Restriction map for
pF1KB9392
(Circular) (Six-base) MAPSORT of: pF1KB9392.seq Check: 6440 from: 1 to: 5519
pF1KB9392 5519 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1501 1501
Size: 5519
AccI GT'mk_AC
Cuts at: 1604 2004 2557 1604
Size: 400 553 4566
Fragments arranged by size:
4566 553 400
Acc65I G'GTAC_C
Cuts at: 2535 2535
Size: 5519
AclI AA'CG_TT
Cuts at: 5359 5359
Size: 5519
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 998 1664 1862 1994 2018 3344 3776 4129
Size: 376 666 198 132 24 1326 432 353
Cuts at: 4129 4791 622
Size: 662 1350
Fragments arranged by size:
1350 1326 666 662 432 376 353 198
132 24
AfeI AGC'GCT
Cuts at: 2383 2383
Size: 5519
AflIII A'CryG_T
Cuts at: 161 2871 4671 4952 161
Size: 2710 1800 281 728
Fragments arranged by size:
2710 1800 728 281
AlwNI CAG_nnn'CTG
Cuts at: 337 1188 2232 2824 4262 337
Size: 851 1044 592 1438 1594
Fragments arranged by size:
1594 1438 1044 851 592
ApaLI G'TGCA_C
Cuts at: 147 1673 4357 147
Size: 1526 2684 1309
Fragments arranged by size:
2684 1526 1309
ApoI r'AATT_y
Cuts at: 959 1538 2271 2523 4872 5128 959
Size: 579 733 252 2349 256 1350
Fragments arranged by size:
2349 1350 733 579 256 252
AseI AT'TA_AT
Cuts at: 20 20
Size: 5519
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5519
AvaI C'yCGr_G
Cuts at: 2539 2539
Size: 5519
BamHI G'GATC_C
Cuts at: 2544 2544
Size: 5519
BanI G'GyrC_C
Cuts at: 2535 2949 3197 3232 2535
Size: 414 248 35 4822
Fragments arranged by size:
4822 414 248 35
BanII G_rGCy'C
Cuts at: 1047 2533 3563 1047
Size: 1486 1030 3003
Fragments arranged by size:
3003 1486 1030
BbeI G_GCGC'C
Cuts at: 2953 3201 2953
Size: 248 5271
BbsI GAAGACnn'nnnn_
Cuts at: 728 928 728
Size: 200 5319
BciVI GTATCCnnnnn_n'
Cuts at: 107 2027 3417 4473 5519 107
Size: 1920 1390 1056 1046 107
Fragments arranged by size:
1920 1390 1056 1046 107
BclI T'GATC_A
Cuts at: 339 1686 3039 339
Size: 1347 1353 2819
Fragments arranged by size:
2819 1353 1347
BglII A'GATC_T
Cuts at: 3034 5071 3034
Size: 2037 3482
BlpI GC'TnA_GC
Cuts at: 2631 2631
Size: 5519
Bme1580I G_kGCm'C
Cuts at: 151 459 1677 3144 3237 4361 151
Size: 308 1218 1467 93 1124 1309
Fragments arranged by size:
1467 1309 1218 1124 308 93
BmgBI CAC'GTC
Cuts at: 146 162 219 146
Size: 16 57 5446
Fragments arranged by size:
5446 57 16
BmrI ACTGGGnnnn_n'
Cuts at: 1392 2075 2731 2812 3147 5291 1392
Size: 683 656 81 335 2144 1620
Fragments arranged by size:
2144 1620 683 656 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3886 3886
Size: 5519
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1061 1061
Size: 5519
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 915 1309 2695 3574 4288 4586 915
Size: 394 1386 879 714 298 1848
Fragments arranged by size:
1848 1386 879 714 394 298
BsaI GGTCTCn'nnnn_
Cuts at: 1029 1109 5223 1029
Size: 80 4114 1325
Fragments arranged by size:
4114 1325 80
BsaAI yAC'GTr
Cuts at: 586 2872 586
Size: 2286 3233
BsaBI GATnn'nnATC
Cuts at: 6 3033 5070 6
Size: 3027 2037 455
Fragments arranged by size:
3027 2037 455
BsaHI Gr'CG_yC
Cuts at: 2950 3198 3900 5396 2950
Size: 248 702 1496 3073
Fragments arranged by size:
3073 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2708 3229 3907 4318 4465 4739 2708
Size: 521 678 411 147 274 3488
Fragments arranged by size:
3488 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1767 1797 1986 2016 1767
Size: 30 189 30 5270
Fragments arranged by size:
5270 189 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 199 2177 199
Size: 1978 3541
BseYI C'CCAG_C
Cuts at: 2944 3702 4367 4775 2944
Size: 758 665 408 3688
Fragments arranged by size:
3688 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1608 3061 3107 4337 104
Size: 1504 1453 46 1230 1286
Fragments arranged by size:
1504 1453 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 151 1677 2533 3311 3501 4361 151
Size: 1526 856 778 190 860 1309
Fragments arranged by size:
1526 1309 860 856 778 190
BsiWI C'GTAC_G
Cuts at: 1411 1411
Size: 5519
BsmI GAATG_Cn'
Cuts at: 235 235
Size: 5519
Bsp1286I G_dGCh'C
Cuts at: 151 459 1047 1677 2533 3144 3237 3311 3501
Size: 308 588 630 856 611 93 74 190
Cuts at: 3501 3563 4361 151
Size: 62 798 1309
Fragments arranged by size:
1309 856 798 630 611 588 308 190
93 74 62
BspHI T'CATG_A
Cuts at: 2447 3951 2447
Size: 1504 4015
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1501 1965 2014 2569 2783 3085 3466 1501
Size: 464 49 555 214 302 381 3554
Fragments arranged by size:
3554 555 464 381 302 214 49
BsrBI CCG'CTC
Cuts at: 50 198 3811 3865 5349 5514 50
Size: 148 3613 54 1484 165 55
Fragments arranged by size:
3613 1484 165 148 55 54
BsrDI GCAATG_nn'
Cuts at: 3431 3431
Size: 5519
BsrFI r'CCGG_y
Cuts at: 2420 3517 3698 2420
Size: 1097 181 4241
Fragments arranged by size:
4241 1097 181
BsrGI T'GTAC_A
Cuts at: 2138 2138
Size: 5519
BssSI C'ACGA_G
Cuts at: 452 800 3790 4498 452
Size: 348 2990 708 1473
Fragments arranged by size:
2990 1473 708 348
BstAPI GCAn_nnn'nTGC
Cuts at: 2896 2896
Size: 5519
BstBI TT'CG_AA
Cuts at: 3880 3880
Size: 5519
BstEII G'GTnAC_C
Cuts at: 2026 2026
Size: 5519
BstXI CCAn_nnnn'nTGG
Cuts at: 1534 1534
Size: 5519
BstYI r'GATC_y
Cuts at: 1 789 2544 3011 3034 3369 3615 3921 3933
Size: 788 1755 467 23 335 246 306 12
Cuts at: 3933 4019 5071 1
Size: 86 1052 449
Fragments arranged by size:
1755 1052 788 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 1908 1908
Size: 5519
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3469 3650 3813 3469
Size: 181 163 5175
Fragments arranged by size:
5175 181 163
BtsI GCAGTG_nn'
Cuts at: 2762 2762
Size: 5519
DraI TTT'AAA
Cuts at: 1812 2518 1812
Size: 706 4813
DrdI GACnn_nn'nnGTC
Cuts at: 3225 4569 3225
Size: 1344 4175
EaeI y'GGCC_r
Cuts at: 1888 2433 3104 3278 3669 3696 5041 1888
Size: 545 671 174 391 27 1345 2366
Fragments arranged by size:
2366 1345 671 545 391 174 27
EagI C'GGCC_G
Cuts at: 3104 3104
Size: 5519
EarI CTCTTCn'nnn_
Cuts at: 247 1250 1371 1935 3542 3752 247
Size: 1003 121 564 1607 210 2014
Fragments arranged by size:
2014 1607 1003 564 210 121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3727 4469 4615 5329 3727
Size: 742 146 714 3917
Fragments arranged by size:
3917 742 714 146
EcoICRI GAG'CTC
Cuts at: 2531 2531
Size: 5519
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 998 1061 1664 1862 1994 2018 3344 3776
Size: 376 63 603 198 132 24 1326 432
Cuts at: 3776 4129 4791 622
Size: 353 662 1350
Fragments arranged by size:
1350 1326 662 603 432 376 353 198
132 63 24
EcoNI CCTnn'n_nnAGG
Cuts at: 1844 1844
Size: 5519
EcoO109I rG'GnC_Cy
Cuts at: 424 2658 424
Size: 2234 3285
EcoRI G'AATT_C
Cuts at: 1538 2523 1538
Size: 985 4534
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3538 3538
Size: 5519
FspI TGC'GCA
Cuts at: 3300 3300
Size: 5519
HaeII r_GCGC'y
Cuts at: 211 240 1929 2385 2953 3201 4431 5202 211
Size: 29 1689 456 568 248 1230 771 528
Fragments arranged by size:
1689 1230 771 568 528 456 248 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 148 180 565 597 1885 1917 1986 2018 3361
Size: 32 385 32 1288 32 69 32 1343
Cuts at: 3361 3391 3393 3423 148
Size: 30 2 30 2244
Fragments arranged by size:
2244 1343 1288 385 69 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1605 2558 1605
Size: 953 4566
KasI G'GCGC_C
Cuts at: 2949 3197 2949
Size: 248 5271
KpnI G_GTAC'C
Cuts at: 2539 2539
Size: 5519
MfeI C'AATT_G
Cuts at: 2165 2165
Size: 5519
MluI A'CGCG_T
Cuts at: 4952 4952
Size: 5519
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 242 276 1452 4278 4462 242
Size: 34 1176 2826 184 1299
Fragments arranged by size:
2826 1299 1176 184 34
MscI TGG'CCA
Cuts at: 1890 2435 3280 1890
Size: 545 845 4129
Fragments arranged by size:
4129 845 545
MslI CAynn'nnrTG
Cuts at: 1532 3508 3635 1532
Size: 1976 127 3416
Fragments arranged by size:
3416 1976 127
MspA1I CmG'CkG
Cuts at: 318 1684 2630 2944 3304 4088 4333 318
Size: 1366 946 314 360 784 245 1504
Fragments arranged by size:
1504 1366 946 784 360 314 245
NaeI GCC'GGC
Cuts at: 2422 3700 2422
Size: 1278 4241
NarI GG'CG_CC
Cuts at: 2950 3198 2950
Size: 248 5271
NcoI C'CATG_G
Cuts at: 1908 1908
Size: 5519
NgoMIV G'CCGG_C
Cuts at: 2420 3698 2420
Size: 1278 4241
NspI r_CATG'y
Cuts at: 1182 2572 3072 4675 1182
Size: 1390 500 1603 2026
Fragments arranged by size:
2026 1603 1390 500
PciI A'CATG_T
Cuts at: 4671 4671
Size: 5519
PfoI T'CCnGG_A
Cuts at: 876 876
Size: 5519
PmeI GTTT'AAAC
Cuts at: 2518 2518
Size: 5519
PmlI CAC'GTG
Cuts at: 586 586
Size: 5519
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3965 3997 3965
Size: 32 5487
PpuMI rG'GwC_Cy
Cuts at: 424 424
Size: 5519
PsiI TTA'TAA
Cuts at: 863 1871 863
Size: 1008 4511
PstI C_TGCA'G
Cuts at: 2471 2566 3251 2471
Size: 95 685 4739
Fragments arranged by size:
4739 685 95
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5519
PvuII CAG'CTG
Cuts at: 318 1684 2944 3304 318
Size: 1366 1260 360 2533
Fragments arranged by size:
2533 1366 1260 360
RsrII CG'GwC_CG
Cuts at: 3714 3714
Size: 5519
SacI G_AGCT'C
Cuts at: 2533 2533
Size: 5519
SalI G'TCGA_C
Cuts at: 1603 2556 1603
Size: 953 4566
SapI GCTCTTCn'nnn_
Cuts at: 3542 3752 3542
Size: 210 5309
SbfI CC_TGCA'GG
Cuts at: 2566 2566
Size: 5519
ScaI AGT'ACT
Cuts at: 1365 5036 1365
Size: 3671 1848
SfcI C'TryA_G
Cuts at: 33 1010 2377 2467 2499 2562 3247 4215 4406
Size: 977 1367 90 32 63 685 968 191
Cuts at: 4406 33
Size: 1146
Fragments arranged by size:
1367 1146 977 968 685 191 90 63
32
SfoI GGC'GCC
Cuts at: 2951 3199 2951
Size: 248 5271
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5519
SmaI CCC'GGG
Cuts at: 2541 2541
Size: 5519
SmlI C'TyrA_G
Cuts at: 930 1324 2674 3589 4303 4565 930
Size: 394 1350 915 714 262 1884
Fragments arranged by size:
1884 1350 915 714 394 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5519
SphI G_CATG'C
Cuts at: 2572 3072 2572
Size: 500 5019
StyI C'CwwG_G
Cuts at: 525 1908 1963 2653 3007 525
Size: 1383 55 690 354 3037
Fragments arranged by size:
3037 1383 690 354 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1622 3102 3235 3694 3903 1622
Size: 1480 133 459 209 3238
Fragments arranged by size:
3238 1480 459 209 133
TatI w'GTAC_w
Cuts at: 1363 1423 1575 2138 5034 1363
Size: 60 152 563 2896 1848
Fragments arranged by size:
2896 1848 563 152 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5036 5068 5036
Size: 32 5487
Tth111I GACn'n_nGTC
Cuts at: 3316 3316
Size: 5519
XbaI T'CTAG_A
Cuts at: 771 2550 771
Size: 1779 3740
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3014 3014
Size: 5519
XmaI C'CCGG_G
Cuts at: 2539 2539
Size: 5519
XmnI GAAnn'nnTTC
Cuts at: 961 961
Size: 5519
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PmlI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
Bpu10I BsgI BsmBI BspEI BssHII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI