Restriction map for
pF1KB9019
(Circular) (Six-base) MAPSORT of: pF1KB9019.seq Check: 5536 from: 1 to: 10487
pF1KB9019 10487 bp
With 182 enzymes: SgfI *
January 8, 2014 09:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 938 1854 4069 7457 938
Size: 916 2215 3388 3968
Fragments arranged by size:
3968 3388 2215 916
AatII G_ACGT'C
Cuts at: 3935 3935
Size: 10487
AccI GT'mk_AC
Cuts at: 667 6026 7456 7525 667
Size: 5359 1430 69 3629
Fragments arranged by size:
5359 3629 1430 69
Acc65I G'GTAC_C
Cuts at: 7503 7503
Size: 10487
AclI AA'CG_TT
Cuts at: 2912 10327 2912
Size: 7415 3072
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1154 1496 1975 2142 3190 4206 4287 6298 6643
Size: 342 479 167 1048 1016 81 2011 345
Cuts at: 6643 7345 8312 8744 9491 9759 1154
Size: 702 967 432 747 268 1882
Fragments arranged by size:
2011 1882 1048 1016 967 747 702 479
432 345 342 268 167 81
AflII C'TTAA_G
Cuts at: 1018 1018
Size: 10487
AflIII A'CryG_T
Cuts at: 2144 2761 3596 3863 5636 6605 7839 8943 9920
Size: 617 835 267 1773 969 1234 1104 977
Cuts at: 9920 2144
Size: 2711
Fragments arranged by size:
2711 1773 1234 1104 977 969 835 617
267
AgeI A'CCGG_T
Cuts at: 8875 8875
Size: 10487
AhdI GACnn_n'nnGTC
Cuts at: 476 476
Size: 10487
AleI CACnn'nnGTG
Cuts at: 3328 4063 4858 3328
Size: 735 795 8957
Fragments arranged by size:
8957 795 735
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2207 2207
Size: 10487
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2111 2143 2267 2299 4708 4740 4828 4860 6805
Size: 32 124 32 2409 32 88 32 1945
Cuts at: 6805 6837 2111
Size: 32 5761
Fragments arranged by size:
5761 2409 1945 124 88 32 32 32
32 32
AlwNI CAG_nnn'CTG
Cuts at: 259 2121 2604 3060 3609 3789 5241 6433 6442
Size: 1862 483 456 549 180 1452 1192 9
Cuts at: 6442 6621 7260 7792 9359 259
Size: 179 639 532 1567 1387
Fragments arranged by size:
1862 1567 1452 1387 1192 639 549 532
483 456 180 179 9
ApaI G_GGCC'C
Cuts at: 702 1079 5165 702
Size: 377 4086 6024
Fragments arranged by size:
6024 4086 377
ApaLI G'TGCA_C
Cuts at: 9257 9257
Size: 10487
ApoI r'AATT_y
Cuts at: 2124 4072 4248 4516 5579 5967 6211 7491 9840
Size: 1948 176 268 1063 388 244 1280 2349
Cuts at: 9840 10096 2124
Size: 256 2515
Fragments arranged by size:
2515 2349 1948 1280 1063 388 268 256
244 176
AseI AT'TA_AT
Cuts at: 20 2557 3904 20
Size: 2537 1347 6603
Fragments arranged by size:
6603 2537 1347
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10487
AvaI C'yCGr_G
Cuts at: 234 1009 2039 3886 5558 7375 7507 234
Size: 775 1030 1847 1672 1817 132 3214
Fragments arranged by size:
3214 1847 1817 1672 1030 775 132
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3507 3540 3507
Size: 33 10454
BamHI G'GATC_C
Cuts at: 3470 5506 7512 3470
Size: 2036 2006 6445
Fragments arranged by size:
6445 2036 2006
BanI G'GyrC_C
Cuts at: 2953 3206 4057 4422 5602 6333 7503 7917 8165
Size: 253 851 365 1180 731 1170 414 248
Cuts at: 8165 8200 2953
Size: 35 5240
Fragments arranged by size:
5240 1180 1170 851 731 414 365 253
248 35
BanII G_rGCy'C
Cuts at: 248 613 702 1079 2104 2678 2996 3152 3734
Size: 365 89 377 1025 574 318 156 582
Cuts at: 3734 3780 4166 4364 5165 5308 5839 6231 6704
Size: 46 386 198 801 143 531 392 473
Cuts at: 6704 7501 8531 248
Size: 797 1030 2204
Fragments arranged by size:
2204 1030 1025 801 797 582 574 531
473 392 386 377 365 318 198 156
143 89 46
BbeI G_GCGC'C
Cuts at: 5606 7921 8169 5606
Size: 2315 248 7924
Fragments arranged by size:
7924 2315 248
BbsI GAAGACnn'nnnn_
Cuts at: 563 3084 3408 5672 7072 563
Size: 2521 324 2264 1400 3978
Fragments arranged by size:
3978 2521 2264 1400 324
BbvCI CC'TCA_GC
Cuts at: 513 4147 4997 513
Size: 3634 850 6003
Fragments arranged by size:
6003 3634 850
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2632 2666 2632
Size: 34 10453
BciVI GTATCCnnnnn_n'
Cuts at: 5330 8385 9146 10487 5330
Size: 3055 761 1341 5330
Fragments arranged by size:
5330 3055 1341 761
BclI T'GATC_A
Cuts at: 1463 2897 3659 4760 5180 5783 8007 1463
Size: 1434 762 1101 420 603 2224 3943
Fragments arranged by size:
3943 2224 1434 1101 762 603 420
BfrBI ATG'CAT
Cuts at: 6066 6066
Size: 10487
BglI GCCn_nnn'nGGC
Cuts at: 1246 1729 1246
Size: 483 10004
BglII A'GATC_T
Cuts at: 5119 6834 8002 10039 5119
Size: 1715 1168 2037 5567
Fragments arranged by size:
5567 2037 1715 1168
BlpI GC'TnA_GC
Cuts at: 3742 7427 7599 3742
Size: 3685 172 6630
Fragments arranged by size:
6630 3685 172
Bme1580I G_kGCm'C
Cuts at: 702 1079 2213 4121 4286 4649 4901 5165 8112
Size: 377 1134 1908 165 363 252 264 2947
Cuts at: 8112 8205 9261 702
Size: 93 1056 1928
Fragments arranged by size:
2947 1928 1908 1134 1056 377 363 264
252 165 93
BmgBI CAC'GTC
Cuts at: 2049 2762 2049
Size: 713 9774
BmrI ACTGGGnnnn_n'
Cuts at: 476 1226 3300 7699 7780 8115 10259 476
Size: 750 2074 4399 81 335 2144 704
Fragments arranged by size:
4399 2144 2074 750 704 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 354 3732 8854 354
Size: 3378 5122 1987
Fragments arranged by size:
5122 3378 1987
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 730 1332 2195 2350 3010 3247 4426 4837
Size: 228 602 863 155 660 237 1179 411
Cuts at: 4837 4855 5100 5197 5540 6148 6597 6952 7051
Size: 18 245 97 343 608 449 355 99
Cuts at: 7051 7078 7132 502
Size: 27 54 3857
Fragments arranged by size:
3857 1179 863 660 608 602 449 411
355 343 245 237 228 155 99 97
54 27 18
Bpu10I CC'TnA_GC
Cuts at: 513 4147 4997 5838 7405 513
Size: 3634 850 841 1567 3595
Fragments arranged by size:
3634 3595 1567 850 841
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2569 3880 7327 7663 8542 9034 9332 2569
Size: 1311 3447 336 879 492 298 3724
Fragments arranged by size:
3724 3447 1311 879 492 336 298
BsaI GGTCTCn'nnnn_
Cuts at: 733 2326 10191 733
Size: 1593 7865 1029
Fragments arranged by size:
7865 1593 1029
BsaAI yAC'GTr
Cuts at: 3996 5586 5646 7164 7319 7840 3996
Size: 1590 60 1518 155 521 6643
Fragments arranged by size:
6643 1590 1518 521 155 60
BsaBI GATnn'nnATC
Cuts at: 6 1901 3718 5011 5179 5476 6682 8001 10038
Size: 1895 1817 1293 168 297 1206 1319 2037
Cuts at: 10038 6
Size: 455
Fragments arranged by size:
2037 1895 1817 1319 1293 1206 455 297
168
BsaHI Gr'CG_yC
Cuts at: 3932 3979 5603 7040 7092 7918 8166 8868 10364
Size: 47 1624 1437 52 826 248 702 1496
Cuts at: 10364 3932
Size: 4055
Fragments arranged by size:
4055 1624 1496 1437 826 702 248 52
47
BsaWI w'CCGG_w
Cuts at: 986 1188 1499 2129 2412 4106 4616 6589 7676
Size: 202 311 630 283 1694 510 1973 1087
Cuts at: 7676 8197 8875 9149 9296 9707 986
Size: 521 678 274 147 411 1766
Fragments arranged by size:
1973 1766 1694 1087 678 630 521 510
411 311 283 274 202 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 613 643 1183 1213 2203 2233 3793 3823 4199
Size: 30 540 30 990 30 1560 30 376
Cuts at: 4199 4229 4828 4858 6121 6151 6805 6835 613
Size: 30 599 30 1263 30 654 30 4265
Fragments arranged by size:
4265 1560 1263 990 654 599 540 376
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 501 618 4945 5638 5780 7096 501
Size: 117 4327 693 142 1316 3892
Fragments arranged by size:
4327 3892 1316 693 142 117
BseYI C'CCAG_C
Cuts at: 319 1157 1592 1916 2520 2812 2860 3050 3346
Size: 838 435 324 604 292 48 190 296
Cuts at: 3346 5165 5851 5940 6045 7422 7912 8670 9247
Size: 1819 686 89 105 1377 490 758 577
Cuts at: 9247 9743 319
Size: 496 1063
Fragments arranged by size:
1819 1377 1063 838 758 686 604 577
496 490 435 324 296 292 190 105
89 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 1159 1814 1863 1930 2167 2419 3067 3202
Size: 354 655 49 67 237 252 648 135
Cuts at: 3202 3463 4415 7012 7273 805
Size: 261 952 2597 261 4019
Fragments arranged by size:
4019 2597 952 655 648 354 261 261
252 237 135 67 49
BsiEI CG_ry'CG
Cuts at: 104 366 1913 7023 8029 8075 9283 104
Size: 262 1547 5110 1006 46 1208 1308
Fragments arranged by size:
5110 1547 1308 1208 1006 262 46
BsiHKAI G_wGCw'C
Cuts at: 2104 2996 3533 3734 3758 4166 6000 6231 6704
Size: 892 537 201 24 408 1834 231 473
Cuts at: 6704 7501 8279 8469 9261 2104
Size: 797 778 190 792 3330
Fragments arranged by size:
3330 1834 892 797 792 778 537 473
408 231 201 190 24
BsmI GAATG_Cn'
Cuts at: 1647 5570 1647
Size: 3923 6564
BsmBI CGTCTCn'nnnn_
Cuts at: 7195 7195
Size: 10487
Bsp1286I G_dGCh'C
Cuts at: 248 613 702 1079 2104 2213 2678 2996 3152
Size: 365 89 377 1025 109 465 318 156
Cuts at: 3152 3533 3734 3758 3780 4121 4166 4286 4364
Size: 381 201 24 22 341 45 120 78
Cuts at: 4364 4649 4901 5165 5308 5839 6000 6231 6704
Size: 285 252 264 143 531 161 231 473
Cuts at: 6704 7501 8112 8205 8279 8469 8531 9261 248
Size: 797 611 93 74 190 62 730 1474
Fragments arranged by size:
1474 1025 797 730 611 531 473 465
381 377 365 341 318 285 264 252
231 201 190 161 156 143 120 109
93 89 78 74 62 45 24 22
BspEI T'CCGG_A
Cuts at: 1188 1499 2129 9707 1188
Size: 311 630 7578 1968
Fragments arranged by size:
7578 1968 630 311
BspHI T'CATG_A
Cuts at: 1580 2624 2900 3125 3191 3242 9663 1580
Size: 1044 276 225 66 51 6421 2404
Fragments arranged by size:
6421 2404 1044 276 225 66 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 827 938 1854 4069 4151 4596 4796 5546 5911
Size: 111 916 2215 82 445 200 750 365
Cuts at: 5911 6968 7428 7457 7537 7751 8053 8434 827
Size: 1057 460 29 80 214 302 381 2880
Fragments arranged by size:
2880 2215 1057 916 750 460 445 381
365 302 214 200 111 82 80 29
BsrBI CCG'CTC
Cuts at: 50 978 4806 6296 7431 8779 8833 10317 10482
Size: 928 3828 1490 1135 1348 54 1484 165
Cuts at: 10482 50
Size: 55
Fragments arranged by size:
3828 1490 1484 1348 1135 928 165 55
54
BsrDI GCAATG_nn'
Cuts at: 1118 2051 2384 5490 8399 1118
Size: 933 333 3106 2909 3206
Fragments arranged by size:
3206 3106 2909 933 333
BsrFI r'CCGG_y
Cuts at: 361 1710 1908 1949 2657 4040 4967 5029 6938
Size: 1349 198 41 708 1383 927 62 1909
Cuts at: 6938 8485 8666 8875 361
Size: 1547 181 209 1973
Fragments arranged by size:
1973 1909 1547 1383 1349 927 708 209
198 181 62 41
BssSI C'ACGA_G
Cuts at: 659 2185 8758 9116 659
Size: 1526 6573 358 2030
Fragments arranged by size:
6573 2030 1526 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3060 4871 5175 6433 6442 7864 3060
Size: 1811 304 1258 9 1422 5683
Fragments arranged by size:
5683 1811 1422 1258 304 9
BstBI TT'CG_AA
Cuts at: 2251 4781 8848 2251
Size: 2530 4067 3890
Fragments arranged by size:
4067 3890 2530
BstEII G'GTnAC_C
Cuts at: 1002 5898 1002
Size: 4896 5591
BstXI CCAn_nnnn'nTGG
Cuts at: 538 1767 1779 3369 5895 6049 538
Size: 1229 12 1590 2526 154 4976
Fragments arranged by size:
4976 2526 1590 1229 154 12
BstYI r'GATC_y
Cuts at: 1 1556 1619 2800 3470 3501 3785 5119 5205
Size: 1555 63 1181 670 31 284 1334 86
Cuts at: 5205 5506 6834 7133 7512 7979 8002 8337 8583
Size: 301 1328 299 379 467 23 335 246
Cuts at: 8583 9595 9681 9693 10039 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1555 1334 1328 1181 1012 670 467 449
379 346 335 301 299 284 246 86
86 63 31 23 12
Bsu36I CC'TnA_GG
Cuts at: 1251 3574 1251
Size: 2323 8164
BtgI C'CryG_G
Cuts at: 107 836 1778 1931 1987 2306 3474 4211 4823
Size: 729 942 153 56 319 1168 737 612
Cuts at: 4823 6309 6350 6386 7385 7460 107
Size: 1486 41 36 999 75 3134
Fragments arranged by size:
3134 1486 1168 999 942 737 729 612
319 153 75 56 41 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1704 2476 3996 8437 8618 8781 1704
Size: 772 1520 4441 181 163 3410
Fragments arranged by size:
4441 3410 1520 772 181 163
BtsI GCAGTG_nn'
Cuts at: 1759 1942 2211 3058 3214 3756 5040 7261 7730
Size: 183 269 847 156 542 1284 2221 469
Cuts at: 7730 1759
Size: 4516
Fragments arranged by size:
4516 2221 1284 847 542 469 269 183
156
ClaI AT'CG_AT
Cuts at: 3671 6860 3671
Size: 3189 7298
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 5632 5667 5632
Size: 35 10452
DraI TTT'AAA
Cuts at: 5295 5685 7486 5295
Size: 390 1801 8296
Fragments arranged by size:
8296 1801 390
DraIII CAC_nnn'GTG
Cuts at: 786 2369 6984 786
Size: 1583 4615 4289
Fragments arranged by size:
4615 4289 1583
DrdI GACnn_nn'nnGTC
Cuts at: 8193 9051 8193
Size: 858 9629
EaeI y'GGCC_r
Cuts at: 1712 1730 1769 1910 2165 2453 2981 3638 3870
Size: 18 39 141 255 288 528 657 232
Cuts at: 3870 7020 7382 8072 8246 8637 8664 10009 1712
Size: 3150 362 690 174 391 27 1345 2190
Fragments arranged by size:
3150 2190 1345 690 657 528 391 362
288 255 232 174 141 39 27 18
EagI C'GGCC_G
Cuts at: 1910 7020 8072 1910
Size: 5110 1052 4325
Fragments arranged by size:
5110 4325 1052
EarI CTCTTCn'nnn_
Cuts at: 784 2234 2717 4171 4306 4352 5870 6012 8510
Size: 1450 483 1454 135 46 1518 142 2498
Cuts at: 8510 8720 784
Size: 210 2551
Fragments arranged by size:
2551 2498 1518 1454 1450 483 210 142
135 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 384 1063 1499 1753 2419 5005 5038 8695 9005
Size: 679 436 254 666 2586 33 3657 310
Cuts at: 9005 9151 10297 384
Size: 146 1146 574
Fragments arranged by size:
3657 2586 1146 679 666 574 436 310
254 146 33
EcoICRI GAG'CTC
Cuts at: 2102 2994 3732 4164 6229 6702 7499 2102
Size: 892 738 432 2065 473 797 5090
Fragments arranged by size:
5090 2065 892 797 738 473 432
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 730 1154 1332 1496 1975 2142 2195 2350
Size: 228 424 178 164 479 167 53 155
Cuts at: 2350 3010 3190 3247 4206 4287 4426 4837 4855
Size: 660 180 57 959 81 139 411 18
Cuts at: 4855 5100 5197 5540 6148 6298 6597 6643 6952
Size: 245 97 343 608 150 299 46 309
Cuts at: 6952 7051 7078 7132 7345 8312 8744 9491 9759
Size: 99 27 54 213 967 432 747 268
Cuts at: 9759 502
Size: 1230
Fragments arranged by size:
1230 967 959 747 660 608 479 432
424 411 343 309 299 268 245 228
213 180 178 167 164 155 150 139
99 97 81 57 54 53 46 27
18
EcoNI CCTnn'n_nnAGG
Cuts at: 516 7111 516
Size: 6595 3892
EcoO109I rG'GnC_Cy
Cuts at: 349 521 698 699 1014 1075 1225 1296 2441
Size: 172 177 1 315 61 150 71 1145
Cuts at: 2441 2514 5161 5162 5526 5924 6009 6927 6957
Size: 73 2647 1 364 398 85 918 30
Cuts at: 6957 7626 349
Size: 669 3210
Fragments arranged by size:
3210 2647 1145 918 669 398 364 315
177 172 150 85 73 71 61 30
1 1
EcoRI G'AATT_C
Cuts at: 4072 5967 6211 7491 4072
Size: 1895 244 1280 7068
Fragments arranged by size:
7068 1895 1280 244
EcoRV GAT'ATC
Cuts at: 1962 2418 5793 1962
Size: 456 3375 6656
Fragments arranged by size:
6656 3375 456
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 7242 8506 7242
Size: 1264 9223
FspI TGC'GCA
Cuts at: 4864 8268 4864
Size: 3404 7083
FspAI rTGC'GCAy
Cuts at: 4864 4864
Size: 10487
HaeII r_GCGC'y
Cuts at: 5606 7921 8169 9191 10170 5606
Size: 2315 248 1022 979 5923
Fragments arranged by size:
5923 2315 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 462 494 610 613 625 642 645 657 1035
Size: 32 116 3 12 17 3 12 378
Cuts at: 1035 1067 1247 1279 2033 2065 5104 5136 5172
Size: 32 180 32 754 32 3039 32 36
Cuts at: 5172 5204 5768 5800 6121 6153 6623 6655 8329
Size: 32 564 32 321 32 470 32 1674
Cuts at: 8329 8359 8361 8391 462
Size: 30 2 30 2558
Fragments arranged by size:
3039 2558 1674 754 564 470 378 321
180 116 36 32 32 32 32 32
32 32 32 32 30 30 17 12
12 3 3 2
HincII GTy'rAC
Cuts at: 2730 7526 2730
Size: 4796 5691
HindIII A'AGCT_T
Cuts at: 1469 1469
Size: 10487
KasI G'GCGC_C
Cuts at: 5602 7917 8165 5602
Size: 2315 248 7924
Fragments arranged by size:
7924 2315 248
KpnI G_GTAC'C
Cuts at: 7507 7507
Size: 10487
MluI A'CGCG_T
Cuts at: 9920 9920
Size: 10487
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 656 2354 3479 3683 4506 5336 6059 6935 9158
Size: 1698 1125 204 823 830 723 876 2223
Cuts at: 9158 9342 656
Size: 184 1801
Fragments arranged by size:
2223 1801 1698 1125 876 830 823 723
204 184
MscI TGG'CCA
Cuts at: 1732 1771 2983 3640 8248 1732
Size: 39 1212 657 4608 3971
Fragments arranged by size:
4608 3971 1212 657 39
MslI CAynn'nnrTG
Cuts at: 1042 1885 1936 2260 2713 3328 3862 4063 4858
Size: 843 51 324 453 615 534 201 795
Cuts at: 4858 4951 6071 6091 6757 8476 8603 1042
Size: 93 1120 20 666 1719 127 2926
Fragments arranged by size:
2926 1719 1120 843 795 666 615 534
453 324 201 127 93 51 20
MspA1I CmG'CkG
Cuts at: 588 850 1680 1728 2524 3222 3396 3467 3476
Size: 262 830 48 796 698 174 71 9
Cuts at: 3476 3654 3741 4178 4605 5373 6045 6747 7148
Size: 178 87 437 427 768 672 702 401
Cuts at: 7148 7426 7598 7912 8272 9285 9530 588
Size: 278 172 314 360 1013 245 1545
Fragments arranged by size:
1545 1013 830 796 768 702 698 672
437 427 401 360 314 278 262 245
178 174 172 87 71 48 9
NaeI GCC'GGC
Cuts at: 1910 4969 6940 8668 1910
Size: 3059 1971 1728 3729
Fragments arranged by size:
3729 3059 1971 1728
NarI GG'CG_CC
Cuts at: 5603 7918 8166 5603
Size: 2315 248 7924
Fragments arranged by size:
7924 2315 248
NcoI C'CATG_G
Cuts at: 107 1931 1987 4211 4823 7460 107
Size: 1824 56 2224 612 2637 3134
Fragments arranged by size:
3134 2637 2224 1824 612 56
NgoMIV G'CCGG_C
Cuts at: 1908 4967 6938 8666 1908
Size: 3059 1971 1728 3729
Fragments arranged by size:
3729 3059 1971 1728
NruI TCG'CGA
Cuts at: 1664 6953 1664
Size: 5289 5198
NsiI A_TGCA'T
Cuts at: 6068 6068
Size: 10487
NspI r_CATG'y
Cuts at: 771 936 3600 3867 5640 6609 7540 8040 8947
Size: 165 2664 267 1773 969 931 500 907
Cuts at: 8947 771
Size: 2311
Fragments arranged by size:
2664 2311 1773 969 931 907 500 267
165
PasI CC'CwG_GG
Cuts at: 525 5241 6338 525
Size: 4716 1097 4674
Fragments arranged by size:
4716 4674 1097
PciI A'CATG_T
Cuts at: 3596 3863 5636 6605 8943 3596
Size: 267 1773 969 2338 5140
Fragments arranged by size:
5140 2338 1773 969 267
PflMI CCAn_nnn'nTGG
Cuts at: 1842 3609 4203 5241 5376 6984 1842
Size: 1767 594 1038 135 1608 5345
Fragments arranged by size:
5345 1767 1608 1038 594 135
PfoI T'CCnGG_A
Cuts at: 2104 2114 2327 3104 3368 4049 4814 5267 6125
Size: 10 213 777 264 681 765 453 858
Cuts at: 6125 7055 7109 2104
Size: 930 54 5482
Fragments arranged by size:
5482 930 858 777 765 681 453 264
213 54 10
PmeI GTTT'AAAC
Cuts at: 7486 7486
Size: 10487
PmlI CAC'GTG
Cuts at: 5646 7164 5646
Size: 1518 8969
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4828 4860 6805 6837 6890 6922 9626 9658 4828
Size: 32 1945 32 53 32 2704 32 5657
Fragments arranged by size:
5657 2704 1945 53 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1014 1296 2514 5526 6009 6927 6957 1014
Size: 282 1218 3012 483 918 30 4544
Fragments arranged by size:
4544 3012 1218 918 483 282 30
PshAI GACnn'nnGTC
Cuts at: 745 745
Size: 10487
PspOMI G'GGCC_C
Cuts at: 698 1075 5161 698
Size: 377 4086 6024
Fragments arranged by size:
6024 4086 377
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 7303 7335 7303
Size: 32 10455
PstI C_TGCA'G
Cuts at: 1358 3236 3395 4037 4793 6438 7142 7534 8219
Size: 1878 159 642 756 1645 704 392 685
Cuts at: 8219 1358
Size: 3626
Fragments arranged by size:
3626 1878 1645 756 704 685 642 392
159
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10487
PvuII CAG'CTG
Cuts at: 588 3654 3741 4605 5373 6045 7426 7912 8272
Size: 3066 87 864 768 672 1381 486 360
Cuts at: 8272 588
Size: 2803
Fragments arranged by size:
3066 2803 1381 864 768 672 486 360
87
RsrII CG'GwC_CG
Cuts at: 8682 8682
Size: 10487
SacI G_AGCT'C
Cuts at: 2104 2996 3734 4166 6231 6704 7501 2104
Size: 892 738 432 2065 473 797 5090
Fragments arranged by size:
5090 2065 892 797 738 473 432
SacII CC_GC'GG
Cuts at: 3477 3477
Size: 10487
SalI G'TCGA_C
Cuts at: 7524 7524
Size: 10487
SapI GCTCTTCn'nnn_
Cuts at: 4171 4352 8510 8720 4171
Size: 181 4158 210 5938
Fragments arranged by size:
5938 4158 210 181
SbfI CC_TGCA'GG
Cuts at: 7142 7534 7142
Size: 392 10095
ScaI AGT'ACT
Cuts at: 10004 10004
Size: 10487
SexAI A'CCwGG_T
Cuts at: 782 3086 3830 6614 6980 7118 7241 782
Size: 2304 744 2784 366 138 123 4028
Fragments arranged by size:
4028 2784 2304 744 366 138 123
SfcI C'TryA_G
Cuts at: 33 1291 1354 1815 1921 2789 3232 3391 4033
Size: 1258 63 461 106 868 443 159 642
Cuts at: 4033 4789 4990 6434 7138 7530 8215 9208 9399
Size: 756 201 1444 704 392 685 993 191
Cuts at: 9399 33
Size: 1121
Fragments arranged by size:
1444 1258 1121 993 868 756 704 685
642 461 443 392 201 191 159 106
63
SfiI GGCCn_nnn'nGGCC
Cuts at: 1246 1246
Size: 10487
SfoI GGC'GCC
Cuts at: 5604 7919 8167 5604
Size: 2315 248 7924
Fragments arranged by size:
7924 2315 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10487
SmaI CCC'GGG
Cuts at: 5560 7509 5560
Size: 1949 8538
SmlI C'TyrA_G
Cuts at: 1018 2584 3895 7342 7642 8557 9049 9311 1018
Size: 1566 1311 3447 300 915 492 262 2194
Fragments arranged by size:
3447 2194 1566 1311 915 492 300 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10487
SphI G_CATG'C
Cuts at: 936 7540 8040 936
Size: 6604 500 3383
Fragments arranged by size:
6604 3383 500
SrfI GCCC'GGGC
Cuts at: 5560 5560
Size: 10487
StuI AGG'CCT
Cuts at: 2180 6264 7222 7358 2180
Size: 4084 958 136 5309
Fragments arranged by size:
5309 4084 958 136
StyI C'CwwG_G
Cuts at: 107 1931 1987 3983 4211 4688 4823 5201 7460
Size: 1824 56 1996 228 477 135 378 2259
Cuts at: 7460 7621 7975 107
Size: 161 354 2619
Fragments arranged by size:
2619 2259 1996 1824 477 378 354 228
161 135 56
SwaI ATTT'AAAT
Cuts at: 5685 5685
Size: 10487
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 333 2350 3168 3445 4115 4669 5643 6037 6198
Size: 2017 818 277 670 554 974 394 161
Cuts at: 6198 6284 6708 6935 8070 8203 8662 8871 333
Size: 86 424 227 1135 133 459 209 1949
Fragments arranged by size:
2017 1949 1135 974 818 670 554 459
424 394 277 227 209 161 133 86
TatI w'GTAC_w
Cuts at: 1034 1545 2576 2828 2936 10002 1034
Size: 511 1031 252 108 7066 1519
Fragments arranged by size:
7066 1519 1031 511 252 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1183 1215 2203 2235 3050 3082 3793 3825 4968
Size: 32 988 32 815 32 711 32 1143
Cuts at: 4968 5000 5393 5425 5453 5485 7064 7096 10004
Size: 32 393 32 28 32 1579 32 2908
Cuts at: 10004 10036 1183
Size: 32 1634
Fragments arranged by size:
2908 1634 1579 1143 988 815 711 393
32 32 32 32 32 32 32 32
32 28
Tth111I GACn'n_nGTC
Cuts at: 7120 7336 8284 7120
Size: 216 948 9323
Fragments arranged by size:
9323 948 216
XbaI T'CTAG_A
Cuts at: 7518 7518
Size: 10487
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 603 1307 1725 2253 2857 4830 4908 5583 6042
Size: 704 418 528 604 1973 78 675 459
Cuts at: 6042 7356 7982 603
Size: 1314 626 3108
Fragments arranged by size:
3108 1973 1314 704 675 626 604 528
459 418 78
XmaI C'CCGG_G
Cuts at: 5558 7507 5558
Size: 1949 8538
ZraI GAC'GTC
Cuts at: 3933 3933
Size: 10487
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
FspAI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BmtI BsiWI BsrGI BssHII BstZ17I
FseI HpaI MfeI NdeI NheI NotI PacI PsiI
PspXI SanDI SgrAI SnaBI SspI XhoI XmnI