Restriction map for
pF1KB9385
(Circular) (Six-base) MAPSORT of: pF1KB9385.seq Check: 7163 from: 1 to: 4085
pF1KB9385 4085 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1123 1123
Size: 4085
Acc65I G'GTAC_C
Cuts at: 929 1101 929
Size: 172 3913
AclI AA'CG_TT
Cuts at: 3925 3925
Size: 4085
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 942 1049 1910 2342 3089 3357 942
Size: 107 861 432 747 268 1670
Fragments arranged by size:
1670 861 747 432 268 107
AflIII A'CryG_T
Cuts at: 215 1437 2541 3518 215
Size: 1222 1104 977 782
Fragments arranged by size:
1222 1104 977 782
AgeI A'CCGG_T
Cuts at: 112 2473 112
Size: 2361 1724
AleI CACnn'nnGTG
Cuts at: 220 220
Size: 4085
AlwNI CAG_nnn'CTG
Cuts at: 195 544 1390 2957 195
Size: 349 846 1567 1323
Fragments arranged by size:
1567 1323 846 349
ApaLI G'TGCA_C
Cuts at: 2855 2855
Size: 4085
ApoI r'AATT_y
Cuts at: 1089 3438 3694 1089
Size: 2349 256 1480
Fragments arranged by size:
2349 1480 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4085
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4085
AvaI C'yCGr_G
Cuts at: 145 1105 145
Size: 960 3125
BamHI G'GATC_C
Cuts at: 477 891 1110 477
Size: 414 219 3452
Fragments arranged by size:
3452 414 219
BanI G'GyrC_C
Cuts at: 277 929 1101 1515 1763 1798 277
Size: 652 172 414 248 35 2564
Fragments arranged by size:
2564 652 414 248 172 35
BanII G_rGCy'C
Cuts at: 1099 2129 1099
Size: 1030 3055
BbeI G_GCGC'C
Cuts at: 281 1519 1767 281
Size: 1238 248 2599
Fragments arranged by size:
2599 1238 248
BciVI GTATCCnnnnn_n'
Cuts at: 1983 2744 4085 1983
Size: 761 1341 1983
Fragments arranged by size:
1983 1341 761
BclI T'GATC_A
Cuts at: 366 1605 366
Size: 1239 2846
BfrBI ATG'CAT
Cuts at: 351 351
Size: 4085
BglII A'GATC_T
Cuts at: 1600 3637 1600
Size: 2037 2048
BlpI GC'TnA_GC
Cuts at: 1197 1197
Size: 4085
Bme1580I G_kGCm'C
Cuts at: 1710 1803 2859 1710
Size: 93 1056 2936
Fragments arranged by size:
2936 1056 93
BmgBI CAC'GTC
Cuts at: 218 218
Size: 4085
BmrI ACTGGGnnnn_n'
Cuts at: 183 808 1297 1378 1713 3857 183
Size: 625 489 81 335 2144 411
Fragments arranged by size:
2144 625 489 411 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2452 2452
Size: 4085
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 339 566 339
Size: 227 3858
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 1261 2140 2632 2930 244
Size: 1017 879 492 298 1399
Fragments arranged by size:
1399 1017 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 542 3789 542
Size: 3247 838
BsaAI yAC'GTr
Cuts at: 615 1438 615
Size: 823 3262
BsaBI GATnn'nnATC
Cuts at: 6 1599 3636 6
Size: 1593 2037 455
Fragments arranged by size:
2037 1593 455
BsaHI Gr'CG_yC
Cuts at: 278 1516 1764 2466 3962 278
Size: 1238 248 702 1496 401
Fragments arranged by size:
1496 1238 702 401 248
BsaWI w'CCGG_w
Cuts at: 112 1274 1795 2473 2747 2894 3305 112
Size: 1162 521 678 274 147 411 892
Fragments arranged by size:
1162 892 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 150 195 150
Size: 45 4040
BseYI C'CCAG_C
Cuts at: 499 1510 2268 2845 3341 499
Size: 1011 758 577 496 1243
Fragments arranged by size:
1243 1011 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 637 637
Size: 4085
BsiEI CG_ry'CG
Cuts at: 104 1627 1673 2881 104
Size: 1523 46 1208 1308
Fragments arranged by size:
1523 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1099 1877 2067 2859 1099
Size: 778 190 792 2325
Fragments arranged by size:
2325 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 232 232
Size: 4085
Bsp1286I G_dGCh'C
Cuts at: 1099 1710 1803 1877 2067 2129 2859 1099
Size: 611 93 74 190 62 730 2325
Fragments arranged by size:
2325 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3305 3305
Size: 4085
BspHI T'CATG_A
Cuts at: 3261 3261
Size: 4085
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1135 1349 1651 2032 1135
Size: 214 302 381 3188
Fragments arranged by size:
3188 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2377 2431 3915 4080 50
Size: 2327 54 1484 165 55
Fragments arranged by size:
2327 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1997 1997
Size: 4085
BsrFI r'CCGG_y
Cuts at: 112 240 274 551 2083 2264 2473 112
Size: 128 34 277 1532 181 209 1724
Fragments arranged by size:
1724 1532 277 209 181 128 34
BssSI C'ACGA_G
Cuts at: 936 2356 2714 936
Size: 1420 358 2307
Fragments arranged by size:
2307 1420 358
BstAPI GCAn_nnn'nTGC
Cuts at: 952 1462 952
Size: 510 3575
BstBI TT'CG_AA
Cuts at: 2446 2446
Size: 4085
BstYI r'GATC_y
Cuts at: 1 477 891 1110 1577 1600 1935 2181 3193
Size: 476 414 219 467 23 335 246 1012
Cuts at: 3193 3279 3291 3637 1
Size: 86 12 346 449
Fragments arranged by size:
1012 476 467 449 414 346 335 246
219 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 743 743
Size: 4085
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 927 2035 2216 2379 927
Size: 1108 181 163 2633
Fragments arranged by size:
2633 1108 181 163
BtsI GCAGTG_nn'
Cuts at: 1328 1328
Size: 4085
DraI TTT'AAA
Cuts at: 1084 1084
Size: 4085
DrdI GACnn_nn'nnGTC
Cuts at: 1791 2649 1791
Size: 858 3227
EaeI y'GGCC_r
Cuts at: 242 1670 1844 2235 2262 3607 242
Size: 1428 174 391 27 1345 720
Fragments arranged by size:
1428 1345 720 391 174 27
EagI C'GGCC_G
Cuts at: 1670 1670
Size: 4085
EarI CTCTTCn'nnn_
Cuts at: 2108 2318 2108
Size: 210 3875
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2293 2603 2749 3895 2293
Size: 310 146 1146 2483
Fragments arranged by size:
2483 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1097 1097
Size: 4085
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 339 566 942 1049 1910 2342 3089 3357 339
Size: 227 376 107 861 432 747 268 1067
Fragments arranged by size:
1067 861 747 432 376 268 227 107
EcoO109I rG'GnC_Cy
Cuts at: 386 503 1224 386
Size: 117 721 3247
Fragments arranged by size:
3247 721 117
EcoRI G'AATT_C
Cuts at: 1089 1089
Size: 4085
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 394 783 2104 394
Size: 389 1321 2375
Fragments arranged by size:
2375 1321 389
FspI TGC'GCA
Cuts at: 1866 1866
Size: 4085
HaeII r_GCGC'y
Cuts at: 281 1519 1767 2789 3768 281
Size: 1238 248 1022 979 598
Fragments arranged by size:
1238 1022 979 598 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 142 174 495 527 1927 1957 1959 1989 142
Size: 32 321 32 1400 30 2 30 2238
Fragments arranged by size:
2238 1400 321 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1124 1124
Size: 4085
KasI G'GCGC_C
Cuts at: 277 1515 1763 277
Size: 1238 248 2599
Fragments arranged by size:
2599 1238 248
KpnI G_GTAC'C
Cuts at: 933 1105 933
Size: 172 3913
MfeI C'AATT_G
Cuts at: 340 340
Size: 4085
MluI A'CGCG_T
Cuts at: 3518 3518
Size: 4085
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 331 2756 2940 331
Size: 2425 184 1476
Fragments arranged by size:
2425 1476 184
MscI TGG'CCA
Cuts at: 1846 1846
Size: 4085
MslI CAynn'nnrTG
Cuts at: 220 2074 2201 220
Size: 1854 127 2104
Fragments arranged by size:
2104 1854 127
MspA1I CmG'CkG
Cuts at: 1196 1510 1870 2883 3128 1196
Size: 314 360 1013 245 2153
Fragments arranged by size:
2153 1013 360 314 245
NaeI GCC'GGC
Cuts at: 242 2266 242
Size: 2024 2061
NarI GG'CG_CC
Cuts at: 278 1516 1764 278
Size: 1238 248 2599
Fragments arranged by size:
2599 1238 248
NgoMIV G'CCGG_C
Cuts at: 240 2264 240
Size: 2024 2061
NsiI A_TGCA'T
Cuts at: 353 353
Size: 4085
NspI r_CATG'y
Cuts at: 316 1138 1638 2545 316
Size: 822 500 907 1856
Fragments arranged by size:
1856 907 822 500
PciI A'CATG_T
Cuts at: 2541 2541
Size: 4085
PfoI T'CCnGG_A
Cuts at: 284 284
Size: 4085
PmeI GTTT'AAAC
Cuts at: 1084 1084
Size: 4085
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3224 3256 3224
Size: 32 4053
PpuMI rG'GwC_Cy
Cuts at: 386 503 386
Size: 117 3968
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 595 627 595
Size: 32 4053
PstI C_TGCA'G
Cuts at: 1132 1817 1132
Size: 685 3400
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4085
PvuII CAG'CTG
Cuts at: 1510 1870 1510
Size: 360 3725
RsrII CG'GwC_CG
Cuts at: 2280 2280
Size: 4085
SacI G_AGCT'C
Cuts at: 1099 1099
Size: 4085
SalI G'TCGA_C
Cuts at: 1122 1122
Size: 4085
SanDI GG'GwC_CC
Cuts at: 386 386
Size: 4085
SapI GCTCTTCn'nnn_
Cuts at: 2108 2318 2108
Size: 210 3875
SbfI CC_TGCA'GG
Cuts at: 1132 1132
Size: 4085
ScaI AGT'ACT
Cuts at: 3602 3602
Size: 4085
SfcI C'TryA_G
Cuts at: 33 1128 1813 2806 2997 33
Size: 1095 685 993 191 1121
Fragments arranged by size:
1121 1095 993 685 191
SfoI GGC'GCC
Cuts at: 279 1517 1765 279
Size: 1238 248 2599
Fragments arranged by size:
2599 1238 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4085
SgrAI Cr'CCGG_yG
Cuts at: 274 274
Size: 4085
SmaI CCC'GGG
Cuts at: 1107 1107
Size: 4085
SmlI C'TyrA_G
Cuts at: 259 1240 2155 2647 2909 259
Size: 981 915 492 262 1435
Fragments arranged by size:
1435 981 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4085
SphI G_CATG'C
Cuts at: 316 1138 1638 316
Size: 822 500 2763
Fragments arranged by size:
2763 822 500
SspI AAT'ATT
Cuts at: 798 885 798
Size: 87 3998
StyI C'CwwG_G
Cuts at: 1076 1219 1573 1076
Size: 143 354 3588
Fragments arranged by size:
3588 354 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1668 1801 2260 2469 1668
Size: 133 459 209 3284
Fragments arranged by size:
3284 459 209 133
TatI w'GTAC_w
Cuts at: 3600 3600
Size: 4085
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1008 1040 3602 3634 1008
Size: 32 2562 32 1459
Fragments arranged by size:
2562 1459 32 32
Tth111I GACn'n_nGTC
Cuts at: 1882 1882
Size: 4085
XbaI T'CTAG_A
Cuts at: 1116 1116
Size: 4085
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 314 1580 314
Size: 1266 2819
XmaI C'CCGG_G
Cuts at: 1105 1105
Size: 4085
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI
NspI PciI PfoI PmeI PpiI PpuMI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmtI
Bpu10I BsaXI BsiWI BsmI BsrGI BssHII BstEII BstXI
BstZ17I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI NcoI NdeI NheI NotI NruI
PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI
SacII SexAI SfiI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI