Restriction map for
pF1KB9171
(Circular) (Six-base) MAPSORT of: pF1KB9171.seq Check: 3815 from: 1 to: 5120
pF1KB9171 5120 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 848 966 1518 848
Size: 118 552 4450
Fragments arranged by size:
4450 552 118
AatII G_ACGT'C
Cuts at: 1394 2039 1394
Size: 645 4475
AccI GT'mk_AC
Cuts at: 2158 2158
Size: 5120
Acc65I G'GTAC_C
Cuts at: 2136 2136
Size: 5120
AclI AA'CG_TT
Cuts at: 4960 4960
Size: 5120
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2945 3377 4124 4392 2945
Size: 432 747 268 3673
Fragments arranged by size:
3673 747 432 268
AflIII A'CryG_T
Cuts at: 2472 3576 4553 2472
Size: 1104 977 3039
Fragments arranged by size:
3039 1104 977
AgeI A'CCGG_T
Cuts at: 1762 3508 1762
Size: 1746 3374
AlwNI CAG_nnn'CTG
Cuts at: 363 433 972 981 1068 1561 1623 2046 2425
Size: 70 539 9 87 493 62 423 379
Cuts at: 2425 3992 363
Size: 1567 1491
Fragments arranged by size:
1567 1491 539 493 423 379 87 70
62 9
ApaI G_GGCC'C
Cuts at: 1870 1870
Size: 5120
ApaLI G'TGCA_C
Cuts at: 295 625 3890 295
Size: 330 3265 1525
Fragments arranged by size:
3265 1525 330
ApoI r'AATT_y
Cuts at: 2124 4473 4729 2124
Size: 2349 256 2515
Fragments arranged by size:
2515 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5120
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5120
AvaI C'yCGr_G
Cuts at: 169 396 502 1463 1973 2140 169
Size: 227 106 961 510 167 3149
Fragments arranged by size:
3149 961 510 227 167 106
AvrII C'CTAG_G
Cuts at: 339 2004 339
Size: 1665 3455
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 384 417 384
Size: 33 5087
BamHI G'GATC_C
Cuts at: 602 641 1964 2145 602
Size: 39 1323 181 3577
Fragments arranged by size:
3577 1323 181 39
BanI G'GyrC_C
Cuts at: 506 689 1107 1992 2136 2550 2798 2833 506
Size: 183 418 885 144 414 248 35 2793
Fragments arranged by size:
2793 885 418 414 248 183 144 35
BanII G_rGCy'C
Cuts at: 1213 1297 1617 1870 2134 3164 1213
Size: 84 320 253 264 1030 3169
Fragments arranged by size:
3169 1030 320 264 253 84
BbeI G_GCGC'C
Cuts at: 1111 2554 2802 1111
Size: 1443 248 3429
Fragments arranged by size:
3429 1443 248
BciVI GTATCCnnnnn_n'
Cuts at: 324 3018 3779 5120 324
Size: 2694 761 1341 324
Fragments arranged by size:
2694 1341 761 324
BclI T'GATC_A
Cuts at: 2640 2640
Size: 5120
BglI GCCn_nnn'nGGC
Cuts at: 268 430 504 1865 268
Size: 162 74 1361 3523
Fragments arranged by size:
3523 1361 162 74
BglII A'GATC_T
Cuts at: 2635 4672 2635
Size: 2037 3083
BlpI GC'TnA_GC
Cuts at: 2232 2232
Size: 5120
Bme1580I G_kGCm'C
Cuts at: 299 509 629 1125 1146 1870 2745 2838 3894
Size: 210 120 496 21 724 875 93 1056
Cuts at: 3894 299
Size: 1525
Fragments arranged by size:
1525 1056 875 724 496 210 120 93
21
BmrI ACTGGGnnnn_n'
Cuts at: 627 2332 2413 2748 4892 627
Size: 1705 81 335 2144 855
Fragments arranged by size:
2144 1705 855 335 81
BmtI G_CTAG'C
Cuts at: 1960 1960
Size: 5120
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1959 3487 1959
Size: 1528 3592
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 485 1030 1048 1162 1538 1643 1954 2089 485
Size: 545 18 114 376 105 311 135 3516
Fragments arranged by size:
3516 545 376 311 135 114 105 18
Bpu10I CC'TnA_GC
Cuts at: 191 872 1774 191
Size: 681 902 3537
Fragments arranged by size:
3537 902 681
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2296 3175 3667 3965 2296
Size: 879 492 298 3451
Fragments arranged by size:
3451 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 322 891 4824 322
Size: 569 3933 618
Fragments arranged by size:
3933 618 569
BsaAI yAC'GTr
Cuts at: 822 2473 822
Size: 1651 3469
BsaBI GATnn'nnATC
Cuts at: 6 2634 4671 6
Size: 2628 2037 455
Fragments arranged by size:
2628 2037 455
BsaHI Gr'CG_yC
Cuts at: 380 1108 1391 2036 2551 2799 3501 4997 380
Size: 728 283 645 515 248 702 1496 503
Fragments arranged by size:
1496 728 702 645 515 503 283 248
BsaWI w'CCGG_w
Cuts at: 367 1762 2309 2830 3508 3782 3929 4340 367
Size: 1395 547 521 678 274 147 411 1147
Fragments arranged by size:
1395 1147 678 547 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1033 1063 2062 2092 1033
Size: 30 999 30 4061
Fragments arranged by size:
4061 999 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 295 364 1438 1483 1695 1951 295
Size: 69 1074 45 212 256 3464
Fragments arranged by size:
3464 1074 256 212 69 45
BseYI C'CCAG_C
Cuts at: 285 667 1071 1213 1842 1952 2545 3303 3880
Size: 382 404 142 629 110 593 758 577
Cuts at: 3880 4376 285
Size: 496 1029
Fragments arranged by size:
1029 758 629 593 577 496 404 382
142 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 554 820 1271 1466 1862 554
Size: 266 451 195 396 3812
Fragments arranged by size:
3812 451 396 266 195
BsiEI CG_ry'CG
Cuts at: 104 993 2662 2708 3916 104
Size: 889 1669 46 1208 1308
Fragments arranged by size:
1669 1308 1208 889 46
BsiHKAI G_wGCw'C
Cuts at: 299 461 629 1297 2134 2912 3102 3894 299
Size: 162 168 668 837 778 190 792 1525
Fragments arranged by size:
1525 837 792 778 668 190 168 162
Bsp1286I G_dGCh'C
Cuts at: 299 461 509 629 1125 1146 1213 1297 1617
Size: 162 48 120 496 21 67 84 320
Cuts at: 1617 1870 2134 2745 2838 2912 3102 3164 3894
Size: 253 264 611 93 74 190 62 730
Cuts at: 3894 299
Size: 1525
Fragments arranged by size:
1525 730 611 496 320 264 253 190
162 120 93 84 74 67 62 48
21
BspEI T'CCGG_A
Cuts at: 367 4340 367
Size: 3973 1147
BspHI T'CATG_A
Cuts at: 4296 4296
Size: 5120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 791 848 966 1518 2170 2384 2686 3067 791
Size: 57 118 552 652 214 302 381 2844
Fragments arranged by size:
2844 652 552 381 302 214 118 57
BsrBI CCG'CTC
Cuts at: 50 3412 3466 4950 5115 50
Size: 3362 54 1484 165 55
Fragments arranged by size:
3362 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1898 3032 1898
Size: 1134 3986
BsrFI r'CCGG_y
Cuts at: 985 1652 1762 3118 3299 3508 985
Size: 667 110 1356 181 209 2597
Fragments arranged by size:
2597 1356 667 209 181 110
BssHII G'CGCG_C
Cuts at: 413 413
Size: 5120
BssSI C'ACGA_G
Cuts at: 1450 3391 3749 1450
Size: 1941 358 2821
Fragments arranged by size:
2821 1941 358
BstAPI GCAn_nnn'nTGC
Cuts at: 843 2497 843
Size: 1654 3466
BstBI TT'CG_AA
Cuts at: 3481 3481
Size: 5120
BstEII G'GTnAC_C
Cuts at: 1075 1075
Size: 5120
BstYI r'GATC_y
Cuts at: 1 602 641 1964 2145 2612 2635 2970 3216
Size: 601 39 1323 181 467 23 335 246
Cuts at: 3216 4228 4314 4326 4672 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1323 1012 601 467 449 346 335 246
181 86 39 23 12
Bsu36I CC'TnA_GG
Cuts at: 920 1417 920
Size: 497 4623
BtgI C'CryG_G
Cuts at: 107 510 630 1608 1854 107
Size: 403 120 978 246 3373
Fragments arranged by size:
3373 978 403 246 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3070 3251 3414 3070
Size: 181 163 4776
Fragments arranged by size:
4776 181 163
BtsI GCAGTG_nn'
Cuts at: 408 752 1478 1747 2098 2363 408
Size: 344 726 269 351 265 3165
Fragments arranged by size:
3165 726 351 344 269 265
DraI TTT'AAA
Cuts at: 2119 2119
Size: 5120
DraIII CAC_nnn'GTG
Cuts at: 2109 2109
Size: 5120
DrdI GACnn_nn'nnGTC
Cuts at: 670 2826 3684 670
Size: 2156 858 2106
Fragments arranged by size:
2156 2106 858
EaeI y'GGCC_r
Cuts at: 143 422 513 557 633 983 990 1851 2705
Size: 279 91 44 76 350 7 861 854
Cuts at: 2705 2879 3270 3297 4642 143
Size: 174 391 27 1345 621
Fragments arranged by size:
1345 861 854 621 391 350 279 174
91 76 44 27 7
EagI C'GGCC_G
Cuts at: 990 2705 990
Size: 1715 3405
EarI CTCTTCn'nnn_
Cuts at: 272 1165 3143 3353 272
Size: 893 1978 210 2039
Fragments arranged by size:
2039 1978 893 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1054 3328 3638 3784 4930 1054
Size: 2274 310 146 1146 1244
Fragments arranged by size:
2274 1244 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1295 2132 1295
Size: 837 4283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 485 1030 1048 1162 1538 1643 1954 2089 2945
Size: 545 18 114 376 105 311 135 856
Cuts at: 2945 3377 4124 4392 485
Size: 432 747 268 1213
Fragments arranged by size:
1213 856 747 545 432 376 311 268
135 114 105 18
EcoNI CCTnn'n_nnAGG
Cuts at: 343 1420 1440 1933 2003 343
Size: 1077 20 493 70 3460
Fragments arranged by size:
3460 1077 493 70 20
EcoO109I rG'GnC_Cy
Cuts at: 336 762 915 1158 1473 1846 1866 1867 2008
Size: 426 153 243 315 373 20 1 141
Cuts at: 2008 2043 2259 336
Size: 35 216 3197
Fragments arranged by size:
3197 426 373 315 243 216 153 141
35 20 1
EcoRI G'AATT_C
Cuts at: 2124 2124
Size: 5120
EcoRV GAT'ATC
Cuts at: 1368 1368
Size: 5120
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3139 3139
Size: 5120
FspI TGC'GCA
Cuts at: 2901 2901
Size: 5120
HaeII r_GCGC'y
Cuts at: 198 364 711 1111 1843 2554 2802 3824 4803
Size: 166 347 400 732 711 248 1022 979
Cuts at: 4803 198
Size: 515
Fragments arranged by size:
1022 979 732 711 515 400 347 248
166
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 171 203 298 330 633 665 749 781 2962
Size: 32 95 32 303 32 84 32 2181
Cuts at: 2962 2992 2994 3024 171
Size: 30 2 30 2267
Fragments arranged by size:
2267 2181 303 95 84 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 780 2159 780
Size: 1379 3741
KasI G'GCGC_C
Cuts at: 1107 2550 2798 1107
Size: 1443 248 3429
Fragments arranged by size:
3429 1443 248
KpnI G_GTAC'C
Cuts at: 2140 2140
Size: 5120
MluI A'CGCG_T
Cuts at: 4553 4553
Size: 5120
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1422 3791 3975 1422
Size: 2369 184 2567
Fragments arranged by size:
2567 2369 184
MscI TGG'CCA
Cuts at: 515 559 635 1853 2881 515
Size: 44 76 1218 1028 2754
Fragments arranged by size:
2754 1218 1028 76 44
MslI CAynn'nnrTG
Cuts at: 695 3109 3236 695
Size: 2414 127 2579
Fragments arranged by size:
2579 2414 127
MspA1I CmG'CkG
Cuts at: 444 568 969 1198 1279 1345 1647 2067 2231
Size: 124 401 229 81 66 302 420 164
Cuts at: 2231 2545 2905 3918 4163 444
Size: 314 360 1013 245 1401
Fragments arranged by size:
1401 1013 420 401 360 314 302 245
229 164 124 81 66
NaeI GCC'GGC
Cuts at: 987 3301 987
Size: 2314 2806
NarI GG'CG_CC
Cuts at: 1108 2551 2799 1108
Size: 1443 248 3429
Fragments arranged by size:
3429 1443 248
NcoI C'CATG_G
Cuts at: 107 510 630 1854 107
Size: 403 120 1224 3373
Fragments arranged by size:
3373 1224 403 120
NgoMIV G'CCGG_C
Cuts at: 985 3299 985
Size: 2314 2806
NheI G'CTAG_C
Cuts at: 1956 1956
Size: 5120
NspI r_CATG'y
Cuts at: 577 2173 2673 3580 577
Size: 1596 500 907 2117
Fragments arranged by size:
2117 1596 907 500
PasI CC'CwG_GG
Cuts at: 1638 1638
Size: 5120
PciI A'CATG_T
Cuts at: 3576 3576
Size: 5120
PfoI T'CCnGG_A
Cuts at: 1301 1931 1301
Size: 630 4490
PmeI GTTT'AAAC
Cuts at: 2119 2119
Size: 5120
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4259 4291 4259
Size: 32 5088
PpuMI rG'GwC_Cy
Cuts at: 336 915 336
Size: 579 4541
PshAI GACnn'nnGTC
Cuts at: 334 334
Size: 5120
PspOMI G'GGCC_C
Cuts at: 1866 1866
Size: 5120
PstI C_TGCA'G
Cuts at: 359 443 977 1569 1865 2167 2852 359
Size: 84 534 592 296 302 685 2627
Fragments arranged by size:
2627 685 592 534 302 296 84
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5120
PvuII CAG'CTG
Cuts at: 444 568 969 1279 1647 2067 2545 2905 444
Size: 124 401 310 368 420 478 360 2659
Fragments arranged by size:
2659 478 420 401 368 360 310 124
RsrII CG'GwC_CG
Cuts at: 3315 3315
Size: 5120
SacI G_AGCT'C
Cuts at: 1297 2134 1297
Size: 837 4283
SalI G'TCGA_C
Cuts at: 2157 2157
Size: 5120
SanDI GG'GwC_CC
Cuts at: 915 915
Size: 5120
SapI GCTCTTCn'nnn_
Cuts at: 1165 3143 3353 1165
Size: 1978 210 2932
Fragments arranged by size:
2932 1978 210
SbfI CC_TGCA'GG
Cuts at: 1865 2167 1865
Size: 302 4818
ScaI AGT'ACT
Cuts at: 4637 4637
Size: 5120
SexAI A'CCwGG_T
Cuts at: 794 1826 2105 794
Size: 1032 279 3809
Fragments arranged by size:
3809 1032 279
SfcI C'TryA_G
Cuts at: 33 355 439 973 1565 1861 2163 2848 3841
Size: 322 84 534 592 296 302 685 993
Cuts at: 3841 4032 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 592 534 322 302 296
191 84
SfiI GGCCn_nnn'nGGCC
Cuts at: 1865 1865
Size: 5120
SfoI GGC'GCC
Cuts at: 1109 2552 2800 1109
Size: 1443 248 3429
Fragments arranged by size:
3429 1443 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5120
SmaI CCC'GGG
Cuts at: 504 1465 1975 2142 504
Size: 961 510 167 3482
Fragments arranged by size:
3482 961 510 167
SmlI C'TyrA_G
Cuts at: 2275 3190 3682 3944 2275
Size: 915 492 262 3451
Fragments arranged by size:
3451 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5120
SphI G_CATG'C
Cuts at: 2173 2673 2173
Size: 500 4620
StuI AGG'CCT
Cuts at: 271 498 1239 1601 271
Size: 227 741 362 3790
Fragments arranged by size:
3790 741 362 227
StyI C'CwwG_G
Cuts at: 107 339 510 630 1854 2004 2254 2608 107
Size: 232 171 120 1224 150 250 354 2619
Fragments arranged by size:
2619 1224 354 250 232 171 150 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1063 2703 2836 3295 3504 1063
Size: 1640 133 459 209 2679
Fragments arranged by size:
2679 1640 459 209 133
TatI w'GTAC_w
Cuts at: 1059 4635 1059
Size: 3576 1544
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 229 261 1033 1065 1297 1319 1329 1351 2062
Size: 32 772 32 232 22 10 22 711
Cuts at: 2062 2094 4637 4669 229
Size: 32 2543 32 680
Fragments arranged by size:
2543 772 711 680 232 32 32 32
32 22 22 10
Tth111I GACn'n_nGTC
Cuts at: 2917 2917
Size: 5120
XbaI T'CTAG_A
Cuts at: 2151 2151
Size: 5120
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1581 2615 1581
Size: 1034 4086
XmaI C'CCGG_G
Cuts at: 502 1463 1973 2140 502
Size: 961 510 167 3482
Fragments arranged by size:
3482 961 510 167
ZraI GAC'GTC
Cuts at: 1392 2037 1392
Size: 645 4475
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PasI PciI PfoI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI BbsI
BbvCI BcgI BfrBI BmgBI BsiWI BsmI BsmBI BsrGI
BstXI BstZ17I ClaI CspCI FseI FspAI HindIII HpaI
MfeI NdeI NotI NruI NsiI PacI PflMI PmlI
PsiI PspXI PsrI SacII SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI