Restriction map for
pF1KB9402
(Circular) (Six-base) MAPSORT of: pF1KB9402.seq Check: 8471 from: 1 to: 4838
pF1KB9402 4838 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 129 417 129
Size: 288 4550
AccI GT'mk_AC
Cuts at: 1004 1424 1876 1004
Size: 420 452 3966
Fragments arranged by size:
3966 452 420
Acc65I G'GTAC_C
Cuts at: 1854 1854
Size: 4838
AclI AA'CG_TT
Cuts at: 752 4678 752
Size: 3926 912
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2663 3095 3842 4110 2663
Size: 432 747 268 3391
Fragments arranged by size:
3391 747 432 268
AflIII A'CryG_T
Cuts at: 2190 3294 4271 2190
Size: 1104 977 2757
Fragments arranged by size:
2757 1104 977
AgeI A'CCGG_T
Cuts at: 1830 3226 1830
Size: 1396 3442
AlwNI CAG_nnn'CTG
Cuts at: 1410 1668 2143 3710 1410
Size: 258 475 1567 2538
Fragments arranged by size:
2538 1567 475 258
ApaI G_GGCC'C
Cuts at: 283 283
Size: 4838
ApaLI G'TGCA_C
Cuts at: 430 991 3608 430
Size: 561 2617 1660
Fragments arranged by size:
2617 1660 561
ApoI r'AATT_y
Cuts at: 1842 4191 4447 1842
Size: 2349 256 2233
Fragments arranged by size:
2349 2233 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4838
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4838
AvaI C'yCGr_G
Cuts at: 317 1516 1858 317
Size: 1199 342 3297
Fragments arranged by size:
3297 1199 342
BamHI G'GATC_C
Cuts at: 1863 1863
Size: 4838
BanI G'GyrC_C
Cuts at: 1690 1854 2268 2516 2551 1690
Size: 164 414 248 35 3977
Fragments arranged by size:
3977 414 248 164 35
BanII G_rGCy'C
Cuts at: 283 328 878 943 1852 2882 283
Size: 45 550 65 909 1030 2239
Fragments arranged by size:
2239 1030 909 550 65 45
BbeI G_GCGC'C
Cuts at: 2272 2520 2272
Size: 248 4590
BbsI GAAGACnn'nnnn_
Cuts at: 802 1450 802
Size: 648 4190
BbvCI CC'TCA_GC
Cuts at: 1013 1013
Size: 4838
BciVI GTATCCnnnnn_n'
Cuts at: 2736 3497 4838 2736
Size: 761 1341 2736
Fragments arranged by size:
2736 1341 761
BclI T'GATC_A
Cuts at: 2358 2358
Size: 4838
BglI GCCn_nnn'nGGC
Cuts at: 938 938
Size: 4838
BglII A'GATC_T
Cuts at: 2353 4390 2353
Size: 2037 2801
BlpI GC'TnA_GC
Cuts at: 1950 1950
Size: 4838
Bme1580I G_kGCm'C
Cuts at: 272 283 434 995 2463 2556 3612 272
Size: 11 151 561 1468 93 1056 1498
Fragments arranged by size:
1498 1468 1056 561 151 93 11
BmrI ACTGGGnnnn_n'
Cuts at: 504 615 879 1081 2050 2131 2466 4610 504
Size: 111 264 202 969 81 335 2144 732
Fragments arranged by size:
2144 969 732 335 264 202 111 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3205 3205
Size: 4838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 1199 1414 1432 1621 611
Size: 588 215 18 189 3828
Fragments arranged by size:
3828 588 215 189 18
Bpu10I CC'TnA_GC
Cuts at: 611 1013 611
Size: 402 4436
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2014 2893 3385 3683 2014
Size: 879 492 298 3169
Fragments arranged by size:
3169 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4542 4542
Size: 4838
BsaAI yAC'GTr
Cuts at: 251 2191 251
Size: 1940 2898
BsaBI GATnn'nnATC
Cuts at: 6 193 2352 4389 6
Size: 187 2159 2037 455
Fragments arranged by size:
2159 2037 455 187
BsaHI Gr'CG_yC
Cuts at: 145 2269 2517 3219 4715 145
Size: 2124 248 702 1496 268
Fragments arranged by size:
2124 1496 702 268 248
BsaWI w'CCGG_w
Cuts at: 732 1830 2027 2548 3226 3500 3647 4058 732
Size: 1098 197 521 678 274 147 411 1512
Fragments arranged by size:
1512 1098 678 521 411 274 197 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1085 1115 1539 1569 1085
Size: 30 424 30 4354
Fragments arranged by size:
4354 424 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 488 648 1220 488
Size: 160 572 4106
Fragments arranged by size:
4106 572 160
BseYI C'CCAG_C
Cuts at: 368 452 587 594 770 807 1052 1234 1300
Size: 84 135 7 176 37 245 182 66
Cuts at: 1300 1642 1754 1792 1821 2263 3021 3598 4094
Size: 342 112 38 29 442 758 577 496
Cuts at: 4094 368
Size: 1112
Fragments arranged by size:
1112 758 577 496 442 342 245 182
176 135 112 84 66 38 37 29
7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1025 1025
Size: 4838
BsiEI CG_ry'CG
Cuts at: 104 2380 2426 3634 104
Size: 2276 46 1208 1308
Fragments arranged by size:
2276 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 404 434 995 1852 2630 2820 3612 404
Size: 30 561 857 778 190 792 1630
Fragments arranged by size:
1630 857 792 778 561 190 30
BsmBI CGTCTCn'nnnn_
Cuts at: 1544 1544
Size: 4838
Bsp1286I G_dGCh'C
Cuts at: 272 283 328 404 434 878 943 995 1852
Size: 11 45 76 30 444 65 52 857
Cuts at: 1852 2463 2556 2630 2820 2882 3612 272
Size: 611 93 74 190 62 730 1498
Fragments arranged by size:
1498 857 730 611 444 190 93 76
74 65 62 52 45 30 11
BspEI T'CCGG_A
Cuts at: 4058 4058
Size: 4838
BspHI T'CATG_A
Cuts at: 4014 4014
Size: 4838
BspMI ACCTGCnnnn'nnnn_
Cuts at: 129 417 1888 2102 2404 2785 129
Size: 288 1471 214 302 381 2182
Fragments arranged by size:
2182 1471 381 302 288 214
BsrBI CCG'CTC
Cuts at: 50 615 3130 3184 4668 4833 50
Size: 565 2515 54 1484 165 55
Fragments arranged by size:
2515 1484 565 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2750 2750
Size: 4838
BsrFI r'CCGG_y
Cuts at: 208 422 1830 2836 3017 3226 208
Size: 214 1408 1006 181 209 1820
Fragments arranged by size:
1820 1408 1006 214 209 181
BssSI C'ACGA_G
Cuts at: 742 3109 3467 742
Size: 2367 358 2113
Fragments arranged by size:
2367 2113 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2215 2215
Size: 4838
BstBI TT'CG_AA
Cuts at: 3199 3199
Size: 4838
BstEII G'GTnAC_C
Cuts at: 157 1816 157
Size: 1659 3179
BstXI CCAn_nnnn'nTGG
Cuts at: 1275 1275
Size: 4838
BstYI r'GATC_y
Cuts at: 1 854 1737 1863 2330 2353 2688 2934 3946
Size: 853 883 126 467 23 335 246 1012
Cuts at: 3946 4032 4044 4390 1
Size: 86 12 346 449
Fragments arranged by size:
1012 883 853 467 449 346 335 246
126 86 23 12
BtgI C'CryG_G
Cuts at: 603 712 998 1694 603
Size: 109 286 696 3747
Fragments arranged by size:
3747 696 286 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2788 2969 3132 2788
Size: 181 163 4494
Fragments arranged by size:
4494 181 163
BtsI GCAGTG_nn'
Cuts at: 1304 1666 2081 1304
Size: 362 415 4061
Fragments arranged by size:
4061 415 362
DraI TTT'AAA
Cuts at: 1837 1837
Size: 4838
DraIII CAC_nnn'GTG
Cuts at: 251 360 999 251
Size: 109 639 4090
Fragments arranged by size:
4090 639 109
DrdI GACnn_nn'nnGTC
Cuts at: 2544 3402 2544
Size: 858 3980
EaeI y'GGCC_r
Cuts at: 662 760 2423 2597 2988 3015 4360 662
Size: 98 1663 174 391 27 1345 1140
Fragments arranged by size:
1663 1345 1140 391 174 98 27
EagI C'GGCC_G
Cuts at: 2423 2423
Size: 4838
EarI CTCTTCn'nnn_
Cuts at: 1266 1280 1466 2861 3071 1266
Size: 14 186 1395 210 3033
Fragments arranged by size:
3033 1395 210 186 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3046 3356 3502 4648 3046
Size: 310 146 1146 3236
Fragments arranged by size:
3236 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1850 1850
Size: 4838
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 1199 1414 1432 1621 2663 3095 3842 4110
Size: 588 215 18 189 1042 432 747 268
Cuts at: 4110 611
Size: 1339
Fragments arranged by size:
1339 1042 747 588 432 268 215 189
18
EcoNI CCTnn'n_nnAGG
Cuts at: 681 1396 681
Size: 715 4123
EcoO109I rG'GnC_Cy
Cuts at: 279 280 506 1977 279
Size: 1 226 1471 3140
Fragments arranged by size:
3140 1471 226 1
EcoRI G'AATT_C
Cuts at: 1842 1842
Size: 4838
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2857 2857
Size: 4838
FspI TGC'GCA
Cuts at: 2619 2619
Size: 4838
HaeII r_GCGC'y
Cuts at: 116 416 2272 2520 3542 4521 116
Size: 300 1856 248 1022 979 433
Fragments arranged by size:
1856 1022 979 433 300 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 173 205 280 312 631 663 1085 1117 1541
Size: 32 75 32 319 32 422 32 424
Cuts at: 1541 1573 2680 2710 2712 2742 173
Size: 32 1107 30 2 30 2269
Fragments arranged by size:
2269 1107 424 422 319 75 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1005 1877 1005
Size: 872 3966
KasI G'GCGC_C
Cuts at: 2268 2516 2268
Size: 248 4590
KpnI G_GTAC'C
Cuts at: 1858 1858
Size: 4838
MluI A'CGCG_T
Cuts at: 4271 4271
Size: 4838
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 399 1071 3509 3693 399
Size: 672 2438 184 1544
Fragments arranged by size:
2438 1544 672 184
MscI TGG'CCA
Cuts at: 2599 2599
Size: 4838
MslI CAynn'nnrTG
Cuts at: 397 733 988 1684 2827 2954 397
Size: 336 255 696 1143 127 2281
Fragments arranged by size:
2281 1143 696 336 255 127
MspA1I CmG'CkG
Cuts at: 493 574 631 714 1949 2263 2623 3636 3881
Size: 81 57 83 1235 314 360 1013 245
Cuts at: 3881 493
Size: 1450
Fragments arranged by size:
1450 1235 1013 360 314 245 83 81
57
NaeI GCC'GGC
Cuts at: 3019 3019
Size: 4838
NarI GG'CG_CC
Cuts at: 2269 2517 2269
Size: 248 4590
NgoMIV G'CCGG_C
Cuts at: 3017 3017
Size: 4838
NspI r_CATG'y
Cuts at: 531 1891 2391 3298 531
Size: 1360 500 907 2071
Fragments arranged by size:
2071 1360 907 500
PciI A'CATG_T
Cuts at: 3294 3294
Size: 4838
PflMI CCAn_nnn'nTGG
Cuts at: 251 251
Size: 4838
PfoI T'CCnGG_A
Cuts at: 1107 1365 1391 1107
Size: 258 26 4554
Fragments arranged by size:
4554 258 26
PmeI GTTT'AAAC
Cuts at: 1837 1837
Size: 4838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3977 4009 3977
Size: 32 4806
PpuMI rG'GwC_Cy
Cuts at: 506 506
Size: 4838
PspOMI G'GGCC_C
Cuts at: 279 279
Size: 4838
PstI C_TGCA'G
Cuts at: 1317 1673 1885 2570 1317
Size: 356 212 685 3585
Fragments arranged by size:
3585 685 356 212
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4838
PvuII CAG'CTG
Cuts at: 631 2263 2623 631
Size: 1632 360 2846
Fragments arranged by size:
2846 1632 360
RsrII CG'GwC_CG
Cuts at: 3033 3033
Size: 4838
SacI G_AGCT'C
Cuts at: 1852 1852
Size: 4838
SacII CC_GC'GG
Cuts at: 715 715
Size: 4838
SalI G'TCGA_C
Cuts at: 1003 1875 1003
Size: 872 3966
SapI GCTCTTCn'nnn_
Cuts at: 2861 3071 2861
Size: 210 4628
SbfI CC_TGCA'GG
Cuts at: 1885 1885
Size: 4838
ScaI AGT'ACT
Cuts at: 4355 4355
Size: 4838
SfcI C'TryA_G
Cuts at: 33 1280 1313 1421 1669 1881 2566 3559 3750
Size: 1247 33 108 248 212 685 993 191
Cuts at: 3750 33
Size: 1121
Fragments arranged by size:
1247 1121 993 685 248 212 191 108
33
SfoI GGC'GCC
Cuts at: 2270 2518 2270
Size: 248 4590
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4838
SmaI CCC'GGG
Cuts at: 319 1860 319
Size: 1541 3297
SmlI C'TyrA_G
Cuts at: 1993 2908 3400 3662 1993
Size: 915 492 262 3169
Fragments arranged by size:
3169 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4838
SphI G_CATG'C
Cuts at: 531 1891 2391 531
Size: 1360 500 2978
Fragments arranged by size:
2978 1360 500
StuI AGG'CCT
Cuts at: 1011 1011
Size: 4838
StyI C'CwwG_G
Cuts at: 284 1972 2326 284
Size: 1688 354 2796
Fragments arranged by size:
2796 1688 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 136 1678 2421 2554 3013 3222 136
Size: 1542 743 133 459 209 1752
Fragments arranged by size:
1752 1542 743 459 209 133
TatI w'GTAC_w
Cuts at: 4353 4353
Size: 4838
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1539 1571 4355 4387 1539
Size: 32 2784 32 1990
Fragments arranged by size:
2784 1990 32 32
Tth111I GACn'n_nGTC
Cuts at: 1555 2635 1555
Size: 1080 3758
XbaI T'CTAG_A
Cuts at: 1869 1869
Size: 4838
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 138 1491 2333 138
Size: 1353 842 2643
Fragments arranged by size:
2643 1353 842
XmaI C'CCGG_G
Cuts at: 317 1858 317
Size: 1541 3297
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI