Restriction map for
pF1KB9027
(Circular) (Six-base) MAPSORT of: pF1KB9027.seq Check: 9695 from: 1 to: 3830
pF1KB9027 3830 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 868 868
Size: 3830
Acc65I G'GTAC_C
Cuts at: 846 846
Size: 3830
AclI AA'CG_TT
Cuts at: 3670 3670
Size: 3830
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1655 2087 2440 3102 1655
Size: 432 353 662 2383
Fragments arranged by size:
2383 662 432 353
AflIII A'CryG_T
Cuts at: 1182 2982 3263 1182
Size: 1800 281 1749
Fragments arranged by size:
1800 1749 281
AgeI A'CCGG_T
Cuts at: 150 150
Size: 3830
AlwNI CAG_nnn'CTG
Cuts at: 1135 2573 1135
Size: 1438 2392
ApaLI G'TGCA_C
Cuts at: 2668 2668
Size: 3830
ApoI r'AATT_y
Cuts at: 698 834 3183 3439 698
Size: 136 2349 256 1089
Fragments arranged by size:
2349 1089 256 136
AseI AT'TA_AT
Cuts at: 20 247 277 20
Size: 227 30 3573
Fragments arranged by size:
3573 227 30
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3830
AvaI C'yCGr_G
Cuts at: 850 850
Size: 3830
BamHI G'GATC_C
Cuts at: 750 855 750
Size: 105 3725
BanI G'GyrC_C
Cuts at: 846 1260 1508 1543 846
Size: 414 248 35 3133
Fragments arranged by size:
3133 414 248 35
BanII G_rGCy'C
Cuts at: 844 1874 844
Size: 1030 2800
BbeI G_GCGC'C
Cuts at: 1264 1512 1264
Size: 248 3582
BciVI GTATCCnnnnn_n'
Cuts at: 1728 2784 3830 1728
Size: 1056 1046 1728
Fragments arranged by size:
1728 1056 1046
BclI T'GATC_A
Cuts at: 212 1350 212
Size: 1138 2692
BglII A'GATC_T
Cuts at: 1345 3382 1345
Size: 2037 1793
BlpI GC'TnA_GC
Cuts at: 942 942
Size: 3830
Bme1580I G_kGCm'C
Cuts at: 1455 1548 2672 1455
Size: 93 1124 2613
Fragments arranged by size:
2613 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1042 1123 1458 3602 1042
Size: 81 335 2144 1270
Fragments arranged by size:
2144 1270 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 3830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 151
Size: 3830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1006 1885 2599 2897 1006
Size: 879 714 298 1939
Fragments arranged by size:
1939 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3534 3534
Size: 3830
BsaAI yAC'GTr
Cuts at: 1183 1183
Size: 3830
BsaBI GATnn'nnATC
Cuts at: 6 749 1344 3381 6
Size: 743 595 2037 455
Fragments arranged by size:
2037 743 595 455
BsaHI Gr'CG_yC
Cuts at: 1261 1509 2211 3707 1261
Size: 248 702 1496 1384
Fragments arranged by size:
1496 1384 702 248
BsaWI w'CCGG_w
Cuts at: 150 1019 1540 2218 2629 2776 3050 150
Size: 869 521 678 411 147 274 930
Fragments arranged by size:
930 869 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1255 2013 2678 3086 1255
Size: 758 665 408 1999
Fragments arranged by size:
1999 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 220
Size: 3830
BsiEI CG_ry'CG
Cuts at: 104 1372 1418 2648 104
Size: 1268 46 1230 1286
Fragments arranged by size:
1286 1268 1230 46
BsiHKAI G_wGCw'C
Cuts at: 581 844 1622 1812 2672 581
Size: 263 778 190 860 1739
Fragments arranged by size:
1739 860 778 263 190
BsmI GAATG_Cn'
Cuts at: 362 362
Size: 3830
Bsp1286I G_dGCh'C
Cuts at: 581 844 1455 1548 1622 1812 1874 2672 581
Size: 263 611 93 74 190 62 798 1739
Fragments arranged by size:
1739 798 611 263 190 93 74 62
BspHI T'CATG_A
Cuts at: 2262 2262
Size: 3830
BspMI ACCTGCnnnn'nnnn_
Cuts at: 146 880 1094 1396 1777 146
Size: 734 214 302 381 2199
Fragments arranged by size:
2199 734 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2122 2176 3660 3825 50
Size: 2072 54 1484 165 55
Fragments arranged by size:
2072 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1742 1742
Size: 3830
BsrFI r'CCGG_y
Cuts at: 150 1828 2009 150
Size: 1678 181 1971
Fragments arranged by size:
1971 1678 181
BssSI C'ACGA_G
Cuts at: 2101 2809 2101
Size: 708 3122
BstAPI GCAn_nnn'nTGC
Cuts at: 1207 1207
Size: 3830
BstBI TT'CG_AA
Cuts at: 2191 2191
Size: 3830
BstXI CCAn_nnnn'nTGG
Cuts at: 817 817
Size: 3830
BstYI r'GATC_y
Cuts at: 1 143 750 855 1322 1345 1680 1926 2232
Size: 142 607 105 467 23 335 246 306
Cuts at: 2232 2244 2330 3382 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 607 467 449 335 306 246 142
105 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 773 773
Size: 3830
BtgI C'CryG_G
Cuts at: 107 107
Size: 3830
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1780 1961 2124 1780
Size: 181 163 3486
Fragments arranged by size:
3486 181 163
BtsI GCAGTG_nn'
Cuts at: 170 1073 170
Size: 903 2927
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 599 634 599
Size: 35 3795
DraI TTT'AAA
Cuts at: 255 829 255
Size: 574 3256
DrdI GACnn_nn'nnGTC
Cuts at: 1536 2880 1536
Size: 1344 2486
EaeI y'GGCC_r
Cuts at: 781 1415 1589 1980 2007 3352 781
Size: 634 174 391 27 1345 1259
Fragments arranged by size:
1345 1259 634 391 174 27
EagI C'GGCC_G
Cuts at: 1415 1415
Size: 3830
EarI CTCTTCn'nnn_
Cuts at: 1853 2063 1853
Size: 210 3620
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2038 2780 2926 3640 2038
Size: 742 146 714 2228
Fragments arranged by size:
2228 742 714 146
EcoICRI GAG'CTC
Cuts at: 842 842
Size: 3830
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 1655 2087 2440 3102 151
Size: 1504 432 353 662 879
Fragments arranged by size:
1504 879 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 558 969 558
Size: 411 3419
EcoRI G'AATT_C
Cuts at: 698 834 698
Size: 136 3694
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1849 1849
Size: 3830
FspI TGC'GCA
Cuts at: 1611 1611
Size: 3830
HaeII r_GCGC'y
Cuts at: 1264 1512 2742 3513 1264
Size: 248 1230 771 1581
Fragments arranged by size:
1581 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1672 1702 1704 1734 1672
Size: 30 2 30 3768
Fragments arranged by size:
3768 30 30 2
HincII GTy'rAC
Cuts at: 869 869
Size: 3830
KasI G'GCGC_C
Cuts at: 1260 1508 1260
Size: 248 3582
KpnI G_GTAC'C
Cuts at: 850 850
Size: 3830
MluI A'CGCG_T
Cuts at: 3263 3263
Size: 3830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 185 288 364 621 2589 2773 185
Size: 103 76 257 1968 184 1242
Fragments arranged by size:
1968 1242 257 184 103 76
MscI TGG'CCA
Cuts at: 1591 1591
Size: 3830
MslI CAynn'nnrTG
Cuts at: 1819 1946 1819
Size: 127 3703
MspA1I CmG'CkG
Cuts at: 813 941 1255 1615 2399 2644 813
Size: 128 314 360 784 245 1999
Fragments arranged by size:
1999 784 360 314 245 128
NaeI GCC'GGC
Cuts at: 2011 2011
Size: 3830
NarI GG'CG_CC
Cuts at: 1261 1509 1261
Size: 248 3582
NcoI C'CATG_G
Cuts at: 107 107
Size: 3830
NgoMIV G'CCGG_C
Cuts at: 2009 2009
Size: 3830
NspI r_CATG'y
Cuts at: 883 1383 2986 883
Size: 500 1603 1727
Fragments arranged by size:
1727 1603 500
PasI CC'CwG_GG
Cuts at: 572 572
Size: 3830
PciI A'CATG_T
Cuts at: 2982 2982
Size: 3830
PmeI GTTT'AAAC
Cuts at: 829 829
Size: 3830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2276 2308 2276
Size: 32 3798
PstI C_TGCA'G
Cuts at: 143 877 1562 143
Size: 734 685 2411
Fragments arranged by size:
2411 734 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3830
PvuII CAG'CTG
Cuts at: 1255 1615 1255
Size: 360 3470
RsrII CG'GwC_CG
Cuts at: 2025 2025
Size: 3830
SacI G_AGCT'C
Cuts at: 844 844
Size: 3830
SalI G'TCGA_C
Cuts at: 867 867
Size: 3830
SapI GCTCTTCn'nnn_
Cuts at: 1853 2063 1853
Size: 210 3620
SbfI CC_TGCA'GG
Cuts at: 877 877
Size: 3830
ScaI AGT'ACT
Cuts at: 3347 3347
Size: 3830
SexAI A'CCwGG_T
Cuts at: 684 684
Size: 3830
SfcI C'TryA_G
Cuts at: 33 139 873 1558 2526 2717 33
Size: 106 734 685 968 191 1146
Fragments arranged by size:
1146 968 734 685 191 106
SfoI GGC'GCC
Cuts at: 1262 1510 1262
Size: 248 3582
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3830
SmaI CCC'GGG
Cuts at: 852 852
Size: 3830
SmlI C'TyrA_G
Cuts at: 985 1900 2614 2876 985
Size: 915 714 262 1939
Fragments arranged by size:
1939 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3830
SphI G_CATG'C
Cuts at: 883 1383 883
Size: 500 3330
StuI AGG'CCT
Cuts at: 475 475
Size: 3830
StyI C'CwwG_G
Cuts at: 107 964 1318 107
Size: 857 354 2619
Fragments arranged by size:
2619 857 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 564 1413 1546 2005 2214 564
Size: 849 133 459 209 2180
Fragments arranged by size:
2180 849 459 209 133
TatI w'GTAC_w
Cuts at: 230 3345 230
Size: 3115 715
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3347 3379 3347
Size: 32 3798
Tth111I GACn'n_nGTC
Cuts at: 1627 1627
Size: 3830
XbaI T'CTAG_A
Cuts at: 861 861
Size: 3830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1325 1325
Size: 3830
XmaI C'CCGG_G
Cuts at: 850 850
Size: 3830
XmnI GAAnn'nnTTC
Cuts at: 535 535
Size: 3830
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmBI
BspEI BsrGI BssHII BstEII BstZ17I ClaI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI