Restriction map for
pF1KB9001
(Circular) (Six-base) MAPSORT of: pF1KB9001.seq Check: 7863 from: 1 to: 4391
pF1KB9001 4391 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1429 1429
Size: 4391
Acc65I G'GTAC_C
Cuts at: 1407 1407
Size: 4391
AclI AA'CG_TT
Cuts at: 4231 4231
Size: 4391
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1226 2216 2648 3001 3663 1226
Size: 990 432 353 662 1954
Fragments arranged by size:
1954 990 662 432 353
AfeI AGC'GCT
Cuts at: 181 181
Size: 4391
AflIII A'CryG_T
Cuts at: 670 1743 3543 3824 670
Size: 1073 1800 281 1237
Fragments arranged by size:
1800 1237 1073 281
AhdI GACnn_n'nnGTC
Cuts at: 999 999
Size: 4391
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 746 778 825 857 746
Size: 32 47 32 4280
Fragments arranged by size:
4280 47 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1326 1696 3134 1326
Size: 370 1438 2583
Fragments arranged by size:
2583 1438 370
ApaLI G'TGCA_C
Cuts at: 3229 3229
Size: 4391
ApoI r'AATT_y
Cuts at: 847 1191 1395 3744 4000 847
Size: 344 204 2349 256 1238
Fragments arranged by size:
2349 1238 344 256 204
AseI AT'TA_AT
Cuts at: 20 20
Size: 4391
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4391
AvaI C'yCGr_G
Cuts at: 1411 1411
Size: 4391
BamHI G'GATC_C
Cuts at: 1416 1416
Size: 4391
BanI G'GyrC_C
Cuts at: 1101 1407 1821 2069 2104 1101
Size: 306 414 248 35 3388
Fragments arranged by size:
3388 414 306 248 35
BanII G_rGCy'C
Cuts at: 216 687 1405 2435 216
Size: 471 718 1030 2172
Fragments arranged by size:
2172 1030 718 471
BbeI G_GCGC'C
Cuts at: 1105 1825 2073 1105
Size: 720 248 3423
Fragments arranged by size:
3423 720 248
BbsI GAAGACnn'nnnn_
Cuts at: 372 372
Size: 4391
BciVI GTATCCnnnnn_n'
Cuts at: 2289 3345 4391 2289
Size: 1056 1046 2289
Fragments arranged by size:
2289 1056 1046
BclI T'GATC_A
Cuts at: 1911 1911
Size: 4391
BfrBI ATG'CAT
Cuts at: 746 746
Size: 4391
BglII A'GATC_T
Cuts at: 1906 3943 1906
Size: 2037 2354
BlpI GC'TnA_GC
Cuts at: 277 1503 277
Size: 1226 3165
Bme1580I G_kGCm'C
Cuts at: 2016 2109 3233 2016
Size: 93 1124 3174
Fragments arranged by size:
3174 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1603 1684 2019 4163 1603
Size: 81 335 2144 1831
Fragments arranged by size:
2144 1831 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1292 2758 1292
Size: 1466 2925
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 421
Size: 4391
Bpu10I CC'TnA_GC
Cuts at: 215 215
Size: 4391
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 1567 2446 3160 3458 160
Size: 1407 879 714 298 1093
Fragments arranged by size:
1407 1093 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4095 4095
Size: 4391
BsaAI yAC'GTr
Cuts at: 1744 1744
Size: 4391
BsaBI GATnn'nnATC
Cuts at: 6 1905 3942 6
Size: 1899 2037 455
Fragments arranged by size:
2037 1899 455
BsaHI Gr'CG_yC
Cuts at: 1049 1102 1822 2070 2772 4268 1049
Size: 53 720 248 702 1496 1172
Fragments arranged by size:
1496 1172 720 702 248 53
BsaWI w'CCGG_w
Cuts at: 1580 2101 2779 3190 3337 3611 1580
Size: 521 678 411 147 274 2360
Fragments arranged by size:
2360 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 746 776 1288 1318 746
Size: 30 512 30 3819
Fragments arranged by size:
3819 512 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 418 1293 418
Size: 875 3516
BseYI C'CCAG_C
Cuts at: 948 1023 1086 1816 2574 3239 3647 948
Size: 75 63 730 758 665 408 1692
Fragments arranged by size:
1692 758 730 665 408 75 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1268 1268
Size: 4391
BsiEI CG_ry'CG
Cuts at: 104 1933 1979 3209 104
Size: 1829 46 1230 1286
Fragments arranged by size:
1829 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 965 1405 2183 2373 3233 965
Size: 440 778 190 860 2123
Fragments arranged by size:
2123 860 778 440 190
Bsp1286I G_dGCh'C
Cuts at: 216 687 965 1405 2016 2109 2183 2373 2435
Size: 471 278 440 611 93 74 190 62
Cuts at: 2435 3233 216
Size: 798 1374
Fragments arranged by size:
1374 798 611 471 440 278 190 93
74 62
BspHI T'CATG_A
Cuts at: 335 647 851 2823 335
Size: 312 204 1972 1903
Fragments arranged by size:
1972 1903 312 204
BspMI ACCTGCnnnn'nnnn_
Cuts at: 506 1441 1655 1957 2338 506
Size: 935 214 302 381 2559
Fragments arranged by size:
2559 935 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2683 2737 4221 4386 50
Size: 2633 54 1484 165 55
Fragments arranged by size:
2633 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2303 2303
Size: 4391
BsrFI r'CCGG_y
Cuts at: 2389 2570 2389
Size: 181 4210
BssSI C'ACGA_G
Cuts at: 2662 3370 2662
Size: 708 3683
BstAPI GCAn_nnn'nTGC
Cuts at: 1768 1768
Size: 4391
BstBI TT'CG_AA
Cuts at: 2752 2752
Size: 4391
BstXI CCAn_nnnn'nTGG
Cuts at: 541 541
Size: 4391
BstYI r'GATC_y
Cuts at: 1 1416 1883 1906 2241 2487 2793 2805 2891
Size: 1415 467 23 335 246 306 12 86
Cuts at: 2891 3943 1
Size: 1052 449
Fragments arranged by size:
1415 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 689 689
Size: 4391
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2341 2522 2685 2341
Size: 181 163 4047
Fragments arranged by size:
4047 181 163
BtsI GCAGTG_nn'
Cuts at: 1259 1269 1634 1259
Size: 10 365 4016
Fragments arranged by size:
4016 365 10
DraI TTT'AAA
Cuts at: 563 1390 563
Size: 827 3564
DrdI GACnn_nn'nnGTC
Cuts at: 2097 3441 2097
Size: 1344 3047
EaeI y'GGCC_r
Cuts at: 263 623 1976 2150 2541 2568 3913 263
Size: 360 1353 174 391 27 1345 741
Fragments arranged by size:
1353 1345 741 391 360 174 27
EagI C'GGCC_G
Cuts at: 1976 1976
Size: 4391
EarI CTCTTCn'nnn_
Cuts at: 934 949 2414 2624 934
Size: 15 1465 210 2701
Fragments arranged by size:
2701 1465 210 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2599 3341 3487 4201 2599
Size: 742 146 714 2789
Fragments arranged by size:
2789 742 714 146
EcoICRI GAG'CTC
Cuts at: 1403 1403
Size: 4391
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 1226 2216 2648 3001 3663 421
Size: 805 990 432 353 662 1149
Fragments arranged by size:
1149 990 805 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1366 1530 1366
Size: 164 4227
EcoRI G'AATT_C
Cuts at: 847 1191 1395 847
Size: 344 204 3843
Fragments arranged by size:
3843 344 204
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 4391
FspI TGC'GCA
Cuts at: 2172 2172
Size: 4391
HaeII r_GCGC'y
Cuts at: 183 1105 1825 2073 3303 4074 183
Size: 922 720 248 1230 771 500
Fragments arranged by size:
1230 922 771 720 500 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 475 507 718 750 985 1017 1288 1320 2233
Size: 32 211 32 235 32 271 32 913
Cuts at: 2233 2263 2265 2295 475
Size: 30 2 30 2571
Fragments arranged by size:
2571 913 271 235 211 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 126 1430 126
Size: 1304 3087
HindIII A'AGCT_T
Cuts at: 642 642
Size: 4391
KasI G'GCGC_C
Cuts at: 1101 1821 2069 1101
Size: 720 248 3423
Fragments arranged by size:
3423 720 248
KpnI G_GTAC'C
Cuts at: 1411 1411
Size: 4391
MfeI C'AATT_G
Cuts at: 1151 1151
Size: 4391
MluI A'CGCG_T
Cuts at: 3824 3824
Size: 4391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1112 3150 3334 1112
Size: 2038 184 2169
Fragments arranged by size:
2169 2038 184
MscI TGG'CCA
Cuts at: 265 625 2152 265
Size: 360 1527 2504
Fragments arranged by size:
2504 1527 360
MslI CAynn'nnrTG
Cuts at: 2380 2507 2380
Size: 127 4264
MspA1I CmG'CkG
Cuts at: 952 1502 1816 2176 2960 3205 952
Size: 550 314 360 784 245 2138
Fragments arranged by size:
2138 784 550 360 314 245
NaeI GCC'GGC
Cuts at: 2572 2572
Size: 4391
NarI GG'CG_CC
Cuts at: 1102 1822 2070 1102
Size: 720 248 3423
Fragments arranged by size:
3423 720 248
NdeI CA'TA_TG
Cuts at: 660 660
Size: 4391
NgoMIV G'CCGG_C
Cuts at: 2570 2570
Size: 4391
NsiI A_TGCA'T
Cuts at: 748 748
Size: 4391
NspI r_CATG'y
Cuts at: 307 1444 1944 3547 307
Size: 1137 500 1603 1151
Fragments arranged by size:
1603 1151 1137 500
PasI CC'CwG_GG
Cuts at: 1371 1371
Size: 4391
PciI A'CATG_T
Cuts at: 3543 3543
Size: 4391
PflMI CCAn_nnn'nTGG
Cuts at: 1326 1326
Size: 4391
PfoI T'CCnGG_A
Cuts at: 608 783 608
Size: 175 4216
PmeI GTTT'AAAC
Cuts at: 1390 1390
Size: 4391
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 746 778 2837 2869 746
Size: 32 2059 32 2268
Fragments arranged by size:
2268 2059 32 32
PpuMI rG'GwC_Cy
Cuts at: 1366 1366
Size: 4391
PstI C_TGCA'G
Cuts at: 1438 2123 1438
Size: 685 3706
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4391
PvuII CAG'CTG
Cuts at: 1816 2176 1816
Size: 360 4031
RsrII CG'GwC_CG
Cuts at: 2586 2586
Size: 4391
SacI G_AGCT'C
Cuts at: 1405 1405
Size: 4391
SalI G'TCGA_C
Cuts at: 1428 1428
Size: 4391
SapI GCTCTTCn'nnn_
Cuts at: 2414 2624 2414
Size: 210 4181
SbfI CC_TGCA'GG
Cuts at: 1438 1438
Size: 4391
ScaI AGT'ACT
Cuts at: 3908 3908
Size: 4391
SfcI C'TryA_G
Cuts at: 33 138 1434 2119 3087 3278 33
Size: 105 1296 685 968 191 1146
Fragments arranged by size:
1296 1146 968 685 191 105
SfoI GGC'GCC
Cuts at: 1103 1823 2071 1103
Size: 720 248 3423
Fragments arranged by size:
3423 720 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4391
SmaI CCC'GGG
Cuts at: 1413 1413
Size: 4391
SmlI C'TyrA_G
Cuts at: 175 1546 2461 3175 3437 175
Size: 1371 915 714 262 1129
Fragments arranged by size:
1371 1129 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4391
SphI G_CATG'C
Cuts at: 1444 1944 1444
Size: 500 3891
StyI C'CwwG_G
Cuts at: 1525 1879 1525
Size: 354 4037
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1974 2107 2566 2775 1974
Size: 133 459 209 3590
Fragments arranged by size:
3590 459 209 133
TatI w'GTAC_w
Cuts at: 3906 3906
Size: 4391
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3908 3940 3908
Size: 32 4359
Tth111I GACn'n_nGTC
Cuts at: 2188 2188
Size: 4391
XbaI T'CTAG_A
Cuts at: 1422 1422
Size: 4391
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 514 1886 514
Size: 1372 3019
XmaI C'CCGG_G
Cuts at: 1411 1411
Size: 4391
XmnI GAAnn'nnTTC
Cuts at: 761 761
Size: 4391
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AhdI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI BsiWI
BsmI BsmBI BspEI BsrGI BssHII BstEII BstZ17I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
NcoI NheI NotI NruI PacI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI