Restriction map for
pF1KB9340
(Circular) (Six-base) MAPSORT of: pF1KB9340.seq Check: 9508 from: 1 to: 5411
pF1KB9340 5411 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AatII G_ACGT'C
Cuts at: 2156 2156
Size: 5411
AccI GT'mk_AC
Cuts at: 2449 2449
Size: 5411
Acc65I G'GTAC_C
Cuts at: 2427 2427
Size: 5411
AclI AA'CG_TT
Cuts at: 5251 5251
Size: 5411
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 958 1403 1680 2041 2308 3236 3668 4021 4683
Size: 445 277 361 267 928 432 353 662
Cuts at: 4683 958
Size: 1686
Fragments arranged by size:
1686 928 662 445 432 361 353 277
267
AflII C'TTAA_G
Cuts at: 994 1183 994
Size: 189 5222
AflIII A'CryG_T
Cuts at: 1454 2132 2763 4563 4844 1454
Size: 678 631 1800 281 2021
Fragments arranged by size:
2021 1800 678 631 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2282 2282
Size: 5411
AlwNI CAG_nnn'CTG
Cuts at: 523 2716 4154 523
Size: 2193 1438 1780
Fragments arranged by size:
2193 1780 1438
ApaLI G'TGCA_C
Cuts at: 359 2257 4249 359
Size: 1898 1992 1521
Fragments arranged by size:
1992 1898 1521
ApoI r'AATT_y
Cuts at: 904 1094 1477 1498 2000 2415 4764 5020 904
Size: 190 383 21 502 415 2349 256 1295
Fragments arranged by size:
2349 1295 502 415 383 256 190 21
AseI AT'TA_AT
Cuts at: 20 20
Size: 5411
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5411
AvaI C'yCGr_G
Cuts at: 2431 2431
Size: 5411
BamHI G'GATC_C
Cuts at: 1775 2436 1775
Size: 661 4750
BanI G'GyrC_C
Cuts at: 984 2140 2427 2841 3089 3124 984
Size: 1156 287 414 248 35 3271
Fragments arranged by size:
3271 1156 414 287 248 35
BanII G_rGCy'C
Cuts at: 1767 2163 2425 3455 1767
Size: 396 262 1030 3723
Fragments arranged by size:
3723 1030 396 262
BbeI G_GCGC'C
Cuts at: 2845 3093 2845
Size: 248 5163
BbsI GAAGACnn'nnnn_
Cuts at: 1355 1355
Size: 5411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1834 1868 1834
Size: 34 5377
BciVI GTATCCnnnnn_n'
Cuts at: 3309 4365 5411 3309
Size: 1056 1046 3309
Fragments arranged by size:
3309 1056 1046
BclI T'GATC_A
Cuts at: 2373 2931 2373
Size: 558 4853
BglI GCCn_nnn'nGGC
Cuts at: 327 327
Size: 5411
BglII A'GATC_T
Cuts at: 171 2926 4963 171
Size: 2755 2037 619
Fragments arranged by size:
2755 2037 619
BlpI GC'TnA_GC
Cuts at: 1748 2523 1748
Size: 775 4636
Bme1580I G_kGCm'C
Cuts at: 363 1475 2145 2261 3036 3129 4253 363
Size: 1112 670 116 775 93 1124 1521
Fragments arranged by size:
1521 1124 1112 775 670 116 93
BmrI ACTGGGnnnn_n'
Cuts at: 152 589 1065 2238 2623 2704 3039 5183 152
Size: 437 476 1173 385 81 335 2144 380
Fragments arranged by size:
2144 1173 476 437 385 380 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3778 3778
Size: 5411
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 697 943 566
Size: 131 246 5034
Fragments arranged by size:
5034 246 131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2587 3466 4180 4478 2587
Size: 879 714 298 3520
Fragments arranged by size:
3520 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5115 5115
Size: 5411
BsaAI yAC'GTr
Cuts at: 2764 2764
Size: 5411
BsaBI GATnn'nnATC
Cuts at: 6 2375 2925 4962 6
Size: 2369 550 2037 455
Fragments arranged by size:
2369 2037 550 455
BsaHI Gr'CG_yC
Cuts at: 2153 2842 3090 3792 5288 2153
Size: 689 248 702 1496 2276
Fragments arranged by size:
2276 1496 702 689 248
BsaWI w'CCGG_w
Cuts at: 2600 3121 3799 4210 4357 4631 2600
Size: 521 678 411 147 274 3380
Fragments arranged by size:
3380 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 287 317 998 1028 287
Size: 30 681 30 4670
Fragments arranged by size:
4670 681 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 295 430 433 295
Size: 135 3 5273
Fragments arranged by size:
5273 135 3
BseYI C'CCAG_C
Cuts at: 311 2836 3594 4259 4667 311
Size: 2525 758 665 408 1055
Fragments arranged by size:
2525 1055 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2067 2067
Size: 5411
BsiEI CG_ry'CG
Cuts at: 104 2953 2999 4229 104
Size: 2849 46 1230 1286
Fragments arranged by size:
2849 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 363 602 2261 2425 3203 3393 4253 363
Size: 239 1659 164 778 190 860 1521
Fragments arranged by size:
1659 1521 860 778 239 190 164
BsmI GAATG_Cn'
Cuts at: 938 2407 938
Size: 1469 3942
Bsp1286I G_dGCh'C
Cuts at: 363 602 1475 1767 2145 2163 2261 2425 3036
Size: 239 873 292 378 18 98 164 611
Cuts at: 3036 3129 3203 3393 3455 4253 363
Size: 93 74 190 62 798 1521
Fragments arranged by size:
1521 873 798 611 378 292 239 190
164 98 93 74 62 18
BspHI T'CATG_A
Cuts at: 3843 3843
Size: 5411
BspMI ACCTGCnnnn'nnnn_
Cuts at: 132 2228 2461 2675 2977 3358 132
Size: 2096 233 214 302 381 2185
Fragments arranged by size:
2185 2096 381 302 233 214
BsrBI CCG'CTC
Cuts at: 50 3703 3757 5241 5406 50
Size: 3653 54 1484 165 55
Fragments arranged by size:
3653 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1040 3323 1040
Size: 2283 3128
BsrFI r'CCGG_y
Cuts at: 3409 3590 3409
Size: 181 5230
BsrGI T'GTAC_A
Cuts at: 2129 2129
Size: 5411
BssSI C'ACGA_G
Cuts at: 3682 4390 3682
Size: 708 4703
BstAPI GCAn_nnn'nTGC
Cuts at: 1140 2788 1140
Size: 1648 3763
BstBI TT'CG_AA
Cuts at: 1509 3772 1509
Size: 2263 3148
BstXI CCAn_nnnn'nTGG
Cuts at: 1585 1585
Size: 5411
BstYI r'GATC_y
Cuts at: 1 171 1391 1775 2436 2903 2926 3261 3507
Size: 170 1220 384 661 467 23 335 246
Cuts at: 3507 3813 3825 3911 4963 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1220 1052 661 467 449 384 335 306
246 170 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5411
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3361 3542 3705 3361
Size: 181 163 5067
Fragments arranged by size:
5067 181 163
BtsI GCAGTG_nn'
Cuts at: 2654 2654
Size: 5411
ClaI AT'CG_AT
Cuts at: 1667 1667
Size: 5411
DraI TTT'AAA
Cuts at: 2410 2410
Size: 5411
DraIII CAC_nnn'GTG
Cuts at: 489 2341 489
Size: 1852 3559
DrdI GACnn_nn'nnGTC
Cuts at: 3117 4461 3117
Size: 1344 4067
EaeI y'GGCC_r
Cuts at: 374 2996 3170 3561 3588 4933 374
Size: 2622 174 391 27 1345 852
Fragments arranged by size:
2622 1345 852 391 174 27
EagI C'GGCC_G
Cuts at: 2996 2996
Size: 5411
EarI CTCTTCn'nnn_
Cuts at: 3434 3644 3434
Size: 210 5201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3619 4361 4507 5221 3619
Size: 742 146 714 3809
Fragments arranged by size:
3809 742 714 146
EcoICRI GAG'CTC
Cuts at: 2423 2423
Size: 5411
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 697 943 958 1403 1680 2041 2308 3236
Size: 131 246 15 445 277 361 267 928
Cuts at: 3236 3668 4021 4683 566
Size: 432 353 662 1294
Fragments arranged by size:
1294 928 662 445 432 361 353 277
267 246 131 15
EcoO109I rG'GnC_Cy
Cuts at: 1650 2550 1650
Size: 900 4511
EcoRI G'AATT_C
Cuts at: 2415 2415
Size: 5411
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1332 1921 2137 2329 3430 1332
Size: 589 216 192 1101 3313
Fragments arranged by size:
3313 1101 589 216 192
FspI TGC'GCA
Cuts at: 1625 3192 1625
Size: 1567 3844
HaeII r_GCGC'y
Cuts at: 2845 3093 4323 5094 2845
Size: 248 1230 771 3162
Fragments arranged by size:
3162 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 755 770 787 802 1379 1411 1714 1746 1999
Size: 15 17 15 577 32 303 32 253
Cuts at: 1999 2031 3253 3283 3285 3315 755
Size: 32 1222 30 2 30 2851
Fragments arranged by size:
2851 1222 577 303 253 32 32 32
30 30 17 15 15 2
HincII GTy'rAC
Cuts at: 774 2450 774
Size: 1676 3735
HindIII A'AGCT_T
Cuts at: 458 2090 458
Size: 1632 3779
KasI G'GCGC_C
Cuts at: 2841 3089 2841
Size: 248 5163
KpnI G_GTAC'C
Cuts at: 2431 2431
Size: 5411
MfeI C'AATT_G
Cuts at: 1706 1706
Size: 5411
MluI A'CGCG_T
Cuts at: 4844 4844
Size: 5411
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 797 1858 1913 4170 4354 797
Size: 1061 55 2257 184 1854
Fragments arranged by size:
2257 1854 1061 184 55
MscI TGG'CCA
Cuts at: 376 3172 376
Size: 2796 2615
MslI CAynn'nnrTG
Cuts at: 3400 3527 3400
Size: 127 5284
MspA1I CmG'CkG
Cuts at: 684 1158 2522 2836 3196 3980 4225 684
Size: 474 1364 314 360 784 245 1870
Fragments arranged by size:
1870 1364 784 474 360 314 245
NaeI GCC'GGC
Cuts at: 3592 3592
Size: 5411
NarI GG'CG_CC
Cuts at: 2842 3090 2842
Size: 248 5163
NcoI C'CATG_G
Cuts at: 107 107
Size: 5411
NgoMIV G'CCGG_C
Cuts at: 3590 3590
Size: 5411
NspI r_CATG'y
Cuts at: 1143 1458 1862 2136 2464 2964 4567 1143
Size: 315 404 274 328 500 1603 1987
Fragments arranged by size:
1987 1603 500 404 328 315 274
PciI A'CATG_T
Cuts at: 1454 2132 4563 1454
Size: 678 2431 2302
Fragments arranged by size:
2431 2302 678
PmeI GTTT'AAAC
Cuts at: 2410 2410
Size: 5411
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3857 3889 3857
Size: 32 5379
PstI C_TGCA'G
Cuts at: 1613 2274 2458 3143 1613
Size: 661 184 685 3881
Fragments arranged by size:
3881 685 661 184
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5411
PvuII CAG'CTG
Cuts at: 684 2836 3196 684
Size: 2152 360 2899
Fragments arranged by size:
2899 2152 360
RsrII CG'GwC_CG
Cuts at: 3606 3606
Size: 5411
SacI G_AGCT'C
Cuts at: 2425 2425
Size: 5411
SalI G'TCGA_C
Cuts at: 2448 2448
Size: 5411
SapI GCTCTTCn'nnn_
Cuts at: 3434 3644 3434
Size: 210 5201
SbfI CC_TGCA'GG
Cuts at: 2458 2458
Size: 5411
ScaI AGT'ACT
Cuts at: 4928 4928
Size: 5411
SexAI A'CCwGG_T
Cuts at: 2337 2337
Size: 5411
SfcI C'TryA_G
Cuts at: 33 573 1259 1609 2270 2454 3139 4107 4298
Size: 540 686 350 661 184 685 968 191
Cuts at: 4298 33
Size: 1146
Fragments arranged by size:
1146 968 686 685 661 540 350 191
184
SfoI GGC'GCC
Cuts at: 2843 3091 2843
Size: 248 5163
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5411
SmaI CCC'GGG
Cuts at: 2433 2433
Size: 5411
SmlI C'TyrA_G
Cuts at: 994 1183 2566 3481 4195 4457 994
Size: 189 1383 915 714 262 1948
Fragments arranged by size:
1948 1383 915 714 262 189
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5411
SphI G_CATG'C
Cuts at: 2464 2964 2464
Size: 500 4911
SspI AAT'ATT
Cuts at: 876 876
Size: 5411
StyI C'CwwG_G
Cuts at: 107 635 2080 2206 2366 2545 2899 107
Size: 528 1445 126 160 179 354 2619
Fragments arranged by size:
2619 1445 528 354 179 160 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 546 2994 3127 3586 3795 546
Size: 2448 133 459 209 2162
Fragments arranged by size:
2448 2162 459 209 133
TatI w'GTAC_w
Cuts at: 1463 2129 4926 1463
Size: 666 2797 1948
Fragments arranged by size:
2797 1948 666
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 998 1030 1559 1591 2024 2056 4928 4960 998
Size: 32 529 32 433 32 2872 32 1449
Fragments arranged by size:
2872 1449 529 433 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2244 3208 2244
Size: 964 4447
XbaI T'CTAG_A
Cuts at: 2442 2442
Size: 5411
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 384 1482 2178 2906 384
Size: 1098 696 728 2889
Fragments arranged by size:
2889 1098 728 696
XmaI C'CCGG_G
Cuts at: 2431 2431
Size: 5411
XmnI GAAnn'nnTTC
Cuts at: 558 1064 1094 1151 558
Size: 506 30 57 4818
Fragments arranged by size:
4818 506 57 30
ZraI GAC'GTC
Cuts at: 2154 2154
Size: 5411
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AgeI AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI
BsmBI BspEI BssHII BstEII BstZ17I Bsu36I CspCI EcoNI
EcoRV FseI FspAI HpaI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI