Restriction map for
pF1KB9359
(Circular) (Six-base) MAPSORT of: pF1KB9359.seq Check: 2255 from: 1 to: 3989
pF1KB9359 3989 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1027 1027
Size: 3989
Acc65I G'GTAC_C
Cuts at: 1005 1005
Size: 3989
AclI AA'CG_TT
Cuts at: 3829 3829
Size: 3989
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 833 1814 2246 2599 3261 833
Size: 981 432 353 662 1561
Fragments arranged by size:
1561 981 662 432 353
AfeI AGC'GCT
Cuts at: 217 322 217
Size: 105 3884
AflIII A'CryG_T
Cuts at: 1341 3141 3422 1341
Size: 1800 281 1908
Fragments arranged by size:
1908 1800 281
AleI CACnn'nnGTG
Cuts at: 814 814
Size: 3989
AlwNI CAG_nnn'CTG
Cuts at: 198 411 1294 2732 198
Size: 213 883 1438 1455
Fragments arranged by size:
1455 1438 883 213
ApaI G_GGCC'C
Cuts at: 941 941
Size: 3989
ApaLI G'TGCA_C
Cuts at: 2827 2827
Size: 3989
ApoI r'AATT_y
Cuts at: 993 3342 3598 993
Size: 2349 256 1384
Fragments arranged by size:
2349 1384 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3989
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3989
AvaI C'yCGr_G
Cuts at: 146 917 1009 146
Size: 771 92 3126
Fragments arranged by size:
3126 771 92
AvrII C'CTAG_G
Cuts at: 540 540
Size: 3989
BamHI G'GATC_C
Cuts at: 1014 1014
Size: 3989
BanI G'GyrC_C
Cuts at: 130 754 1005 1419 1667 1702 130
Size: 624 251 414 248 35 2417
Fragments arranged by size:
2417 624 414 251 248 35
BanII G_rGCy'C
Cuts at: 782 925 941 1003 2033 782
Size: 143 16 62 1030 2738
Fragments arranged by size:
2738 1030 143 62 16
BbeI G_GCGC'C
Cuts at: 1423 1671 1423
Size: 248 3741
BbsI GAAGACnn'nnnn_
Cuts at: 330 330
Size: 3989
BciVI GTATCCnnnnn_n'
Cuts at: 1887 2943 3989 1887
Size: 1056 1046 1887
Fragments arranged by size:
1887 1056 1046
BclI T'GATC_A
Cuts at: 1509 1509
Size: 3989
BglII A'GATC_T
Cuts at: 1504 3541 1504
Size: 2037 1952
BlpI GC'TnA_GC
Cuts at: 1101 1101
Size: 3989
Bme1580I G_kGCm'C
Cuts at: 941 1614 1707 2831 941
Size: 673 93 1124 2099
Fragments arranged by size:
2099 1124 673 93
BmrI ACTGGGnnnn_n'
Cuts at: 534 1201 1282 1617 3761 534
Size: 667 81 335 2144 762
Fragments arranged by size:
2144 762 667 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2356 2356
Size: 3989
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 322 879 909 229
Size: 93 557 30 3309
Fragments arranged by size:
3309 557 93 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 1165 2044 2758 3056 535
Size: 630 879 714 298 1468
Fragments arranged by size:
1468 879 714 630 298
BsaI GGTCTCn'nnnn_
Cuts at: 445 3693 445
Size: 3248 741
BsaAI yAC'GTr
Cuts at: 222 1342 222
Size: 1120 2869
BsaBI GATnn'nnATC
Cuts at: 6 1503 3540 6
Size: 1497 2037 455
Fragments arranged by size:
2037 1497 455
BsaHI Gr'CG_yC
Cuts at: 1420 1668 2370 3866 1420
Size: 248 702 1496 1543
Fragments arranged by size:
1543 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1178 1699 2377 2788 2935 3209 1178
Size: 521 678 411 147 274 1958
Fragments arranged by size:
1958 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 103 133 939 969 103
Size: 30 806 30 3123
Fragments arranged by size:
3123 806 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 226 328 343 226
Size: 102 15 3872
Fragments arranged by size:
3872 102 15
BseYI C'CCAG_C
Cuts at: 171 900 940 1414 2172 2837 3245 171
Size: 729 40 474 758 665 408 915
Fragments arranged by size:
915 758 729 665 474 408 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 586 839 271
Size: 315 253 3421
Fragments arranged by size:
3421 315 253
BsiEI CG_ry'CG
Cuts at: 104 1531 1577 2807 104
Size: 1427 46 1230 1286
Fragments arranged by size:
1427 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 782 1003 1781 1971 2831 782
Size: 221 778 190 860 1940
Fragments arranged by size:
1940 860 778 221 190
Bsp1286I G_dGCh'C
Cuts at: 782 925 941 1003 1614 1707 1781 1971 2033
Size: 143 16 62 611 93 74 190 62
Cuts at: 2033 2831 782
Size: 798 1940
Fragments arranged by size:
1940 798 611 190 143 93 74 62
62 16
BspHI T'CATG_A
Cuts at: 2421 2421
Size: 3989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1039 1253 1555 1936 1039
Size: 214 302 381 3092
Fragments arranged by size:
3092 381 302 214
BsrBI CCG'CTC
Cuts at: 50 883 2281 2335 3819 3984 50
Size: 833 1398 54 1484 165 55
Fragments arranged by size:
1484 1398 833 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1901 1901
Size: 3989
BsrFI r'CCGG_y
Cuts at: 605 1987 2168 605
Size: 1382 181 2426
Fragments arranged by size:
2426 1382 181
BssSI C'ACGA_G
Cuts at: 2260 2968 2260
Size: 708 3281
BstAPI GCAn_nnn'nTGC
Cuts at: 198 1366 198
Size: 1168 2821
BstBI TT'CG_AA
Cuts at: 2350 2350
Size: 3989
BstXI CCAn_nnnn'nTGG
Cuts at: 981 981
Size: 3989
BstYI r'GATC_y
Cuts at: 1 1014 1481 1504 1839 2085 2391 2403 2489
Size: 1013 467 23 335 246 306 12 86
Cuts at: 2489 3541 1
Size: 1052 449
Fragments arranged by size:
1052 1013 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 871 871
Size: 3989
BtgI C'CryG_G
Cuts at: 107 163 719 107
Size: 56 556 3377
Fragments arranged by size:
3377 556 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1939 2120 2283 1939
Size: 181 163 3645
Fragments arranged by size:
3645 181 163
BtsI GCAGTG_nn'
Cuts at: 746 1232 746
Size: 486 3503
DraI TTT'AAA
Cuts at: 988 988
Size: 3989
DrdI GACnn_nn'nnGTC
Cuts at: 184 499 1695 3039 184
Size: 315 1196 1344 1134
Fragments arranged by size:
1344 1196 1134 315
EaeI y'GGCC_r
Cuts at: 347 1574 1748 2139 2166 3511 347
Size: 1227 174 391 27 1345 825
Fragments arranged by size:
1345 1227 825 391 174 27
EagI C'GGCC_G
Cuts at: 1574 1574
Size: 3989
EarI CTCTTCn'nnn_
Cuts at: 2012 2222 2012
Size: 210 3779
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 193 2197 2939 3085 3799 193
Size: 2004 742 146 714 383
Fragments arranged by size:
2004 742 714 383 146
EcoICRI GAG'CTC
Cuts at: 780 1001 780
Size: 221 3768
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 322 833 879 909 1814 2246 2599 3261
Size: 93 511 46 30 905 432 353 662
Cuts at: 3261 229
Size: 957
Fragments arranged by size:
957 905 662 511 432 353 93 46
30
EcoO109I rG'GnC_Cy
Cuts at: 558 937 1128 558
Size: 379 191 3419
Fragments arranged by size:
3419 379 191
EcoRI G'AATT_C
Cuts at: 993 993
Size: 3989
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2008 2008
Size: 3989
FspI TGC'GCA
Cuts at: 1770 1770
Size: 3989
HaeII r_GCGC'y
Cuts at: 219 324 907 1423 1671 2901 3672 219
Size: 105 583 516 248 1230 771 536
Fragments arranged by size:
1230 771 583 536 516 248 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 939 971 1831 1861 1863 1893 939
Size: 32 860 30 2 30 3035
Fragments arranged by size:
3035 860 32 30 30 2
HincII GTy'rAC
Cuts at: 1028 1028
Size: 3989
KasI G'GCGC_C
Cuts at: 1419 1667 1419
Size: 248 3741
KpnI G_GTAC'C
Cuts at: 1009 1009
Size: 3989
MluI A'CGCG_T
Cuts at: 3422 3422
Size: 3989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2748 2932 2748
Size: 184 3805
MscI TGG'CCA
Cuts at: 349 1750 349
Size: 1401 2588
MslI CAynn'nnrTG
Cuts at: 814 1978 2105 814
Size: 1164 127 2698
Fragments arranged by size:
2698 1164 127
MspA1I CmG'CkG
Cuts at: 165 255 1100 1414 1774 2558 2803 165
Size: 90 845 314 360 784 245 1351
Fragments arranged by size:
1351 845 784 360 314 245 90
NaeI GCC'GGC
Cuts at: 2170 2170
Size: 3989
NarI GG'CG_CC
Cuts at: 1420 1668 1420
Size: 248 3741
NcoI C'CATG_G
Cuts at: 107 107
Size: 3989
NgoMIV G'CCGG_C
Cuts at: 2168 2168
Size: 3989
NspI r_CATG'y
Cuts at: 276 429 652 1042 1542 3145 276
Size: 153 223 390 500 1603 1120
Fragments arranged by size:
1603 1120 500 390 223 153
PciI A'CATG_T
Cuts at: 3141 3141
Size: 3989
PmeI GTTT'AAAC
Cuts at: 988 988
Size: 3989
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2435 2467 2435
Size: 32 3957
PpuMI rG'GwC_Cy
Cuts at: 558 558
Size: 3989
PspOMI G'GGCC_C
Cuts at: 937 937
Size: 3989
PstI C_TGCA'G
Cuts at: 194 741 1036 1721 194
Size: 547 295 685 2462
Fragments arranged by size:
2462 685 547 295
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3989
PvuII CAG'CTG
Cuts at: 1414 1774 1414
Size: 360 3629
RsrII CG'GwC_CG
Cuts at: 2184 2184
Size: 3989
SacI G_AGCT'C
Cuts at: 782 1003 782
Size: 221 3768
SacII CC_GC'GG
Cuts at: 166 166
Size: 3989
SalI G'TCGA_C
Cuts at: 1026 1026
Size: 3989
SapI GCTCTTCn'nnn_
Cuts at: 2012 2222 2012
Size: 210 3779
SbfI CC_TGCA'GG
Cuts at: 1036 1036
Size: 3989
ScaI AGT'ACT
Cuts at: 3506 3506
Size: 3989
SexAI A'CCwGG_T
Cuts at: 980 980
Size: 3989
SfcI C'TryA_G
Cuts at: 33 190 677 737 959 1032 1717 2685 2876
Size: 157 487 60 222 73 685 968 191
Cuts at: 2876 33
Size: 1146
Fragments arranged by size:
1146 968 685 487 222 191 157 73
60
SfoI GGC'GCC
Cuts at: 1421 1669 1421
Size: 248 3741
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3989
SmaI CCC'GGG
Cuts at: 148 919 1011 148
Size: 771 92 3126
Fragments arranged by size:
3126 771 92
SmlI C'TyrA_G
Cuts at: 550 1144 2059 2773 3035 550
Size: 594 915 714 262 1504
Fragments arranged by size:
1504 915 714 594 262
SnaBI TAC'GTA
Cuts at: 222 222
Size: 3989
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3989
SphI G_CATG'C
Cuts at: 429 652 1042 1542 429
Size: 223 390 500 2876
Fragments arranged by size:
2876 500 390 223
StuI AGG'CCT
Cuts at: 634 749 634
Size: 115 3874
StyI C'CwwG_G
Cuts at: 107 540 635 933 1123 1477 107
Size: 433 95 298 190 354 2619
Fragments arranged by size:
2619 433 354 298 190 95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 403 641 742 1572 1705 2164 2373 403
Size: 238 101 830 133 459 209 2019
Fragments arranged by size:
2019 830 459 238 209 133 101
TatI w'GTAC_w
Cuts at: 3504 3504
Size: 3989
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 103 135 569 601 3506 3538 103
Size: 32 434 32 2905 32 554
Fragments arranged by size:
2905 554 434 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1786 1786
Size: 3989
XbaI T'CTAG_A
Cuts at: 1020 1020
Size: 3989
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 357 1484 357
Size: 1127 2862
XmaI C'CCGG_G
Cuts at: 146 917 1009 146
Size: 771 92 3126
Fragments arranged by size:
3126 771 92
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AlfI AloI AscI
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspXI PsrI
SanDI SfiI SgrAI SrfI SspI SwaI XhoI XmnI
ZraI