Restriction map for
pF1KB8999
(Circular) (Six-base) MAPSORT of: pF1KB8999.seq Check: 4508 from: 1 to: 4184
pF1KB8999 4184 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1222 1222
Size: 4184
Acc65I G'GTAC_C
Cuts at: 1200 1200
Size: 4184
AclI AA'CG_TT
Cuts at: 4024 4024
Size: 4184
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2009 2441 3188 3456 2009
Size: 432 747 268 2737
Fragments arranged by size:
2737 747 432 268
AflIII A'CryG_T
Cuts at: 926 1176 1536 2640 3617 926
Size: 250 360 1104 977 1493
Fragments arranged by size:
1493 1104 977 360 250
AgeI A'CCGG_T
Cuts at: 2572 2572
Size: 4184
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 115 147 115
Size: 32 4152
AlwNI CAG_nnn'CTG
Cuts at: 494 1489 3056 494
Size: 995 1567 1622
Fragments arranged by size:
1622 1567 995
ApaLI G'TGCA_C
Cuts at: 403 2954 403
Size: 2551 1633
ApoI r'AATT_y
Cuts at: 1188 3537 3793 1188
Size: 2349 256 1579
Fragments arranged by size:
2349 1579 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4184
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
AvaI C'yCGr_G
Cuts at: 1204 1204
Size: 4184
BamHI G'GATC_C
Cuts at: 1001 1209 1001
Size: 208 3976
BanI G'GyrC_C
Cuts at: 419 727 1200 1614 1862 1897 419
Size: 308 473 414 248 35 2706
Fragments arranged by size:
2706 473 414 308 248 35
BanII G_rGCy'C
Cuts at: 805 1025 1198 2228 805
Size: 220 173 1030 2761
Fragments arranged by size:
2761 1030 220 173
BbeI G_GCGC'C
Cuts at: 423 731 1618 1866 423
Size: 308 887 248 2741
Fragments arranged by size:
2741 887 308 248
BbsI GAAGACnn'nnnn_
Cuts at: 213 817 213
Size: 604 3580
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 418 452 418
Size: 34 4150
BciVI GTATCCnnnnn_n'
Cuts at: 2082 2843 4184 2082
Size: 761 1341 2082
Fragments arranged by size:
2082 1341 761
BclI T'GATC_A
Cuts at: 1704 1704
Size: 4184
BglII A'GATC_T
Cuts at: 773 1699 3736 773
Size: 926 2037 1221
Fragments arranged by size:
2037 1221 926
BlpI GC'TnA_GC
Cuts at: 394 1296 394
Size: 902 3282
Bme1580I G_kGCm'C
Cuts at: 332 407 609 1809 1902 2958 332
Size: 75 202 1200 93 1056 1558
Fragments arranged by size:
1558 1200 1056 202 93 75
BmgBI CAC'GTC
Cuts at: 471 471
Size: 4184
BmrI ACTGGGnnnn_n'
Cuts at: 1396 1477 1812 3956 1396
Size: 81 335 2144 1624
Fragments arranged by size:
2144 1624 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 678 2551 678
Size: 1873 2311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 789 789
Size: 4184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1360 2239 2731 3029 1360
Size: 879 492 298 2515
Fragments arranged by size:
2515 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 157 3888 157
Size: 3731 453
BsaAI yAC'GTr
Cuts at: 929 1537 929
Size: 608 3576
BsaBI GATnn'nnATC
Cuts at: 6 1698 3735 6
Size: 1692 2037 455
Fragments arranged by size:
2037 1692 455
BsaHI Gr'CG_yC
Cuts at: 420 728 1615 1863 2565 4061 420
Size: 308 887 248 702 1496 543
Fragments arranged by size:
1496 887 702 543 308 248
BsaWI w'CCGG_w
Cuts at: 1373 1894 2572 2846 2993 3404 1373
Size: 521 678 274 147 411 2153
Fragments arranged by size:
2153 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 115 145 1081 1111 115
Size: 30 936 30 3188
Fragments arranged by size:
3188 936 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 691 691
Size: 4184
BseYI C'CCAG_C
Cuts at: 199 246 647 1609 2367 2944 3440 199
Size: 47 401 962 758 577 496 943
Fragments arranged by size:
962 943 758 577 496 401 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 465 465
Size: 4184
BsiEI CG_ry'CG
Cuts at: 104 1726 1772 2980 104
Size: 1622 46 1208 1308
Fragments arranged by size:
1622 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 407 758 995 1025 1198 1976 2166 2958 407
Size: 351 237 30 173 778 190 792 1633
Fragments arranged by size:
1633 792 778 351 237 190 173 30
BsmBI CGTCTCn'nnnn_
Cuts at: 363 582 649 828 363
Size: 219 67 179 3719
Fragments arranged by size:
3719 219 179 67
Bsp1286I G_dGCh'C
Cuts at: 332 407 609 758 805 995 1025 1198 1809
Size: 75 202 149 47 190 30 173 611
Cuts at: 1809 1902 1976 2166 2228 2958 332
Size: 93 74 190 62 730 1558
Fragments arranged by size:
1558 730 611 202 190 190 173 149
93 75 74 62 47 30
BspEI T'CCGG_A
Cuts at: 3404 3404
Size: 4184
BspHI T'CATG_A
Cuts at: 587 3360 587
Size: 2773 1411
BspMI ACCTGCnnnn'nnnn_
Cuts at: 200 290 746 1234 1448 1750 2131 200
Size: 90 456 488 214 302 381 2253
Fragments arranged by size:
2253 488 456 381 302 214 90
BsrBI CCG'CTC
Cuts at: 50 744 2476 2530 4014 4179 50
Size: 694 1732 54 1484 165 55
Fragments arranged by size:
1732 1484 694 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2096 2096
Size: 4184
BsrFI r'CCGG_y
Cuts at: 2182 2363 2572 2182
Size: 181 209 3794
Fragments arranged by size:
3794 209 181
BssSI C'ACGA_G
Cuts at: 406 2455 2813 406
Size: 2049 358 1777
Fragments arranged by size:
2049 1777 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1561 1561
Size: 4184
BstBI TT'CG_AA
Cuts at: 2545 2545
Size: 4184
BstXI CCAn_nnnn'nTGG
Cuts at: 516 516
Size: 4184
BstYI r'GATC_y
Cuts at: 1 773 1001 1209 1676 1699 2034 2280 3292
Size: 772 228 208 467 23 335 246 1012
Cuts at: 3292 3378 3390 3736 1
Size: 86 12 346 449
Fragments arranged by size:
1012 772 467 449 346 335 246 228
208 86 23 12
BtgI C'CryG_G
Cuts at: 107 350 107
Size: 243 3941
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2134 2315 2478 2134
Size: 181 163 3840
Fragments arranged by size:
3840 181 163
BtsI GCAGTG_nn'
Cuts at: 1427 1427
Size: 4184
DraI TTT'AAA
Cuts at: 1183 1183
Size: 4184
DraIII CAC_nnn'GTG
Cuts at: 327 327
Size: 4184
DrdI GACnn_nn'nnGTC
Cuts at: 1890 2748 1890
Size: 858 3326
EaeI y'GGCC_r
Cuts at: 353 374 485 506 1769 1943 2334 2361 3706
Size: 21 111 21 1263 174 391 27 1345
Cuts at: 3706 353
Size: 831
Fragments arranged by size:
1345 1263 831 391 174 111 27 21
21
EagI C'GGCC_G
Cuts at: 1769 1769
Size: 4184
EarI CTCTTCn'nnn_
Cuts at: 763 2207 2417 763
Size: 1444 210 2530
Fragments arranged by size:
2530 1444 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 880 2392 2702 2848 3994 880
Size: 1512 310 146 1146 1070
Fragments arranged by size:
1512 1146 1070 310 146
EcoICRI GAG'CTC
Cuts at: 1023 1196 1023
Size: 173 4011
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 789 2009 2441 3188 3456 789
Size: 1220 432 747 268 1517
Fragments arranged by size:
1517 1220 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1323 1323
Size: 4184
EcoRI G'AATT_C
Cuts at: 1188 1188
Size: 4184
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1179 2203 1179
Size: 1024 3160
FspI TGC'GCA
Cuts at: 1965 1965
Size: 4184
HaeII r_GCGC'y
Cuts at: 423 731 1151 1618 1866 2888 3867 423
Size: 308 420 467 248 1022 979 740
Fragments arranged by size:
1022 979 740 467 420 308 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2026 2056 2058 2088 2026
Size: 30 2 30 4122
Fragments arranged by size:
4122 30 30 2
HincII GTy'rAC
Cuts at: 1223 1223
Size: 4184
KasI G'GCGC_C
Cuts at: 419 727 1614 1862 419
Size: 308 887 248 2741
Fragments arranged by size:
2741 887 308 248
KpnI G_GTAC'C
Cuts at: 1204 1204
Size: 4184
MluI A'CGCG_T
Cuts at: 3617 3617
Size: 4184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 418 605 1118 2855 3039 418
Size: 187 513 1737 184 1563
Fragments arranged by size:
1737 1563 513 187 184
MscI TGG'CCA
Cuts at: 1945 1945
Size: 4184
MslI CAynn'nnrTG
Cuts at: 982 2173 2300 982
Size: 1191 127 2866
Fragments arranged by size:
2866 1191 127
MspA1I CmG'CkG
Cuts at: 393 425 616 636 951 1295 1609 1969 2982
Size: 32 191 20 315 344 314 360 1013
Cuts at: 2982 3227 393
Size: 245 1350
Fragments arranged by size:
1350 1013 360 344 315 314 245 191
32 20
NaeI GCC'GGC
Cuts at: 2365 2365
Size: 4184
NarI GG'CG_CC
Cuts at: 420 728 1615 1863 420
Size: 308 887 248 2741
Fragments arranged by size:
2741 887 308 248
NcoI C'CATG_G
Cuts at: 107 350 107
Size: 243 3941
NgoMIV G'CCGG_C
Cuts at: 2363 2363
Size: 4184
NspI r_CATG'y
Cuts at: 1237 1737 2644 1237
Size: 500 907 2777
Fragments arranged by size:
2777 907 500
PciI A'CATG_T
Cuts at: 2640 2640
Size: 4184
PflMI CCAn_nnn'nTGG
Cuts at: 339 494 339
Size: 155 4029
PfoI T'CCnGG_A
Cuts at: 805 805
Size: 4184
PmeI GTTT'AAAC
Cuts at: 1183 1183
Size: 4184
PmlI CAC'GTG
Cuts at: 929 929
Size: 4184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 115 147 3323 3355 115
Size: 32 3176 32 944
Fragments arranged by size:
3176 944 32 32
PstI C_TGCA'G
Cuts at: 1231 1916 1231
Size: 685 3499
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4184
PvuII CAG'CTG
Cuts at: 1609 1969 1609
Size: 360 3824
RsrII CG'GwC_CG
Cuts at: 888 2379 888
Size: 1491 2693
SacI G_AGCT'C
Cuts at: 1025 1198 1025
Size: 173 4011
SalI G'TCGA_C
Cuts at: 1221 1221
Size: 4184
SapI GCTCTTCn'nnn_
Cuts at: 763 2207 2417 763
Size: 1444 210 2530
Fragments arranged by size:
2530 1444 210
SbfI CC_TGCA'GG
Cuts at: 1231 1231
Size: 4184
ScaI AGT'ACT
Cuts at: 3701 3701
Size: 4184
SexAI A'CCwGG_T
Cuts at: 338 338
Size: 4184
SfcI C'TryA_G
Cuts at: 33 1227 1912 2905 3096 33
Size: 1194 685 993 191 1121
Fragments arranged by size:
1194 1121 993 685 191
SfoI GGC'GCC
Cuts at: 421 729 1616 1864 421
Size: 308 887 248 2741
Fragments arranged by size:
2741 887 308 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
SmaI CCC'GGG
Cuts at: 1206 1206
Size: 4184
SmlI C'TyrA_G
Cuts at: 1339 2254 2746 3008 1339
Size: 915 492 262 2515
Fragments arranged by size:
2515 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4184
SphI G_CATG'C
Cuts at: 1237 1737 1237
Size: 500 3684
StyI C'CwwG_G
Cuts at: 107 350 1318 1672 107
Size: 243 968 354 2619
Fragments arranged by size:
2619 968 354 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 207 843 1767 1900 2359 2568 207
Size: 636 924 133 459 209 1823
Fragments arranged by size:
1823 924 636 459 209 133
TatI w'GTAC_w
Cuts at: 764 3699 764
Size: 2935 1249
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 397 429 3701 3733 397
Size: 32 3272 32 848
Fragments arranged by size:
3272 848 32 32
Tth111I GACn'n_nGTC
Cuts at: 1981 1981
Size: 4184
XbaI T'CTAG_A
Cuts at: 1215 1215
Size: 4184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 795 1679 795
Size: 884 3300
XmaI C'CCGG_G
Cuts at: 1204 1204
Size: 4184
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmtI Bpu10I
BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI