Restriction map for
pF1KB9134
(Circular) (Six-base) MAPSORT of: pF1KB9134.seq Check: 9995 from: 1 to: 7049
pF1KB9134 7049 bp
With 182 enzymes: SgfI *
July 27, 2015 09:05 ..
AccI GT'mk_AC
Cuts at: 1076 1164 4087 1076
Size: 88 2923 4038
Fragments arranged by size:
4038 2923 88
Acc65I G'GTAC_C
Cuts at: 4065 4065
Size: 7049
AclI AA'CG_TT
Cuts at: 3766 6889 3766
Size: 3123 3926
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 766 1502 2468 2716 2816 3694 4874 5306
Size: 592 736 966 248 100 878 1180 432
Cuts at: 5306 6053 6321 174
Size: 747 268 902
Fragments arranged by size:
1180 966 902 878 747 736 592 432
268 248 100
AflIII A'CryG_T
Cuts at: 1708 4401 5505 6482 1708
Size: 2693 1104 977 2275
Fragments arranged by size:
2693 2275 1104 977
AgeI A'CCGG_T
Cuts at: 5437 5437
Size: 7049
AhdI GACnn_n'nnGTC
Cuts at: 704 704
Size: 7049
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4022 4054 4022
Size: 32 7017
AlwNI CAG_nnn'CTG
Cuts at: 2182 2766 3634 3900 4354 5921 2182
Size: 584 868 266 454 1567 3310
Fragments arranged by size:
3310 1567 868 584 454 266
ApaLI G'TGCA_C
Cuts at: 5819 5819
Size: 7049
ApoI r'AATT_y
Cuts at: 436 1290 3508 3961 4053 6402 6658 436
Size: 854 2218 453 92 2349 256 827
Fragments arranged by size:
2349 2218 854 827 453 256 92
AseI AT'TA_AT
Cuts at: 20 1216 1868 2893 20
Size: 1196 652 1025 4176
Fragments arranged by size:
4176 1196 1025 652
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7049
AvaI C'yCGr_G
Cuts at: 4069 4069
Size: 7049
BamHI G'GATC_C
Cuts at: 4074 4074
Size: 7049
BanI G'GyrC_C
Cuts at: 4065 4479 4727 4762 4065
Size: 414 248 35 6352
Fragments arranged by size:
6352 414 248 35
BanII G_rGCy'C
Cuts at: 348 499 1550 4063 5093 348
Size: 151 1051 2513 1030 2304
Fragments arranged by size:
2513 2304 1051 1030 151
BbeI G_GCGC'C
Cuts at: 4483 4731 4483
Size: 248 6801
BbsI GAAGACnn'nnnn_
Cuts at: 1844 1991 1844
Size: 147 6902
BbvCI CC'TCA_GC
Cuts at: 3407 3407
Size: 7049
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1162 1196 3689 3723 1162
Size: 34 2493 34 4488
Fragments arranged by size:
4488 2493 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4947 5708 7049 4947
Size: 761 1341 4947
Fragments arranged by size:
4947 1341 761
BclI T'GATC_A
Cuts at: 527 780 4569 527
Size: 253 3789 3007
Fragments arranged by size:
3789 3007 253
BglI GCCn_nnn'nGGC
Cuts at: 2083 2313 2083
Size: 230 6819
BglII A'GATC_T
Cuts at: 400 4564 6601 400
Size: 4164 2037 848
Fragments arranged by size:
4164 2037 848
BlpI GC'TnA_GC
Cuts at: 2549 4161 2549
Size: 1612 5437
Bme1580I G_kGCm'C
Cuts at: 2309 4674 4767 5823 2309
Size: 2365 93 1056 3535
Fragments arranged by size:
3535 2365 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 798 1432 2103 4261 4342 4677 6821 798
Size: 634 671 2158 81 335 2144 1026
Fragments arranged by size:
2158 2144 1026 671 634 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5416 5416
Size: 7049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 2108 2216 2788 290
Size: 1818 108 572 4551
Fragments arranged by size:
4551 1818 572 108
Bpu10I CC'TnA_GC
Cuts at: 3407 3407
Size: 7049
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 2227 4225 5104 5596 5894 311
Size: 1916 1998 879 492 298 1466
Fragments arranged by size:
1998 1916 1466 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6753 6753
Size: 7049
BsaAI yAC'GTr
Cuts at: 1711 3550 4402 1711
Size: 1839 852 4358
Fragments arranged by size:
4358 1839 852
BsaBI GATnn'nnATC
Cuts at: 6 1184 4563 6600 6
Size: 1178 3379 2037 455
Fragments arranged by size:
3379 2037 1178 455
BsaHI Gr'CG_yC
Cuts at: 4480 4728 5430 6926 4480
Size: 248 702 1496 4603
Fragments arranged by size:
4603 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4238 4759 5437 5711 5858 6269 4238
Size: 521 678 274 147 411 5018
Fragments arranged by size:
5018 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2785 2785
Size: 7049
BseYI C'CCAG_C
Cuts at: 2295 4474 5232 5809 6305 2295
Size: 2179 758 577 496 3039
Fragments arranged by size:
3039 2179 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 527 527
Size: 7049
BsiEI CG_ry'CG
Cuts at: 104 4591 4637 5845 104
Size: 4487 46 1208 1308
Fragments arranged by size:
4487 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 499 1550 3997 4063 4841 5031 5823 499
Size: 1051 2447 66 778 190 792 1725
Fragments arranged by size:
2447 1725 1051 792 778 190 66
BsmI GAATG_Cn'
Cuts at: 131 3079 131
Size: 2948 4101
Bsp1286I G_dGCh'C
Cuts at: 348 499 1550 2309 3997 4063 4674 4767 4841
Size: 151 1051 759 1688 66 611 93 74
Cuts at: 4841 5031 5093 5823 348
Size: 190 62 730 1574
Fragments arranged by size:
1688 1574 1051 759 730 611 190 151
93 74 66 62
BspEI T'CCGG_A
Cuts at: 6269 6269
Size: 7049
BspHI T'CATG_A
Cuts at: 3116 3912 6225 3116
Size: 796 2313 3940
Fragments arranged by size:
3940 2313 796
BspMI ACCTGCnnnn'nnnn_
Cuts at: 678 2829 3243 4099 4313 4615 4996 678
Size: 2151 414 856 214 302 381 2731
Fragments arranged by size:
2731 2151 856 414 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5341 5395 6879 7044 50
Size: 5291 54 1484 165 55
Fragments arranged by size:
5291 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1599 3788 4961 1599
Size: 2189 1173 3687
Fragments arranged by size:
3687 2189 1173
BsrFI r'CCGG_y
Cuts at: 5047 5228 5437 5047
Size: 181 209 6659
Fragments arranged by size:
6659 209 181
BsrGI T'GTAC_A
Cuts at: 306 2810 306
Size: 2504 4545
BssSI C'ACGA_G
Cuts at: 5320 5678 5320
Size: 358 6691
BstAPI GCAn_nnn'nTGC
Cuts at: 1336 3258 4426 1336
Size: 1922 1168 3959
Fragments arranged by size:
3959 1922 1168
BstBI TT'CG_AA
Cuts at: 5410 5410
Size: 7049
BstYI r'GATC_y
Cuts at: 1 400 1513 3465 4074 4541 4564 4899 5145
Size: 399 1113 1952 609 467 23 335 246
Cuts at: 5145 6157 6243 6255 6601 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1952 1113 1012 609 467 449 399 346
335 246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4999 5180 5343 4999
Size: 181 163 6705
Fragments arranged by size:
6705 181 163
BtsI GCAGTG_nn'
Cuts at: 3717 4020 4292 3717
Size: 303 272 6474
Fragments arranged by size:
6474 303 272
ClaI AT'CG_AT
Cuts at: 3986 3986
Size: 7049
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3136 3171 3136
Size: 35 7014
DraI TTT'AAA
Cuts at: 441 4048 441
Size: 3607 3442
DrdI GACnn_nn'nnGTC
Cuts at: 4755 5613 4755
Size: 858 6191
EaeI y'GGCC_r
Cuts at: 4634 4808 5199 5226 6571 4634
Size: 174 391 27 1345 5112
Fragments arranged by size:
5112 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4634 4634
Size: 7049
EarI CTCTTCn'nnn_
Cuts at: 971 1958 3284 5072 5282 971
Size: 987 1326 1788 210 2738
Fragments arranged by size:
2738 1788 1326 987 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3660 5257 5567 5713 6859 3660
Size: 1597 310 146 1146 3850
Fragments arranged by size:
3850 1597 1146 310 146
EcoICRI GAG'CTC
Cuts at: 497 1548 4061 497
Size: 1051 2513 3485
Fragments arranged by size:
3485 2513 1051
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 290 766 1502 2108 2216 2468 2716 2788
Size: 116 476 736 606 108 252 248 72
Cuts at: 2788 2816 3694 4874 5306 6053 6321 174
Size: 28 878 1180 432 747 268 902
Fragments arranged by size:
1180 902 878 747 736 606 476 432
268 252 248 116 108 72 28
EcoO109I rG'GnC_Cy
Cuts at: 1655 4188 1655
Size: 2533 4516
EcoRI G'AATT_C
Cuts at: 4053 4053
Size: 7049
EcoRV GAT'ATC
Cuts at: 2738 2738
Size: 7049
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3187 5068 3187
Size: 1881 5168
FspI TGC'GCA
Cuts at: 4830 4830
Size: 7049
HaeII r_GCGC'y
Cuts at: 2397 4483 4731 5753 6732 2397
Size: 2086 248 1022 979 2714
Fragments arranged by size:
2714 2086 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 512 519 544 551 690 722 765 797 1429
Size: 7 25 7 139 32 43 32 632
Cuts at: 1429 1461 1957 1989 2197 2229 2676 2704 2708
Size: 32 496 32 208 32 447 28 4
Cuts at: 2708 2736 3979 4011 4891 4921 4923 4953 512
Size: 28 1243 32 880 30 2 30 2608
Fragments arranged by size:
2608 1243 880 632 496 447 208 139
43 32 32 32 32 32 32 30
30 28 28 25 7 7 4 2
HincII GTy'rAC
Cuts at: 809 2249 2592 4088 809
Size: 1440 343 1496 3770
Fragments arranged by size:
3770 1496 1440 343
HindIII A'AGCT_T
Cuts at: 764 2659 764
Size: 1895 5154
HpaI GTT'AAC
Cuts at: 809 809
Size: 7049
KasI G'GCGC_C
Cuts at: 4479 4727 4479
Size: 248 6801
KpnI G_GTAC'C
Cuts at: 4069 4069
Size: 7049
MluI A'CGCG_T
Cuts at: 6482 6482
Size: 7049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 192 1487 2607 3266 5720 5904 192
Size: 1295 1120 659 2454 184 1337
Fragments arranged by size:
2454 1337 1295 1120 659 184
MscI TGG'CCA
Cuts at: 4810 4810
Size: 7049
MslI CAynn'nnrTG
Cuts at: 1787 5038 5165 1787
Size: 3251 127 3671
Fragments arranged by size:
3671 3251 127
MspA1I CmG'CkG
Cuts at: 1170 4160 4474 4834 5847 6092 1170
Size: 2990 314 360 1013 245 2127
Fragments arranged by size:
2990 2127 1013 360 314 245
NaeI GCC'GGC
Cuts at: 5230 5230
Size: 7049
NarI GG'CG_CC
Cuts at: 4480 4728 4480
Size: 248 6801
NgoMIV G'CCGG_C
Cuts at: 5228 5228
Size: 7049
NspI r_CATG'y
Cuts at: 3850 4102 4602 5509 3850
Size: 252 500 907 5390
Fragments arranged by size:
5390 907 500 252
PasI CC'CwG_GG
Cuts at: 4026 4026
Size: 7049
PciI A'CATG_T
Cuts at: 5505 5505
Size: 7049
PflMI CCAn_nnn'nTGG
Cuts at: 1680 3900 1680
Size: 2220 4829
PfoI T'CCnGG_A
Cuts at: 112 282 112
Size: 170 6879
PmeI GTTT'AAAC
Cuts at: 4048 4048
Size: 7049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 875 907 6188 6220 875
Size: 32 5281 32 1704
Fragments arranged by size:
5281 1704 32 32
PpuMI rG'GwC_Cy
Cuts at: 1655 1655
Size: 7049
PsiI TTA'TAA
Cuts at: 3482 3653 3482
Size: 171 6878
PstI C_TGCA'G
Cuts at: 2372 4096 4781 2372
Size: 1724 685 4640
Fragments arranged by size:
4640 1724 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7049
PvuII CAG'CTG
Cuts at: 1170 4474 4834 1170
Size: 3304 360 3385
Fragments arranged by size:
3385 3304 360
RsrII CG'GwC_CG
Cuts at: 5244 5244
Size: 7049
SacI G_AGCT'C
Cuts at: 499 1550 4063 499
Size: 1051 2513 3485
Fragments arranged by size:
3485 2513 1051
SalI G'TCGA_C
Cuts at: 4086 4086
Size: 7049
SapI GCTCTTCn'nnn_
Cuts at: 1958 5072 5282 1958
Size: 3114 210 3725
Fragments arranged by size:
3725 3114 210
SbfI CC_TGCA'GG
Cuts at: 4096 4096
Size: 7049
ScaI AGT'ACT
Cuts at: 6566 6566
Size: 7049
SfcI C'TryA_G
Cuts at: 33 1165 2368 3966 4092 4777 5770 5961 33
Size: 1132 1203 1598 126 685 993 191 1121
Fragments arranged by size:
1598 1203 1132 1121 993 685 191 126
SfoI GGC'GCC
Cuts at: 4481 4729 4481
Size: 248 6801
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7049
SmaI CCC'GGG
Cuts at: 4071 4071
Size: 7049
SmlI C'TyrA_G
Cuts at: 326 2242 4204 5119 5611 5873 326
Size: 1916 1962 915 492 262 1502
Fragments arranged by size:
1962 1916 1502 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7049
SphI G_CATG'C
Cuts at: 4102 4602 4102
Size: 500 6549
SspI AAT'ATT
Cuts at: 2967 2967
Size: 7049
StyI C'CwwG_G
Cuts at: 317 548 929 3689 3794 4183 4537 317
Size: 231 381 2760 105 389 354 2829
Fragments arranged by size:
2829 2760 389 381 354 231 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 533 3836 4632 4765 5224 5433 533
Size: 3303 796 133 459 209 2149
Fragments arranged by size:
3303 2149 796 459 209 133
TatI w'GTAC_w
Cuts at: 265 306 412 2701 2810 6564 265
Size: 41 106 2289 109 3754 750
Fragments arranged by size:
3754 2289 750 109 106 41
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6566 6598 6566
Size: 32 7017
Tth111I GACn'n_nGTC
Cuts at: 4846 4846
Size: 7049
XbaI T'CTAG_A
Cuts at: 4080 4080
Size: 7049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4544 4544
Size: 7049
XmaI C'CCGG_G
Cuts at: 4069 4069
Size: 7049
XmnI GAAnn'nnTTC
Cuts at: 680 2684 3362 680
Size: 2004 678 4367
Fragments arranged by size:
4367 2004 678
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI ApaI AscI
AvrII BaeI BfrBI BmgBI BmtI BsaXI BsiWI BsmBI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI DraIII EcoNI
FseI FspAI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI