Restriction map for
pF1KB9284
(Circular) (Six-base) MAPSORT of: pF1KB9284.seq Check: 5657 from: 1 to: 3698
pF1KB9284 3698 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 736 736
Size: 3698
Acc65I G'GTAC_C
Cuts at: 583 714 583
Size: 131 3567
AclI AA'CG_TT
Cuts at: 3538 3538
Size: 3698
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1523 1955 2702 2970 1523
Size: 432 747 268 2251
Fragments arranged by size:
2251 747 432 268
AflIII A'CryG_T
Cuts at: 227 1050 2154 3131 227
Size: 823 1104 977 794
Fragments arranged by size:
1104 977 823 794
AgeI A'CCGG_T
Cuts at: 2086 2086
Size: 3698
AleI CACnn'nnGTG
Cuts at: 466 469 466
Size: 3 3695
AlwNI CAG_nnn'CTG
Cuts at: 211 429 1003 2570 211
Size: 218 574 1567 1339
Fragments arranged by size:
1567 1339 574 218
ApaI G_GGCC'C
Cuts at: 654 654
Size: 3698
ApaLI G'TGCA_C
Cuts at: 2468 2468
Size: 3698
ApoI r'AATT_y
Cuts at: 702 3051 3307 702
Size: 2349 256 1093
Fragments arranged by size:
2349 1093 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3698
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3698
AvaI C'yCGr_G
Cuts at: 718 718
Size: 3698
BamHI G'GATC_C
Cuts at: 723 723
Size: 3698
BanI G'GyrC_C
Cuts at: 583 714 1128 1376 1411 583
Size: 131 414 248 35 2870
Fragments arranged by size:
2870 414 248 131 35
BanII G_rGCy'C
Cuts at: 394 654 712 1742 394
Size: 260 58 1030 2350
Fragments arranged by size:
2350 1030 260 58
BbeI G_GCGC'C
Cuts at: 1132 1380 1132
Size: 248 3450
BciVI GTATCCnnnnn_n'
Cuts at: 242 1596 2357 3698 242
Size: 1354 761 1341 242
Fragments arranged by size:
1354 1341 761 242
BclI T'GATC_A
Cuts at: 1218 1218
Size: 3698
BglII A'GATC_T
Cuts at: 1213 3250 1213
Size: 2037 1661
BlpI GC'TnA_GC
Cuts at: 810 810
Size: 3698
Bme1580I G_kGCm'C
Cuts at: 641 654 683 1323 1416 2472 641
Size: 13 29 640 93 1056 1867
Fragments arranged by size:
1867 1056 640 93 29 13
BmgBI CAC'GTC
Cuts at: 450 450
Size: 3698
BmrI ACTGGGnnnn_n'
Cuts at: 910 991 1326 3470 910
Size: 81 335 2144 1138
Fragments arranged by size:
2144 1138 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2065 2065
Size: 3698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 257
Size: 3698
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 874 1753 2245 2543 874
Size: 879 492 298 2029
Fragments arranged by size:
2029 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3402 3402
Size: 3698
BsaAI yAC'GTr
Cuts at: 413 644 1051 413
Size: 231 407 3060
Fragments arranged by size:
3060 407 231
BsaBI GATnn'nnATC
Cuts at: 6 1212 3249 6
Size: 1206 2037 455
Fragments arranged by size:
2037 1206 455
BsaHI Gr'CG_yC
Cuts at: 1129 1377 2079 3575 1129
Size: 248 702 1496 1252
Fragments arranged by size:
1496 1252 702 248
BsaWI w'CCGG_w
Cuts at: 887 1408 2086 2360 2507 2918 887
Size: 521 678 274 147 411 1667
Fragments arranged by size:
1667 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 316 346 316
Size: 30 3668
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 318 321 434 318
Size: 3 113 3582
Fragments arranged by size:
3582 113 3
BseYI C'CCAG_C
Cuts at: 383 556 1123 1881 2458 2954 383
Size: 173 567 758 577 496 1127
Fragments arranged by size:
1127 758 577 567 496 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 493
Size: 3698
BsiEI CG_ry'CG
Cuts at: 104 1240 1286 2494 104
Size: 1136 46 1208 1308
Fragments arranged by size:
1308 1208 1136 46
BsiHKAI G_wGCw'C
Cuts at: 712 1490 1680 2472 712
Size: 778 190 792 1938
Fragments arranged by size:
1938 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 394 641 654 683 712 1323 1416 1490 1680
Size: 247 13 29 29 611 93 74 190
Cuts at: 1680 1742 2472 394
Size: 62 730 1620
Fragments arranged by size:
1620 730 611 247 190 93 74 62
29 29 13
BspEI T'CCGG_A
Cuts at: 2918 2918
Size: 3698
BspHI T'CATG_A
Cuts at: 2874 2874
Size: 3698
BspMI ACCTGCnnnn'nnnn_
Cuts at: 571 748 962 1264 1645 571
Size: 177 214 302 381 2624
Fragments arranged by size:
2624 381 302 214 177
BsrBI CCG'CTC
Cuts at: 50 1990 2044 3528 3693 50
Size: 1940 54 1484 165 55
Fragments arranged by size:
1940 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1610 1610
Size: 3698
BsrFI r'CCGG_y
Cuts at: 1696 1877 2086 1696
Size: 181 209 3308
Fragments arranged by size:
3308 209 181
BssSI C'ACGA_G
Cuts at: 1969 2327 1969
Size: 358 3340
BstAPI GCAn_nnn'nTGC
Cuts at: 211 1075 211
Size: 864 2834
BstBI TT'CG_AA
Cuts at: 2059 2059
Size: 3698
BstEII G'GTnAC_C
Cuts at: 537 537
Size: 3698
BstXI CCAn_nnnn'nTGG
Cuts at: 549 549
Size: 3698
BstYI r'GATC_y
Cuts at: 1 130 723 1190 1213 1548 1794 2806 2892
Size: 129 593 467 23 335 246 1012 86
Cuts at: 2892 2904 3250 1
Size: 12 346 449
Fragments arranged by size:
1012 593 467 449 346 335 246 129
86 23 12
BtgI C'CryG_G
Cuts at: 188 464 542 566 188
Size: 276 78 24 3320
Fragments arranged by size:
3320 276 78 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1648 1829 1992 1648
Size: 181 163 3354
Fragments arranged by size:
3354 181 163
BtsI GCAGTG_nn'
Cuts at: 941 941
Size: 3698
DraI TTT'AAA
Cuts at: 697 697
Size: 3698
DrdI GACnn_nn'nnGTC
Cuts at: 1404 2262 1404
Size: 858 2840
EaeI y'GGCC_r
Cuts at: 569 1283 1457 1848 1875 3220 569
Size: 714 174 391 27 1345 1047
Fragments arranged by size:
1345 1047 714 391 174 27
EagI C'GGCC_G
Cuts at: 1283 1283
Size: 3698
EarI CTCTTCn'nnn_
Cuts at: 1721 1931 1721
Size: 210 3488
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1906 2216 2362 3508 1906
Size: 310 146 1146 2096
Fragments arranged by size:
2096 1146 310 146
EcoICRI GAG'CTC
Cuts at: 710 710
Size: 3698
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 1523 1955 2702 2970 257
Size: 1266 432 747 268 985
Fragments arranged by size:
1266 985 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 524 524
Size: 3698
EcoO109I rG'GnC_Cy
Cuts at: 651 837 651
Size: 186 3512
EcoRI G'AATT_C
Cuts at: 702 702
Size: 3698
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1717 1717
Size: 3698
FspI TGC'GCA
Cuts at: 1479 1479
Size: 3698
HaeII r_GCGC'y
Cuts at: 1132 1380 2402 3381 1132
Size: 248 1022 979 1449
Fragments arranged by size:
1449 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1540 1570 1572 1602 1540
Size: 30 2 30 3636
Fragments arranged by size:
3636 30 30 2
HincII GTy'rAC
Cuts at: 737 737
Size: 3698
KasI G'GCGC_C
Cuts at: 1128 1376 1128
Size: 248 3450
KpnI G_GTAC'C
Cuts at: 587 718 587
Size: 131 3567
MluI A'CGCG_T
Cuts at: 3131 3131
Size: 3698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2369 2553 2369
Size: 184 3514
MscI TGG'CCA
Cuts at: 571 1459 571
Size: 888 2810
MslI CAynn'nnrTG
Cuts at: 466 469 547 571 1687 1814 466
Size: 3 78 24 1116 127 2350
Fragments arranged by size:
2350 1116 127 78 24 3
MspA1I CmG'CkG
Cuts at: 178 308 429 809 1123 1483 2496 2741 178
Size: 130 121 380 314 360 1013 245 1135
Fragments arranged by size:
1135 1013 380 360 314 245 130 121
NaeI GCC'GGC
Cuts at: 1879 1879
Size: 3698
NarI GG'CG_CC
Cuts at: 1129 1377 1129
Size: 248 3450
NcoI C'CATG_G
Cuts at: 188 542 566 188
Size: 354 24 3320
Fragments arranged by size:
3320 354 24
NgoMIV G'CCGG_C
Cuts at: 1877 1877
Size: 3698
NspI r_CATG'y
Cuts at: 231 489 751 1251 2158 231
Size: 258 262 500 907 1771
Fragments arranged by size:
1771 907 500 262 258
PciI A'CATG_T
Cuts at: 227 2154 227
Size: 1927 1771
PfoI T'CCnGG_A
Cuts at: 126 234 126
Size: 108 3590
PmeI GTTT'AAAC
Cuts at: 697 697
Size: 3698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2837 2869 2837
Size: 32 3666
PspOMI G'GGCC_C
Cuts at: 650 650
Size: 3698
PstI C_TGCA'G
Cuts at: 745 1430 745
Size: 685 3013
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3698
PvuII CAG'CTG
Cuts at: 178 1123 1483 178
Size: 945 360 2393
Fragments arranged by size:
2393 945 360
RsrII CG'GwC_CG
Cuts at: 1893 1893
Size: 3698
SacI G_AGCT'C
Cuts at: 712 712
Size: 3698
SalI G'TCGA_C
Cuts at: 735 735
Size: 3698
SapI GCTCTTCn'nnn_
Cuts at: 1721 1931 1721
Size: 210 3488
SbfI CC_TGCA'GG
Cuts at: 745 745
Size: 3698
ScaI AGT'ACT
Cuts at: 3215 3215
Size: 3698
SfcI C'TryA_G
Cuts at: 33 741 1426 2419 2610 33
Size: 708 685 993 191 1121
Fragments arranged by size:
1121 993 708 685 191
SfoI GGC'GCC
Cuts at: 1130 1378 1130
Size: 248 3450
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3698
SmaI CCC'GGG
Cuts at: 720 720
Size: 3698
SmlI C'TyrA_G
Cuts at: 853 1768 2260 2522 853
Size: 915 492 262 2029
Fragments arranged by size:
2029 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3698
SphI G_CATG'C
Cuts at: 489 751 1251 489
Size: 262 500 2936
Fragments arranged by size:
2936 500 262
StyI C'CwwG_G
Cuts at: 188 542 566 832 1186 188
Size: 354 24 266 354 2700
Fragments arranged by size:
2700 354 354 266 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1281 1414 1873 2082 1281
Size: 133 459 209 2897
Fragments arranged by size:
2897 459 209 133
TatI w'GTAC_w
Cuts at: 3213 3213
Size: 3698
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3215 3247 3215
Size: 32 3666
Tth111I GACn'n_nGTC
Cuts at: 1495 1495
Size: 3698
XbaI T'CTAG_A
Cuts at: 729 729
Size: 3698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 549 1193 549
Size: 644 3054
XmaI C'CCGG_G
Cuts at: 718 718
Size: 3698
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmtI
Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI