCCnnnnn_n'
Cuts at: 1878 2934 3980 1878
Size: 1056 1046 1878
Fragments arranged by size:
1878 1056 1046
BclI T'GATC_A
Cuts at: 1500 1500
Size: 3980
BglII A'GATC_T
Cuts at: 1495 3532 1495
Size: 2037 1943
BlpI GC'TnA_GC
Cuts at: 1092 1092
Size: 3980
Bme1580I G_kGCm'C
Cuts at: 1605 1698 2822 1605
Size: 93 1124 2763
Fragments arranged by size:
2763 1124 93
BmgBI CAC'GTC
Cuts at: 825 957 825
Size: 132 3848
BmrI ACTGGGnnnn_n'
Cuts at: 1192 1273 1608 3752 1192
Size: 81 335 2144 1420
Fragments arranged by size:
2144 1420 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2347 2347
Size: 3980
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 538 733 319
Size: 219 195 3566
Fragments arranged by size:
3566 219 195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1156 2035 2749 3047 1156
Size: 879 714 298 2089
Fragments arranged by size:
2089 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 442 3684 442
Size: 3242 738
BsaAI yAC'GTr
Cuts at: 1333 1333
Size: 3980
BsaBI GATnn'nnATC
Cuts at: 6 1494 3531 6
Size: 1488 2037 455
Fragments arranged by size:
2037 1488 455
BsaHI Gr'CG_yC
Cuts at: 1411 1659 2361 3857 1411
Size: 248 702 1496 1534
Fragments arranged by size:
1534 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1169 1690 2368 2779 2926 3200 1169
Size: 521 678 411 147 274 1949
Fragments arranged by size:
1949 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 103 133 460 490 613 643 103
Size: 30 327 30 123 30 3440
Fragments arranged by size:
3440 327 123 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 223 563 859 223
Size: 340 296 3344
Fragments arranged by size:
3344 340 296
BseYI C'CCAG_C
Cuts at: 373 537 611 1405 2163 2828 3236 373
Size: 164 74 794 758 665 408 1117
Fragments arranged by size:
1117 794 758 665 408 164 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 268
Size: 3980
BsiEI CG_ry'CG
Cuts at: 104 180 1522 1568 2798 104
Size: 76 1342 46 1230 1286
Fragments arranged by size:
1342 1286 1230 76 46
BsiHKAI G_wGCw'C
Cuts at: 232 593 772 869 994 1772 1962 2822 232
Size: 361 179 97 125 778 190 860 1390
Fragments arranged by size:
1390 860 778 361 190 179 125 97
BsmBI CGTCTCn'nnnn_
Cuts at: 167 167
Size: 3980
Bsp1286I G_dGCh'C
Cuts at: 232 593 749 772 869 994 1605 1698 1772
Size: 361 156 23 97 125 611 93 74
Cuts at: 1772 1962 2024 2822 232
Size: 190 62 798 1390
Fragments arranged by size:
1390 798 611 361 190 156 125 97
93 74 62 23
BspHI T'CATG_A
Cuts at: 576 2412 576
Size: 1836 2144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 173 203 1030 1244 1546 1927 173
Size: 30 827 214 302 381 2226
Fragments arranged by size:
2226 827 381 302 214 30
BsrBI CCG'CTC
Cuts at: 50 2272 2326 3810 3975 50
Size: 2222 54 1484 165 55
Fragments arranged by size:
2222 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 659 1892 659
Size: 1233 2747
BsrFI r'CCGG_y
Cuts at: 1978 2159 1978
Size: 181 3799
BssSI C'ACGA_G
Cuts at: 124 2251 2959 124
Size: 2127 708 1145
Fragments arranged by size:
2127 1145 708
BstAPI GCAn_nnn'nTGC
Cuts at: 195 597 1357 195
Size: 402 760 2818
Fragments arranged by size:
2818 760 402
BstBI TT'CG_AA
Cuts at: 2341 2341
Size: 3980
BstYI r'GATC_y
Cuts at: 1 910 1005 1472 1495 1830 2076 2382 2394
Size: 909 95 467 23 335 246 306 12
Cuts at: 2394 2480 3532 1
Size: 86 1052 449
Fragments arranged by size:
1052 909 467 449 335 306 246 95
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 506 506
Size: 3980
BtgI C'CryG_G
Cuts at: 107 464 107
Size: 357 3623
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1930 2111 2274 1930
Size: 181 163 3636
Fragments arranged by size:
3636 181 163
BtsI GCAGTG_nn'
Cuts at: 232 1223 232
Size: 991 2989
DraI TTT'AAA
Cuts at: 979 979
Size: 3980
DraIII CAC_nnn'GTG
Cuts at: 129 129
Size: 3980
DrdI GACnn_nn'nnGTC
Cuts at: 181 390 1686 3030 181
Size: 209 1296 1344 1131
Fragments arranged by size:
1344 1296 1131 209
EaeI y'GGCC_r
Cuts at: 284 344 1565 1739 2130 2157 3502 284
Size: 60 1221 174 391 27 1345 762
Fragments arranged by size:
1345 1221 762 391 174 60 27
EagI C'GGCC_G
Cuts at: 1565 1565
Size: 3980
EarI CTCTTCn'nnn_
Cuts at: 323 2003 2213 323
Size: 1680 210 2090
Fragments arranged by size:
2090 1680 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2188 2930 3076 3790 2188
Size: 742 146 714 2378
Fragments arranged by size:
2378 742 714 146
EcoICRI GAG'CTC
Cuts at: 770 992 770
Size: 222 3758
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 538 733 1805 2237 2590 3252 319
Size: 219 195 1072 432 353 662 1047
Fragments arranged by size:
1072 1047 662 432 353 219 195
EcoO109I rG'GnC_Cy
Cuts at: 378 1119 378
Size: 741 3239
EcoRI G'AATT_C
Cuts at: 984 984
Size: 3980
EcoRV GAT'ATC
Cuts at: 966 966
Size: 3980
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1999 1999
Size: 3980
FspI TGC'GCA
Cuts at: 274 601 1761 274
Size: 327 1160 2493
Fragments arranged by size:
2493 1160 327
HaeII r_GCGC'y
Cuts at: 216 1414 1662 2892 3663 216
Size: 1198 248 1230 771 533
Fragments arranged by size:
1230 1198 771 533 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 613 645 722 754 1822 1852 1854 1884 613
Size: 32 77 32 1068 30 2 30 2709
Fragments arranged by size:
2709 1068 77 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1019 1019
Size: 3980
HindIII A'AGCT_T
Cuts at: 839 839
Size: 3980
KasI G'GCGC_C
Cuts at: 1410 1658 1410
Size: 248 3732
KpnI G_GTAC'C
Cuts at: 1000 1000
Size: 3980
MluI A'CGCG_T
Cuts at: 3413 3413
Size: 3980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 285 899 2739 2923 285
Size: 614 1840 184 1342
Fragments arranged by size:
1840 1342 614 184
MscI TGG'CCA
Cuts at: 286 346 1741 286
Size: 60 1395 2525
Fragments arranged by size:
2525 1395 60
MslI CAynn'nnrTG
Cuts at: 292 823 1969 2096 292
Size: 531 1146 127 2176
Fragments arranged by size:
2176 1146 531 127
MspA1I CmG'CkG
Cuts at: 459 466 565 618 1091 1405 1765 2549 2794
Size: 7 99 53 473 314 360 784 245
Cuts at: 2794 459
Size: 1645
Fragments arranged by size:
1645 784 473 360 314 245 99 53
7
NaeI GCC'GGC
Cuts at: 2161 2161
Size: 3980
NarI GG'CG_CC
Cuts at: 1411 1659 1411
Size: 248 3732
NcoI C'CATG_G
Cuts at: 107 107
Size: 3980
NgoMIV G'CCGG_C
Cuts at: 2159 2159
Size: 3980
NspI r_CATG'y
Cuts at: 273 426 649 1033 1533 3136 273
Size: 153 223 384 500 1603 1117
Fragments arranged by size:
1603 1117 500 384 223 153
PciI A'CATG_T
Cuts at: 3132 3132
Size: 3980
PflMI CCAn_nnn'nTGG
Cuts at: 129 129
Size: 3980
PmeI GTTT'AAAC
Cuts at: 979 979
Size: 3980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2426 2458 2426
Size: 32 3948
PpuMI rG'GwC_Cy
Cuts at: 378 378
Size: 3980
PstI C_TGCA'G
Cuts at: 191 884 1027 1712 191
Size: 693 143 685 2459
Fragments arranged by size:
2459 693 685 143
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3980
PvuII CAG'CTG
Cuts at: 565 1405 1765 565
Size: 840 360 2780
Fragments arranged by size:
2780 840 360
RsrII CG'GwC_CG
Cuts at: 138 2175 138
Size: 2037 1943
SacI G_AGCT'C
Cuts at: 772 994 772
Size: 222 3758
SacII CC_GC'GG
Cuts at: 467 467
Size: 3980
SalI G'TCGA_C
Cuts at: 1017 1017
Size: 3980
SanDI GG'GwC_CC
Cuts at: 378 378
Size: 3980
SapI GCTCTTCn'nnn_
Cuts at: 2003 2213 2003
Size: 210 3770
SbfI CC_TGCA'GG
Cuts at: 1027 1027
Size: 3980
ScaI AGT'ACT
Cuts at: 3497 3497
Size: 3980
SexAI A'CCwGG_T
Cuts at: 971 971
Size: 3980
SfcI C'TryA_G
Cuts at: 33 187 880 950 1023 1708 2676 2867 33
Size: 154 693 70 73 685 968 191 1146
Fragments arranged by size:
1146 968 693 685 191 154 73 70
SfoI GGC'GCC
Cuts at: 1412 1660 1412
Size: 248 3732
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3980
SmaI CCC'GGG
Cuts at: 1002 1002
Size: 3980
SmlI C'TyrA_G
Cuts at: 1135 2050 2764 3026 1135
Size: 915 714 262 2089
Fragments arranged by size:
2089 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3980
SphI G_CATG'C
Cuts at: 649 1033 1533 649
Size: 384 500 3096
Fragments arranged by size:
3096 500 384
StyI C'CwwG_G
Cuts at: 107 1114 1468 107
Size: 1007 354 2619
Fragments arranged by size:
2619 1007 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 400 1563 1696 2155 2364 400
Size: 1163 133 459 209 2016
Fragments arranged by size:
2016 1163 459 209 133
TatI w'GTAC_w
Cuts at: 3495 3495
Size: 3980
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 103 135 304 336 460 492 3497 3529 103
Size: 32 169 32 124 32 3005 32 554
Fragments arranged by size:
3005 554 169 124 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 136 181 1777 136
Size: 45 1596 2339
Fragments arranged by size:
2339 1596 45
XbaI T'CTAG_A
Cuts at: 1011 1011
Size: 3980
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 294 354 822 1475 294
Size: 60 468 653 2799
Fragments arranged by size:
2799 653 468 60
XmaI C'CCGG_G
Cuts at: 1000 1000
Size: 3980
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I
BsiWI BsmI BspEI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI EcoNI FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI