Restriction map for
pF1KB9279
(Circular) (Six-base) MAPSORT of: pF1KB9279.seq Check: 2294 from: 1 to: 4046
pF1KB9279 4046 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 1084 1084
Size: 4046
Acc65I G'GTAC_C
Cuts at: 1062 1062
Size: 4046
AclI AA'CG_TT
Cuts at: 3886 3886
Size: 4046
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 1871 2303 3050 3318 260
Size: 1611 432 747 268 988
Fragments arranged by size:
1611 988 747 432 268
AflIII A'CryG_T
Cuts at: 178 1398 2502 3479 178
Size: 1220 1104 977 745
Fragments arranged by size:
1220 1104 977 745
AgeI A'CCGG_T
Cuts at: 2434 2434
Size: 4046
AleI CACnn'nnGTG
Cuts at: 127 127
Size: 4046
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 155 155
Size: 4046
AlwNI CAG_nnn'CTG
Cuts at: 676 1351 2918 676
Size: 675 1567 1804
Fragments arranged by size:
1804 1567 675
ApaLI G'TGCA_C
Cuts at: 2816 2816
Size: 4046
ApoI r'AATT_y
Cuts at: 1050 3399 3655 1050
Size: 2349 256 1441
Fragments arranged by size:
2349 1441 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4046
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
AvaI C'yCGr_G
Cuts at: 1066 1066
Size: 4046
BamHI G'GATC_C
Cuts at: 1071 1071
Size: 4046
BanI G'GyrC_C
Cuts at: 1062 1476 1724 1759 1062
Size: 414 248 35 3349
Fragments arranged by size:
3349 414 248 35
BanII G_rGCy'C
Cuts at: 1060 2090 1060
Size: 1030 3016
BbeI G_GCGC'C
Cuts at: 1480 1728 1480
Size: 248 3798
BciVI GTATCCnnnnn_n'
Cuts at: 1944 2705 4046 1944
Size: 761 1341 1944
Fragments arranged by size:
1944 1341 761
BclI T'GATC_A
Cuts at: 1566 1566
Size: 4046
BglII A'GATC_T
Cuts at: 892 1561 3598 892
Size: 669 2037 1340
Fragments arranged by size:
2037 1340 669
BlpI GC'TnA_GC
Cuts at: 1158 1158
Size: 4046
Bme1580I G_kGCm'C
Cuts at: 1671 1764 2820 1671
Size: 93 1056 2897
Fragments arranged by size:
2897 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1258 1339 1674 3818 1258
Size: 81 335 2144 1486
Fragments arranged by size:
2144 1486 335 81
BmtI G_CTAG'C
Cuts at: 802 802
Size: 4046
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 284
Size: 4046
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 1222 2101 2593 2891 446
Size: 776 879 492 298 1601
Fragments arranged by size:
1601 879 776 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 947 3750 947
Size: 2803 1243
BsaAI yAC'GTr
Cuts at: 871 1399 871
Size: 528 3518
BsaBI GATnn'nnATC
Cuts at: 6 1560 3597 6
Size: 1554 2037 455
Fragments arranged by size:
2037 1554 455
BsaHI Gr'CG_yC
Cuts at: 1477 1725 2427 3923 1477
Size: 248 702 1496 1600
Fragments arranged by size:
1600 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1235 1756 2434 2708 2855 3266 1235
Size: 521 678 274 147 411 2015
Fragments arranged by size:
2015 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 257 287 257
Size: 30 4016
BseYI C'CCAG_C
Cuts at: 1471 2229 2806 3302 1471
Size: 758 577 496 2215
Fragments arranged by size:
2215 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1588 1634 2842 104
Size: 1484 46 1208 1308
Fragments arranged by size:
1484 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1060 1838 2028 2820 1060
Size: 778 190 792 2286
Fragments arranged by size:
2286 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1060 1671 1764 1838 2028 2090 2820 1060
Size: 611 93 74 190 62 730 2286
Fragments arranged by size:
2286 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3266 3266
Size: 4046
BspHI T'CATG_A
Cuts at: 3222 3222
Size: 4046
BspMI ACCTGCnnnn'nnnn_
Cuts at: 481 1096 1310 1612 1993 481
Size: 615 214 302 381 2534
Fragments arranged by size:
2534 615 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2338 2392 3876 4041 50
Size: 2288 54 1484 165 55
Fragments arranged by size:
2288 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 303 491 1958 303
Size: 188 1467 2391
Fragments arranged by size:
2391 1467 188
BsrFI r'CCGG_y
Cuts at: 2044 2225 2434 2044
Size: 181 209 3656
Fragments arranged by size:
3656 209 181
BssSI C'ACGA_G
Cuts at: 2317 2675 2317
Size: 358 3688
BstAPI GCAn_nnn'nTGC
Cuts at: 1423 1423
Size: 4046
BstBI TT'CG_AA
Cuts at: 2407 2407
Size: 4046
BstXI CCAn_nnnn'nTGG
Cuts at: 372 1002 372
Size: 630 3416
BstYI r'GATC_y
Cuts at: 1 892 1071 1538 1561 1896 2142 3154 3240
Size: 891 179 467 23 335 246 1012 86
Cuts at: 3240 3252 3598 1
Size: 12 346 449
Fragments arranged by size:
1012 891 467 449 346 335 246 179
86 23 12
BtgI C'CryG_G
Cuts at: 257 564 257
Size: 307 3739
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1996 2177 2340 1996
Size: 181 163 3702
Fragments arranged by size:
3702 181 163
BtsI GCAGTG_nn'
Cuts at: 1289 1289
Size: 4046
DraI TTT'AAA
Cuts at: 1045 1045
Size: 4046
DrdI GACnn_nn'nnGTC
Cuts at: 1752 2610 1752
Size: 858 3188
EaeI y'GGCC_r
Cuts at: 1004 1631 1805 2196 2223 3568 1004
Size: 627 174 391 27 1345 1482
Fragments arranged by size:
1482 1345 627 391 174 27
EagI C'GGCC_G
Cuts at: 1631 1631
Size: 4046
EarI CTCTTCn'nnn_
Cuts at: 305 752 869 2069 2279 305
Size: 447 117 1200 210 2072
Fragments arranged by size:
2072 1200 447 210 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2254 2564 2710 3856 2254
Size: 310 146 1146 2444
Fragments arranged by size:
2444 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1058 1058
Size: 4046
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 284 1871 2303 3050 3318 260
Size: 24 1587 432 747 268 988
Fragments arranged by size:
1587 988 747 432 268 24
EcoO109I rG'GnC_Cy
Cuts at: 334 361 1185 334
Size: 27 824 3195
Fragments arranged by size:
3195 824 27
EcoRI G'AATT_C
Cuts at: 1050 1050
Size: 4046
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 336 2065 336
Size: 1729 2317
FspI TGC'GCA
Cuts at: 1827 1827
Size: 4046
HaeII r_GCGC'y
Cuts at: 1480 1728 2750 3729 1480
Size: 248 1022 979 1797
Fragments arranged by size:
1797 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 257 289 745 777 951 983 1888 1918 1920
Size: 32 456 32 174 32 905 30 2
Cuts at: 1920 1950 257
Size: 30 2353
Fragments arranged by size:
2353 905 456 174 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 276 1085 276
Size: 809 3237
KasI G'GCGC_C
Cuts at: 1476 1724 1476
Size: 248 3798
KpnI G_GTAC'C
Cuts at: 1066 1066
Size: 4046
MluI A'CGCG_T
Cuts at: 3479 3479
Size: 4046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2717 2901 2717
Size: 184 3862
MscI TGG'CCA
Cuts at: 1006 1807 1006
Size: 801 3245
MslI CAynn'nnrTG
Cuts at: 127 1000 2035 2162 127
Size: 873 1035 127 2011
Fragments arranged by size:
2011 1035 873 127
MspA1I CmG'CkG
Cuts at: 249 481 566 1157 1471 1831 2844 3089 249
Size: 232 85 591 314 360 1013 245 1206
Fragments arranged by size:
1206 1013 591 360 314 245 232 85
NaeI GCC'GGC
Cuts at: 2227 2227
Size: 4046
NarI GG'CG_CC
Cuts at: 1477 1725 1477
Size: 248 3798
NcoI C'CATG_G
Cuts at: 257 257
Size: 4046
NdeI CA'TA_TG
Cuts at: 470 657 470
Size: 187 3859
NgoMIV G'CCGG_C
Cuts at: 2225 2225
Size: 4046
NheI G'CTAG_C
Cuts at: 798 798
Size: 4046
NspI r_CATG'y
Cuts at: 182 648 1099 1599 2506 182
Size: 466 451 500 907 1722
Fragments arranged by size:
1722 907 500 466 451
PciI A'CATG_T
Cuts at: 178 2502 178
Size: 2324 1722
PfoI T'CCnGG_A
Cuts at: 291 291
Size: 4046
PmeI GTTT'AAAC
Cuts at: 1045 1045
Size: 4046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3185 3217 3185
Size: 32 4014
PshAI GACnn'nnGTC
Cuts at: 210 210
Size: 4046
PsiI TTA'TAA
Cuts at: 532 532
Size: 4046
PstI C_TGCA'G
Cuts at: 212 1093 1778 212
Size: 881 685 2480
Fragments arranged by size:
2480 881 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4046
PvuII CAG'CTG
Cuts at: 249 481 1471 1831 249
Size: 232 990 360 2464
Fragments arranged by size:
2464 990 360 232
RsrII CG'GwC_CG
Cuts at: 2241 2241
Size: 4046
SacI G_AGCT'C
Cuts at: 1060 1060
Size: 4046
SacII CC_GC'GG
Cuts at: 567 567
Size: 4046
SalI G'TCGA_C
Cuts at: 1083 1083
Size: 4046
SapI GCTCTTCn'nnn_
Cuts at: 2069 2279 2069
Size: 210 3836
SbfI CC_TGCA'GG
Cuts at: 1093 1093
Size: 4046
ScaI AGT'ACT
Cuts at: 3563 3563
Size: 4046
SfcI C'TryA_G
Cuts at: 33 208 833 1089 1774 2767 2958 33
Size: 175 625 256 685 993 191 1121
Fragments arranged by size:
1121 993 685 625 256 191 175
SfoI GGC'GCC
Cuts at: 1478 1726 1478
Size: 248 3798
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
SmaI CCC'GGG
Cuts at: 1068 1068
Size: 4046
SmlI C'TyrA_G
Cuts at: 461 1201 2116 2608 2870 461
Size: 740 915 492 262 1637
Fragments arranged by size:
1637 915 740 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4046
SphI G_CATG'C
Cuts at: 1099 1599 1099
Size: 500 3546
StyI C'CwwG_G
Cuts at: 257 1180 1534 257
Size: 923 354 2769
Fragments arranged by size:
2769 923 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 571 1629 1762 2221 2430 571
Size: 1058 133 459 209 2187
Fragments arranged by size:
2187 1058 459 209 133
TatI w'GTAC_w
Cuts at: 465 3561 465
Size: 3096 950
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3563 3595 3563
Size: 32 4014
Tth111I GACn'n_nGTC
Cuts at: 1843 1843
Size: 4046
XbaI T'CTAG_A
Cuts at: 1077 1077
Size: 4046
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1014 1541 1014
Size: 527 3519
XmaI C'CCGG_G
Cuts at: 1066 1066
Size: 4046
XmnI GAAnn'nnTTC
Cuts at: 596 596
Size: 4046
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NspI PciI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
Bpu10I BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI