Restriction map for
pF1KB9268
(Circular) (Six-base) MAPSORT of: pF1KB9268.seq Check: 9663 from: 1 to: 4841
pF1KB9268 4841 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AccI GT'mk_AC
Cuts at: 1879 1879
Size: 4841
Acc65I G'GTAC_C
Cuts at: 1857 1857
Size: 4841
AclI AA'CG_TT
Cuts at: 4681 4681
Size: 4841
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 509 877 2666 3098 3845 4113 340
Size: 169 368 1789 432 747 268 1068
Fragments arranged by size:
1789 1068 747 432 368 268 169
AflIII A'CryG_T
Cuts at: 189 2193 3297 4274 189
Size: 2004 1104 977 756
Fragments arranged by size:
2004 1104 977 756
AgeI A'CCGG_T
Cuts at: 3229 3229
Size: 4841
AhdI GACnn_n'nnGTC
Cuts at: 1219 1219
Size: 4841
AlwNI CAG_nnn'CTG
Cuts at: 1035 2146 3713 1035
Size: 1111 1567 2163
Fragments arranged by size:
2163 1567 1111
ApaLI G'TGCA_C
Cuts at: 3611 3611
Size: 4841
ApoI r'AATT_y
Cuts at: 1540 1845 4194 4450 1540
Size: 305 2349 256 1931
Fragments arranged by size:
2349 1931 305 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4841
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4841
AvaI C'yCGr_G
Cuts at: 1861 1861
Size: 4841
BamHI G'GATC_C
Cuts at: 1866 1866
Size: 4841
BanI G'GyrC_C
Cuts at: 303 1812 1857 2271 2519 2554 303
Size: 1509 45 414 248 35 2590
Fragments arranged by size:
2590 1509 414 248 45 35
BanII G_rGCy'C
Cuts at: 1772 1855 2885 1772
Size: 83 1030 3728
Fragments arranged by size:
3728 1030 83
BbeI G_GCGC'C
Cuts at: 2275 2523 2275
Size: 248 4593
BbsI GAAGACnn'nnnn_
Cuts at: 212 515 212
Size: 303 4538
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 928 962 90
Size: 34 804 34 3969
Fragments arranged by size:
3969 804 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 671 2739 3500 4841 671
Size: 2068 761 1341 671
Fragments arranged by size:
2068 1341 761 671
BclI T'GATC_A
Cuts at: 1137 1274 2361 1137
Size: 137 1087 3617
Fragments arranged by size:
3617 1087 137
BglII A'GATC_T
Cuts at: 2356 4393 2356
Size: 2037 2804
BlpI GC'TnA_GC
Cuts at: 1953 1953
Size: 4841
Bme1580I G_kGCm'C
Cuts at: 308 1310 2466 2559 3615 308
Size: 1002 1156 93 1056 1534
Fragments arranged by size:
1534 1156 1056 1002 93
BmrI ACTGGGnnnn_n'
Cuts at: 463 562 2053 2134 2469 4613 463
Size: 99 1491 81 335 2144 691
Fragments arranged by size:
2144 1491 691 335 99 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 528 3208 528
Size: 2680 2161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2017 2896 3388 3686 2017
Size: 879 492 298 3172
Fragments arranged by size:
3172 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1795 4545 1795
Size: 2750 2091
BsaAI yAC'GTr
Cuts at: 2194 2194
Size: 4841
BsaBI GATnn'nnATC
Cuts at: 6 802 2355 4392 6
Size: 796 1553 2037 455
Fragments arranged by size:
2037 1553 796 455
BsaHI Gr'CG_yC
Cuts at: 2272 2520 3222 4718 2272
Size: 248 702 1496 2395
Fragments arranged by size:
2395 1496 702 248
BsaWI w'CCGG_w
Cuts at: 300 2030 2551 3229 3503 3650 4061 300
Size: 1730 521 678 274 147 411 1080
Fragments arranged by size:
1730 1080 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 629 632 787 629
Size: 3 155 4683
Fragments arranged by size:
4683 155 3
BseYI C'CCAG_C
Cuts at: 176 862 1286 2266 3024 3601 4097 176
Size: 686 424 980 758 577 496 920
Fragments arranged by size:
980 920 758 686 577 496 424
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1673 1673
Size: 4841
BsiEI CG_ry'CG
Cuts at: 104 2383 2429 3637 104
Size: 2279 46 1208 1308
Fragments arranged by size:
2279 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1772 1855 2633 2823 3615 1772
Size: 83 778 190 792 2998
Fragments arranged by size:
2998 792 778 190 83
BsmI GAATG_Cn'
Cuts at: 249 1129 1759 249
Size: 880 630 3331
Fragments arranged by size:
3331 880 630
BsmBI CGTCTCn'nnnn_
Cuts at: 1002 1002
Size: 4841
Bsp1286I G_dGCh'C
Cuts at: 308 1310 1772 1855 2466 2559 2633 2823 2885
Size: 1002 462 83 611 93 74 190 62
Cuts at: 2885 3615 308
Size: 730 1534
Fragments arranged by size:
1534 1002 730 611 462 190 93 83
74 62
BspEI T'CCGG_A
Cuts at: 4061 4061
Size: 4841
BspHI T'CATG_A
Cuts at: 267 608 4017 267
Size: 341 3409 1091
Fragments arranged by size:
3409 1091 341
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1891 2105 2407 2788 1891
Size: 214 302 381 3944
Fragments arranged by size:
3944 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3133 3187 4671 4836 50
Size: 3083 54 1484 165 55
Fragments arranged by size:
3083 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2753 2753
Size: 4841
BsrFI r'CCGG_y
Cuts at: 2839 3020 3229 2839
Size: 181 209 4451
Fragments arranged by size:
4451 209 181
BsrGI T'GTAC_A
Cuts at: 435 1392 435
Size: 957 3884
BssSI C'ACGA_G
Cuts at: 3112 3470 3112
Size: 358 4483
BstAPI GCAn_nnn'nTGC
Cuts at: 2218 2218
Size: 4841
BstBI TT'CG_AA
Cuts at: 3202 3202
Size: 4841
BstXI CCAn_nnnn'nTGG
Cuts at: 1294 1823 1294
Size: 529 4312
BstYI r'GATC_y
Cuts at: 1 127 1866 2333 2356 2691 2937 3949 4035
Size: 126 1739 467 23 335 246 1012 86
Cuts at: 4035 4047 4393 1
Size: 12 346 449
Fragments arranged by size:
1739 1012 467 449 346 335 246 126
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 701 701
Size: 4841
BtgI C'CryG_G
Cuts at: 315 1235 315
Size: 920 3921
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2791 2972 3135 2791
Size: 181 163 4497
Fragments arranged by size:
4497 181 163
BtsI GCAGTG_nn'
Cuts at: 2084 2084
Size: 4841
DraI TTT'AAA
Cuts at: 1840 1840
Size: 4841
DrdI GACnn_nn'nnGTC
Cuts at: 2547 3405 2547
Size: 858 3983
EaeI y'GGCC_r
Cuts at: 332 344 599 2426 2600 2991 3018 4363 332
Size: 12 255 1827 174 391 27 1345 810
Fragments arranged by size:
1827 1345 810 391 255 174 27 12
EagI C'GGCC_G
Cuts at: 2426 2426
Size: 4841
EarI CTCTTCn'nnn_
Cuts at: 344 1126 2864 3074 344
Size: 782 1738 210 2111
Fragments arranged by size:
2111 1738 782 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3049 3359 3505 4651 3049
Size: 310 146 1146 3239
Fragments arranged by size:
3239 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1770 1853 1770
Size: 83 4758
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 509 877 2666 3098 3845 4113 340
Size: 169 368 1789 432 747 268 1068
Fragments arranged by size:
1789 1068 747 432 368 268 169
EcoO109I rG'GnC_Cy
Cuts at: 1980 1980
Size: 4841
EcoRI G'AATT_C
Cuts at: 1845 1845
Size: 4841
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 883 2860 883
Size: 1977 2864
FspI TGC'GCA
Cuts at: 2622 2622
Size: 4841
HaeII r_GCGC'y
Cuts at: 2275 2523 3545 4524 2275
Size: 248 1022 979 2592
Fragments arranged by size:
2592 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 708 740 766 798 1170 1202 1205 1237 2683
Size: 32 26 32 372 32 3 32 1446
Cuts at: 2683 2713 2715 2745 708
Size: 30 2 30 2804
Fragments arranged by size:
2804 1446 372 32 32 32 32 30
30 26 3 2
HincII GTy'rAC
Cuts at: 122 672 1880 122
Size: 550 1208 3083
Fragments arranged by size:
3083 1208 550
KasI G'GCGC_C
Cuts at: 2271 2519 2271
Size: 248 4593
KpnI G_GTAC'C
Cuts at: 1861 1861
Size: 4841
MluI A'CGCG_T
Cuts at: 4274 4274
Size: 4841
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1070 1478 3512 3696 1070
Size: 408 2034 184 2215
Fragments arranged by size:
2215 2034 408 184
MscI TGG'CCA
Cuts at: 601 2602 601
Size: 2001 2840
MslI CAynn'nnrTG
Cuts at: 284 650 2830 2957 284
Size: 366 2180 127 2168
Fragments arranged by size:
2180 2168 366 127
MspA1I CmG'CkG
Cuts at: 180 1153 1952 2266 2626 3639 3884 180
Size: 973 799 314 360 1013 245 1137
Fragments arranged by size:
1137 1013 973 799 360 314 245
NaeI GCC'GGC
Cuts at: 3022 3022
Size: 4841
NarI GG'CG_CC
Cuts at: 2272 2520 2272
Size: 248 4593
NcoI C'CATG_G
Cuts at: 315 315
Size: 4841
NgoMIV G'CCGG_C
Cuts at: 3020 3020
Size: 4841
NspI r_CATG'y
Cuts at: 193 1894 2394 3301 193
Size: 1701 500 907 1733
Fragments arranged by size:
1733 1701 907 500
PasI CC'CwG_GG
Cuts at: 956 956
Size: 4841
PciI A'CATG_T
Cuts at: 189 3297 189
Size: 3108 1733
PfoI T'CCnGG_A
Cuts at: 986 986
Size: 4841
PmeI GTTT'AAAC
Cuts at: 1840 1840
Size: 4841
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3980 4012 3980
Size: 32 4809
PshAI GACnn'nnGTC
Cuts at: 143 143
Size: 4841
PsiI TTA'TAA
Cuts at: 1564 1735 1564
Size: 171 4670
PstI C_TGCA'G
Cuts at: 185 627 1888 2573 185
Size: 442 1261 685 2453
Fragments arranged by size:
2453 1261 685 442
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4841
PvuII CAG'CTG
Cuts at: 180 1153 2266 2626 180
Size: 973 1113 360 2395
Fragments arranged by size:
2395 1113 973 360
RsrII CG'GwC_CG
Cuts at: 3036 3036
Size: 4841
SacI G_AGCT'C
Cuts at: 1772 1855 1772
Size: 83 4758
SalI G'TCGA_C
Cuts at: 1878 1878
Size: 4841
SapI GCTCTTCn'nnn_
Cuts at: 344 2864 3074 344
Size: 2520 210 2111
Fragments arranged by size:
2520 2111 210
SbfI CC_TGCA'GG
Cuts at: 1888 1888
Size: 4841
ScaI AGT'ACT
Cuts at: 4358 4358
Size: 4841
SfcI C'TryA_G
Cuts at: 33 181 623 1884 2569 3562 3753 33
Size: 148 442 1261 685 993 191 1121
Fragments arranged by size:
1261 1121 993 685 442 191 148
SfoI GGC'GCC
Cuts at: 2273 2521 2273
Size: 248 4593
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4841
SmaI CCC'GGG
Cuts at: 1863 1863
Size: 4841
SmlI C'TyrA_G
Cuts at: 1996 2911 3403 3665 1996
Size: 915 492 262 3172
Fragments arranged by size:
3172 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4841
SphI G_CATG'C
Cuts at: 1894 2394 1894
Size: 500 4341
SspI AAT'ATT
Cuts at: 1022 1022
Size: 4841
StuI AGG'CCT
Cuts at: 699 699
Size: 4841
StyI C'CwwG_G
Cuts at: 315 329 1635 1975 2329 315
Size: 14 1306 340 354 2827
Fragments arranged by size:
2827 1306 354 340 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1300 1454 1800 2424 2557 3016 3225 1300
Size: 154 346 624 133 459 209 2916
Fragments arranged by size:
2916 624 459 346 209 154 133
TatI w'GTAC_w
Cuts at: 435 1392 4356 435
Size: 957 2964 920
Fragments arranged by size:
2964 957 920
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4358 4390 4358
Size: 32 4809
Tth111I GACn'n_nGTC
Cuts at: 2638 2638
Size: 4841
XbaI T'CTAG_A
Cuts at: 1872 1872
Size: 4841
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1342 2336 1342
Size: 994 3847
XmaI C'CCGG_G
Cuts at: 1861 1861
Size: 4841
XmnI GAAnn'nnTTC
Cuts at: 353 1667 353
Size: 1314 3527
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BsiWI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PpuMI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI