Restriction map for
pF1KB9273
(Circular) (Six-base) MAPSORT of: pF1KB9273.seq Check: 5621 from: 1 to: 4241
pF1KB9273 4241 bp
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AccI GT'mk_AC
Cuts at: 892 1279 892
Size: 387 3854
Acc65I G'GTAC_C
Cuts at: 1257 1257
Size: 4241
AclI AA'CG_TT
Cuts at: 4081 4081
Size: 4241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 793 2066 2498 3245 3513 730
Size: 63 1273 432 747 268 1458
Fragments arranged by size:
1458 1273 747 432 268 63
AflIII A'CryG_T
Cuts at: 971 1593 2697 3674 971
Size: 622 1104 977 1538
Fragments arranged by size:
1538 1104 977 622
AgeI A'CCGG_T
Cuts at: 2629 2629
Size: 4241
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1061 1143 1061
Size: 82 4159
AlwNI CAG_nnn'CTG
Cuts at: 816 1546 3113 816
Size: 730 1567 1944
Fragments arranged by size:
1944 1567 730
ApaLI G'TGCA_C
Cuts at: 1036 3011 1036
Size: 1975 2266
ApoI r'AATT_y
Cuts at: 424 789 1245 3594 3850 424
Size: 365 456 2349 256 815
Fragments arranged by size:
2349 815 456 365 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4241
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
AvaI C'yCGr_G
Cuts at: 1261 1261
Size: 4241
BamHI G'GATC_C
Cuts at: 1266 1266
Size: 4241
BanI G'GyrC_C
Cuts at: 1257 1671 1919 1954 1257
Size: 414 248 35 3544
Fragments arranged by size:
3544 414 248 35
BanII G_rGCy'C
Cuts at: 935 1255 2285 935
Size: 320 1030 2891
Fragments arranged by size:
2891 1030 320
BbeI G_GCGC'C
Cuts at: 1675 1923 1675
Size: 248 3993
BbsI GAAGACnn'nnnn_
Cuts at: 857 857
Size: 4241
BbvCI CC'TCA_GC
Cuts at: 178 1021 178
Size: 843 3398
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4207
BciVI GTATCCnnnnn_n'
Cuts at: 2139 2900 4241 2139
Size: 761 1341 2139
Fragments arranged by size:
2139 1341 761
BclI T'GATC_A
Cuts at: 1761 1761
Size: 4241
BglI GCCn_nnn'nGGC
Cuts at: 112 552 112
Size: 440 3801
BglII A'GATC_T
Cuts at: 293 1756 3793 293
Size: 1463 2037 741
Fragments arranged by size:
2037 1463 741
BlpI GC'TnA_GC
Cuts at: 1353 1353
Size: 4241
Bme1580I G_kGCm'C
Cuts at: 1040 1866 1959 3015 1040
Size: 826 93 1056 2266
Fragments arranged by size:
2266 1056 826 93
BmgBI CAC'GTC
Cuts at: 126 972 126
Size: 846 3395
BmrI ACTGGGnnnn_n'
Cuts at: 231 1453 1534 1869 4013 231
Size: 1222 81 335 2144 459
Fragments arranged by size:
2144 1222 459 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2608 2608
Size: 4241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 1187 583
Size: 604 3637
Bpu10I CC'TnA_GC
Cuts at: 178 1021 178
Size: 843 3398
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1417 2296 2788 3086 1417
Size: 879 492 298 2572
Fragments arranged by size:
2572 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3945 3945
Size: 4241
BsaAI yAC'GTr
Cuts at: 327 942 1594 327
Size: 615 652 2974
Fragments arranged by size:
2974 652 615
BsaBI GATnn'nnATC
Cuts at: 6 1755 3792 6
Size: 1749 2037 455
Fragments arranged by size:
2037 1749 455
BsaHI Gr'CG_yC
Cuts at: 1672 1920 2622 4118 1672
Size: 248 702 1496 1795
Fragments arranged by size:
1795 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1430 1951 2629 2903 3050 3461 1430
Size: 521 678 274 147 411 2210
Fragments arranged by size:
2210 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 586 678 1066 586
Size: 92 388 3761
Fragments arranged by size:
3761 388 92
BseYI C'CCAG_C
Cuts at: 999 1180 1223 1666 2424 3001 3497 999
Size: 181 43 443 758 577 496 1743
Fragments arranged by size:
1743 758 577 496 443 181 43
BsiEI CG_ry'CG
Cuts at: 104 1783 1829 3037 104
Size: 1679 46 1208 1308
Fragments arranged by size:
1679 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1040 1255 2033 2223 3015 1040
Size: 215 778 190 792 2266
Fragments arranged by size:
2266 792 778 215 190
Bsp1286I G_dGCh'C
Cuts at: 935 1040 1255 1866 1959 2033 2223 2285 3015
Size: 105 215 611 93 74 190 62 730
Cuts at: 3015 935
Size: 2161
Fragments arranged by size:
2161 730 611 215 190 105 93 74
62
BspEI T'CCGG_A
Cuts at: 3461 3461
Size: 4241
BspHI T'CATG_A
Cuts at: 3417 3417
Size: 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1025 1291 1505 1807 2188 1025
Size: 266 214 302 381 3078
Fragments arranged by size:
3078 381 302 266 214
BsrBI CCG'CTC
Cuts at: 50 2533 2587 4071 4236 50
Size: 2483 54 1484 165 55
Fragments arranged by size:
2483 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 473 2153 473
Size: 1680 2561
BsrFI r'CCGG_y
Cuts at: 2239 2420 2629 2239
Size: 181 209 3851
Fragments arranged by size:
3851 209 181
BsrGI T'GTAC_A
Cuts at: 1007 1007
Size: 4241
BssSI C'ACGA_G
Cuts at: 2512 2870 2512
Size: 358 3883
BstAPI GCAn_nnn'nTGC
Cuts at: 117 1618 117
Size: 1501 2740
BstBI TT'CG_AA
Cuts at: 2602 2602
Size: 4241
BstEII G'GTnAC_C
Cuts at: 190 190
Size: 4241
BstYI r'GATC_y
Cuts at: 1 293 1170 1266 1733 1756 2091 2337 3349
Size: 292 877 96 467 23 335 246 1012
Cuts at: 3349 3435 3447 3793 1
Size: 86 12 346 449
Fragments arranged by size:
1012 877 467 449 346 335 292 246
96 86 23 12
BstZ17I GTA'TAC
Cuts at: 893 893
Size: 4241
Bsu36I CC'TnA_GG
Cuts at: 661 661
Size: 4241
BtgI C'CryG_G
Cuts at: 161 161
Size: 4241
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2191 2372 2535 2191
Size: 181 163 3897
Fragments arranged by size:
3897 181 163
BtsI GCAGTG_nn'
Cuts at: 1484 1484
Size: 4241
DraI TTT'AAA
Cuts at: 618 1240 618
Size: 622 3619
DraIII CAC_nnn'GTG
Cuts at: 129 129
Size: 4241
DrdI GACnn_nn'nnGTC
Cuts at: 1947 2805 1947
Size: 858 3383
EaeI y'GGCC_r
Cuts at: 164 1230 1826 2000 2391 2418 3763 164
Size: 1066 596 174 391 27 1345 642
Fragments arranged by size:
1345 1066 642 596 391 174 27
EagI C'GGCC_G
Cuts at: 1826 1826
Size: 4241
EarI CTCTTCn'nnn_
Cuts at: 778 2264 2474 778
Size: 1486 210 2545
Fragments arranged by size:
2545 1486 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 198 1096 2449 2759 2905 4051 198
Size: 898 1353 310 146 1146 388
Fragments arranged by size:
1353 1146 898 388 310 146
EcoICRI GAG'CTC
Cuts at: 1253 1253
Size: 4241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 730 793 1187 2066 2498 3245 3513 583
Size: 147 63 394 879 432 747 268 1311
Fragments arranged by size:
1311 879 747 432 394 268 147 63
EcoO109I rG'GnC_Cy
Cuts at: 1014 1380 1014
Size: 366 3875
EcoRI G'AATT_C
Cuts at: 1245 1245
Size: 4241
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 4241
FspI TGC'GCA
Cuts at: 2022 2022
Size: 4241
HaeII r_GCGC'y
Cuts at: 161 1675 1923 2945 3924 161
Size: 1514 248 1022 979 478
Fragments arranged by size:
1514 1022 979 478 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 378 410 2083 2113 2115 2145 378
Size: 32 1673 30 2 30 2474
Fragments arranged by size:
2474 1673 32 30 30 2
HincII GTy'rAC
Cuts at: 1280 1280
Size: 4241
KasI G'GCGC_C
Cuts at: 1671 1919 1671
Size: 248 3993
KpnI G_GTAC'C
Cuts at: 1261 1261
Size: 4241
MluI A'CGCG_T
Cuts at: 3674 3674
Size: 4241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2912 3096 2912
Size: 184 4057
MscI TGG'CCA
Cuts at: 1232 2002 1232
Size: 770 3471
MslI CAynn'nnrTG
Cuts at: 766 2230 2357 766
Size: 1464 127 2650
Fragments arranged by size:
2650 1464 127
MspA1I CmG'CkG
Cuts at: 607 1164 1352 1666 2026 3039 3284 607
Size: 557 188 314 360 1013 245 1564
Fragments arranged by size:
1564 1013 557 360 314 245 188
NaeI GCC'GGC
Cuts at: 2422 2422
Size: 4241
NarI GG'CG_CC
Cuts at: 1672 1920 1672
Size: 248 3993
NgoMIV G'CCGG_C
Cuts at: 2420 2420
Size: 4241
NspI r_CATG'y
Cuts at: 771 1294 1794 2701 771
Size: 523 500 907 2311
Fragments arranged by size:
2311 907 523 500
PciI A'CATG_T
Cuts at: 2697 2697
Size: 4241
PflMI CCAn_nnn'nTGG
Cuts at: 1116 1116
Size: 4241
PfoI T'CCnGG_A
Cuts at: 1073 1073
Size: 4241
PmeI GTTT'AAAC
Cuts at: 1240 1240
Size: 4241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 211 243 3380 3412 211
Size: 32 3137 32 1040
Fragments arranged by size:
3137 1040 32 32
PpuMI rG'GwC_Cy
Cuts at: 1014 1014
Size: 4241
PstI C_TGCA'G
Cuts at: 821 1288 1973 821
Size: 467 685 3089
Fragments arranged by size:
3089 685 467
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4241
PvuII CAG'CTG
Cuts at: 607 1666 2026 607
Size: 1059 360 2822
Fragments arranged by size:
2822 1059 360
RsrII CG'GwC_CG
Cuts at: 2436 2436
Size: 4241
SacI G_AGCT'C
Cuts at: 1255 1255
Size: 4241
SalI G'TCGA_C
Cuts at: 1278 1278
Size: 4241
SapI GCTCTTCn'nnn_
Cuts at: 2264 2474 2264
Size: 210 4031
SbfI CC_TGCA'GG
Cuts at: 1288 1288
Size: 4241
ScaI AGT'ACT
Cuts at: 3758 3758
Size: 4241
SexAI A'CCwGG_T
Cuts at: 194 828 194
Size: 634 3607
SfcI C'TryA_G
Cuts at: 33 817 1284 1969 2962 3153 33
Size: 784 467 685 993 191 1121
Fragments arranged by size:
1121 993 784 685 467 191
SfoI GGC'GCC
Cuts at: 1673 1921 1673
Size: 248 3993
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
SmaI CCC'GGG
Cuts at: 1263 1263
Size: 4241
SmlI C'TyrA_G
Cuts at: 1396 2311 2803 3065 1396
Size: 915 492 262 2572
Fragments arranged by size:
2572 915 492 262
SnaBI TAC'GTA
Cuts at: 327 327
Size: 4241
SpeI A'CTAG_T
Cuts at: 67 1122 67
Size: 1055 3186
SphI G_CATG'C
Cuts at: 1294 1794 1294
Size: 500 3741
StuI AGG'CCT
Cuts at: 115 115
Size: 4241
StyI C'CwwG_G
Cuts at: 896 1375 1729 896
Size: 479 354 3408
Fragments arranged by size:
3408 479 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1013 1824 1957 2416 2625 1013
Size: 811 133 459 209 2629
Fragments arranged by size:
2629 811 459 209 133
TatI w'GTAC_w
Cuts at: 250 1007 3756 250
Size: 757 2749 735
Fragments arranged by size:
2749 757 735
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 826 858 3758 3790 826
Size: 32 2900 32 1277
Fragments arranged by size:
2900 1277 32 32
Tth111I GACn'n_nGTC
Cuts at: 830 958 2038 830
Size: 128 1080 3033
Fragments arranged by size:
3033 1080 128
XbaI T'CTAG_A
Cuts at: 1272 1272
Size: 4241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 678 1220 1736 678
Size: 542 516 3183
Fragments arranged by size:
3183 542 516
XmaI C'CCGG_G
Cuts at: 1261 1261
Size: 4241
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BfrBI BmtI BsaXI BsgI BsiWI
BsmI BsmBI BssHII BstXI ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SrfI
SspI SwaI XhoI XmnI ZraI