Restriction map for
pF1KB9104
(Circular) (Six-base) MAPSORT of: pF1KB9104.seq Check: 586 from: 1 to: 4736
pF1KB9104 4736 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AatII G_ACGT'C
Cuts at: 1307 1307
Size: 4736
AccI GT'mk_AC
Cuts at: 1774 1774
Size: 4736
Acc65I G'GTAC_C
Cuts at: 1752 1752
Size: 4736
AclI AA'CG_TT
Cuts at: 535 4576 535
Size: 4041 695
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1192 2561 2993 3346 4008 1192
Size: 1369 432 353 662 1920
Fragments arranged by size:
1920 1369 662 432 353
AflIII A'CryG_T
Cuts at: 1360 2088 3888 4169 1360
Size: 728 1800 281 1927
Fragments arranged by size:
1927 1800 728 281
AleI CACnn'nnGTG
Cuts at: 1216 1216
Size: 4736
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1234 1234
Size: 4736
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 439 471 439
Size: 32 4704
AlwNI CAG_nnn'CTG
Cuts at: 936 2041 3479 936
Size: 1105 1438 2193
Fragments arranged by size:
2193 1438 1105
ApaLI G'TGCA_C
Cuts at: 3574 3574
Size: 4736
ApoI r'AATT_y
Cuts at: 1740 4089 4345 1740
Size: 2349 256 2131
Fragments arranged by size:
2349 2131 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4736
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4736
AvaI C'yCGr_G
Cuts at: 791 1756 791
Size: 965 3771
BamHI G'GATC_C
Cuts at: 133 1194 1761 133
Size: 1061 567 3108
Fragments arranged by size:
3108 1061 567
BanI G'GyrC_C
Cuts at: 1210 1218 1710 1752 2166 2414 2449 1210
Size: 8 492 42 414 248 35 3497
Fragments arranged by size:
3497 492 414 248 42 35 8
BanII G_rGCy'C
Cuts at: 368 683 740 920 1149 1670 1703 1750 2780
Size: 315 57 180 229 521 33 47 1030
Cuts at: 2780 368
Size: 2324
Fragments arranged by size:
2324 1030 521 315 229 180 57 47
33
BbeI G_GCGC'C
Cuts at: 2170 2418 2170
Size: 248 4488
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4702
BciVI GTATCCnnnnn_n'
Cuts at: 2634 3690 4736 2634
Size: 1056 1046 2634
Fragments arranged by size:
2634 1056 1046
BclI T'GATC_A
Cuts at: 2256 2256
Size: 4736
BglI GCCn_nnn'nGGC
Cuts at: 154 346 154
Size: 192 4544
BglII A'GATC_T
Cuts at: 2251 4288 2251
Size: 2037 2699
BlpI GC'TnA_GC
Cuts at: 320 596 1848 320
Size: 276 1252 3208
Fragments arranged by size:
3208 1252 276
Bme1580I G_kGCm'C
Cuts at: 1223 1616 2361 2454 3578 1223
Size: 393 745 93 1124 2381
Fragments arranged by size:
2381 1124 745 393 93
BmrI ACTGGGnnnn_n'
Cuts at: 137 1698 1948 2029 2364 4508 137
Size: 1561 250 81 335 2144 365
Fragments arranged by size:
2144 1561 365 335 250 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 817 3103 817
Size: 2286 2450
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 274 298 343 406 835 958 994 1345
Size: 15 24 45 63 429 123 36 351
Cuts at: 1345 1546 1654 1669 259
Size: 201 108 15 3326
Fragments arranged by size:
3326 429 351 201 123 108 63 45
36 24 15 15
Bpu10I CC'TnA_GC
Cuts at: 1148 1148
Size: 4736
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 1912 2791 3505 3803 697
Size: 1215 879 714 298 1630
Fragments arranged by size:
1630 1215 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1489 4440 1489
Size: 2951 1785
BsaAI yAC'GTr
Cuts at: 1363 1467 2089 1363
Size: 104 622 4010
Fragments arranged by size:
4010 622 104
BsaBI GATnn'nnATC
Cuts at: 6 1199 2250 4287 6
Size: 1193 1051 2037 455
Fragments arranged by size:
2037 1193 1051 455
BsaHI Gr'CG_yC
Cuts at: 1304 2167 2415 3117 4613 1304
Size: 863 248 702 1496 1427
Fragments arranged by size:
1496 1427 863 702 248
BsaWI w'CCGG_w
Cuts at: 1925 2446 3124 3535 3682 3956 1925
Size: 521 678 411 147 274 2705
Fragments arranged by size:
2705 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 256 955 1108 1597 256
Size: 699 153 489 3395
Fragments arranged by size:
3395 699 489 153
BseYI C'CCAG_C
Cuts at: 433 724 913 925 1312 1393 1721 2161 2919
Size: 291 189 12 387 81 328 440 758
Cuts at: 2919 3584 3992 433
Size: 665 408 1177
Fragments arranged by size:
1177 758 665 440 408 387 328 291
189 81 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 307
Size: 4736
BsiEI CG_ry'CG
Cuts at: 104 1240 1451 2278 2324 3554 104
Size: 1136 211 827 46 1230 1286
Fragments arranged by size:
1286 1230 1136 827 211 46
BsiHKAI G_wGCw'C
Cuts at: 683 1670 1750 2528 2718 3578 683
Size: 987 80 778 190 860 1841
Fragments arranged by size:
1841 987 860 778 190 80
BsmI GAATG_Cn'
Cuts at: 572 1062 572
Size: 490 4246
BsmBI CGTCTCn'nnnn_
Cuts at: 238 382 1563 238
Size: 144 1181 3411
Fragments arranged by size:
3411 1181 144
Bsp1286I G_dGCh'C
Cuts at: 368 683 740 920 1149 1223 1616 1670 1703
Size: 315 57 180 229 74 393 54 33
Cuts at: 1703 1750 2361 2454 2528 2718 2780 3578 368
Size: 47 611 93 74 190 62 798 1526
Fragments arranged by size:
1526 798 611 393 315 229 190 180
93 74 74 62 57 54 47 33
BspHI T'CATG_A
Cuts at: 1074 3168 1074
Size: 2094 2642
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1786 2000 2302 2683 1786
Size: 214 302 381 3839
Fragments arranged by size:
3839 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1254 3028 3082 4566 4731 50
Size: 1204 1774 54 1484 165 55
Fragments arranged by size:
1774 1484 1204 165 55 54
BsrDI GCAATG_nn'
Cuts at: 696 2648 696
Size: 1952 2784
BsrFI r'CCGG_y
Cuts at: 2734 2915 2734
Size: 181 4555
BssHII G'CGCG_C
Cuts at: 568 568
Size: 4736
BssSI C'ACGA_G
Cuts at: 3007 3715 3007
Size: 708 4028
BstAPI GCAn_nnn'nTGC
Cuts at: 606 2113 606
Size: 1507 3229
BstBI TT'CG_AA
Cuts at: 3097 3097
Size: 4736
BstYI r'GATC_y
Cuts at: 1 133 211 293 716 1194 1761 2228 2251
Size: 132 78 82 423 478 567 467 23
Cuts at: 2251 2586 2832 3138 3150 3236 4288 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 567 478 467 449 423 335 306
246 132 86 82 78 23 12
BtgI C'CryG_G
Cuts at: 149 1214 1284 1679 149
Size: 1065 70 395 3206
Fragments arranged by size:
3206 1065 395 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2686 2867 3030 2686
Size: 181 163 4392
Fragments arranged by size:
4392 181 163
BtsI GCAGTG_nn'
Cuts at: 663 670 708 915 1979 663
Size: 7 38 207 1064 3420
Fragments arranged by size:
3420 1064 207 38 7
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 678 713 678
Size: 35 4701
DraI TTT'AAA
Cuts at: 1735 1735
Size: 4736
DrdI GACnn_nn'nnGTC
Cuts at: 2442 3786 2442
Size: 1344 3392
EaeI y'GGCC_r
Cuts at: 218 1237 1448 2321 2495 2886 2913 4258 218
Size: 1019 211 873 174 391 27 1345 696
Fragments arranged by size:
1345 1019 873 696 391 211 174 27
EagI C'GGCC_G
Cuts at: 1237 1448 2321 1237
Size: 211 873 3652
Fragments arranged by size:
3652 873 211
EarI CTCTTCn'nnn_
Cuts at: 2759 2969 2759
Size: 210 4526
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1108 1576 2944 3686 3832 4546 1108
Size: 468 1368 742 146 714 1298
Fragments arranged by size:
1368 1298 742 714 468 146
EcoICRI GAG'CTC
Cuts at: 681 1668 1748 681
Size: 987 80 3669
Fragments arranged by size:
3669 987 80
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 274 298 343 406 835 958 994 1192
Size: 15 24 45 63 429 123 36 198
Cuts at: 1192 1345 1546 1654 1669 2561 2993 3346 4008
Size: 153 201 108 15 892 432 353 662
Cuts at: 4008 259
Size: 987
Fragments arranged by size:
987 892 662 432 429 353 201 198
153 123 108 63 45 36 24 15
15
EcoNI CCTnn'n_nnAGG
Cuts at: 220 220
Size: 4736
EcoO109I rG'GnC_Cy
Cuts at: 303 969 995 1482 1655 1875 303
Size: 666 26 487 173 220 3164
Fragments arranged by size:
3164 666 487 220 173 26
EcoRI G'AATT_C
Cuts at: 1740 1740
Size: 4736
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2755 2755
Size: 4736
FspI TGC'GCA
Cuts at: 490 688 2517 490
Size: 198 1829 2709
Fragments arranged by size:
2709 1829 198
FspAI rTGC'GCAy
Cuts at: 490 490
Size: 4736
HaeII r_GCGC'y
Cuts at: 1320 2170 2418 3648 4419 1320
Size: 850 248 1230 771 1637
Fragments arranged by size:
1637 1230 850 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1131 1163 2578 2608 2610 2640 1131
Size: 32 1415 30 2 30 3227
Fragments arranged by size:
3227 1415 32 30 30 2
HincII GTy'rAC
Cuts at: 1419 1775 1419
Size: 356 4380
KasI G'GCGC_C
Cuts at: 2166 2414 2166
Size: 248 4488
KpnI G_GTAC'C
Cuts at: 1756 1756
Size: 4736
MluI A'CGCG_T
Cuts at: 4169 4169
Size: 4736
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3495 3679 3495
Size: 184 4552
MscI TGG'CCA
Cuts at: 220 2497 220
Size: 2277 2459
MslI CAynn'nnrTG
Cuts at: 527 617 1216 2725 2852 527
Size: 90 599 1509 127 2411
Fragments arranged by size:
2411 1509 599 127 90
MspA1I CmG'CkG
Cuts at: 315 933 1602 1721 1847 2161 2521 3305 3550
Size: 618 669 119 126 314 360 784 245
Cuts at: 3550 315
Size: 1501
Fragments arranged by size:
1501 784 669 618 360 314 245 126
119
NaeI GCC'GGC
Cuts at: 2917 2917
Size: 4736
NarI GG'CG_CC
Cuts at: 2167 2415 2167
Size: 248 4488
NcoI C'CATG_G
Cuts at: 1679 1679
Size: 4736
NgoMIV G'CCGG_C
Cuts at: 2915 2915
Size: 4736
NspI r_CATG'y
Cuts at: 616 893 1110 1789 2289 3892 616
Size: 277 217 679 500 1603 1460
Fragments arranged by size:
1603 1460 679 500 277 217
PciI A'CATG_T
Cuts at: 3888 3888
Size: 4736
PflMI CCAn_nnn'nTGG
Cuts at: 881 936 881
Size: 55 4681
PfoI T'CCnGG_A
Cuts at: 119 359 383 119
Size: 240 24 4472
Fragments arranged by size:
4472 240 24
PmeI GTTT'AAAC
Cuts at: 1735 1735
Size: 4736
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3182 3214 3182
Size: 32 4704
PpuMI rG'GwC_Cy
Cuts at: 303 969 995 1655 303
Size: 666 26 660 3384
Fragments arranged by size:
3384 666 660 26
PstI C_TGCA'G
Cuts at: 162 677 1783 2468 162
Size: 515 1106 685 2430
Fragments arranged by size:
2430 1106 685 515
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4736
PvuII CAG'CTG
Cuts at: 315 933 1721 2161 2521 315
Size: 618 788 440 360 2530
Fragments arranged by size:
2530 788 618 440 360
RsrII CG'GwC_CG
Cuts at: 2931 2931
Size: 4736
SacI G_AGCT'C
Cuts at: 683 1670 1750 683
Size: 987 80 3669
Fragments arranged by size:
3669 987 80
SalI G'TCGA_C
Cuts at: 1773 1773
Size: 4736
SanDI GG'GwC_CC
Cuts at: 995 995
Size: 4736
SapI GCTCTTCn'nnn_
Cuts at: 2759 2969 2759
Size: 210 4526
SbfI CC_TGCA'GG
Cuts at: 1783 1783
Size: 4736
ScaI AGT'ACT
Cuts at: 4253 4253
Size: 4736
SfcI C'TryA_G
Cuts at: 33 158 673 1779 2464 3432 3623 33
Size: 125 515 1106 685 968 191 1146
Fragments arranged by size:
1146 1106 968 685 515 191 125
SfoI GGC'GCC
Cuts at: 2168 2416 2168
Size: 248 4488
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4736
SmaI CCC'GGG
Cuts at: 1758 1758
Size: 4736
SmlI C'TyrA_G
Cuts at: 712 1891 2806 3520 3782 712
Size: 1179 915 714 262 1666
Fragments arranged by size:
1666 1179 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4736
SphI G_CATG'C
Cuts at: 893 1110 1789 2289 893
Size: 217 679 500 3340
Fragments arranged by size:
3340 679 500 217
StuI AGG'CCT
Cuts at: 605 824 605
Size: 219 4517
StyI C'CwwG_G
Cuts at: 221 1679 1870 2224 221
Size: 1458 191 354 2733
Fragments arranged by size:
2733 1458 354 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 517 607 1698 1713 2319 2452 2911 3120 517
Size: 90 1091 15 606 133 459 209 2133
Fragments arranged by size:
2133 1091 606 459 209 133 90 15
TatI w'GTAC_w
Cuts at: 4251 4251
Size: 4736
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 361 393 4253 4285 361
Size: 32 3860 32 812
Fragments arranged by size:
3860 812 32 32
Tth111I GACn'n_nGTC
Cuts at: 2533 2533
Size: 4736
XbaI T'CTAG_A
Cuts at: 1767 1767
Size: 4736
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 441 933 1380 2231 441
Size: 492 447 851 2946
Fragments arranged by size:
2946 851 492 447
XmaI C'CCGG_G
Cuts at: 1756 1756
Size: 4736
ZraI GAC'GTC
Cuts at: 1305 1305
Size: 4736
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI ApaI AscI AvrII
BaeI BbsI BbvCI BfrBI BmgBI BmtI BsaXI BsiWI
BspEI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI DraIII
EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI