Restriction map for
pF1KB9404
(Circular) (Six-base) MAPSORT of: pF1KB9404.seq Check: 4599 from: 1 to: 5495
pF1KB9404 5495 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1083 2533 1083
Size: 1450 4045
Acc65I G'GTAC_C
Cuts at: 2511 2511
Size: 5495
AclI AA'CG_TT
Cuts at: 5335 5335
Size: 5495
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 1796 3320 3752 4105 4767 391
Size: 1405 1524 432 353 662 1119
Fragments arranged by size:
1524 1405 1119 662 432 353
AflIII A'CryG_T
Cuts at: 1986 2487 2847 4647 4928 1986
Size: 501 360 1800 281 2553
Fragments arranged by size:
2553 1800 501 360 281
AhdI GACnn_n'nnGTC
Cuts at: 2233 2233
Size: 5495
AlwNI CAG_nnn'CTG
Cuts at: 2800 4238 2800
Size: 1438 4057
ApaLI G'TGCA_C
Cuts at: 4333 4333
Size: 5495
ApoI r'AATT_y
Cuts at: 510 2499 4848 5104 510
Size: 1989 2349 256 901
Fragments arranged by size:
2349 1989 901 256
AseI AT'TA_AT
Cuts at: 20 908 1640 2455 20
Size: 888 732 815 3060
Fragments arranged by size:
3060 888 815 732
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5495
AvaI C'yCGr_G
Cuts at: 1004 2515 1004
Size: 1511 3984
BamHI G'GATC_C
Cuts at: 1767 1915 2520 1767
Size: 148 605 4742
Fragments arranged by size:
4742 605 148
BanI G'GyrC_C
Cuts at: 2511 2925 3173 3208 2511
Size: 414 248 35 4798
Fragments arranged by size:
4798 414 248 35
BanII G_rGCy'C
Cuts at: 895 2139 2509 3539 895
Size: 1244 370 1030 2851
Fragments arranged by size:
2851 1244 1030 370
BbeI G_GCGC'C
Cuts at: 2929 3177 2929
Size: 248 5247
BbsI GAAGACnn'nnnn_
Cuts at: 1298 1406 1298
Size: 108 5387
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 372 406 1861 1895 372
Size: 34 1455 34 3972
Fragments arranged by size:
3972 1455 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3393 4449 5495 3393
Size: 1056 1046 3393
Fragments arranged by size:
3393 1056 1046
BclI T'GATC_A
Cuts at: 1074 3015 1074
Size: 1941 3554
BglII A'GATC_T
Cuts at: 3010 5047 3010
Size: 2037 3458
BlpI GC'TnA_GC
Cuts at: 2607 2607
Size: 5495
Bme1580I G_kGCm'C
Cuts at: 3120 3213 4337 3120
Size: 93 1124 4278
Fragments arranged by size:
4278 1124 93
BmgBI CAC'GTC
Cuts at: 2488 2488
Size: 5495
BmrI ACTGGGnnnn_n'
Cuts at: 300 515 858 2707 2788 3123 5267 300
Size: 215 343 1849 81 335 2144 528
Fragments arranged by size:
2144 1849 528 343 335 215 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3862 3862
Size: 5495
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2153 2153
Size: 5495
Bpu10I CC'TnA_GC
Cuts at: 440 550 939 440
Size: 110 389 4996
Fragments arranged by size:
4996 389 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1045 1445 1507 2671 3550 4264 4562 1045
Size: 400 62 1164 879 714 298 1978
Fragments arranged by size:
1978 1164 879 714 400 298 62
BsaI GGTCTCn'nnnn_
Cuts at: 5199 5199
Size: 5495
BsaAI yAC'GTr
Cuts at: 2848 2848
Size: 5495
BsaBI GATnn'nnATC
Cuts at: 6 503 1337 3009 5046 6
Size: 497 834 1672 2037 455
Fragments arranged by size:
2037 1672 834 497 455
BsaHI Gr'CG_yC
Cuts at: 1860 2926 3174 3876 5372 1860
Size: 1066 248 702 1496 1983
Fragments arranged by size:
1983 1496 1066 702 248
BsaWI w'CCGG_w
Cuts at: 1719 2684 3205 3883 4294 4441 4715 1719
Size: 965 521 678 411 147 274 2499
Fragments arranged by size:
2499 965 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 786 816 786
Size: 30 5465
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 983 1305 1726 983
Size: 322 421 4752
Fragments arranged by size:
4752 421 322
BseYI C'CCAG_C
Cuts at: 1151 2920 3678 4343 4751 1151
Size: 1769 758 665 408 1895
Fragments arranged by size:
1895 1769 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1121 1121
Size: 5495
BsiEI CG_ry'CG
Cuts at: 104 3037 3083 4313 104
Size: 2933 46 1230 1286
Fragments arranged by size:
2933 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2509 3287 3477 4337 2509
Size: 778 190 860 3667
Fragments arranged by size:
3667 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 895 2139 2509 3120 3213 3287 3477 3539 4337
Size: 1244 370 611 93 74 190 62 798
Cuts at: 4337 895
Size: 2053
Fragments arranged by size:
2053 1244 798 611 370 190 93 74
62
BspHI T'CATG_A
Cuts at: 2076 3927 2076
Size: 1851 3644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 345 2545 2759 3061 3442 345
Size: 2200 214 302 381 2398
Fragments arranged by size:
2398 2200 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3787 3841 5325 5490 50
Size: 3737 54 1484 165 55
Fragments arranged by size:
3737 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3407 3407
Size: 5495
BsrFI r'CCGG_y
Cuts at: 3493 3674 3493
Size: 181 5314
BssSI C'ACGA_G
Cuts at: 3766 4474 3766
Size: 708 4787
BstAPI GCAn_nnn'nTGC
Cuts at: 399 2872 399
Size: 2473 3022
BstBI TT'CG_AA
Cuts at: 1398 3856 1398
Size: 2458 3037
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1245 114
Size: 1131 4364
BstYI r'GATC_y
Cuts at: 1 1108 1767 1915 2520 2987 3010 3345 3591
Size: 1107 659 148 605 467 23 335 246
Cuts at: 3591 3897 3909 3995 5047 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1107 1052 659 605 467 449 335 306
246 148 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3445 3626 3789 3445
Size: 181 163 5151
Fragments arranged by size:
5151 181 163
BtsI GCAGTG_nn'
Cuts at: 2051 2738 2051
Size: 687 4808
DraI TTT'AAA
Cuts at: 1653 1791 2494 1653
Size: 138 703 4654
Fragments arranged by size:
4654 703 138
DrdI GACnn_nn'nnGTC
Cuts at: 3201 4545 3201
Size: 1344 4151
EaeI y'GGCC_r
Cuts at: 3080 3254 3645 3672 5017 3080
Size: 174 391 27 1345 3558
Fragments arranged by size:
3558 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3080 3080
Size: 5495
EarI CTCTTCn'nnn_
Cuts at: 878 1703 2310 3518 3728 878
Size: 825 607 1208 210 2645
Fragments arranged by size:
2645 1208 825 607 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3703 4445 4591 5305 3703
Size: 742 146 714 3893
Fragments arranged by size:
3893 742 714 146
EcoICRI GAG'CTC
Cuts at: 2507 2507
Size: 5495
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 1796 2153 3320 3752 4105 4767 391
Size: 1405 357 1167 432 353 662 1119
Fragments arranged by size:
1405 1167 1119 662 432 357 353
EcoO109I rG'GnC_Cy
Cuts at: 2250 2634 2250
Size: 384 5111
EcoRI G'AATT_C
Cuts at: 2499 2499
Size: 5495
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1854 3514 1854
Size: 1660 3835
FspI TGC'GCA
Cuts at: 3276 3276
Size: 5495
HaeII r_GCGC'y
Cuts at: 2929 3177 4407 5178 2929
Size: 248 1230 771 3246
Fragments arranged by size:
3246 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 265 297 387 419 733 765 1249 1281 1907
Size: 32 90 32 314 32 484 32 626
Cuts at: 1907 1939 2219 2251 3337 3367 3369 3399 265
Size: 32 280 32 1086 30 2 30 2361
Fragments arranged by size:
2361 1086 626 484 314 280 90 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 546 2534 546
Size: 1988 3507
HindIII A'AGCT_T
Cuts at: 1777 1777
Size: 5495
KasI G'GCGC_C
Cuts at: 2925 3173 2925
Size: 248 5247
KpnI G_GTAC'C
Cuts at: 2515 2515
Size: 5495
MfeI C'AATT_G
Cuts at: 1535 2366 1535
Size: 831 4664
MluI A'CGCG_T
Cuts at: 4928 4928
Size: 5495
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 979 1294 2328 4254 4438 979
Size: 315 1034 1926 184 2036
Fragments arranged by size:
2036 1926 1034 315 184
MscI TGG'CCA
Cuts at: 3256 3256
Size: 5495
MslI CAynn'nnrTG
Cuts at: 112 232 1070 2192 3484 3611 112
Size: 120 838 1122 1292 127 1996
Fragments arranged by size:
1996 1292 1122 838 127 120
MspA1I CmG'CkG
Cuts at: 2606 2920 3280 4064 4309 2606
Size: 314 360 784 245 3792
Fragments arranged by size:
3792 784 360 314 245
NaeI GCC'GGC
Cuts at: 3676 3676
Size: 5495
NarI GG'CG_CC
Cuts at: 2926 3174 2926
Size: 248 5247
NgoMIV G'CCGG_C
Cuts at: 3674 3674
Size: 5495
NspI r_CATG'y
Cuts at: 1069 1990 2548 3048 4651 1069
Size: 921 558 500 1603 1913
Fragments arranged by size:
1913 1603 921 558 500
PciI A'CATG_T
Cuts at: 1986 4647 1986
Size: 2661 2834
PflMI CCAn_nnn'nTGG
Cuts at: 886 886
Size: 5495
PfoI T'CCnGG_A
Cuts at: 141 141
Size: 5495
PmeI GTTT'AAAC
Cuts at: 2494 2494
Size: 5495
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3941 3973 3941
Size: 32 5463
PpuMI rG'GwC_Cy
Cuts at: 2250 2250
Size: 5495
PsiI TTA'TAA
Cuts at: 527 527
Size: 5495
PstI C_TGCA'G
Cuts at: 2542 3227 2542
Size: 685 4810
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5495
PvuII CAG'CTG
Cuts at: 2920 3280 2920
Size: 360 5135
RsrII CG'GwC_CG
Cuts at: 3690 3690
Size: 5495
SacI G_AGCT'C
Cuts at: 2509 2509
Size: 5495
SalI G'TCGA_C
Cuts at: 2532 2532
Size: 5495
SapI GCTCTTCn'nnn_
Cuts at: 878 3518 3728 878
Size: 2640 210 2645
Fragments arranged by size:
2645 2640 210
SbfI CC_TGCA'GG
Cuts at: 2542 2542
Size: 5495
ScaI AGT'ACT
Cuts at: 5012 5012
Size: 5495
SfcI C'TryA_G
Cuts at: 33 1095 2538 3223 4191 4382 33
Size: 1062 1443 685 968 191 1146
Fragments arranged by size:
1443 1146 1062 968 685 191
SfoI GGC'GCC
Cuts at: 2927 3175 2927
Size: 248 5247
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5495
SmaI CCC'GGG
Cuts at: 2517 2517
Size: 5495
SmlI C'TyrA_G
Cuts at: 1060 1460 1522 2650 3565 4279 4541 1060
Size: 400 62 1128 915 714 262 2014
Fragments arranged by size:
2014 1128 915 714 400 262 62
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5495
SphI G_CATG'C
Cuts at: 2548 3048 2548
Size: 500 4995
SspI AAT'ATT
Cuts at: 1090 1090
Size: 5495
StyI C'CwwG_G
Cuts at: 2629 2983 2629
Size: 354 5141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1103 3078 3211 3670 3879 1103
Size: 1975 133 459 209 2719
Fragments arranged by size:
2719 1975 459 209 133
TatI w'GTAC_w
Cuts at: 1666 5010 1666
Size: 3344 2151
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1039 1071 5012 5044 1039
Size: 32 3941 32 1490
Fragments arranged by size:
3941 1490 32 32
Tth111I GACn'n_nGTC
Cuts at: 3292 3292
Size: 5495
XbaI T'CTAG_A
Cuts at: 2526 2526
Size: 5495
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2990 2990
Size: 5495
XmaI C'CCGG_G
Cuts at: 2515 2515
Size: 5495
XmnI GAAnn'nnTTC
Cuts at: 2117 2456 2117
Size: 339 5156
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmtI
BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI