Restriction map for
pF1KB9100
(Circular) (Six-base) MAPSORT of: pF1KB9100.seq Check: 710 from: 1 to: 6437
pF1KB9100 6437 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2087 2087
Size: 6437
AccI GT'mk_AC
Cuts at: 125 3112 3381 3475 125
Size: 2987 269 94 3087
Fragments arranged by size:
3087 2987 269 94
Acc65I G'GTAC_C
Cuts at: 3453 3453
Size: 6437
AclI AA'CG_TT
Cuts at: 6277 6277
Size: 6437
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 1546 2563 2764 4262 4694 5441 5709 244
Size: 1302 1017 201 1498 432 747 268 972
Fragments arranged by size:
1498 1302 1017 972 747 432 268 201
AflIII A'CryG_T
Cuts at: 1502 3789 4893 5870 1502
Size: 2287 1104 977 2069
Fragments arranged by size:
2287 2069 1104 977
AgeI A'CCGG_T
Cuts at: 1058 4825 1058
Size: 3767 2670
AhdI GACnn_n'nnGTC
Cuts at: 3129 3129
Size: 6437
AleI CACnn'nnGTG
Cuts at: 164 164
Size: 6437
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1738 1738
Size: 6437
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 589 621 589
Size: 32 6405
AlwNI CAG_nnn'CTG
Cuts at: 469 1548 1898 3402 3742 5309 469
Size: 1079 350 1504 340 1567 1597
Fragments arranged by size:
1597 1567 1504 1079 350 340
ApaI G_GGCC'C
Cuts at: 2515 2770 2515
Size: 255 6182
ApaLI G'TGCA_C
Cuts at: 5207 5207
Size: 6437
ApoI r'AATT_y
Cuts at: 1999 2230 2662 2722 3441 5790 6046 1999
Size: 231 432 60 719 2349 256 2390
Fragments arranged by size:
2390 2349 719 432 256 231 60
AseI AT'TA_AT
Cuts at: 20 274 20
Size: 254 6183
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6437
AvaI C'yCGr_G
Cuts at: 828 1160 1190 2149 2204 3457 828
Size: 332 30 959 55 1253 3808
Fragments arranged by size:
3808 1253 959 332 55 30
BamHI G'GATC_C
Cuts at: 3462 3462
Size: 6437
BanI G'GyrC_C
Cuts at: 760 1775 3264 3453 3867 4115 4150 760
Size: 1015 1489 189 414 248 35 3047
Fragments arranged by size:
3047 1489 1015 414 248 189 35
BanII G_rGCy'C
Cuts at: 835 2515 2770 2788 3451 4481 835
Size: 1680 255 18 663 1030 2791
Fragments arranged by size:
2791 1680 1030 663 255 18
BbeI G_GCGC'C
Cuts at: 764 1779 3871 4119 764
Size: 1015 2092 248 3082
Fragments arranged by size:
3082 2092 1015 248
BbsI GAAGACnn'nnnn_
Cuts at: 2643 2704 2643
Size: 61 6376
BciVI GTATCCnnnnn_n'
Cuts at: 1650 2980 4335 5096 6437 1650
Size: 1330 1355 761 1341 1650
Fragments arranged by size:
1650 1355 1341 1330 761
BclI T'GATC_A
Cuts at: 404 3957 404
Size: 3553 2884
BfrBI ATG'CAT
Cuts at: 1518 1518
Size: 6437
BglI GCCn_nnn'nGGC
Cuts at: 830 2046 830
Size: 1216 5221
BglII A'GATC_T
Cuts at: 671 3952 5989 671
Size: 3281 2037 1119
Fragments arranged by size:
3281 2037 1119
BlpI GC'TnA_GC
Cuts at: 3549 3549
Size: 6437
Bme1580I G_kGCm'C
Cuts at: 2515 2770 4062 4155 5211 2515
Size: 255 1292 93 1056 3741
Fragments arranged by size:
3741 1292 1056 255 93
BmgBI CAC'GTC
Cuts at: 1503 1503
Size: 6437
BmrI ACTGGGnnnn_n'
Cuts at: 1908 2772 2834 2953 3649 3730 4065 6209 1908
Size: 864 62 119 696 81 335 2144 2136
Fragments arranged by size:
2144 2136 864 696 335 119 81 62
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 780 2476 4804 780
Size: 1696 2328 2413
Fragments arranged by size:
2413 2328 1696
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 480 527 586 591 1537 2353 2793 480
Size: 47 59 5 946 816 440 4124
Fragments arranged by size:
4124 946 816 440 59 47 5
Bpu10I CC'TnA_GC
Cuts at: 820 2668 3299 820
Size: 1848 631 3958
Fragments arranged by size:
3958 1848 631
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2818 3145 3613 4492 4984 5282 2818
Size: 327 468 879 492 298 3973
Fragments arranged by size:
3973 879 492 468 327 298
BsaI GGTCTCn'nnnn_
Cuts at: 2447 6141 2447
Size: 3694 2743
BsaAI yAC'GTr
Cuts at: 531 1128 3790 531
Size: 597 2662 3178
Fragments arranged by size:
3178 2662 597
BsaBI GATnn'nnATC
Cuts at: 6 904 3951 5988 6
Size: 898 3047 2037 455
Fragments arranged by size:
3047 2037 898 455
BsaHI Gr'CG_yC
Cuts at: 761 1262 1776 2069 3139 3868 4116 4818 6314
Size: 501 514 293 1070 729 248 702 1496
Cuts at: 6314 761
Size: 884
Fragments arranged by size:
1496 1070 884 729 702 514 501 293
248
BsaWI w'CCGG_w
Cuts at: 593 1058 1746 3626 4147 4825 5099 5246 5657
Size: 465 688 1880 521 678 274 147 411
Cuts at: 5657 593
Size: 1373
Fragments arranged by size:
1880 1373 688 678 521 465 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 589 619 2298 2328 589
Size: 30 1679 30 4698
Fragments arranged by size:
4698 1679 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 524 2071 2139 2707 2966 524
Size: 1547 68 568 259 3995
Fragments arranged by size:
3995 1547 568 259 68
BseYI C'CCAG_C
Cuts at: 1630 2118 3219 3242 3862 4620 5197 5693 1630
Size: 488 1101 23 620 758 577 496 2374
Fragments arranged by size:
2374 1101 758 620 577 496 488 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 1168 2059 2798 2818 868
Size: 300 891 739 20 4487
Fragments arranged by size:
4487 891 739 300 20
BsiEI CG_ry'CG
Cuts at: 104 3979 4025 5233 104
Size: 3875 46 1208 1308
Fragments arranged by size:
3875 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1589 1831 2360 3451 4229 4419 5211 1589
Size: 242 529 1091 778 190 792 2815
Fragments arranged by size:
2815 1091 792 778 529 242 190
Bsp1286I G_dGCh'C
Cuts at: 835 1589 1831 2360 2515 2770 2788 3451 4062
Size: 754 242 529 155 255 18 663 611
Cuts at: 4062 4155 4229 4419 4481 5211 835
Size: 93 74 190 62 730 2061
Fragments arranged by size:
2061 754 730 663 611 529 255 242
190 155 93 74 62 18
BspEI T'CCGG_A
Cuts at: 593 1746 5657 593
Size: 1153 3911 1373
Fragments arranged by size:
3911 1373 1153
BspHI T'CATG_A
Cuts at: 482 1484 1581 5613 482
Size: 1002 97 4032 1306
Fragments arranged by size:
4032 1306 1002 97
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2087 2465 2847 3487 3701 4003 4384 2087
Size: 378 382 640 214 302 381 4140
Fragments arranged by size:
4140 640 382 381 378 302 214
BsrBI CCG'CTC
Cuts at: 50 343 726 4729 4783 6267 6432 50
Size: 293 383 4003 54 1484 165 55
Fragments arranged by size:
4003 1484 383 293 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4349 4349
Size: 6437
BsrFI r'CCGG_y
Cuts at: 1058 1082 4435 4616 4825 1058
Size: 24 3353 181 209 2670
Fragments arranged by size:
3353 2670 209 181 24
BsrGI T'GTAC_A
Cuts at: 392 3071 392
Size: 2679 3758
BssSI C'ACGA_G
Cuts at: 4708 5066 4708
Size: 358 6079
BstAPI GCAn_nnn'nTGC
Cuts at: 2082 3814 2082
Size: 1732 4705
BstBI TT'CG_AA
Cuts at: 4798 4798
Size: 6437
BstXI CCAn_nnnn'nTGG
Cuts at: 1300 2815 3040 1300
Size: 1515 225 4697
Fragments arranged by size:
4697 1515 225
BstYI r'GATC_y
Cuts at: 1 265 671 905 1692 2567 3357 3462 3929
Size: 264 406 234 787 875 790 105 467
Cuts at: 3929 3952 4287 4533 5545 5631 5643 5989 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 875 790 787 467 449 406 346
335 264 246 234 105 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3260 3260
Size: 6437
BtgI C'CryG_G
Cuts at: 107 1229 3080 107
Size: 1122 1851 3464
Fragments arranged by size:
3464 1851 1122
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4387 4568 4731 4387
Size: 181 163 6093
Fragments arranged by size:
6093 181 163
BtsI GCAGTG_nn'
Cuts at: 3680 3680
Size: 6437
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 144 179 144
Size: 35 6402
DraI TTT'AAA
Cuts at: 3436 3436
Size: 6437
DrdI GACnn_nn'nnGTC
Cuts at: 2827 4143 5001 2827
Size: 1316 858 4263
Fragments arranged by size:
4263 1316 858
EaeI y'GGCC_r
Cuts at: 4022 4196 4587 4614 5959 4022
Size: 174 391 27 1345 4500
Fragments arranged by size:
4500 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4022 4022
Size: 6437
EarI CTCTTCn'nnn_
Cuts at: 223 911 1007 1959 2165 2348 4460 4670 223
Size: 688 96 952 206 183 2112 210 1990
Fragments arranged by size:
2112 1990 952 688 210 206 183 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4645 4955 5101 6247 4645
Size: 310 146 1146 4835
Fragments arranged by size:
4835 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3449 3449
Size: 6437
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 480 527 586 591 1537 1546 2353 2563
Size: 236 47 59 5 946 9 807 210
Cuts at: 2563 2764 2793 4262 4694 5441 5709 244
Size: 201 29 1469 432 747 268 972
Fragments arranged by size:
1469 972 946 807 747 432 268 236
210 201 59 47 29 9 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2981 2981
Size: 6437
EcoO109I rG'GnC_Cy
Cuts at: 1179 2511 2523 2766 2767 2820 3165 3399 3576
Size: 1332 12 243 1 53 345 234 177
Cuts at: 3576 1179
Size: 4040
Fragments arranged by size:
4040 1332 345 243 234 177 53 12
1
EcoRI G'AATT_C
Cuts at: 2662 3441 2662
Size: 779 5658
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4456 4456
Size: 6437
FspI TGC'GCA
Cuts at: 4218 4218
Size: 6437
HaeII r_GCGC'y
Cuts at: 764 1779 3871 4119 5141 6120 764
Size: 1015 2092 248 1022 979 1081
Fragments arranged by size:
2092 1081 1022 1015 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1563 1595 2486 2518 3042 3074 3115 3147 4279
Size: 32 891 32 524 32 41 32 1132
Cuts at: 4279 4309 4311 4341 1563
Size: 30 2 30 3659
Fragments arranged by size:
3659 1132 891 524 41 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 285 2094 3382 3476 285
Size: 1809 1288 94 3246
Fragments arranged by size:
3246 1809 1288 94
KasI G'GCGC_C
Cuts at: 760 1775 3867 4115 760
Size: 1015 2092 248 3082
Fragments arranged by size:
3082 2092 1015 248
KpnI G_GTAC'C
Cuts at: 3457 3457
Size: 6437
MluI A'CGCG_T
Cuts at: 5870 5870
Size: 6437
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 578 701 1579 5108 5292 578
Size: 123 878 3529 184 1723
Fragments arranged by size:
3529 1723 878 184 123
MscI TGG'CCA
Cuts at: 4198 4198
Size: 6437
MslI CAynn'nnrTG
Cuts at: 164 371 799 895 1126 1625 1834 1957 3079
Size: 207 428 96 231 499 209 123 1122
Cuts at: 3079 4426 4553 164
Size: 1347 127 2048
Fragments arranged by size:
2048 1347 1122 499 428 231 209 207
127 123 96
MspA1I CmG'CkG
Cuts at: 561 2118 3548 3862 4222 5235 5480 561
Size: 1557 1430 314 360 1013 245 1518
Fragments arranged by size:
1557 1518 1430 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1084 4618 1084
Size: 3534 2903
NarI GG'CG_CC
Cuts at: 761 1776 3868 4116 761
Size: 1015 2092 248 3082
Fragments arranged by size:
3082 2092 1015 248
NcoI C'CATG_G
Cuts at: 107 1229 3080 107
Size: 1122 1851 3464
Fragments arranged by size:
3464 1851 1122
NgoMIV G'CCGG_C
Cuts at: 1082 4616 1082
Size: 3534 2903
NsiI A_TGCA'T
Cuts at: 1520 1520
Size: 6437
NspI r_CATG'y
Cuts at: 1522 3490 3990 4897 1522
Size: 1968 500 907 3062
Fragments arranged by size:
3062 1968 907 500
PciI A'CATG_T
Cuts at: 4893 4893
Size: 6437
PflMI CCAn_nnn'nTGG
Cuts at: 310 664 1898 310
Size: 354 1234 4849
Fragments arranged by size:
4849 1234 354
PfoI T'CCnGG_A
Cuts at: 1493 3039 3167 1493
Size: 1546 128 4763
Fragments arranged by size:
4763 1546 128
PmeI GTTT'AAAC
Cuts at: 3436 3436
Size: 6437
PmlI CAC'GTG
Cuts at: 531 1128 531
Size: 597 5840
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 589 621 5576 5608 589
Size: 32 4955 32 1418
Fragments arranged by size:
4955 1418 32 32
PpuMI rG'GwC_Cy
Cuts at: 2820 3165 2820
Size: 345 6092
PshAI GACnn'nnGTC
Cuts at: 520 520
Size: 6437
PspOMI G'GGCC_C
Cuts at: 2511 2766 2511
Size: 255 6182
PspXI vC'TCGA_Gb
Cuts at: 2204 2204
Size: 6437
PstI C_TGCA'G
Cuts at: 551 771 1346 1760 2087 2858 3186 3484 4169
Size: 220 575 414 327 771 328 298 685
Cuts at: 4169 551
Size: 2819
Fragments arranged by size:
2819 771 685 575 414 328 327 298
220
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6437
PvuII CAG'CTG
Cuts at: 2118 3862 4222 2118
Size: 1744 360 4333
Fragments arranged by size:
4333 1744 360
RsrII CG'GwC_CG
Cuts at: 4632 4632
Size: 6437
SacI G_AGCT'C
Cuts at: 3451 3451
Size: 6437
SalI G'TCGA_C
Cuts at: 3380 3474 3380
Size: 94 6343
SapI GCTCTTCn'nnn_
Cuts at: 911 1959 2348 4460 4670 911
Size: 1048 389 2112 210 2678
Fragments arranged by size:
2678 2112 1048 389 210
SbfI CC_TGCA'GG
Cuts at: 3484 3484
Size: 6437
ScaI AGT'ACT
Cuts at: 1426 2589 5954 1426
Size: 1163 3365 1909
Fragments arranged by size:
3365 1909 1163
SfcI C'TryA_G
Cuts at: 33 547 767 1342 1756 2005 2083 2854 3182
Size: 514 220 575 414 249 78 771 328
Cuts at: 3182 3480 4165 5158 5349 33
Size: 298 685 993 191 1121
Fragments arranged by size:
1121 993 771 685 575 514 414 328
298 249 220 191 78
SfoI GGC'GCC
Cuts at: 762 1777 3869 4117 762
Size: 1015 2092 248 3082
Fragments arranged by size:
3082 2092 1015 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6437
SmaI CCC'GGG
Cuts at: 1162 1192 3459 1162
Size: 30 2267 4140
Fragments arranged by size:
4140 2267 30
SmlI C'TyrA_G
Cuts at: 2204 2833 3160 3592 4507 4999 5261 2204
Size: 629 327 432 915 492 262 3380
Fragments arranged by size:
3380 915 629 492 432 327 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6437
SphI G_CATG'C
Cuts at: 1522 3490 3990 1522
Size: 1968 500 3969
Fragments arranged by size:
3969 1968 500
SspI AAT'ATT
Cuts at: 1289 1933 1289
Size: 644 5793
StuI AGG'CCT
Cuts at: 571 1150 571
Size: 579 5858
StyI C'CwwG_G
Cuts at: 107 336 786 1175 1229 1605 2808 3080 3571
Size: 229 450 389 54 376 1203 272 491
Cuts at: 3571 3925 107
Size: 354 2619
Fragments arranged by size:
2619 1203 491 450 389 376 354 272
229 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1431 1516 2079 2375 3140 4020 4153 4612 4821
Size: 85 563 296 765 880 133 459 209
Cuts at: 4821 1431
Size: 3047
Fragments arranged by size:
3047 880 765 563 459 296 209 133
85
TatI w'GTAC_w
Cuts at: 392 1424 2480 2587 3071 5952 392
Size: 1032 1056 107 484 2881 877
Fragments arranged by size:
2881 1056 1032 877 484 107
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 449 481 1084 1116 1489 1521 5954 5986 449
Size: 32 603 32 373 32 4433 32 900
Fragments arranged by size:
4433 900 603 373 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2413 4234 2413
Size: 1821 4616
XbaI T'CTAG_A
Cuts at: 3468 3468
Size: 6437
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 561 684 2812 3932 561
Size: 123 2128 1120 3066
Fragments arranged by size:
3066 2128 1120 123
XhoI C'TCGA_G
Cuts at: 2204 2204
Size: 6437
XmaI C'CCGG_G
Cuts at: 1160 1190 3457 1160
Size: 30 2267 4140
Fragments arranged by size:
4140 2267 30
XmnI GAAnn'nnTTC
Cuts at: 1391 1391
Size: 6437
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AscI AvrII BaeI BbvCI BcgI
BmtI BsiWI BsmI BsmBI BssHII BstEII BstZ17I ClaI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PasI PsiI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI ZraI