Restriction map for
pF1KB9128
(Circular) (Six-base) MAPSORT of: pF1KB9128.seq Check: 8363 from: 1 to: 5819
pF1KB9128 5819 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 1582 2857 1582
Size: 1275 4544
Acc65I G'GTAC_C
Cuts at: 1296 2835 1296
Size: 1539 4280
AclI AA'CG_TT
Cuts at: 223 5659 223
Size: 5436 383
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 707 2065 2530 2726 3644 4076 4823 5091
Size: 192 1358 465 196 918 432 747 268
Cuts at: 5091 515
Size: 1243
Fragments arranged by size:
1358 1243 918 747 465 432 268 196
192
AflII C'TTAA_G
Cuts at: 1177 1177
Size: 5819
AflIII A'CryG_T
Cuts at: 3171 4275 5252 3171
Size: 1104 977 3738
Fragments arranged by size:
3738 1104 977
AgeI A'CCGG_T
Cuts at: 4207 4207
Size: 5819
AleI CACnn'nnGTG
Cuts at: 2620 2620
Size: 5819
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2090 2090
Size: 5819
AlwNI CAG_nnn'CTG
Cuts at: 642 3124 4691 642
Size: 2482 1567 1770
Fragments arranged by size:
2482 1770 1567
ApaLI G'TGCA_C
Cuts at: 4589 4589
Size: 5819
ApoI r'AATT_y
Cuts at: 288 389 461 694 789 1092 1150 1311 1699
Size: 101 72 233 95 303 58 161 388
Cuts at: 1699 2823 5172 5428 288
Size: 1124 2349 256 679
Fragments arranged by size:
2349 1124 679 388 303 256 233 161
101 95 72 58
AseI AT'TA_AT
Cuts at: 20 557 20
Size: 537 5282
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5819
AvaI C'yCGr_G
Cuts at: 2839 2839
Size: 5819
BamHI G'GATC_C
Cuts at: 2844 2844
Size: 5819
BanI G'GyrC_C
Cuts at: 1296 1449 2835 3249 3497 3532 1296
Size: 153 1386 414 248 35 3583
Fragments arranged by size:
3583 1386 414 248 153 35
BanII G_rGCy'C
Cuts at: 2833 3863 2833
Size: 1030 4789
BbeI G_GCGC'C
Cuts at: 3253 3501 3253
Size: 248 5571
BbsI GAAGACnn'nnnn_
Cuts at: 1012 1198 1414 1012
Size: 186 216 5417
Fragments arranged by size:
5417 216 186
BciVI GTATCCnnnnn_n'
Cuts at: 938 1357 3717 4478 5819 938
Size: 419 2360 761 1341 938
Fragments arranged by size:
2360 1341 938 761 419
BclI T'GATC_A
Cuts at: 3339 3339
Size: 5819
BfrBI ATG'CAT
Cuts at: 972 2067 972
Size: 1095 4724
BglII A'GATC_T
Cuts at: 3334 5371 3334
Size: 2037 3782
BlpI GC'TnA_GC
Cuts at: 2931 2931
Size: 5819
Bme1580I G_kGCm'C
Cuts at: 2156 3444 3537 4593 2156
Size: 1288 93 1056 3382
Fragments arranged by size:
3382 1288 1056 93
BmgBI CAC'GTC
Cuts at: 2435 2435
Size: 5819
BmrI ACTGGGnnnn_n'
Cuts at: 810 2119 3031 3112 3447 5591 810
Size: 1309 912 81 335 2144 1038
Fragments arranged by size:
2144 1309 1038 912 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4186 4186
Size: 5819
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1473 1733 1473
Size: 260 5559
Bpu10I CC'TnA_GC
Cuts at: 1172 1172
Size: 5819
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 731 1189 1740 2995 3874 4366 4664 557
Size: 174 458 551 1255 879 492 298 1712
Fragments arranged by size:
1712 1255 879 551 492 458 298 174
BsaI GGTCTCn'nnnn_
Cuts at: 2778 5523 2778
Size: 2745 3074
BsaAI yAC'GTr
Cuts at: 1533 3172 1533
Size: 1639 4180
BsaBI GATnn'nnATC
Cuts at: 6 3333 5370 6
Size: 3327 2037 455
Fragments arranged by size:
3327 2037 455
BsaHI Gr'CG_yC
Cuts at: 3250 3498 4200 5696 3250
Size: 248 702 1496 3373
Fragments arranged by size:
3373 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3008 3529 4207 4481 4628 5039 3008
Size: 521 678 274 147 411 3788
Fragments arranged by size:
3788 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 3244 4002 4579 5075 3244
Size: 758 577 496 3988
Fragments arranged by size:
3988 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3361 3407 4615 104
Size: 3257 46 1208 1308
Fragments arranged by size:
3257 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1743 2833 3611 3801 4593 1743
Size: 1090 778 190 792 2969
Fragments arranged by size:
2969 1090 792 778 190
BsmI GAATG_Cn'
Cuts at: 159 1864 159
Size: 1705 4114
Bsp1286I G_dGCh'C
Cuts at: 1743 2156 2833 3444 3537 3611 3801 3863 4593
Size: 413 677 611 93 74 190 62 730
Cuts at: 4593 1743
Size: 2969
Fragments arranged by size:
2969 730 677 611 413 190 93 74
62
BspEI T'CCGG_A
Cuts at: 5039 5039
Size: 5819
BspHI T'CATG_A
Cuts at: 2780 4995 2780
Size: 2215 3604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2721 2869 3083 3385 3766 2721
Size: 148 214 302 381 4774
Fragments arranged by size:
4774 381 302 214 148
BsrBI CCG'CTC
Cuts at: 50 1606 4111 4165 5649 5814 50
Size: 1556 2505 54 1484 165 55
Fragments arranged by size:
2505 1556 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 897 2036 3731 897
Size: 1139 1695 2985
Fragments arranged by size:
2985 1695 1139
BsrFI r'CCGG_y
Cuts at: 3817 3998 4207 3817
Size: 181 209 5429
Fragments arranged by size:
5429 209 181
BssSI C'ACGA_G
Cuts at: 4090 4448 4090
Size: 358 5461
BstAPI GCAn_nnn'nTGC
Cuts at: 403 642 2026 3196 403
Size: 239 1384 1170 3026
Fragments arranged by size:
3026 1384 1170 239
BstBI TT'CG_AA
Cuts at: 1221 4180 1221
Size: 2959 2860
BstXI CCAn_nnnn'nTGG
Cuts at: 1246 2619 1246
Size: 1373 4446
BstYI r'GATC_y
Cuts at: 1 1999 2844 3311 3334 3669 3915 4927 5013
Size: 1998 845 467 23 335 246 1012 86
Cuts at: 5013 5025 5371 1
Size: 12 346 449
Fragments arranged by size:
1998 1012 845 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1583 1583
Size: 5819
BtgI C'CryG_G
Cuts at: 107 107
Size: 5819
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3769 3950 4113 3769
Size: 181 163 5475
Fragments arranged by size:
5475 181 163
BtsI GCAGTG_nn'
Cuts at: 1138 3062 1138
Size: 1924 3895
DraI TTT'AAA
Cuts at: 1523 2301 2818 1523
Size: 778 517 4524
Fragments arranged by size:
4524 778 517
DrdI GACnn_nn'nnGTC
Cuts at: 521 3525 4383 521
Size: 3004 858 1957
Fragments arranged by size:
3004 1957 858
EaeI y'GGCC_r
Cuts at: 3404 3578 3969 3996 5341 3404
Size: 174 391 27 1345 3882
Fragments arranged by size:
3882 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3404 3404
Size: 5819
EarI CTCTTCn'nnn_
Cuts at: 1462 2549 3842 4052 1462
Size: 1087 1293 210 3229
Fragments arranged by size:
3229 1293 1087 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4027 4337 4483 5629 4027
Size: 310 146 1146 4217
Fragments arranged by size:
4217 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2831 2831
Size: 5819
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 707 1473 1733 2065 2530 2726 3644 4076
Size: 192 766 260 332 465 196 918 432
Cuts at: 4076 4823 5091 515
Size: 747 268 1243
Fragments arranged by size:
1243 918 766 747 465 432 332 268
260 196 192
EcoNI CCTnn'n_nnAGG
Cuts at: 985 985
Size: 5819
EcoO109I rG'GnC_Cy
Cuts at: 959 1762 2774 2958 959
Size: 803 1012 184 3820
Fragments arranged by size:
3820 1012 803 184
EcoRI G'AATT_C
Cuts at: 1699 2823 1699
Size: 1124 4695
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3838 3838
Size: 5819
FspI TGC'GCA
Cuts at: 1863 3600 1863
Size: 1737 4082
FspAI rTGC'GCAy
Cuts at: 1863 1863
Size: 5819
HaeII r_GCGC'y
Cuts at: 3253 3501 4523 5502 3253
Size: 248 1022 979 3570
Fragments arranged by size:
3570 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 361 393 729 761 790 822 1407 1439 1602
Size: 32 336 32 29 32 585 32 163
Cuts at: 1602 1617 1634 1649 3661 3691 3693 3723 361
Size: 15 17 15 2012 30 2 30 2457
Fragments arranged by size:
2457 2012 585 336 163 32 32 32
32 30 30 29 17 15 15 2
HincII GTy'rAC
Cuts at: 2858 2858
Size: 5819
HindIII A'AGCT_T
Cuts at: 841 1207 841
Size: 366 5453
KasI G'GCGC_C
Cuts at: 3249 3497 3249
Size: 248 5571
KpnI G_GTAC'C
Cuts at: 1300 2839 1300
Size: 1539 4280
MfeI C'AATT_G
Cuts at: 2639 2639
Size: 5819
MluI A'CGCG_T
Cuts at: 5252 5252
Size: 5819
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1341 2290 2307 2774 4490 4674 1341
Size: 949 17 467 1716 184 2486
Fragments arranged by size:
2486 1716 949 467 184 17
MscI TGG'CCA
Cuts at: 3580 3580
Size: 5819
MslI CAynn'nnrTG
Cuts at: 860 2620 3808 3935 860
Size: 1760 1188 127 2744
Fragments arranged by size:
2744 1760 1188 127
MspA1I CmG'CkG
Cuts at: 442 2930 3244 3604 4617 4862 442
Size: 2488 314 360 1013 245 1399
Fragments arranged by size:
2488 1399 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4000 4000
Size: 5819
NarI GG'CG_CC
Cuts at: 3250 3498 3250
Size: 248 5571
NcoI C'CATG_G
Cuts at: 107 107
Size: 5819
NgoMIV G'CCGG_C
Cuts at: 3998 3998
Size: 5819
NsiI A_TGCA'T
Cuts at: 974 2069 974
Size: 1095 4724
NspI r_CATG'y
Cuts at: 1276 1385 2872 3372 4279 1276
Size: 109 1487 500 907 2816
Fragments arranged by size:
2816 1487 907 500 109
PasI CC'CwG_GG
Cuts at: 169 169
Size: 5819
PciI A'CATG_T
Cuts at: 4275 4275
Size: 5819
PflMI CCAn_nnn'nTGG
Cuts at: 1293 1293
Size: 5819
PfoI T'CCnGG_A
Cuts at: 1772 1871 1772
Size: 99 5720
PmeI GTTT'AAAC
Cuts at: 2818 2818
Size: 5819
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1553 1585 4958 4990 1553
Size: 32 3373 32 2382
Fragments arranged by size:
3373 2382 32 32
PpuMI rG'GwC_Cy
Cuts at: 959 1762 959
Size: 803 5016
PshAI GACnn'nnGTC
Cuts at: 2790 2790
Size: 5819
PsiI TTA'TAA
Cuts at: 1120 2581 1120
Size: 1461 4358
PstI C_TGCA'G
Cuts at: 1145 2697 2866 3551 1145
Size: 1552 169 685 3413
Fragments arranged by size:
3413 1552 685 169
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5819
PvuII CAG'CTG
Cuts at: 442 3244 3604 442
Size: 2802 360 2657
Fragments arranged by size:
2802 2657 360
RsrII CG'GwC_CG
Cuts at: 4014 4014
Size: 5819
SacI G_AGCT'C
Cuts at: 2833 2833
Size: 5819
SalI G'TCGA_C
Cuts at: 2856 2856
Size: 5819
SapI GCTCTTCn'nnn_
Cuts at: 3842 4052 3842
Size: 210 5609
SbfI CC_TGCA'GG
Cuts at: 2866 2866
Size: 5819
ScaI AGT'ACT
Cuts at: 2586 5336 2586
Size: 2750 3069
SfcI C'TryA_G
Cuts at: 33 281 740 982 1141 2693 2862 3547 4540
Size: 248 459 242 159 1552 169 685 993
Cuts at: 4540 4731 33
Size: 191 1121
Fragments arranged by size:
1552 1121 993 685 459 248 242 191
169 159
SfoI GGC'GCC
Cuts at: 3251 3499 3251
Size: 248 5571
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5819
SmaI CCC'GGG
Cuts at: 2841 2841
Size: 5819
SmlI C'TyrA_G
Cuts at: 572 746 1177 1204 1719 2974 3889 4381 4643
Size: 174 431 27 515 1255 915 492 262
Cuts at: 4643 572
Size: 1748
Fragments arranged by size:
1748 1255 915 515 492 431 262 174
27
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5819
SphI G_CATG'C
Cuts at: 2872 3372 2872
Size: 500 5319
SspI AAT'ATT
Cuts at: 1163 1810 2457 1163
Size: 647 647 4525
Fragments arranged by size:
4525 647 647
StyI C'CwwG_G
Cuts at: 107 2953 3307 107
Size: 2846 354 2619
Fragments arranged by size:
2846 2619 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3402 3535 3994 4203 3402
Size: 133 459 209 5018
Fragments arranged by size:
5018 459 209 133
TatI w'GTAC_w
Cuts at: 576 2584 5334 576
Size: 2008 2750 1061
Fragments arranged by size:
2750 2008 1061
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5336 5368 5336
Size: 32 5787
Tth111I GACn'n_nGTC
Cuts at: 3616 3616
Size: 5819
XbaI T'CTAG_A
Cuts at: 2850 2850
Size: 5819
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2619 3314 2619
Size: 695 5124
XmaI C'CCGG_G
Cuts at: 2839 2839
Size: 5819
XmnI GAAnn'nnTTC
Cuts at: 389 1019 1094 1217 389
Size: 630 75 123 4991
Fragments arranged by size:
4991 630 123 75
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BglI BmtI BsaXI BseRI BsgI
BsiWI BsmBI BsrGI BssHII BstEII Bsu36I ClaI CspCI
DraIII EcoRV FseI HpaI NdeI NheI NotI NruI
PacI PmlI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI