Restriction map for
pF1KB9390
(Circular) (Six-base) MAPSORT of: pF1KB9390.seq Check: 3494 from: 1 to: 3848
pF1KB9390 3848 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 886 886
Size: 3848
Acc65I G'GTAC_C
Cuts at: 864 864
Size: 3848
AclI AA'CG_TT
Cuts at: 3688 3688
Size: 3848
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 485 715 1673 2105 2458 3120 263
Size: 222 230 958 432 353 662 991
Fragments arranged by size:
991 958 662 432 353 230 222
AflIII A'CryG_T
Cuts at: 1200 3000 3281 1200
Size: 1800 281 1767
Fragments arranged by size:
1800 1767 281
AlwNI CAG_nnn'CTG
Cuts at: 336 1153 2591 336
Size: 817 1438 1593
Fragments arranged by size:
1593 1438 817
ApaLI G'TGCA_C
Cuts at: 2686 2686
Size: 3848
ApoI r'AATT_y
Cuts at: 425 852 3201 3457 425
Size: 427 2349 256 816
Fragments arranged by size:
2349 816 427 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3848
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
AvaI C'yCGr_G
Cuts at: 710 868 710
Size: 158 3690
BamHI G'GATC_C
Cuts at: 873 873
Size: 3848
BanI G'GyrC_C
Cuts at: 193 864 1278 1526 1561 193
Size: 671 414 248 35 2480
Fragments arranged by size:
2480 671 414 248 35
BanII G_rGCy'C
Cuts at: 402 554 862 1892 402
Size: 152 308 1030 2358
Fragments arranged by size:
2358 1030 308 152
BbeI G_GCGC'C
Cuts at: 1282 1530 1282
Size: 248 3600
BbsI GAAGACnn'nnnn_
Cuts at: 659 659
Size: 3848
BbvCI CC'TCA_GC
Cuts at: 179 229 179
Size: 50 3798
BciVI GTATCCnnnnn_n'
Cuts at: 1746 2802 3848 1746
Size: 1056 1046 1746
Fragments arranged by size:
1746 1056 1046
BclI T'GATC_A
Cuts at: 1368 1368
Size: 3848
BglII A'GATC_T
Cuts at: 1363 3400 1363
Size: 2037 1811
BlpI GC'TnA_GC
Cuts at: 548 960 548
Size: 412 3436
Bme1580I G_kGCm'C
Cuts at: 196 306 1473 1566 2690 196
Size: 110 1167 93 1124 1354
Fragments arranged by size:
1354 1167 1124 110 93
BmrI ACTGGGnnnn_n'
Cuts at: 1060 1141 1476 3620 1060
Size: 81 335 2144 1288
Fragments arranged by size:
2144 1288 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 657 2215 657
Size: 1558 2290
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 358 640 148
Size: 210 282 3356
Fragments arranged by size:
3356 282 210
Bpu10I CC'TnA_GC
Cuts at: 179 229 626 179
Size: 50 397 3401
Fragments arranged by size:
3401 397 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 1024 1903 2617 2915 811
Size: 213 879 714 298 1744
Fragments arranged by size:
1744 879 714 298 213
BsaI GGTCTCn'nnnn_
Cuts at: 229 3552 229
Size: 3323 525
BsaAI yAC'GTr
Cuts at: 1201 1201
Size: 3848
BsaBI GATnn'nnATC
Cuts at: 6 1362 3399 6
Size: 1356 2037 455
Fragments arranged by size:
2037 1356 455
BsaHI Gr'CG_yC
Cuts at: 173 1279 1527 2229 3725 173
Size: 1106 248 702 1496 296
Fragments arranged by size:
1496 1106 702 296 248
BsaWI w'CCGG_w
Cuts at: 149 1037 1558 2236 2647 2794 3068 149
Size: 888 521 678 411 147 274 929
Fragments arranged by size:
929 888 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 196 553 196
Size: 357 3491
BseYI C'CCAG_C
Cuts at: 1273 2031 2696 3104 1273
Size: 758 665 408 2017
Fragments arranged by size:
2017 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 554 554
Size: 3848
BsiEI CG_ry'CG
Cuts at: 104 477 1390 1436 2666 104
Size: 373 913 46 1230 1286
Fragments arranged by size:
1286 1230 913 373 46
BsiHKAI G_wGCw'C
Cuts at: 554 862 1640 1830 2690 554
Size: 308 778 190 860 1712
Fragments arranged by size:
1712 860 778 308 190
Bsp1286I G_dGCh'C
Cuts at: 196 306 402 554 862 1473 1566 1640 1830
Size: 110 96 152 308 611 93 74 190
Cuts at: 1830 1892 2690 196
Size: 62 798 1354
Fragments arranged by size:
1354 798 611 308 190 152 110 96
93 74 62
BspEI T'CCGG_A
Cuts at: 149 149
Size: 3848
BspHI T'CATG_A
Cuts at: 2280 2280
Size: 3848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 898 1112 1414 1795 898
Size: 214 302 381 2951
Fragments arranged by size:
2951 381 302 214
BsrBI CCG'CTC
Cuts at: 50 543 2140 2194 3678 3843 50
Size: 493 1597 54 1484 165 55
Fragments arranged by size:
1597 1484 493 165 55 54
BsrDI GCAATG_nn'
Cuts at: 458 1760 458
Size: 1302 2546
BsrFI r'CCGG_y
Cuts at: 1846 2027 1846
Size: 181 3667
BssHII G'CGCG_C
Cuts at: 186 186
Size: 3848
BssSI C'ACGA_G
Cuts at: 217 2119 2827 217
Size: 1902 708 1238
Fragments arranged by size:
1902 1238 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1225 1225
Size: 3848
BstBI TT'CG_AA
Cuts at: 2209 2209
Size: 3848
BstYI r'GATC_y
Cuts at: 1 146 434 873 1340 1363 1698 1944 2250
Size: 145 288 439 467 23 335 246 306
Cuts at: 2250 2262 2348 3400 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 467 449 439 335 306 288 246
145 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3848
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1798 1979 2142 1798
Size: 181 163 3504
Fragments arranged by size:
3504 181 163
BtsI GCAGTG_nn'
Cuts at: 1091 1091
Size: 3848
DraI TTT'AAA
Cuts at: 847 847
Size: 3848
DrdI GACnn_nn'nnGTC
Cuts at: 1554 2898 1554
Size: 1344 2504
EaeI y'GGCC_r
Cuts at: 1433 1607 1998 2025 3370 1433
Size: 174 391 27 1345 1911
Fragments arranged by size:
1911 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1433 1433
Size: 3848
EarI CTCTTCn'nnn_
Cuts at: 284 482 662 1871 2081 284
Size: 198 180 1209 210 2051
Fragments arranged by size:
2051 1209 210 198 180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 184 2056 2798 2944 3658 184
Size: 1872 742 146 714 374
Fragments arranged by size:
1872 742 714 374 146
EcoICRI GAG'CTC
Cuts at: 552 860 552
Size: 308 3540
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 263 358 485 640 715 1673 2105 2458
Size: 115 95 127 155 75 958 432 353
Cuts at: 2458 3120 148
Size: 662 876
Fragments arranged by size:
958 876 662 432 353 155 127 115
95 75
EcoNI CCTnn'n_nnAGG
Cuts at: 835 835
Size: 3848
EcoO109I rG'GnC_Cy
Cuts at: 678 987 678
Size: 309 3539
EcoRI G'AATT_C
Cuts at: 852 852
Size: 3848
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1867 1867
Size: 3848
FspI TGC'GCA
Cuts at: 139 1629 139
Size: 1490 2358
HaeII r_GCGC'y
Cuts at: 1282 1530 2760 3531 1282
Size: 248 1230 771 1599
Fragments arranged by size:
1599 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1690 1720 1722 1752 1690
Size: 30 2 30 3786
Fragments arranged by size:
3786 30 30 2
HincII GTy'rAC
Cuts at: 887 887
Size: 3848
KasI G'GCGC_C
Cuts at: 1278 1526 1278
Size: 248 3600
KpnI G_GTAC'C
Cuts at: 868 868
Size: 3848
MluI A'CGCG_T
Cuts at: 3281 3281
Size: 3848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 275 462 710 2607 2791 275
Size: 187 248 1897 184 1332
Fragments arranged by size:
1897 1332 248 187 184
MscI TGG'CCA
Cuts at: 1609 1609
Size: 3848
MslI CAynn'nnrTG
Cuts at: 1837 1964 1837
Size: 127 3721
MspA1I CmG'CkG
Cuts at: 178 241 279 959 1273 1633 2417 2662 178
Size: 63 38 680 314 360 784 245 1364
Fragments arranged by size:
1364 784 680 360 314 245 63 38
NaeI GCC'GGC
Cuts at: 2029 2029
Size: 3848
NarI GG'CG_CC
Cuts at: 1279 1527 1279
Size: 248 3600
NcoI C'CATG_G
Cuts at: 107 107
Size: 3848
NgoMIV G'CCGG_C
Cuts at: 2027 2027
Size: 3848
NspI r_CATG'y
Cuts at: 901 1401 3004 901
Size: 500 1603 1745
Fragments arranged by size:
1745 1603 500
PciI A'CATG_T
Cuts at: 3000 3000
Size: 3848
PmeI GTTT'AAAC
Cuts at: 847 847
Size: 3848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2294 2326 2294
Size: 32 3816
PpuMI rG'GwC_Cy
Cuts at: 678 678
Size: 3848
PstI C_TGCA'G
Cuts at: 161 203 895 1580 161
Size: 42 692 685 2429
Fragments arranged by size:
2429 692 685 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3848
PvuII CAG'CTG
Cuts at: 279 1273 1633 279
Size: 994 360 2494
Fragments arranged by size:
2494 994 360
RsrII CG'GwC_CG
Cuts at: 2043 2043
Size: 3848
SacI G_AGCT'C
Cuts at: 554 862 554
Size: 308 3540
SalI G'TCGA_C
Cuts at: 885 885
Size: 3848
SapI GCTCTTCn'nnn_
Cuts at: 1871 2081 1871
Size: 210 3638
SbfI CC_TGCA'GG
Cuts at: 161 895 161
Size: 734 3114
ScaI AGT'ACT
Cuts at: 640 3365 640
Size: 2725 1123
SfcI C'TryA_G
Cuts at: 33 157 199 891 1576 2544 2735 33
Size: 124 42 692 685 968 191 1146
Fragments arranged by size:
1146 968 692 685 191 124 42
SfoI GGC'GCC
Cuts at: 1280 1528 1280
Size: 248 3600
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
SmaI CCC'GGG
Cuts at: 870 870
Size: 3848
SmlI C'TyrA_G
Cuts at: 790 1003 1918 2632 2894 790
Size: 213 915 714 262 1744
Fragments arranged by size:
1744 915 714 262 213
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3848
SphI G_CATG'C
Cuts at: 901 1401 901
Size: 500 3348
StyI C'CwwG_G
Cuts at: 107 982 1336 107
Size: 875 354 2619
Fragments arranged by size:
2619 875 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1431 1564 2023 2232 1431
Size: 133 459 209 3047
Fragments arranged by size:
3047 459 209 133
TatI w'GTAC_w
Cuts at: 638 3363 638
Size: 2725 1123
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3365 3397 3365
Size: 32 3816
Tth111I GACn'n_nGTC
Cuts at: 1645 1645
Size: 3848
XbaI T'CTAG_A
Cuts at: 879 879
Size: 3848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1343 1343
Size: 3848
XmaI C'CCGG_G
Cuts at: 868 868
Size: 3848
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BcgI BfrBI BglI
BmgBI BmtI BsaXI BsiWI BsmI BsmBI BsrGI BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI