Restriction map for
pF1KB9290
(Circular) (Six-base) MAPSORT of: pF1KB9290.seq Check: 2751 from: 1 to: 4004
pF1KB9290 4004 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 1042 1042
Size: 4004
Acc65I G'GTAC_C
Cuts at: 1020 1020
Size: 4004
AclI AA'CG_TT
Cuts at: 587 3844 587
Size: 3257 747
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 329 805 1829 2261 3008 3276 256
Size: 73 476 1024 432 747 268 984
Fragments arranged by size:
1024 984 747 476 432 268 73
AflIII A'CryG_T
Cuts at: 1356 2460 3437 1356
Size: 1104 977 1923
Fragments arranged by size:
1923 1104 977
AgeI A'CCGG_T
Cuts at: 2392 2392
Size: 4004
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 224 256 224
Size: 32 3972
AlwNI CAG_nnn'CTG
Cuts at: 162 570 705 1309 2876 162
Size: 408 135 604 1567 1290
Fragments arranged by size:
1567 1290 604 408 135
ApaLI G'TGCA_C
Cuts at: 221 2774 221
Size: 2553 1451
ApoI r'AATT_y
Cuts at: 1008 3357 3613 1008
Size: 2349 256 1399
Fragments arranged by size:
2349 1399 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4004
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
AvaI C'yCGr_G
Cuts at: 548 1024 548
Size: 476 3528
BamHI G'GATC_C
Cuts at: 1029 1029
Size: 4004
BanI G'GyrC_C
Cuts at: 898 1020 1434 1682 1717 898
Size: 122 414 248 35 3185
Fragments arranged by size:
3185 414 248 122 35
BanII G_rGCy'C
Cuts at: 877 1018 2048 877
Size: 141 1030 2833
Fragments arranged by size:
2833 1030 141
BbeI G_GCGC'C
Cuts at: 1438 1686 1438
Size: 248 3756
BciVI GTATCCnnnnn_n'
Cuts at: 1902 2663 4004 1902
Size: 761 1341 1902
Fragments arranged by size:
1902 1341 761
BclI T'GATC_A
Cuts at: 1524 1524
Size: 4004
BglII A'GATC_T
Cuts at: 275 1519 3556 275
Size: 1244 2037 723
Fragments arranged by size:
2037 1244 723
BlpI GC'TnA_GC
Cuts at: 494 1116 494
Size: 622 3382
Bme1580I G_kGCm'C
Cuts at: 225 669 1629 1722 2778 225
Size: 444 960 93 1056 1451
Fragments arranged by size:
1451 1056 960 444 93
BmrI ACTGGGnnnn_n'
Cuts at: 318 668 1216 1297 1632 3776 318
Size: 350 548 81 335 2144 546
Fragments arranged by size:
2144 548 546 350 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2371 2371
Size: 4004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 292 302 372 652 973 229
Size: 63 10 70 280 321 3260
Fragments arranged by size:
3260 321 280 70 63 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1180 2059 2551 2849 1180
Size: 879 492 298 2335
Fragments arranged by size:
2335 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3708 3708
Size: 4004
BsaAI yAC'GTr
Cuts at: 670 1357 670
Size: 687 3317
BsaBI GATnn'nnATC
Cuts at: 6 1518 3555 6
Size: 1512 2037 455
Fragments arranged by size:
2037 1512 455
BsaHI Gr'CG_yC
Cuts at: 1435 1683 2385 3881 1435
Size: 248 702 1496 1558
Fragments arranged by size:
1558 1496 702 248
BsaWI w'CCGG_w
Cuts at: 519 1193 1714 2392 2666 2813 3224 519
Size: 674 521 678 274 147 411 1299
Fragments arranged by size:
1299 678 674 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 151 181 224 254 426 456 517 547 151
Size: 30 43 30 172 30 61 30 3608
Fragments arranged by size:
3608 172 61 43 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 232 232
Size: 4004
BseYI C'CCAG_C
Cuts at: 705 800 1429 2187 2764 3260 705
Size: 95 629 758 577 496 1449
Fragments arranged by size:
1449 758 629 577 496 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 271
Size: 4004
BsiEI CG_ry'CG
Cuts at: 104 837 1546 1592 2800 104
Size: 733 709 46 1208 1308
Fragments arranged by size:
1308 1208 733 709 46
BsiHKAI G_wGCw'C
Cuts at: 225 335 495 911 959 1018 1796 1986 2778
Size: 110 160 416 48 59 778 190 792
Cuts at: 2778 225
Size: 1451
Fragments arranged by size:
1451 792 778 416 190 160 110 59
48
Bsp1286I G_dGCh'C
Cuts at: 225 335 495 669 877 911 959 1018 1629
Size: 110 160 174 208 34 48 59 611
Cuts at: 1629 1722 1796 1986 2048 2778 225
Size: 93 74 190 62 730 1451
Fragments arranged by size:
1451 730 611 208 190 174 160 110
93 74 62 59 48 34
BspEI T'CCGG_A
Cuts at: 3224 3224
Size: 4004
BspHI T'CATG_A
Cuts at: 3180 3180
Size: 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 704 1054 1268 1570 1951 704
Size: 350 214 302 381 2757
Fragments arranged by size:
2757 381 350 302 214
BsrBI CCG'CTC
Cuts at: 50 839 2296 2350 3834 3999 50
Size: 789 1457 54 1484 165 55
Fragments arranged by size:
1484 1457 789 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1916 1916
Size: 4004
BsrFI r'CCGG_y
Cuts at: 776 832 2002 2183 2392 776
Size: 56 1170 181 209 2388
Fragments arranged by size:
2388 1170 209 181 56
BssSI C'ACGA_G
Cuts at: 2275 2633 2275
Size: 358 3646
BstAPI GCAn_nnn'nTGC
Cuts at: 1381 1381
Size: 4004
BstBI TT'CG_AA
Cuts at: 2365 2365
Size: 4004
BstXI CCAn_nnnn'nTGG
Cuts at: 114 951 114
Size: 837 3167
BstYI r'GATC_y
Cuts at: 1 275 1029 1496 1519 1854 2100 3112 3198
Size: 274 754 467 23 335 246 1012 86
Cuts at: 3198 3210 3556 1
Size: 12 346 449
Fragments arranged by size:
1012 754 467 449 346 335 274 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 966 966
Size: 4004
BtgI C'CryG_G
Cuts at: 812 812
Size: 4004
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1954 2135 2298 1954
Size: 181 163 3660
Fragments arranged by size:
3660 181 163
BtsI GCAGTG_nn'
Cuts at: 223 1247 223
Size: 1024 2980
DraI TTT'AAA
Cuts at: 1003 1003
Size: 4004
DrdI GACnn_nn'nnGTC
Cuts at: 1710 2568 1710
Size: 858 3146
EaeI y'GGCC_r
Cuts at: 834 1589 1763 2154 2181 3526 834
Size: 755 174 391 27 1345 1312
Fragments arranged by size:
1345 1312 755 391 174 27
EagI C'GGCC_G
Cuts at: 834 1589 834
Size: 755 3249
EarI CTCTTCn'nnn_
Cuts at: 2027 2237 2027
Size: 210 3794
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2212 2522 2668 3814 2212
Size: 310 146 1146 2402
Fragments arranged by size:
2402 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1016 1016
Size: 4004
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 256 292 302 329 372 652 805 973
Size: 27 36 10 27 43 280 153 168
Cuts at: 973 1829 2261 3008 3276 229
Size: 856 432 747 268 957
Fragments arranged by size:
957 856 747 432 280 268 168 153
43 36 27 27 10
EcoNI CCTnn'n_nnAGG
Cuts at: 154 296 465 868 154
Size: 142 169 403 3290
Fragments arranged by size:
3290 403 169 142
EcoO109I rG'GnC_Cy
Cuts at: 289 313 320 358 948 1143 289
Size: 24 7 38 590 195 3150
Fragments arranged by size:
3150 590 195 38 24 7
EcoRI G'AATT_C
Cuts at: 1008 1008
Size: 4004
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 765 2023 765
Size: 1258 2746
FspI TGC'GCA
Cuts at: 1785 1785
Size: 4004
HaeII r_GCGC'y
Cuts at: 1438 1686 2708 3687 1438
Size: 248 1022 979 1755
Fragments arranged by size:
1755 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 151 183 517 549 1846 1876 1878 1908 151
Size: 32 334 32 1297 30 2 30 2247
Fragments arranged by size:
2247 1297 334 32 32 30 30 2
HincII GTy'rAC
Cuts at: 394 1043 394
Size: 649 3355
KasI G'GCGC_C
Cuts at: 1434 1682 1434
Size: 248 3756
KpnI G_GTAC'C
Cuts at: 1024 1024
Size: 4004
MfeI C'AATT_G
Cuts at: 609 609
Size: 4004
MluI A'CGCG_T
Cuts at: 3437 3437
Size: 4004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2675 2859 2675
Size: 184 3820
MscI TGG'CCA
Cuts at: 1765 1765
Size: 4004
MslI CAynn'nnrTG
Cuts at: 112 434 541 1993 2120 112
Size: 322 107 1452 127 1996
Fragments arranged by size:
1996 1452 322 127 107
MspA1I CmG'CkG
Cuts at: 270 988 1115 1429 1789 2802 3047 270
Size: 718 127 314 360 1013 245 1227
Fragments arranged by size:
1227 1013 718 360 314 245 127
NaeI GCC'GGC
Cuts at: 2185 2185
Size: 4004
NarI GG'CG_CC
Cuts at: 1435 1683 1435
Size: 248 3756
NcoI C'CATG_G
Cuts at: 812 812
Size: 4004
NgoMIV G'CCGG_C
Cuts at: 2183 2183
Size: 4004
NspI r_CATG'y
Cuts at: 1057 1557 2464 1057
Size: 500 907 2597
Fragments arranged by size:
2597 907 500
PciI A'CATG_T
Cuts at: 2460 2460
Size: 4004
PflMI CCAn_nnn'nTGG
Cuts at: 705 705
Size: 4004
PmeI GTTT'AAAC
Cuts at: 1003 1003
Size: 4004
PmlI CAC'GTG
Cuts at: 670 670
Size: 4004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 224 256 3143 3175 224
Size: 32 2887 32 1053
Fragments arranged by size:
2887 1053 32 32
PpuMI rG'GwC_Cy
Cuts at: 289 313 320 358 289
Size: 24 7 38 3935
Fragments arranged by size:
3935 38 24 7
PstI C_TGCA'G
Cuts at: 1051 1736 1051
Size: 685 3319
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4004
PvuII CAG'CTG
Cuts at: 270 988 1429 1789 270
Size: 718 441 360 2485
Fragments arranged by size:
2485 718 441 360
RsrII CG'GwC_CG
Cuts at: 2199 2199
Size: 4004
SacI G_AGCT'C
Cuts at: 1018 1018
Size: 4004
SalI G'TCGA_C
Cuts at: 1041 1041
Size: 4004
SapI GCTCTTCn'nnn_
Cuts at: 2027 2237 2027
Size: 210 3794
SbfI CC_TGCA'GG
Cuts at: 1051 1051
Size: 4004
ScaI AGT'ACT
Cuts at: 3521 3521
Size: 4004
SexAI A'CCwGG_T
Cuts at: 599 599
Size: 4004
SfcI C'TryA_G
Cuts at: 33 1047 1732 2725 2916 33
Size: 1014 685 993 191 1121
Fragments arranged by size:
1121 1014 993 685 191
SfoI GGC'GCC
Cuts at: 1436 1684 1436
Size: 248 3756
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
SmaI CCC'GGG
Cuts at: 550 1026 550
Size: 476 3528
SmlI C'TyrA_G
Cuts at: 1159 2074 2566 2828 1159
Size: 915 492 262 2335
Fragments arranged by size:
2335 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4004
SphI G_CATG'C
Cuts at: 1057 1557 1057
Size: 500 3504
StyI C'CwwG_G
Cuts at: 812 869 944 1138 1492 812
Size: 57 75 194 354 3324
Fragments arranged by size:
3324 354 194 75 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 172 208 792 825 1587 1720 2179 2388 172
Size: 36 584 33 762 133 459 209 1788
Fragments arranged by size:
1788 762 584 459 209 133 36 33
TatI w'GTAC_w
Cuts at: 3519 3519
Size: 4004
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 405 426 437 458 800 832 3521 3553 405
Size: 21 11 21 342 32 2689 32 856
Fragments arranged by size:
2689 856 342 32 32 21 21 11
Tth111I GACn'n_nGTC
Cuts at: 1801 1801
Size: 4004
XbaI T'CTAG_A
Cuts at: 1035 1035
Size: 4004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 177 1499 177
Size: 1322 2682
XmaI C'CCGG_G
Cuts at: 548 1024 548
Size: 476 3528
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI