Restriction map for
pF1KB9148
(Circular) (Six-base) MAPSORT of: pF1KB9148.seq Check: 1295 from: 1 to: 4757
pF1KB9148 4757 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1744 1744
Size: 4757
AccI GT'mk_AC
Cuts at: 777 863 1795 777
Size: 86 932 3739
Fragments arranged by size:
3739 932 86
Acc65I G'GTAC_C
Cuts at: 1773 1773
Size: 4757
AclI AA'CG_TT
Cuts at: 4597 4597
Size: 4757
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2582 3014 3367 4029 2582
Size: 432 353 662 3310
Fragments arranged by size:
3310 662 432 353
AflIII A'CryG_T
Cuts at: 688 2109 3909 4190 688
Size: 1421 1800 281 1255
Fragments arranged by size:
1800 1421 1255 281
AhdI GACnn_n'nnGTC
Cuts at: 266 266
Size: 4757
AlwNI CAG_nnn'CTG
Cuts at: 1183 2062 3500 1183
Size: 879 1438 2440
Fragments arranged by size:
2440 1438 879
ApaLI G'TGCA_C
Cuts at: 520 3595 520
Size: 3075 1682
ApoI r'AATT_y
Cuts at: 1558 1761 4110 4366 1558
Size: 203 2349 256 1949
Fragments arranged by size:
2349 1949 256 203
AseI AT'TA_AT
Cuts at: 20 20
Size: 4757
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4757
AvaI C'yCGr_G
Cuts at: 233 1777 233
Size: 1544 3213
BamHI G'GATC_C
Cuts at: 1782 1782
Size: 4757
BanI G'GyrC_C
Cuts at: 1773 2187 2435 2470 1773
Size: 414 248 35 4060
Fragments arranged by size:
4060 414 248 35
BanII G_rGCy'C
Cuts at: 1163 1771 2801 1163
Size: 608 1030 3119
Fragments arranged by size:
3119 1030 608
BbeI G_GCGC'C
Cuts at: 2191 2439 2191
Size: 248 4509
BbsI GAAGACnn'nnnn_
Cuts at: 857 857
Size: 4757
BbvCI CC'TCA_GC
Cuts at: 1229 1229
Size: 4757
BciVI GTATCCnnnnn_n'
Cuts at: 132 2655 3711 4757 132
Size: 2523 1056 1046 132
Fragments arranged by size:
2523 1056 1046 132
BclI T'GATC_A
Cuts at: 2277 2277
Size: 4757
BglII A'GATC_T
Cuts at: 468 1364 2272 4309 468
Size: 896 908 2037 916
Fragments arranged by size:
2037 916 908 896
BlpI GC'TnA_GC
Cuts at: 274 279 1869 274
Size: 5 1590 3162
Fragments arranged by size:
3162 1590 5
Bme1580I G_kGCm'C
Cuts at: 424 524 2382 2475 3599 424
Size: 100 1858 93 1124 1582
Fragments arranged by size:
1858 1582 1124 100 93
BmrI ACTGGGnnnn_n'
Cuts at: 1969 2050 2385 4529 1969
Size: 81 335 2144 2197
Fragments arranged by size:
2197 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3124 3124
Size: 4757
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1015 1459 1015
Size: 444 4313
Bpu10I CC'TnA_GC
Cuts at: 1229 1229
Size: 4757
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1100 1933 2812 3526 3824 1100
Size: 833 879 714 298 2033
Fragments arranged by size:
2033 879 833 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 253 4461 253
Size: 4208 549
BsaAI yAC'GTr
Cuts at: 954 2110 954
Size: 1156 3601
BsaBI GATnn'nnATC
Cuts at: 6 2271 4308 6
Size: 2265 2037 455
Fragments arranged by size:
2265 2037 455
BsaHI Gr'CG_yC
Cuts at: 2188 2436 3138 4634 2188
Size: 248 702 1496 2311
Fragments arranged by size:
2311 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1385 1946 2467 3145 3556 3703 3977 1385
Size: 561 521 678 411 147 274 2165
Fragments arranged by size:
2165 678 561 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1642 1672 1642
Size: 30 4727
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1074 1660 1663 1074
Size: 586 3 4168
Fragments arranged by size:
4168 586 3
BseYI C'CCAG_C
Cuts at: 2182 2940 3605 4013 2182
Size: 758 665 408 2926
Fragments arranged by size:
2926 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 934 934
Size: 4757
BsiEI CG_ry'CG
Cuts at: 104 2299 2345 3575 104
Size: 2195 46 1230 1286
Fragments arranged by size:
2195 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 524 1309 1712 1727 1771 2549 2739 3599 524
Size: 785 403 15 44 778 190 860 1682
Fragments arranged by size:
1682 860 785 778 403 190 44 15
BsmI GAATG_Cn'
Cuts at: 1298 1298
Size: 4757
Bsp1286I G_dGCh'C
Cuts at: 424 524 1163 1309 1712 1727 1771 2382 2475
Size: 100 639 146 403 15 44 611 93
Cuts at: 2475 2549 2739 2801 3599 424
Size: 74 190 62 798 1582
Fragments arranged by size:
1582 798 639 611 403 190 146 100
93 74 62 44 15
BspHI T'CATG_A
Cuts at: 693 786 3189 693
Size: 93 2403 2261
Fragments arranged by size:
2403 2261 93
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1744 1807 2021 2323 2704 1744
Size: 63 214 302 381 3797
Fragments arranged by size:
3797 381 302 214 63
BsrBI CCG'CTC
Cuts at: 50 3049 3103 4587 4752 50
Size: 2999 54 1484 165 55
Fragments arranged by size:
2999 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 662 2669 662
Size: 2007 2750
BsrFI r'CCGG_y
Cuts at: 2755 2936 2755
Size: 181 4576
BsrGI T'GTAC_A
Cuts at: 506 506
Size: 4757
BssSI C'ACGA_G
Cuts at: 3028 3736 3028
Size: 708 4049
BstAPI GCAn_nnn'nTGC
Cuts at: 725 2134 725
Size: 1409 3348
BstBI TT'CG_AA
Cuts at: 3118 3118
Size: 4757
BstYI r'GATC_y
Cuts at: 1 468 1364 1782 2249 2272 2607 2853 3159
Size: 467 896 418 467 23 335 246 306
Cuts at: 3159 3171 3257 4309 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 896 467 467 449 418 335 306
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 778 778
Size: 4757
BtgI C'CryG_G
Cuts at: 107 240 107
Size: 133 4624
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2707 2888 3051 2707
Size: 181 163 4413
Fragments arranged by size:
4413 181 163
BtsI GCAGTG_nn'
Cuts at: 1633 1662 2000 1633
Size: 29 338 4390
Fragments arranged by size:
4390 338 29
DraI TTT'AAA
Cuts at: 1756 1756
Size: 4757
DrdI GACnn_nn'nnGTC
Cuts at: 859 2463 3807 859
Size: 1604 1344 1809
Fragments arranged by size:
1809 1604 1344
EaeI y'GGCC_r
Cuts at: 826 879 2342 2516 2907 2934 4279 826
Size: 53 1463 174 391 27 1345 1304
Fragments arranged by size:
1463 1345 1304 391 174 53 27
EagI C'GGCC_G
Cuts at: 2342 2342
Size: 4757
EarI CTCTTCn'nnn_
Cuts at: 581 1058 1287 2780 2990 581
Size: 477 229 1493 210 2348
Fragments arranged by size:
2348 1493 477 229 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 151 2965 3707 3853 4567 151
Size: 2814 742 146 714 341
Fragments arranged by size:
2814 742 714 341 146
EcoICRI GAG'CTC
Cuts at: 1769 1769
Size: 4757
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1015 1459 2582 3014 3367 4029 1015
Size: 444 1123 432 353 662 1743
Fragments arranged by size:
1743 1123 662 444 432 353
EcoO109I rG'GnC_Cy
Cuts at: 896 1896 896
Size: 1000 3757
EcoRI G'AATT_C
Cuts at: 1761 1761
Size: 4757
EcoRV GAT'ATC
Cuts at: 927 927
Size: 4757
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 313 744 1298 2776 313
Size: 431 554 1478 2294
Fragments arranged by size:
2294 1478 554 431
FspI TGC'GCA
Cuts at: 2538 2538
Size: 4757
HaeII r_GCGC'y
Cuts at: 2191 2439 3669 4440 2191
Size: 248 1230 771 2508
Fragments arranged by size:
2508 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 252 284 425 457 1057 1089 2599 2629 2631
Size: 32 141 32 600 32 1510 30 2
Cuts at: 2631 2661 252
Size: 30 2348
Fragments arranged by size:
2348 1510 600 141 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 864 936 1256 1796 864
Size: 72 320 540 3825
Fragments arranged by size:
3825 540 320 72
KasI G'GCGC_C
Cuts at: 2187 2435 2187
Size: 248 4509
KpnI G_GTAC'C
Cuts at: 1777 1777
Size: 4757
MfeI C'AATT_G
Cuts at: 607 607
Size: 4757
MluI A'CGCG_T
Cuts at: 4190 4190
Size: 4757
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 952 1376 3516 3700 952
Size: 424 2140 184 2009
Fragments arranged by size:
2140 2009 424 184
MscI TGG'CCA
Cuts at: 828 881 2518 828
Size: 53 1637 3067
Fragments arranged by size:
3067 1637 53
MslI CAynn'nnrTG
Cuts at: 2746 2873 2746
Size: 127 4630
MspA1I CmG'CkG
Cuts at: 273 529 1868 2182 2542 3326 3571 273
Size: 256 1339 314 360 784 245 1459
Fragments arranged by size:
1459 1339 784 360 314 256 245
NaeI GCC'GGC
Cuts at: 2938 2938
Size: 4757
NarI GG'CG_CC
Cuts at: 2188 2436 2188
Size: 248 4509
NcoI C'CATG_G
Cuts at: 107 107
Size: 4757
NgoMIV G'CCGG_C
Cuts at: 2936 2936
Size: 4757
NspI r_CATG'y
Cuts at: 692 1344 1506 1810 2310 3913 692
Size: 652 162 304 500 1603 1536
Fragments arranged by size:
1603 1536 652 500 304 162
PciI A'CATG_T
Cuts at: 688 3909 688
Size: 3221 1536
PmeI GTTT'AAAC
Cuts at: 1756 1756
Size: 4757
PmlI CAC'GTG
Cuts at: 954 954
Size: 4757
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3203 3235 3203
Size: 32 4725
PshAI GACnn'nnGTC
Cuts at: 689 689
Size: 4757
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1666 1698 1666
Size: 32 4725
PstI C_TGCA'G
Cuts at: 713 1804 2489 713
Size: 1091 685 2981
Fragments arranged by size:
2981 1091 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4757
PvuII CAG'CTG
Cuts at: 273 529 2182 2542 273
Size: 256 1653 360 2488
Fragments arranged by size:
2488 1653 360 256
RsrII CG'GwC_CG
Cuts at: 2952 2952
Size: 4757
SacI G_AGCT'C
Cuts at: 1771 1771
Size: 4757
SalI G'TCGA_C
Cuts at: 862 1794 862
Size: 932 3825
SapI GCTCTTCn'nnn_
Cuts at: 2780 2990 2780
Size: 210 4547
SbfI CC_TGCA'GG
Cuts at: 1804 1804
Size: 4757
ScaI AGT'ACT
Cuts at: 4274 4274
Size: 4757
SfcI C'TryA_G
Cuts at: 33 464 709 1493 1800 2485 3453 3644 33
Size: 431 245 784 307 685 968 191 1146
Fragments arranged by size:
1146 968 784 685 431 307 245 191
SfoI GGC'GCC
Cuts at: 2189 2437 2189
Size: 248 4509
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4757
SmaI CCC'GGG
Cuts at: 1779 1779
Size: 4757
SmlI C'TyrA_G
Cuts at: 233 1115 1912 2827 3541 3803 233
Size: 882 797 915 714 262 1187
Fragments arranged by size:
1187 915 882 797 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4757
SphI G_CATG'C
Cuts at: 1810 2310 1810
Size: 500 4257
StuI AGG'CCT
Cuts at: 982 982
Size: 4757
StyI C'CwwG_G
Cuts at: 107 1891 2245 107
Size: 1784 354 2619
Fragments arranged by size:
2619 1784 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1067 1324 2340 2473 2932 3141 1067
Size: 257 1016 133 459 209 2683
Fragments arranged by size:
2683 1016 459 257 209 133
TatI w'GTAC_w
Cuts at: 506 4272 506
Size: 3766 991
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4274 4306 4274
Size: 32 4725
Tth111I GACn'n_nGTC
Cuts at: 2554 2554
Size: 4757
XbaI T'CTAG_A
Cuts at: 1788 1788
Size: 4757
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2252 2252
Size: 4757
XhoI C'TCGA_G
Cuts at: 233 233
Size: 4757
XmaI C'CCGG_G
Cuts at: 1777 1777
Size: 4757
XmnI GAAnn'nnTTC
Cuts at: 917 917
Size: 4757
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BstZ17I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PmlI
PpiI PshAI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsiWI BsmBI BspEI BssHII BstEII BstXI Bsu36I ClaI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PpuMI PsiI PspOMI PspXI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI ZraI