Restriction map for
pF1KB9348
(Circular) (Six-base) MAPSORT of: pF1KB9348.seq Check: 6171 from: 1 to: 3932
pF1KB9348 3932 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 970 970
Size: 3932
Acc65I G'GTAC_C
Cuts at: 948 948
Size: 3932
AclI AA'CG_TT
Cuts at: 3772 3772
Size: 3932
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 1757 2189 2542 3204 676
Size: 1081 432 353 662 1404
Fragments arranged by size:
1404 1081 662 432 353
AflIII A'CryG_T
Cuts at: 1284 3084 3365 1284
Size: 1800 281 1851
Fragments arranged by size:
1851 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 524 524
Size: 3932
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 656 656
Size: 3932
AlwNI CAG_nnn'CTG
Cuts at: 1237 2675 1237
Size: 1438 2494
ApaI G_GGCC'C
Cuts at: 923 923
Size: 3932
ApaLI G'TGCA_C
Cuts at: 2770 2770
Size: 3932
ApoI r'AATT_y
Cuts at: 936 3285 3541 936
Size: 2349 256 1327
Fragments arranged by size:
2349 1327 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3932
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3932
AvaI C'yCGr_G
Cuts at: 952 952
Size: 3932
BamHI G'GATC_C
Cuts at: 957 957
Size: 3932
BanI G'GyrC_C
Cuts at: 779 948 1362 1610 1645 779
Size: 169 414 248 35 3066
Fragments arranged by size:
3066 414 248 169 35
BanII G_rGCy'C
Cuts at: 617 923 946 1976 617
Size: 306 23 1030 2573
Fragments arranged by size:
2573 1030 306 23
BbeI G_GCGC'C
Cuts at: 783 1366 1614 783
Size: 583 248 3101
Fragments arranged by size:
3101 583 248
BbvCI CC'TCA_GC
Cuts at: 644 644
Size: 3932
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 873 907 873
Size: 34 3898
BciVI GTATCCnnnnn_n'
Cuts at: 1830 2886 3932 1830
Size: 1056 1046 1830
Fragments arranged by size:
1830 1056 1046
BclI T'GATC_A
Cuts at: 671 1452 671
Size: 781 3151
BglII A'GATC_T
Cuts at: 1447 3484 1447
Size: 2037 1895
BlpI GC'TnA_GC
Cuts at: 1044 1044
Size: 3932
Bme1580I G_kGCm'C
Cuts at: 923 1557 1650 2774 923
Size: 634 93 1124 2081
Fragments arranged by size:
2081 1124 634 93
BmrI ACTGGGnnnn_n'
Cuts at: 518 1144 1225 1560 3704 518
Size: 626 81 335 2144 746
Fragments arranged by size:
2144 746 626 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 536 2299 536
Size: 1763 2169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 220 325 355 523 579 841 160
Size: 60 105 30 168 56 262 3251
Fragments arranged by size:
3251 262 168 105 60 56 30
Bpu10I CC'TnA_GC
Cuts at: 644 644
Size: 3932
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 112 1108 1987 2701 2999 112
Size: 996 879 714 298 1045
Fragments arranged by size:
1045 996 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 901 3636 901
Size: 2735 1197
BsaAI yAC'GTr
Cuts at: 1285 1285
Size: 3932
BsaBI GATnn'nnATC
Cuts at: 6 802 1446 3483 6
Size: 796 644 2037 455
Fragments arranged by size:
2037 796 644 455
BsaHI Gr'CG_yC
Cuts at: 780 1363 1611 2313 3809 780
Size: 583 248 702 1496 903
Fragments arranged by size:
1496 903 702 583 248
BsaWI w'CCGG_w
Cuts at: 570 1121 1642 2320 2731 2878 3152 570
Size: 551 521 678 411 147 274 1350
Fragments arranged by size:
1350 678 551 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 217 438 661 217
Size: 221 223 3488
Fragments arranged by size:
3488 223 221
BseYI C'CCAG_C
Cuts at: 662 1357 2115 2780 3188 662
Size: 695 758 665 408 1406
Fragments arranged by size:
1406 758 695 665 408
BsiEI CG_ry'CG
Cuts at: 104 1474 1520 2750 104
Size: 1370 46 1230 1286
Fragments arranged by size:
1370 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 146 593 617 946 1724 1914 2774 146
Size: 447 24 329 778 190 860 1304
Fragments arranged by size:
1304 860 778 447 329 190 24
Bsp1286I G_dGCh'C
Cuts at: 146 593 617 923 946 1557 1650 1724 1914
Size: 447 24 306 23 611 93 74 190
Cuts at: 1914 1976 2774 146
Size: 62 798 1304
Fragments arranged by size:
1304 798 611 447 306 190 93 74
62 24 23
BspEI T'CCGG_A
Cuts at: 570 570
Size: 3932
BspHI T'CATG_A
Cuts at: 2364 2364
Size: 3932
BspMI ACCTGCnnnn'nnnn_
Cuts at: 386 982 1196 1498 1879 386
Size: 596 214 302 381 2439
Fragments arranged by size:
2439 596 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2224 2278 3762 3927 50
Size: 2174 54 1484 165 55
Fragments arranged by size:
2174 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1844 1844
Size: 3932
BsrFI r'CCGG_y
Cuts at: 1930 2111 1930
Size: 181 3751
BssSI C'ACGA_G
Cuts at: 2203 2911 2203
Size: 708 3224
BstAPI GCAn_nnn'nTGC
Cuts at: 150 1309 150
Size: 1159 2773
BstBI TT'CG_AA
Cuts at: 2293 2293
Size: 3932
BstXI CCAn_nnnn'nTGG
Cuts at: 231 263 366 546 231
Size: 32 103 180 3617
Fragments arranged by size:
3617 180 103 32
BstYI r'GATC_y
Cuts at: 1 957 1424 1447 1782 2028 2334 2346 2432
Size: 956 467 23 335 246 306 12 86
Cuts at: 2432 3484 1
Size: 1052 449
Fragments arranged by size:
1052 956 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 119 119
Size: 3932
BtgI C'CryG_G
Cuts at: 107 230 107
Size: 123 3809
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1882 2063 2226 1882
Size: 181 163 3588
Fragments arranged by size:
3588 181 163
BtsI GCAGTG_nn'
Cuts at: 452 1175 452
Size: 723 3209
DraI TTT'AAA
Cuts at: 931 931
Size: 3932
DrdI GACnn_nn'nnGTC
Cuts at: 894 1638 2982 894
Size: 744 1344 1844
Fragments arranged by size:
1844 1344 744
EaeI y'GGCC_r
Cuts at: 157 854 1517 1691 2082 2109 3454 157
Size: 697 663 174 391 27 1345 635
Fragments arranged by size:
1345 697 663 635 391 174 27
EagI C'GGCC_G
Cuts at: 1517 1517
Size: 3932
EarI CTCTTCn'nnn_
Cuts at: 160 429 622 1955 2165 160
Size: 269 193 1333 210 1927
Fragments arranged by size:
1927 1333 269 210 193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2140 2882 3028 3742 2140
Size: 742 146 714 2330
Fragments arranged by size:
2330 742 714 146
EcoICRI GAG'CTC
Cuts at: 615 944 615
Size: 329 3603
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 220 325 355 523 579 676 841 1757
Size: 60 105 30 168 56 97 165 916
Cuts at: 1757 2189 2542 3204 160
Size: 432 353 662 888
Fragments arranged by size:
916 888 662 432 353 168 165 105
97 60 56 30
EcoNI CCTnn'n_nnAGG
Cuts at: 505 823 505
Size: 318 3614
EcoO109I rG'GnC_Cy
Cuts at: 123 651 920 1071 123
Size: 528 269 151 2984
Fragments arranged by size:
2984 528 269 151
EcoRI G'AATT_C
Cuts at: 936 936
Size: 3932
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 708 1951 708
Size: 1243 2689
FspI TGC'GCA
Cuts at: 1713 1713
Size: 3932
HaeII r_GCGC'y
Cuts at: 783 1366 1614 2844 3615 783
Size: 583 248 1230 771 1100
Fragments arranged by size:
1230 1100 771 583 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 277 309 510 542 1774 1804 1806 1836 277
Size: 32 201 32 1232 30 2 30 2373
Fragments arranged by size:
2373 1232 201 32 32 30 30 2
HincII GTy'rAC
Cuts at: 971 971
Size: 3932
KasI G'GCGC_C
Cuts at: 779 1362 1610 779
Size: 583 248 3101
Fragments arranged by size:
3101 583 248
KpnI G_GTAC'C
Cuts at: 952 952
Size: 3932
MfeI C'AATT_G
Cuts at: 540 540
Size: 3932
MluI A'CGCG_T
Cuts at: 3365 3365
Size: 3932
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2691 2875 2691
Size: 184 3748
MscI TGG'CCA
Cuts at: 159 1693 159
Size: 1534 2398
MslI CAynn'nnrTG
Cuts at: 112 1921 2048 112
Size: 1809 127 1996
Fragments arranged by size:
1996 1809 127
MspA1I CmG'CkG
Cuts at: 1043 1357 1717 2501 2746 1043
Size: 314 360 784 245 2229
Fragments arranged by size:
2229 784 360 314 245
NaeI GCC'GGC
Cuts at: 2113 2113
Size: 3932
NarI GG'CG_CC
Cuts at: 780 1363 1611 780
Size: 583 248 3101
Fragments arranged by size:
3101 583 248
NcoI C'CATG_G
Cuts at: 107 230 107
Size: 123 3809
NdeI CA'TA_TG
Cuts at: 282 282
Size: 3932
NgoMIV G'CCGG_C
Cuts at: 2111 2111
Size: 3932
NspI r_CATG'y
Cuts at: 117 985 1485 3088 117
Size: 868 500 1603 961
Fragments arranged by size:
1603 961 868 500
PciI A'CATG_T
Cuts at: 3084 3084
Size: 3932
PflMI CCAn_nnn'nTGG
Cuts at: 286 286
Size: 3932
PfoI T'CCnGG_A
Cuts at: 818 818
Size: 3932
PmeI GTTT'AAAC
Cuts at: 931 931
Size: 3932
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2378 2410 2378
Size: 32 3900
PpuMI rG'GwC_Cy
Cuts at: 123 651 123
Size: 528 3404
PspOMI G'GGCC_C
Cuts at: 919 919
Size: 3932
PstI C_TGCA'G
Cuts at: 979 1664 979
Size: 685 3247
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3932
PvuII CAG'CTG
Cuts at: 1357 1717 1357
Size: 360 3572
RsrII CG'GwC_CG
Cuts at: 2127 2127
Size: 3932
SacI G_AGCT'C
Cuts at: 617 946 617
Size: 329 3603
SalI G'TCGA_C
Cuts at: 969 969
Size: 3932
SapI GCTCTTCn'nnn_
Cuts at: 429 622 1955 2165 429
Size: 193 1333 210 2196
Fragments arranged by size:
2196 1333 210 193
SbfI CC_TGCA'GG
Cuts at: 979 979
Size: 3932
ScaI AGT'ACT
Cuts at: 724 3449 724
Size: 2725 1207
SexAI A'CCwGG_T
Cuts at: 584 584
Size: 3932
SfcI C'TryA_G
Cuts at: 33 389 975 1660 2628 2819 33
Size: 356 586 685 968 191 1146
Fragments arranged by size:
1146 968 685 586 356 191
SfoI GGC'GCC
Cuts at: 781 1364 1612 781
Size: 583 248 3101
Fragments arranged by size:
3101 583 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3932
SmaI CCC'GGG
Cuts at: 954 954
Size: 3932
SmlI C'TyrA_G
Cuts at: 127 1087 2002 2716 2978 127
Size: 960 915 714 262 1081
Fragments arranged by size:
1081 960 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3932
SphI G_CATG'C
Cuts at: 985 1485 985
Size: 500 3432
StyI C'CwwG_G
Cuts at: 107 154 230 923 1066 1420 107
Size: 47 76 693 143 354 2619
Fragments arranged by size:
2619 693 354 143 76 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 273 376 1515 1648 2107 2316 273
Size: 103 1139 133 459 209 1889
Fragments arranged by size:
1889 1139 459 209 133 103
TatI w'GTAC_w
Cuts at: 308 722 3447 308
Size: 414 2725 793
Fragments arranged by size:
2725 793 414
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3449 3481 3449
Size: 32 3900
Tth111I GACn'n_nGTC
Cuts at: 1729 1729
Size: 3932
XbaI T'CTAG_A
Cuts at: 963 963
Size: 3932
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1427 1427
Size: 3932
XmaI C'CCGG_G
Cuts at: 952 952
Size: 3932
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AloI AscI
AvrII BaeI BbsI BfrBI BglI BmgBI BmtI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI