Restriction map for
pF1KB9376
(Circular) (Six-base) MAPSORT of: pF1KB9376.seq Check: 3384 from: 1 to: 4346
pF1KB9376 4346 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 1384 1384
Size: 4346
Acc65I G'GTAC_C
Cuts at: 1362 1362
Size: 4346
AclI AA'CG_TT
Cuts at: 4186 4186
Size: 4346
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 839 1015 1223 2171 2603 3350 3618 515
Size: 324 176 208 948 432 747 268 1243
Fragments arranged by size:
1243 948 747 432 324 268 208 176
AflIII A'CryG_T
Cuts at: 1698 2802 3779 1698
Size: 1104 977 2265
Fragments arranged by size:
2265 1104 977
AgeI A'CCGG_T
Cuts at: 2734 2734
Size: 4346
AleI CACnn'nnGTG
Cuts at: 718 718
Size: 4346
AlwNI CAG_nnn'CTG
Cuts at: 766 1067 1126 1651 3218 766
Size: 301 59 525 1567 1894
Fragments arranged by size:
1894 1567 525 301 59
ApaLI G'TGCA_C
Cuts at: 3116 3116
Size: 4346
ApoI r'AATT_y
Cuts at: 252 408 506 539 1350 3699 3955 252
Size: 156 98 33 811 2349 256 643
Fragments arranged by size:
2349 811 643 256 156 98 33
AseI AT'TA_AT
Cuts at: 20 20
Size: 4346
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4346
AvaI C'yCGr_G
Cuts at: 1366 1366
Size: 4346
BamHI G'GATC_C
Cuts at: 1371 1371
Size: 4346
BanI G'GyrC_C
Cuts at: 699 1362 1776 2024 2059 699
Size: 663 414 248 35 2986
Fragments arranged by size:
2986 663 414 248 35
BanII G_rGCy'C
Cuts at: 142 149 1360 2390 142
Size: 7 1211 1030 2098
Fragments arranged by size:
2098 1211 1030 7
BbeI G_GCGC'C
Cuts at: 1780 2028 1780
Size: 248 4098
BciVI GTATCCnnnnn_n'
Cuts at: 2244 3005 4346 2244
Size: 761 1341 2244
Fragments arranged by size:
2244 1341 761
BclI T'GATC_A
Cuts at: 351 1866 351
Size: 1515 2831
BfrBI ATG'CAT
Cuts at: 190 190
Size: 4346
BglII A'GATC_T
Cuts at: 1861 3898 1861
Size: 2037 2309
BlpI GC'TnA_GC
Cuts at: 1458 1458
Size: 4346
Bme1580I G_kGCm'C
Cuts at: 1971 2064 3120 1971
Size: 93 1056 3197
Fragments arranged by size:
3197 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 621 1077 1558 1639 1974 4118 621
Size: 456 481 81 335 2144 849
Fragments arranged by size:
2144 849 481 456 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2713 2713
Size: 4346
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1148 1148
Size: 4346
Bpu10I CC'TnA_GC
Cuts at: 141 141
Size: 4346
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1522 2401 2893 3191 1522
Size: 879 492 298 2677
Fragments arranged by size:
2677 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4050 4050
Size: 4346
BsaAI yAC'GTr
Cuts at: 1699 1699
Size: 4346
BsaBI GATnn'nnATC
Cuts at: 6 1860 3897 6
Size: 1854 2037 455
Fragments arranged by size:
2037 1854 455
BsaHI Gr'CG_yC
Cuts at: 1777 2025 2727 4223 1777
Size: 248 702 1496 1900
Fragments arranged by size:
1900 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1535 2056 2734 3008 3155 3566 1535
Size: 521 678 274 147 411 2315
Fragments arranged by size:
2315 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 729 759 729
Size: 30 4316
BseYI C'CCAG_C
Cuts at: 1771 2529 3106 3602 1771
Size: 758 577 496 2515
Fragments arranged by size:
2515 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 397
Size: 4346
BsiEI CG_ry'CG
Cuts at: 104 1888 1934 3142 104
Size: 1784 46 1208 1308
Fragments arranged by size:
1784 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 658 1360 2138 2328 3120 658
Size: 702 778 190 792 1884
Fragments arranged by size:
1884 792 778 702 190
BsmBI CGTCTCn'nnnn_
Cuts at: 453 453
Size: 4346
Bsp1286I G_dGCh'C
Cuts at: 142 149 658 1360 1971 2064 2138 2328 2390
Size: 7 509 702 611 93 74 190 62
Cuts at: 2390 3120 142
Size: 730 1368
Fragments arranged by size:
1368 730 702 611 509 190 93 74
62 7
BspEI T'CCGG_A
Cuts at: 3566 3566
Size: 4346
BspHI T'CATG_A
Cuts at: 3522 3522
Size: 4346
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1396 1610 1912 2293 1396
Size: 214 302 381 3449
Fragments arranged by size:
3449 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2638 2692 4176 4341 50
Size: 2588 54 1484 165 55
Fragments arranged by size:
2588 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 798 1043 2258 798
Size: 245 1215 2886
Fragments arranged by size:
2886 1215 245
BsrFI r'CCGG_y
Cuts at: 2344 2525 2734 2344
Size: 181 209 3956
Fragments arranged by size:
3956 209 181
BsrGI T'GTAC_A
Cuts at: 624 624
Size: 4346
BssSI C'ACGA_G
Cuts at: 2617 2975 2617
Size: 358 3988
BstAPI GCAn_nnn'nTGC
Cuts at: 1723 1723
Size: 4346
BstBI TT'CG_AA
Cuts at: 2707 2707
Size: 4346
BstYI r'GATC_y
Cuts at: 1 1371 1838 1861 2196 2442 3454 3540 3552
Size: 1370 467 23 335 246 1012 86 12
Cuts at: 3552 3898 1
Size: 346 449
Fragments arranged by size:
1370 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 312 815 107
Size: 205 503 3638
Fragments arranged by size:
3638 503 205
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2296 2477 2640 2296
Size: 181 163 4002
Fragments arranged by size:
4002 181 163
BtsI GCAGTG_nn'
Cuts at: 380 911 1256 1589 380
Size: 531 345 333 3137
Fragments arranged by size:
3137 531 345 333
DraI TTT'AAA
Cuts at: 1345 1345
Size: 4346
DrdI GACnn_nn'nnGTC
Cuts at: 2052 2910 2052
Size: 858 3488
EaeI y'GGCC_r
Cuts at: 530 1931 2105 2496 2523 3868 530
Size: 1401 174 391 27 1345 1008
Fragments arranged by size:
1401 1345 1008 391 174 27
EagI C'GGCC_G
Cuts at: 1931 1931
Size: 4346
EarI CTCTTCn'nnn_
Cuts at: 130 2369 2579 130
Size: 2239 210 1897
Fragments arranged by size:
2239 1897 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 109 2554 2864 3010 4156 109
Size: 2445 310 146 1146 299
Fragments arranged by size:
2445 1146 310 299 146
EcoICRI GAG'CTC
Cuts at: 1358 1358
Size: 4346
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 839 1015 1148 1223 2171 2603 3350 3618
Size: 324 176 133 75 948 432 747 268
Cuts at: 3618 515
Size: 1243
Fragments arranged by size:
1243 948 747 432 324 268 176 133
75
EcoO109I rG'GnC_Cy
Cuts at: 1485 1485
Size: 4346
EcoRI G'AATT_C
Cuts at: 506 539 1350 506
Size: 33 811 3502
Fragments arranged by size:
3502 811 33
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 526 2365 526
Size: 1839 2507
FspI TGC'GCA
Cuts at: 2127 2127
Size: 4346
HaeII r_GCGC'y
Cuts at: 1780 2028 3050 4029 1780
Size: 248 1022 979 2097
Fragments arranged by size:
2097 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 433 465 2188 2218 2220 2250 433
Size: 32 1723 30 2 30 2529
Fragments arranged by size:
2529 1723 32 30 30 2
HincII GTy'rAC
Cuts at: 1385 1385
Size: 4346
HindIII A'AGCT_T
Cuts at: 629 629
Size: 4346
KasI G'GCGC_C
Cuts at: 1776 2024 1776
Size: 248 4098
KpnI G_GTAC'C
Cuts at: 1366 1366
Size: 4346
MfeI C'AATT_G
Cuts at: 437 437
Size: 4346
MluI A'CGCG_T
Cuts at: 3779 3779
Size: 4346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 627 3017 3201 627
Size: 2390 184 1772
Fragments arranged by size:
2390 1772 184
MscI TGG'CCA
Cuts at: 532 2107 532
Size: 1575 2771
MslI CAynn'nnrTG
Cuts at: 718 1012 2335 2462 718
Size: 294 1323 127 2602
Fragments arranged by size:
2602 1323 294 127
MspA1I CmG'CkG
Cuts at: 828 899 1201 1457 1771 2131 3144 3389 828
Size: 71 302 256 314 360 1013 245 1785
Fragments arranged by size:
1785 1013 360 314 302 256 245 71
NaeI GCC'GGC
Cuts at: 2527 2527
Size: 4346
NarI GG'CG_CC
Cuts at: 1777 2025 1777
Size: 248 4098
NcoI C'CATG_G
Cuts at: 107 312 815 107
Size: 205 503 3638
Fragments arranged by size:
3638 503 205
NgoMIV G'CCGG_C
Cuts at: 2525 2525
Size: 4346
NsiI A_TGCA'T
Cuts at: 192 192
Size: 4346
NspI r_CATG'y
Cuts at: 1017 1399 1899 2806 1017
Size: 382 500 907 2557
Fragments arranged by size:
2557 907 500 382
PasI CC'CwG_GG
Cuts at: 1080 1080
Size: 4346
PciI A'CATG_T
Cuts at: 2802 2802
Size: 4346
PflMI CCAn_nnn'nTGG
Cuts at: 766 766
Size: 4346
PfoI T'CCnGG_A
Cuts at: 397 397
Size: 4346
PmeI GTTT'AAAC
Cuts at: 1345 1345
Size: 4346
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3485 3517 3485
Size: 32 4314
PsiI TTA'TAA
Cuts at: 290 1276 290
Size: 986 3360
PstI C_TGCA'G
Cuts at: 827 1393 2078 827
Size: 566 685 3095
Fragments arranged by size:
3095 685 566
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4346
PvuII CAG'CTG
Cuts at: 828 899 1201 1771 2131 828
Size: 71 302 570 360 3043
Fragments arranged by size:
3043 570 360 302 71
RsrII CG'GwC_CG
Cuts at: 2541 2541
Size: 4346
SacI G_AGCT'C
Cuts at: 1360 1360
Size: 4346
SalI G'TCGA_C
Cuts at: 1383 1383
Size: 4346
SapI GCTCTTCn'nnn_
Cuts at: 2369 2579 2369
Size: 210 4136
SbfI CC_TGCA'GG
Cuts at: 1393 1393
Size: 4346
ScaI AGT'ACT
Cuts at: 3863 3863
Size: 4346
SfcI C'TryA_G
Cuts at: 33 823 1196 1389 2074 3067 3258 33
Size: 790 373 193 685 993 191 1121
Fragments arranged by size:
1121 993 790 685 373 193 191
SfoI GGC'GCC
Cuts at: 1778 2026 1778
Size: 248 4098
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4346
SmaI CCC'GGG
Cuts at: 1368 1368
Size: 4346
SmlI C'TyrA_G
Cuts at: 1501 2416 2908 3170 1501
Size: 915 492 262 2677
Fragments arranged by size:
2677 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4346
SphI G_CATG'C
Cuts at: 1399 1899 1399
Size: 500 3846
StyI C'CwwG_G
Cuts at: 107 312 416 815 1262 1480 1834 107
Size: 205 104 399 447 218 354 2619
Fragments arranged by size:
2619 447 399 354 218 205 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 717 1243 1929 2062 2521 2730 717
Size: 526 686 133 459 209 2333
Fragments arranged by size:
2333 686 526 459 209 133
TatI w'GTAC_w
Cuts at: 624 3861 624
Size: 3237 1109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 468 500 3863 3895 468
Size: 32 3363 32 919
Fragments arranged by size:
3363 919 32 32
Tth111I GACn'n_nGTC
Cuts at: 445 958 2143 445
Size: 513 1185 2648
Fragments arranged by size:
2648 1185 513
XbaI T'CTAG_A
Cuts at: 951 1377 951
Size: 426 3920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1841 1841
Size: 4346
XmaI C'CCGG_G
Cuts at: 1366 1366
Size: 4346
XmnI GAAnn'nnTTC
Cuts at: 774 1191 774
Size: 417 3929
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI BseRI BsiWI BsmI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI NdeI NheI NotI NruI PacI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI