Restriction map for
pF1KB9101
(Circular) (Six-base) MAPSORT of: pF1KB9101.seq Check: 6875 from: 1 to: 6587
pF1KB9101 6587 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2493 2493
Size: 6587
AccI GT'mk_AC
Cuts at: 3625 3625
Size: 6587
Acc65I G'GTAC_C
Cuts at: 3603 3603
Size: 6587
AclI AA'CG_TT
Cuts at: 1978 6427 1978
Size: 4449 2138
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 4412 4844 5197 5859 502
Size: 3910 432 353 662 1230
Fragments arranged by size:
3910 1230 662 432 353
AflII C'TTAA_G
Cuts at: 2304 2304
Size: 6587
AflIII A'CryG_T
Cuts at: 3939 5739 6020 3939
Size: 1800 281 4506
Fragments arranged by size:
4506 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 225 2239 225
Size: 2014 4573
AleI CACnn'nnGTG
Cuts at: 2413 2413
Size: 6587
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 563 608 563
Size: 45 6542
AlwNI CAG_nnn'CTG
Cuts at: 211 1468 2188 3187 3892 5330 211
Size: 1257 720 999 705 1438 1468
Fragments arranged by size:
1468 1438 1257 999 720 705
ApaLI G'TGCA_C
Cuts at: 139 1961 2416 5425 139
Size: 1822 455 3009 1301
Fragments arranged by size:
3009 1822 1301 455
ApoI r'AATT_y
Cuts at: 426 1572 1839 2004 2399 2809 2830 3031 3591
Size: 1146 267 165 395 410 21 201 560
Cuts at: 3591 5940 6196 426
Size: 2349 256 817
Fragments arranged by size:
2349 1146 817 560 410 395 267 256
201 165 21
AseI AT'TA_AT
Cuts at: 20 20
Size: 6587
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6587
AvaI C'yCGr_G
Cuts at: 947 3607 947
Size: 2660 3927
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 200 233 200
Size: 33 6554
BamHI G'GATC_C
Cuts at: 1521 3612 1521
Size: 2091 4496
BanI G'GyrC_C
Cuts at: 3603 4017 4265 4300 3603
Size: 414 248 35 5890
Fragments arranged by size:
5890 414 248 35
BanII G_rGCy'C
Cuts at: 3601 4631 3601
Size: 1030 5557
BbeI G_GCGC'C
Cuts at: 4021 4269 4021
Size: 248 6339
BbsI GAAGACnn'nnnn_
Cuts at: 365 2510 365
Size: 2145 4442
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 928 962 928
Size: 34 6553
BciVI GTATCCnnnnn_n'
Cuts at: 4485 5541 6587 4485
Size: 1056 1046 4485
Fragments arranged by size:
4485 1056 1046
BclI T'GATC_A
Cuts at: 4107 4107
Size: 6587
BfrBI ATG'CAT
Cuts at: 186 186
Size: 6587
BglII A'GATC_T
Cuts at: 4102 6139 4102
Size: 2037 4550
BlpI GC'TnA_GC
Cuts at: 2856 3699 2856
Size: 843 5744
Bme1580I G_kGCm'C
Cuts at: 143 1965 2420 2445 2766 4212 4305 5429 143
Size: 1822 455 25 321 1446 93 1124 1301
Fragments arranged by size:
1822 1446 1301 1124 455 321 93 25
BmrI ACTGGGnnnn_n'
Cuts at: 1730 3209 3799 3880 4215 6359 1730
Size: 1479 590 81 335 2144 1958
Fragments arranged by size:
2144 1958 1479 590 335 81
BmtI G_CTAG'C
Cuts at: 457 1124 457
Size: 667 5920
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 147 4954 147
Size: 4807 1780
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1739 2230 1739
Size: 491 6096
Bpu10I CC'TnA_GC
Cuts at: 1277 3152 1277
Size: 1875 4712
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 2442 3763 4642 5356 5654 562
Size: 1880 1321 879 714 298 1495
Fragments arranged by size:
1880 1495 1321 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1802 2046 6291 1802
Size: 244 4245 2098
Fragments arranged by size:
4245 2098 244
BsaAI yAC'GTr
Cuts at: 3940 3940
Size: 6587
BsaBI GATnn'nnATC
Cuts at: 6 710 1367 4101 6138 6
Size: 704 657 2734 2037 455
Fragments arranged by size:
2734 2037 704 657 455
BsaHI Gr'CG_yC
Cuts at: 4018 4266 4968 6464 4018
Size: 248 702 1496 4141
Fragments arranged by size:
4141 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3776 4297 4975 5386 5533 5807 3776
Size: 521 678 411 147 274 4556
Fragments arranged by size:
4556 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 752 752
Size: 6587
BseYI C'CCAG_C
Cuts at: 232 2056 3079 4012 4770 5435 5843 232
Size: 1824 1023 933 758 665 408 976
Fragments arranged by size:
1824 1023 976 933 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 2491 3188 328
Size: 2163 697 3727
Fragments arranged by size:
3727 2163 697
BsiEI CG_ry'CG
Cuts at: 104 4129 4175 5405 104
Size: 4025 46 1230 1286
Fragments arranged by size:
4025 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 143 1765 1965 2420 3158 3601 4379 4569 5429
Size: 1622 200 455 738 443 778 190 860
Cuts at: 5429 143
Size: 1301
Fragments arranged by size:
1622 1301 860 778 738 455 443 200
190
BsmI GAATG_Cn'
Cuts at: 393 2027 393
Size: 1634 4953
Bsp1286I G_dGCh'C
Cuts at: 143 1765 1965 2420 2445 2766 3158 3601 4212
Size: 1622 200 455 25 321 392 443 611
Cuts at: 4212 4305 4379 4569 4631 5429 143
Size: 93 74 190 62 798 1301
Fragments arranged by size:
1622 1301 798 611 455 443 392 321
200 190 93 74 62 25
BspHI T'CATG_A
Cuts at: 422 603 794 5019 422
Size: 181 191 4225 1990
Fragments arranged by size:
4225 1990 191 181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1177 2493 3637 3851 4153 4534 1177
Size: 1316 1144 214 302 381 3230
Fragments arranged by size:
3230 1316 1144 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4879 4933 6417 6582 50
Size: 4829 54 1484 165 55
Fragments arranged by size:
4829 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4499 4499
Size: 6587
BsrFI r'CCGG_y
Cuts at: 4585 4766 4585
Size: 181 6406
BsrGI T'GTAC_A
Cuts at: 3062 3062
Size: 6587
BssSI C'ACGA_G
Cuts at: 4858 5566 4858
Size: 708 5879
BstAPI GCAn_nnn'nTGC
Cuts at: 3964 3964
Size: 6587
BstBI TT'CG_AA
Cuts at: 4948 4948
Size: 6587
BstXI CCAn_nnnn'nTGG
Cuts at: 1084 2040 1084
Size: 956 5631
BstYI r'GATC_y
Cuts at: 1 331 1521 3612 4079 4102 4437 4683 4989
Size: 330 1190 2091 467 23 335 246 306
Cuts at: 4989 5001 5087 6139 1
Size: 12 86 1052 449
Fragments arranged by size:
2091 1190 1052 467 449 335 330 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1664 2531 1664
Size: 867 5720
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4537 4718 4881 4537
Size: 181 163 6243
Fragments arranged by size:
6243 181 163
BtsI GCAGTG_nn'
Cuts at: 279 1398 3830 279
Size: 1119 2432 3036
Fragments arranged by size:
3036 2432 1119
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 827 862 827
Size: 35 6552
DraI TTT'AAA
Cuts at: 431 614 962 2511 2718 2835 3050 3586 431
Size: 183 348 1549 207 117 215 536 3432
Fragments arranged by size:
3432 1549 536 348 215 207 183 117
DraIII CAC_nnn'GTG
Cuts at: 2017 2017
Size: 6587
DrdI GACnn_nn'nnGTC
Cuts at: 4293 5637 4293
Size: 1344 5243
EaeI y'GGCC_r
Cuts at: 1010 1029 1623 4172 4346 4737 4764 6109 1010
Size: 19 594 2549 174 391 27 1345 1488
Fragments arranged by size:
2549 1488 1345 594 391 174 27 19
EagI C'GGCC_G
Cuts at: 4172 4172
Size: 6587
EarI CTCTTCn'nnn_
Cuts at: 116 239 397 2039 2272 2795 3419 4610 4820
Size: 123 158 1642 233 523 624 1191 210
Cuts at: 4820 116
Size: 1883
Fragments arranged by size:
1883 1642 1191 624 523 233 210 158
123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4795 5537 5683 6397 4795
Size: 742 146 714 4985
Fragments arranged by size:
4985 742 714 146
EcoICRI GAG'CTC
Cuts at: 3599 3599
Size: 6587
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 1739 2230 4412 4844 5197 5859 502
Size: 1237 491 2182 432 353 662 1230
Fragments arranged by size:
2182 1237 1230 662 491 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 2192 2192
Size: 6587
EcoO109I rG'GnC_Cy
Cuts at: 2185 2783 3226 3726 2185
Size: 598 443 500 5046
Fragments arranged by size:
5046 598 500 443
EcoRI G'AATT_C
Cuts at: 1572 1839 3591 1572
Size: 267 1752 4568
Fragments arranged by size:
4568 1752 267
EcoRV GAT'ATC
Cuts at: 1545 1545
Size: 6587
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1621 3448 3494 4606 1621
Size: 1827 46 1112 3602
Fragments arranged by size:
3602 1827 1112 46
FspI TGC'GCA
Cuts at: 4368 4368
Size: 6587
HaeII r_GCGC'y
Cuts at: 4021 4269 5499 6270 4021
Size: 248 1230 771 4338
Fragments arranged by size:
4338 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 211 243 534 566 581 613 2225 2257 2497
Size: 32 291 32 15 32 1612 32 240
Cuts at: 2497 2529 3373 3405 4429 4459 4461 4491 211
Size: 32 844 32 1024 30 2 30 2307
Fragments arranged by size:
2307 1612 1024 844 291 240 32 32
32 32 32 32 30 30 15 2
HincII GTy'rAC
Cuts at: 219 468 2072 2176 3626 219
Size: 249 1604 104 1450 3180
Fragments arranged by size:
3180 1604 1450 249 104
HindIII A'AGCT_T
Cuts at: 446 2686 2901 3452 446
Size: 2240 215 551 3581
Fragments arranged by size:
3581 2240 551 215
HpaI GTT'AAC
Cuts at: 2072 2072
Size: 6587
KasI G'GCGC_C
Cuts at: 4017 4265 4017
Size: 248 6339
KpnI G_GTAC'C
Cuts at: 3607 3607
Size: 6587
MfeI C'AATT_G
Cuts at: 1612 1612
Size: 6587
MluI A'CGCG_T
Cuts at: 6020 6020
Size: 6587
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 753 1439 5346 5530 753
Size: 686 3907 184 1810
Fragments arranged by size:
3907 1810 686 184
MscI TGG'CCA
Cuts at: 1012 1031 1625 4348 1012
Size: 19 594 2723 3251
Fragments arranged by size:
3251 2723 594 19
MslI CAynn'nnrTG
Cuts at: 1082 1631 1679 2038 2413 4576 4703 1082
Size: 549 48 359 375 2163 127 2966
Fragments arranged by size:
2966 2163 549 375 359 127 48
MspA1I CmG'CkG
Cuts at: 2067 2922 3698 4012 4372 5156 5401 2067
Size: 855 776 314 360 784 245 3253
Fragments arranged by size:
3253 855 784 776 360 314 245
NaeI GCC'GGC
Cuts at: 4768 4768
Size: 6587
NarI GG'CG_CC
Cuts at: 4018 4266 4018
Size: 248 6339
NdeI CA'TA_TG
Cuts at: 1851 3491 1851
Size: 1640 4947
NgoMIV G'CCGG_C
Cuts at: 4766 4766
Size: 6587
NheI G'CTAG_C
Cuts at: 453 1120 453
Size: 667 5920
NsiI A_TGCA'T
Cuts at: 188 188
Size: 6587
NspI r_CATG'y
Cuts at: 342 3321 3640 4140 5743 342
Size: 2979 319 500 1603 1186
Fragments arranged by size:
2979 1603 1186 500 319
PciI A'CATG_T
Cuts at: 5739 5739
Size: 6587
PflMI CCAn_nnn'nTGG
Cuts at: 1288 1905 3187 3236 1288
Size: 617 1282 49 4639
Fragments arranged by size:
4639 1282 617 49
PmeI GTTT'AAAC
Cuts at: 3586 3586
Size: 6587
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5033 5065 5033
Size: 32 6555
PpuMI rG'GwC_Cy
Cuts at: 2185 2783 3226 2185
Size: 598 443 5546
Fragments arranged by size:
5546 598 443
PshAI GACnn'nnGTC
Cuts at: 2618 2618
Size: 6587
PsiI TTA'TAA
Cuts at: 1193 1193
Size: 6587
PstI C_TGCA'G
Cuts at: 542 1861 3444 3634 4319 542
Size: 1319 1583 190 685 2810
Fragments arranged by size:
2810 1583 1319 685 190
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6587
PvuII CAG'CTG
Cuts at: 2067 2922 4012 4372 2067
Size: 855 1090 360 4282
Fragments arranged by size:
4282 1090 855 360
RsrII CG'GwC_CG
Cuts at: 4782 4782
Size: 6587
SacI G_AGCT'C
Cuts at: 3601 3601
Size: 6587
SalI G'TCGA_C
Cuts at: 3624 3624
Size: 6587
SapI GCTCTTCn'nnn_
Cuts at: 116 2039 2795 4610 4820 116
Size: 1923 756 1815 210 1883
Fragments arranged by size:
1923 1883 1815 756 210
SbfI CC_TGCA'GG
Cuts at: 3634 3634
Size: 6587
ScaI AGT'ACT
Cuts at: 1863 2319 2774 6104 1863
Size: 456 455 3330 2346
Fragments arranged by size:
3330 2346 456 455
SfcI C'TryA_G
Cuts at: 33 538 1136 1857 3440 3630 4315 5283 5474
Size: 505 598 721 1583 190 685 968 191
Cuts at: 5474 33
Size: 1146
Fragments arranged by size:
1583 1146 968 721 685 598 505 191
190
SfoI GGC'GCC
Cuts at: 4019 4267 4019
Size: 248 6339
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6587
SmaI CCC'GGG
Cuts at: 3609 3609
Size: 6587
SmlI C'TyrA_G
Cuts at: 577 947 2304 2421 3742 4657 5371 5633 577
Size: 370 1357 117 1321 915 714 262 1531
Fragments arranged by size:
1531 1357 1321 915 714 370 262 117
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6587
SphI G_CATG'C
Cuts at: 342 3640 4140 342
Size: 3298 500 2789
Fragments arranged by size:
3298 2789 500
SspI AAT'ATT
Cuts at: 1357 1357
Size: 6587
StyI C'CwwG_G
Cuts at: 3721 4075 3721
Size: 354 6233
SwaI ATTT'AAAT
Cuts at: 2835 2835
Size: 6587
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3191 4170 4303 4762 4971 3191
Size: 979 133 459 209 4807
Fragments arranged by size:
4807 979 459 209 133
TatI w'GTAC_w
Cuts at: 1333 1671 1738 1861 2317 2772 3062 6102 1333
Size: 338 67 123 456 455 290 3040 1818
Fragments arranged by size:
3040 1818 456 455 338 290 123 67
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 619 651 1506 1538 6104 6136 619
Size: 32 855 32 4566 32 1070
Fragments arranged by size:
4566 1070 855 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1295 4384 1295
Size: 3089 3498
XbaI T'CTAG_A
Cuts at: 2646 3175 3618 2646
Size: 529 443 5615
Fragments arranged by size:
5615 529 443
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1039 3087 4082 1039
Size: 2048 995 3544
Fragments arranged by size:
3544 2048 995
XhoI C'TCGA_G
Cuts at: 947 947
Size: 6587
XmaI C'CCGG_G
Cuts at: 3607 3607
Size: 6587
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NheI NsiI
NspI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AgeI AloI ApaI AscI AvrII BbvCI
BglI BmgBI BsaXI BsiWI BsmBI BspEI BssHII BstEII
BstZ17I BtgI ClaI FseI FspAI NcoI NotI NruI
PacI PasI PfoI PmlI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI XmnI
ZraI