Restriction map for
pF1KB9127
(Circular) (Six-base) MAPSORT of: pF1KB9127.seq Check: 2357 from: 1 to: 5570
pF1KB9127 5570 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 2608 2608
Size: 5570
Acc65I G'GTAC_C
Cuts at: 2586 2586
Size: 5570
AclI AA'CG_TT
Cuts at: 1972 2308 5410 1972
Size: 336 3102 2132
Fragments arranged by size:
3102 2132 336
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3395 3827 4180 4842 3395
Size: 432 353 662 4123
Fragments arranged by size:
4123 662 432 353
AflIII A'CryG_T
Cuts at: 2922 4722 5003 2922
Size: 1800 281 3489
Fragments arranged by size:
3489 1800 281
AleI CACnn'nnGTG
Cuts at: 1010 1070 1178 1910 2330 1010
Size: 60 108 732 420 4250
Fragments arranged by size:
4250 732 420 108 60
AlwNI CAG_nnn'CTG
Cuts at: 1205 2875 4313 1205
Size: 1670 1438 2462
Fragments arranged by size:
2462 1670 1438
ApaLI G'TGCA_C
Cuts at: 1913 2165 2333 4408 1913
Size: 252 168 2075 3075
Fragments arranged by size:
3075 2075 252 168
ApoI r'AATT_y
Cuts at: 114 372 427 494 523 741 767 917 1001
Size: 258 55 67 29 218 26 150 84
Cuts at: 1001 1169 1253 1337 1421 1505 1673 1757 1841
Size: 168 84 84 84 84 168 84 84
Cuts at: 1841 1925 2009 2261 2345 2403 2429 2574 4923
Size: 84 84 252 84 58 26 145 2349
Cuts at: 4923 5179 114
Size: 256 505
Fragments arranged by size:
2349 505 258 256 252 218 168 168
150 145 84 84 84 84 84 84
84 84 84 84 67 58 55 29
26 26
AseI AT'TA_AT
Cuts at: 20 287 20
Size: 267 5303
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5570
AvaI C'yCGr_G
Cuts at: 2590 2590
Size: 5570
BamHI G'GATC_C
Cuts at: 2595 2595
Size: 5570
BanI G'GyrC_C
Cuts at: 2586 3000 3248 3283 2586
Size: 414 248 35 4873
Fragments arranged by size:
4873 414 248 35
BanII G_rGCy'C
Cuts at: 388 2584 3614 388
Size: 2196 1030 2344
Fragments arranged by size:
2344 2196 1030
BbeI G_GCGC'C
Cuts at: 3004 3252 3004
Size: 248 5322
BbsI GAAGACnn'nnnn_
Cuts at: 895 1654 2059 895
Size: 759 405 4406
Fragments arranged by size:
4406 759 405
BciVI GTATCCnnnnn_n'
Cuts at: 3468 4524 5570 3468
Size: 1056 1046 3468
Fragments arranged by size:
3468 1056 1046
BclI T'GATC_A
Cuts at: 3090 3090
Size: 5570
BfrBI ATG'CAT
Cuts at: 2520 2520
Size: 5570
BglI GCCn_nnn'nGGC
Cuts at: 1996 1996
Size: 5570
BglII A'GATC_T
Cuts at: 811 1381 3085 5122 811
Size: 570 1704 2037 1259
Fragments arranged by size:
2037 1704 1259 570
BlpI GC'TnA_GC
Cuts at: 387 2682 387
Size: 2295 3275
Bme1580I G_kGCm'C
Cuts at: 1917 2001 2169 2337 3195 3288 4412 1917
Size: 84 168 168 858 93 1124 3075
Fragments arranged by size:
3075 1124 858 168 168 93 84
BmrI ACTGGGnnnn_n'
Cuts at: 1943 2782 2863 3198 5342 1943
Size: 839 81 335 2144 2171
Fragments arranged by size:
2171 2144 839 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3937 3937
Size: 5570
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 440 440
Size: 5570
Bpu10I CC'TnA_GC
Cuts at: 2100 2100
Size: 5570
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1306 2746 3625 4339 4637 1306
Size: 1440 879 714 298 2239
Fragments arranged by size:
2239 1440 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5274 5274
Size: 5570
BsaAI yAC'GTr
Cuts at: 2923 2923
Size: 5570
BsaBI GATnn'nnATC
Cuts at: 6 3084 5121 6
Size: 3078 2037 455
Fragments arranged by size:
3078 2037 455
BsaHI Gr'CG_yC
Cuts at: 3001 3249 3951 5447 3001
Size: 248 702 1496 3124
Fragments arranged by size:
3124 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2759 3280 3958 4369 4516 4790 2759
Size: 521 678 411 147 274 3539
Fragments arranged by size:
3539 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2995 3753 4418 4826 2995
Size: 758 665 408 3739
Fragments arranged by size:
3739 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 3112 3158 4388 104
Size: 3008 46 1230 1286
Fragments arranged by size:
3008 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 388 993 1161 1245 1413 1581 1917 2085 2169
Size: 605 168 84 168 168 336 168 84
Cuts at: 2169 2337 2584 3362 3552 4412 388
Size: 168 247 778 190 860 1546
Fragments arranged by size:
1546 860 778 605 336 247 190 168
168 168 168 168 84 84
BsmI GAATG_Cn'
Cuts at: 1201 2125 2386 2522 1201
Size: 924 261 136 4249
Fragments arranged by size:
4249 924 261 136
Bsp1286I G_dGCh'C
Cuts at: 388 993 1161 1245 1413 1581 1917 2001 2085
Size: 605 168 84 168 168 336 84 84
Cuts at: 2085 2169 2337 2584 3195 3288 3362 3552 3614
Size: 84 168 247 611 93 74 190 62
Cuts at: 3614 4412 388
Size: 798 1546
Fragments arranged by size:
1546 798 611 605 336 247 190 168
168 168 168 93 84 84 84 84
74 62
BspHI T'CATG_A
Cuts at: 4002 4002
Size: 5570
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2620 2834 3136 3517 2620
Size: 214 302 381 4673
Fragments arranged by size:
4673 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3862 3916 5400 5565 50
Size: 3812 54 1484 165 55
Fragments arranged by size:
3812 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3482 3482
Size: 5570
BsrFI r'CCGG_y
Cuts at: 3568 3749 3568
Size: 181 5389
BsrGI T'GTAC_A
Cuts at: 448 1074 448
Size: 626 4944
BssSI C'ACGA_G
Cuts at: 3841 4549 3841
Size: 708 4862
BstAPI GCAn_nnn'nTGC
Cuts at: 2947 2947
Size: 5570
BstBI TT'CG_AA
Cuts at: 3931 3931
Size: 5570
BstYI r'GATC_y
Cuts at: 1 811 1381 2595 3062 3085 3420 3666 3972
Size: 810 570 1214 467 23 335 246 306
Cuts at: 3972 3984 4070 5122 1
Size: 12 86 1052 449
Fragments arranged by size:
1214 1052 810 570 467 449 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1993 1993
Size: 5570
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3520 3701 3864 3520
Size: 181 163 5226
Fragments arranged by size:
5226 181 163
BtsI GCAGTG_nn'
Cuts at: 882 1206 2130 2813 882
Size: 324 924 683 3639
Fragments arranged by size:
3639 924 683 324
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 210 245 786 821 210
Size: 35 541 35 4959
Fragments arranged by size:
4959 541 35 35
DraI TTT'AAA
Cuts at: 2569 2569
Size: 5570
DrdI GACnn_nn'nnGTC
Cuts at: 3276 4620 3276
Size: 1344 4226
EaeI y'GGCC_r
Cuts at: 3155 3329 3720 3747 5092 3155
Size: 174 391 27 1345 3633
Fragments arranged by size:
3633 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3155 3155
Size: 5570
EarI CTCTTCn'nnn_
Cuts at: 3593 3803 3593
Size: 210 5360
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3778 4520 4666 5380 3778
Size: 742 146 714 3968
Fragments arranged by size:
3968 742 714 146
EcoICRI GAG'CTC
Cuts at: 386 2582 386
Size: 2196 3374
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 440 3395 3827 4180 4842 440
Size: 2955 432 353 662 1168
Fragments arranged by size:
2955 1168 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 599 2709 599
Size: 2110 3460
EcoRI G'AATT_C
Cuts at: 494 741 767 917 1001 1169 1253 1337 1421
Size: 247 26 150 84 168 84 84 84
Cuts at: 1421 1505 1673 1757 1841 1925 2009 2261 2345
Size: 84 168 84 84 84 84 252 84
Cuts at: 2345 2429 2574 494
Size: 84 145 3490
Fragments arranged by size:
3490 252 247 168 168 150 145 84
84 84 84 84 84 84 84 84
84 84 26
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3589 3589
Size: 5570
FspI TGC'GCA
Cuts at: 3351 3351
Size: 5570
HaeII r_GCGC'y
Cuts at: 3004 3252 4482 5253 3004
Size: 248 1230 771 3321
Fragments arranged by size:
3321 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 882 914 966 998 1146 1178 1398 1430 2058
Size: 32 52 32 148 32 220 32 628
Cuts at: 2058 2090 3412 3442 3444 3474 882
Size: 32 1322 30 2 30 2978
Fragments arranged by size:
2978 1322 628 220 148 52 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2609 2609
Size: 5570
HindIII A'AGCT_T
Cuts at: 728 2390 728
Size: 1662 3908
KasI G'GCGC_C
Cuts at: 3000 3248 3000
Size: 248 5322
KpnI G_GTAC'C
Cuts at: 2590 2590
Size: 5570
MluI A'CGCG_T
Cuts at: 5003 5003
Size: 5570
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4329 4513 4329
Size: 184 5386
MscI TGG'CCA
Cuts at: 3331 3331
Size: 5570
MslI CAynn'nnrTG
Cuts at: 253 1010 1070 1178 1910 2330 2515 3559 3686
Size: 757 60 108 732 420 185 1044 127
Cuts at: 3686 253
Size: 2137
Fragments arranged by size:
2137 1044 757 732 420 185 127 108
60
MspA1I CmG'CkG
Cuts at: 226 2681 2995 3355 4139 4384 226
Size: 2455 314 360 784 245 1412
Fragments arranged by size:
2455 1412 784 360 314 245
NaeI GCC'GGC
Cuts at: 3751 3751
Size: 5570
NarI GG'CG_CC
Cuts at: 3001 3249 3001
Size: 248 5322
NdeI CA'TA_TG
Cuts at: 2157 2157
Size: 5570
NgoMIV G'CCGG_C
Cuts at: 3749 3749
Size: 5570
NsiI A_TGCA'T
Cuts at: 2522 2522
Size: 5570
NspI r_CATG'y
Cuts at: 2546 2623 3123 4726 2546
Size: 77 500 1603 3390
Fragments arranged by size:
3390 1603 500 77
PciI A'CATG_T
Cuts at: 4722 4722
Size: 5570
PfoI T'CCnGG_A
Cuts at: 678 678
Size: 5570
PmeI GTTT'AAAC
Cuts at: 2569 2569
Size: 5570
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4016 4048 4016
Size: 32 5538
PstI C_TGCA'G
Cuts at: 2617 3302 2617
Size: 685 4885
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5570
PvuII CAG'CTG
Cuts at: 2995 3355 2995
Size: 360 5210
RsrII CG'GwC_CG
Cuts at: 3765 3765
Size: 5570
SacI G_AGCT'C
Cuts at: 388 2584 388
Size: 2196 3374
SalI G'TCGA_C
Cuts at: 2607 2607
Size: 5570
SapI GCTCTTCn'nnn_
Cuts at: 3593 3803 3593
Size: 210 5360
SbfI CC_TGCA'GG
Cuts at: 2617 2617
Size: 5570
ScaI AGT'ACT
Cuts at: 5087 5087
Size: 5570
SfcI C'TryA_G
Cuts at: 33 2613 3298 4266 4457 33
Size: 2580 685 968 191 1146
Fragments arranged by size:
2580 1146 968 685 191
SfoI GGC'GCC
Cuts at: 3002 3250 3002
Size: 248 5322
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5570
SmaI CCC'GGG
Cuts at: 2592 2592
Size: 5570
SmlI C'TyrA_G
Cuts at: 1321 2725 3640 4354 4616 1321
Size: 1404 915 714 262 2275
Fragments arranged by size:
2275 1404 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5570
SphI G_CATG'C
Cuts at: 2623 3123 2623
Size: 500 5070
StyI C'CwwG_G
Cuts at: 2704 3058 2704
Size: 354 5216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3153 3286 3745 3954 3153
Size: 133 459 209 4769
Fragments arranged by size:
4769 459 209 133
TatI w'GTAC_w
Cuts at: 399 448 1074 1660 2250 2418 5085 399
Size: 49 626 586 590 168 2667 884
Fragments arranged by size:
2667 884 626 590 586 168 49
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 195 227 674 706 5087 5119 195
Size: 32 447 32 4381 32 646
Fragments arranged by size:
4381 646 447 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3367 3367
Size: 5570
XbaI T'CTAG_A
Cuts at: 2601 2601
Size: 5570
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3065 3065
Size: 5570
XmaI C'CCGG_G
Cuts at: 2590 2590
Size: 5570
XmnI GAAnn'nnTTC
Cuts at: 730 964 1048 1720 1888 2056 2140 2308 730
Size: 234 84 672 168 168 84 168 3992
Fragments arranged by size:
3992 672 234 168 168 168 84 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI Bsu36I BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NsiI NspI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BmgBI BmtI
BsaXI BseRI BsgI BsiWI BsmBI BspEI BssHII BstEII
BstXI BstZ17I BtgI ClaI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NcoI NheI NotI NruI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI