Restriction map for
pF1KB9075
(Circular) (Six-base) MAPSORT of: pF1KB9075.seq Check: 495 from: 1 to: 7127
pF1KB9075 7127 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1122 1122
Size: 7127
AccI GT'mk_AC
Cuts at: 504 2030 4165 504
Size: 1526 2135 3466
Fragments arranged by size:
3466 2135 1526
Acc65I G'GTAC_C
Cuts at: 4143 4143
Size: 7127
AclI AA'CG_TT
Cuts at: 6967 6967
Size: 7127
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 575 1316 3730 3892 4952 5384 5737 6399 575
Size: 741 2414 162 1060 432 353 662 1303
Fragments arranged by size:
2414 1303 1060 741 662 432 353 162
AfeI AGC'GCT
Cuts at: 3877 3877
Size: 7127
AflIII A'CryG_T
Cuts at: 3350 4479 6279 6560 3350
Size: 1129 1800 281 3917
Fragments arranged by size:
3917 1800 1129 281
AhdI GACnn_n'nnGTC
Cuts at: 3354 3354
Size: 7127
AleI CACnn'nnGTG
Cuts at: 1081 1768 2404 2667 1081
Size: 687 636 263 5541
Fragments arranged by size:
5541 687 636 263
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2615 2647 2615
Size: 32 7095
AlwNI CAG_nnn'CTG
Cuts at: 119 252 472 3983 4432 5870 119
Size: 133 220 3511 449 1438 1376
Fragments arranged by size:
3511 1438 1376 449 220 133
ApaLI G'TGCA_C
Cuts at: 5965 5965
Size: 7127
ApoI r'AATT_y
Cuts at: 1691 4131 6480 6736 1691
Size: 2440 2349 256 2082
Fragments arranged by size:
2440 2349 2082 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7127
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7127
AvaI C'yCGr_G
Cuts at: 2170 2576 3239 4147 2170
Size: 406 663 908 5150
Fragments arranged by size:
5150 908 663 406
BamHI G'GATC_C
Cuts at: 4152 4152
Size: 7127
BanI G'GyrC_C
Cuts at: 220 1511 2002 4143 4557 4805 4840 220
Size: 1291 491 2141 414 248 35 2507
Fragments arranged by size:
2507 2141 1291 491 414 248 35
BanII G_rGCy'C
Cuts at: 569 2323 3013 3086 3471 4141 5171 569
Size: 1754 690 73 385 670 1030 2525
Fragments arranged by size:
2525 1754 1030 690 670 385 73
BbeI G_GCGC'C
Cuts at: 4561 4809 4561
Size: 248 6879
BbsI GAAGACnn'nnnn_
Cuts at: 2456 3140 2456
Size: 684 6443
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1178 1212 2986 3020 1178
Size: 34 1774 34 5285
Fragments arranged by size:
5285 1774 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1492 5025 6081 7127 1492
Size: 3533 1056 1046 1492
Fragments arranged by size:
3533 1492 1056 1046
BclI T'GATC_A
Cuts at: 2112 2384 4647 2112
Size: 272 2263 4592
Fragments arranged by size:
4592 2263 272
BglII A'GATC_T
Cuts at: 3099 4642 6679 3099
Size: 1543 2037 3547
Fragments arranged by size:
3547 2037 1543
BlpI GC'TnA_GC
Cuts at: 151 328 1327 4043 4239 151
Size: 177 999 2716 196 3039
Fragments arranged by size:
3039 2716 999 196 177
Bme1580I G_kGCm'C
Cuts at: 4004 4752 4845 5969 4004
Size: 748 93 1124 5162
Fragments arranged by size:
5162 1124 748 93
BmgBI CAC'GTC
Cuts at: 3351 4005 3351
Size: 654 6473
BmrI ACTGGGnnnn_n'
Cuts at: 129 817 1453 2602 3971 4339 4420 4755 6899
Size: 688 636 1149 1369 368 81 335 2144
Cuts at: 6899 129
Size: 357
Fragments arranged by size:
2144 1369 1149 688 636 368 357 335
81
BmtI G_CTAG'C
Cuts at: 1667 1667
Size: 7127
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1640 5494 1640
Size: 3854 3273
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 924 2035 2398 924
Size: 1111 363 5653
Fragments arranged by size:
5653 1111 363
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 383 2854 3898 3945 4303 5182 5896 6194 383
Size: 2471 1044 47 358 879 714 298 1316
Fragments arranged by size:
2471 1316 1044 879 714 358 298 47
BsaI GGTCTCn'nnnn_
Cuts at: 3404 3518 6831 3404
Size: 114 3313 3700
Fragments arranged by size:
3700 3313 114
BsaAI yAC'GTr
Cuts at: 2669 4480 2669
Size: 1811 5316
BsaBI GATnn'nnATC
Cuts at: 6 4641 6678 6
Size: 4635 2037 455
Fragments arranged by size:
4635 2037 455
BsaHI Gr'CG_yC
Cuts at: 4558 4806 5508 7004 4558
Size: 248 702 1496 4681
Fragments arranged by size:
4681 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4316 4837 5515 5926 6073 6347 4316
Size: 521 678 411 147 274 5096
Fragments arranged by size:
5096 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 242 272 2078 2108 3130 3160 242
Size: 30 1806 30 1022 30 4209
Fragments arranged by size:
4209 1806 1022 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 3070 3070
Size: 7127
BseYI C'CCAG_C
Cuts at: 224 2790 4552 5310 5975 6383 224
Size: 2566 1762 758 665 408 968
Fragments arranged by size:
2566 1762 968 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2784 2784
Size: 7127
BsiEI CG_ry'CG
Cuts at: 104 4669 4715 5945 104
Size: 4565 46 1230 1286
Fragments arranged by size:
4565 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 569 1952 3013 3471 4141 4919 5109 5969 569
Size: 1383 1061 458 670 778 190 860 1727
Fragments arranged by size:
1727 1383 1061 860 778 670 458 190
BsmI GAATG_Cn'
Cuts at: 1102 3161 3813 1102
Size: 2059 652 4416
Fragments arranged by size:
4416 2059 652
BsmBI CGTCTCn'nnnn_
Cuts at: 1856 1856
Size: 7127
Bsp1286I G_dGCh'C
Cuts at: 569 1952 2323 3013 3086 3471 4004 4141 4752
Size: 1383 371 690 73 385 533 137 611
Cuts at: 4752 4845 4919 5109 5171 5969 569
Size: 93 74 190 62 798 1727
Fragments arranged by size:
1727 1383 798 690 611 533 385 371
190 137 93 74 73 62
BspHI T'CATG_A
Cuts at: 1976 3764 5559 1976
Size: 1788 1795 3544
Fragments arranged by size:
3544 1795 1788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1122 1239 2795 4013 4177 4391 4693 5074 1122
Size: 117 1556 1218 164 214 302 381 3175
Fragments arranged by size:
3175 1556 1218 381 302 214 164 117
BsrBI CCG'CTC
Cuts at: 50 2541 5419 5473 6957 7122 50
Size: 2491 2878 54 1484 165 55
Fragments arranged by size:
2878 2491 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 5039 5039
Size: 7127
BsrFI r'CCGG_y
Cuts at: 1166 5125 5306 1166
Size: 3959 181 2987
Fragments arranged by size:
3959 2987 181
BsrGI T'GTAC_A
Cuts at: 521 3591 521
Size: 3070 4057
BssHII G'CGCG_C
Cuts at: 143 143
Size: 7127
BssSI C'ACGA_G
Cuts at: 885 1262 5398 6106 885
Size: 377 4136 708 1906
Fragments arranged by size:
4136 1906 708 377
BstAPI GCAn_nnn'nTGC
Cuts at: 234 4504 234
Size: 4270 2857
BstBI TT'CG_AA
Cuts at: 5488 5488
Size: 7127
BstEII G'GTnAC_C
Cuts at: 1226 1226
Size: 7127
BstXI CCAn_nnnn'nTGG
Cuts at: 3093 3135 3093
Size: 42 7085
BstYI r'GATC_y
Cuts at: 1 172 198 3099 4152 4619 4642 4977 5223
Size: 171 26 2901 1053 467 23 335 246
Cuts at: 5223 5529 5541 5627 6679 1
Size: 306 12 86 1052 449
Fragments arranged by size:
2901 1053 1052 467 449 335 306 246
171 86 26 23 12
BstZ17I GTA'TAC
Cuts at: 2031 2031
Size: 7127
Bsu36I CC'TnA_GG
Cuts at: 3390 3445 3390
Size: 55 7072
BtgI C'CryG_G
Cuts at: 107 2708 107
Size: 2601 4526
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5077 5258 5421 5077
Size: 181 163 6783
Fragments arranged by size:
6783 181 163
BtsI GCAGTG_nn'
Cuts at: 250 1361 2295 2539 4370 250
Size: 1111 934 244 1831 3007
Fragments arranged by size:
3007 1831 1111 934 244
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1356 1391 1356
Size: 35 7092
DraI TTT'AAA
Cuts at: 215 614 1353 2193 4126 215
Size: 399 739 840 1933 3216
Fragments arranged by size:
3216 1933 840 739 399
DraIII CAC_nnn'GTG
Cuts at: 2121 2121
Size: 7127
DrdI GACnn_nn'nnGTC
Cuts at: 130 4833 6177 130
Size: 4703 1344 1080
Fragments arranged by size:
4703 1344 1080
EaeI y'GGCC_r
Cuts at: 1934 2123 2711 2763 3125 4712 4886 5277 5304
Size: 189 588 52 362 1587 174 391 27
Cuts at: 5304 6649 1934
Size: 1345 2412
Fragments arranged by size:
2412 1587 1345 588 391 362 189 174
52 27
EagI C'GGCC_G
Cuts at: 4712 4712
Size: 7127
EarI CTCTTCn'nnn_
Cuts at: 1292 1562 2270 2425 2604 3047 5150 5360 1292
Size: 270 708 155 179 443 2103 210 3059
Fragments arranged by size:
3059 2103 708 443 270 210 179 155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5335 6077 6223 6937 5335
Size: 742 146 714 5525
Fragments arranged by size:
5525 742 714 146
EcoICRI GAG'CTC
Cuts at: 567 3011 3469 4139 567
Size: 2444 458 670 3555
Fragments arranged by size:
3555 2444 670 458
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 575 924 1316 2035 2398 3730 3892 4952 5384
Size: 349 392 719 363 1332 162 1060 432
Cuts at: 5384 5737 6399 575
Size: 353 662 1303
Fragments arranged by size:
1332 1303 1060 719 662 432 392 363
353 349 162
EcoO109I rG'GnC_Cy
Cuts at: 132 1821 2815 3171 4266 132
Size: 1689 994 356 1095 2993
Fragments arranged by size:
2993 1689 1095 994 356
EcoRI G'AATT_C
Cuts at: 4131 4131
Size: 7127
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2483 2633 3939 5146 2483
Size: 150 1306 1207 4464
Fragments arranged by size:
4464 1306 1207 150
FspI TGC'GCA
Cuts at: 4908 4908
Size: 7127
HaeII r_GCGC'y
Cuts at: 3879 4561 4809 6039 6810 3879
Size: 682 248 1230 771 4196
Fragments arranged by size:
4196 1230 771 682 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 240 272 750 782 2476 2508 3130 3162 3340
Size: 32 478 32 1694 32 622 32 178
Cuts at: 3340 3372 3453 3485 4075 4107 4969 4999 5001
Size: 32 81 32 590 32 862 30 2
Cuts at: 5001 5031 240
Size: 30 2336
Fragments arranged by size:
2336 1694 862 622 590 478 178 81
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1074 1791 1845 2784 4166 1074
Size: 717 54 939 1382 4035
Fragments arranged by size:
4035 1382 939 717 54
HindIII A'AGCT_T
Cuts at: 1752 3000 1752
Size: 1248 5879
HpaI GTT'AAC
Cuts at: 1074 1791 1845 2784 1074
Size: 717 54 939 5417
Fragments arranged by size:
5417 939 717 54
KasI G'GCGC_C
Cuts at: 4557 4805 4557
Size: 248 6879
KpnI G_GTAC'C
Cuts at: 4147 4147
Size: 7127
MfeI C'AATT_G
Cuts at: 858 858
Size: 7127
MluI A'CGCG_T
Cuts at: 6560 6560
Size: 7127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2885 5886 6070 2885
Size: 3001 184 3942
Fragments arranged by size:
3942 3001 184
MscI TGG'CCA
Cuts at: 1936 2125 2765 3127 4888 1936
Size: 189 640 362 1761 4175
Fragments arranged by size:
4175 1761 640 362 189
MslI CAynn'nnrTG
Cuts at: 1081 1091 1111 1768 2159 2404 2667 3091 3460
Size: 10 20 657 391 245 263 424 369
Cuts at: 3460 3817 5116 5243 1081
Size: 357 1299 127 2965
Fragments arranged by size:
2965 1299 657 424 391 369 357 263
245 127 20 10
MspA1I CmG'CkG
Cuts at: 155 425 3995 4238 4552 4912 5696 5941 155
Size: 270 3570 243 314 360 784 245 1341
Fragments arranged by size:
3570 1341 784 360 314 270 245 243
NaeI GCC'GGC
Cuts at: 5308 5308
Size: 7127
NarI GG'CG_CC
Cuts at: 4558 4806 4558
Size: 248 6879
NcoI C'CATG_G
Cuts at: 107 107
Size: 7127
NdeI CA'TA_TG
Cuts at: 1476 1476
Size: 7127
NgoMIV G'CCGG_C
Cuts at: 5306 5306
Size: 7127
NheI G'CTAG_C
Cuts at: 1663 1663
Size: 7127
NspI r_CATG'y
Cuts at: 2399 4180 4680 6283 2399
Size: 1781 500 1603 3243
Fragments arranged by size:
3243 1781 1603 500
PciI A'CATG_T
Cuts at: 6279 6279
Size: 7127
PflMI CCAn_nnn'nTGG
Cuts at: 2564 2564
Size: 7127
PfoI T'CCnGG_A
Cuts at: 1580 1580
Size: 7127
PmeI GTTT'AAAC
Cuts at: 4126 4126
Size: 7127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5573 5605 5573
Size: 32 7095
PpuMI rG'GwC_Cy
Cuts at: 132 1821 3171 132
Size: 1689 1350 4088
Fragments arranged by size:
4088 1689 1350
PsiI TTA'TAA
Cuts at: 2500 3251 2500
Size: 751 6376
PstI C_TGCA'G
Cuts at: 376 2017 4174 4859 376
Size: 1641 2157 685 2644
Fragments arranged by size:
2644 2157 1641 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7127
PvuII CAG'CTG
Cuts at: 155 425 3995 4552 4912 155
Size: 270 3570 557 360 2370
Fragments arranged by size:
3570 2370 557 360 270
RsrII CG'GwC_CG
Cuts at: 5322 5322
Size: 7127
SacI G_AGCT'C
Cuts at: 569 3013 3471 4141 569
Size: 2444 458 670 3555
Fragments arranged by size:
3555 2444 670 458
SalI G'TCGA_C
Cuts at: 4164 4164
Size: 7127
SapI GCTCTTCn'nnn_
Cuts at: 5150 5360 5150
Size: 210 6917
SbfI CC_TGCA'GG
Cuts at: 4174 4174
Size: 7127
ScaI AGT'ACT
Cuts at: 378 3478 6644 378
Size: 3100 3166 861
Fragments arranged by size:
3166 3100 861
SfcI C'TryA_G
Cuts at: 33 372 420 984 1911 2013 3333 4170 4855
Size: 339 48 564 927 102 1320 837 685
Cuts at: 4855 5823 6014 33
Size: 968 191 1146
Fragments arranged by size:
1320 1146 968 927 837 685 564 339
191 102 48
SfoI GGC'GCC
Cuts at: 4559 4807 4559
Size: 248 6879
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7127
SmaI CCC'GGG
Cuts at: 2578 3241 4149 2578
Size: 663 908 5556
Fragments arranged by size:
5556 908 663
SmlI C'TyrA_G
Cuts at: 398 2869 3913 3924 4282 5197 5911 6173 398
Size: 2471 1044 11 358 915 714 262 1352
Fragments arranged by size:
2471 1352 1044 915 714 358 262 11
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7127
SphI G_CATG'C
Cuts at: 4180 4680 4180
Size: 500 6627
StuI AGG'CCT
Cuts at: 2951 3907 2951
Size: 956 6171
StyI C'CwwG_G
Cuts at: 107 1670 3902 4261 4615 107
Size: 1563 2232 359 354 2619
Fragments arranged by size:
2619 2232 1563 359 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 238 4131 4710 4843 5302 5511 238
Size: 3893 579 133 459 209 1854
Fragments arranged by size:
3893 1854 579 459 209 133
TatI w'GTAC_w
Cuts at: 376 521 862 2720 3414 3476 3591 4103 6642
Size: 145 341 1858 694 62 115 512 2539
Cuts at: 6642 376
Size: 861
Fragments arranged by size:
2539 1858 861 694 512 341 145 115
62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 242 274 2078 2110 2518 2550 6644 6676 242
Size: 32 1804 32 408 32 4094 32 693
Fragments arranged by size:
4094 1804 693 408 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4924 4924
Size: 7127
XbaI T'CTAG_A
Cuts at: 1687 4158 1687
Size: 2471 4656
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3994 4622 3994
Size: 628 6499
XmaI C'CCGG_G
Cuts at: 2576 3239 4147 2576
Size: 663 908 5556
Fragments arranged by size:
5556 908 663
XmnI GAAnn'nnTTC
Cuts at: 3877 3877
Size: 7127
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AlfI ApaI AscI AvrII BaeI
BbvCI BfrBI BglI Bpu10I BsiWI BspEI ClaI EcoNI
EcoRV FseI FspAI NotI NruI NsiI PacI PasI
PmlI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI