Restriction map for
pF1KB8997
(Circular) (Six-base) MAPSORT of: pF1KB8997.seq Check: 1696 from: 1 to: 4337
pF1KB8997 4337 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 588 588
Size: 4337
AccI GT'mk_AC
Cuts at: 1375 1375
Size: 4337
Acc65I G'GTAC_C
Cuts at: 1353 1353
Size: 4337
AclI AA'CG_TT
Cuts at: 4177 4177
Size: 4337
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 1041 2162 2594 3341 3609 358
Size: 683 1121 432 747 268 1086
Fragments arranged by size:
1121 1086 747 683 432 268
AflIII A'CryG_T
Cuts at: 1689 2793 3770 1689
Size: 1104 977 2256
Fragments arranged by size:
2256 1104 977
AgeI A'CCGG_T
Cuts at: 2725 2725
Size: 4337
AlwNI CAG_nnn'CTG
Cuts at: 1185 1642 3209 1185
Size: 457 1567 2313
Fragments arranged by size:
2313 1567 457
ApaI G_GGCC'C
Cuts at: 613 906 613
Size: 293 4044
ApaLI G'TGCA_C
Cuts at: 3107 3107
Size: 4337
ApoI r'AATT_y
Cuts at: 1341 3690 3946 1341
Size: 2349 256 1732
Fragments arranged by size:
2349 1732 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4337
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4337
AvaI C'yCGr_G
Cuts at: 782 1357 782
Size: 575 3762
BamHI G'GATC_C
Cuts at: 1362 1362
Size: 4337
BanI G'GyrC_C
Cuts at: 678 686 1034 1325 1353 1767 2015 2050 678
Size: 8 348 291 28 414 248 35 2965
Fragments arranged by size:
2965 414 348 291 248 35 28 8
BanII G_rGCy'C
Cuts at: 266 613 763 906 1351 2381 266
Size: 347 150 143 445 1030 2222
Fragments arranged by size:
2222 1030 445 347 150 143
BbeI G_GCGC'C
Cuts at: 1038 1771 2019 1038
Size: 733 248 3356
Fragments arranged by size:
3356 733 248
BbvCI CC'TCA_GC
Cuts at: 538 538
Size: 4337
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 273 307 273
Size: 34 4303
BciVI GTATCCnnnnn_n'
Cuts at: 434 1219 2235 2996 4337 434
Size: 785 1016 761 1341 434
Fragments arranged by size:
1341 1016 785 761 434
BclI T'GATC_A
Cuts at: 1857 1857
Size: 4337
BglI GCCn_nnn'nGGC
Cuts at: 445 941 445
Size: 496 3841
BglII A'GATC_T
Cuts at: 1852 3889 1852
Size: 2037 2300
BlpI GC'TnA_GC
Cuts at: 1449 1449
Size: 4337
Bme1580I G_kGCm'C
Cuts at: 613 689 906 1232 1962 2055 3111 613
Size: 76 217 326 730 93 1056 1839
Fragments arranged by size:
1839 1056 730 326 217 93 76
BmrI ACTGGGnnnn_n'
Cuts at: 1549 1630 1965 4109 1549
Size: 81 335 2144 1777
Fragments arranged by size:
2144 1777 335 81
BmtI G_CTAG'C
Cuts at: 811 811
Size: 4337
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 582 2704 582
Size: 2122 2215
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 505
Size: 4337
Bpu10I CC'TnA_GC
Cuts at: 246 428 538 989 246
Size: 182 110 451 3594
Fragments arranged by size:
3594 451 182 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 869 1513 2392 2884 3182 439
Size: 430 644 879 492 298 1594
Fragments arranged by size:
1594 879 644 492 430 298
BsaI GGTCTCn'nnnn_
Cuts at: 4041 4041
Size: 4337
BsaAI yAC'GTr
Cuts at: 1690 1690
Size: 4337
BsaBI GATnn'nnATC
Cuts at: 6 1851 3888 6
Size: 1845 2037 455
Fragments arranged by size:
2037 1845 455
BsaHI Gr'CG_yC
Cuts at: 240 966 1035 1768 2016 2718 4214 240
Size: 726 69 733 248 702 1496 363
Fragments arranged by size:
1496 733 726 702 363 248 69
BsaWI w'CCGG_w
Cuts at: 345 1526 2047 2725 2999 3146 3557 345
Size: 1181 521 678 274 147 411 1125
Fragments arranged by size:
1181 1125 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 400 403 474 558 400
Size: 3 71 84 4179
Fragments arranged by size:
4179 84 71 3
BseYI C'CCAG_C
Cuts at: 666 905 1762 2520 3097 3593 666
Size: 239 857 758 577 496 1410
Fragments arranged by size:
1410 857 758 577 496 239
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1198 1198
Size: 4337
BsiEI CG_ry'CG
Cuts at: 104 877 1879 1925 3133 104
Size: 773 1002 46 1208 1308
Fragments arranged by size:
1308 1208 1002 773 46
BsiHKAI G_wGCw'C
Cuts at: 266 1351 2129 2319 3111 266
Size: 1085 778 190 792 1492
Fragments arranged by size:
1492 1085 792 778 190
BsmI GAATG_Cn'
Cuts at: 131 131
Size: 4337
BsmBI CGTCTCn'nnnn_
Cuts at: 187 187
Size: 4337
Bsp1286I G_dGCh'C
Cuts at: 266 613 689 763 906 1232 1351 1962 2055
Size: 347 76 74 143 326 119 611 93
Cuts at: 2055 2129 2319 2381 3111 266
Size: 74 190 62 730 1492
Fragments arranged by size:
1492 730 611 347 326 190 143 119
93 76 74 74 62
BspEI T'CCGG_A
Cuts at: 345 3557 345
Size: 3212 1125
BspHI T'CATG_A
Cuts at: 3513 3513
Size: 4337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 588 1387 1601 1903 2284 588
Size: 799 214 302 381 2641
Fragments arranged by size:
2641 799 381 302 214
BsrBI CCG'CTC
Cuts at: 50 245 2629 2683 4167 4332 50
Size: 195 2384 54 1484 165 55
Fragments arranged by size:
2384 1484 195 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2249 2249
Size: 4337
BsrFI r'CCGG_y
Cuts at: 368 1031 2335 2516 2725 368
Size: 663 1304 181 209 1980
Fragments arranged by size:
1980 1304 663 209 181
BssSI C'ACGA_G
Cuts at: 711 2608 2966 711
Size: 1897 358 2082
Fragments arranged by size:
2082 1897 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1714 1714
Size: 4337
BstBI TT'CG_AA
Cuts at: 2698 2698
Size: 4337
BstEII G'GTnAC_C
Cuts at: 118 924 118
Size: 806 3531
BstXI CCAn_nnnn'nTGG
Cuts at: 412 412
Size: 4337
BstYI r'GATC_y
Cuts at: 1 360 401 1362 1829 1852 2187 2433 3445
Size: 359 41 961 467 23 335 246 1012
Cuts at: 3445 3531 3543 3889 1
Size: 86 12 346 449
Fragments arranged by size:
1012 961 467 449 359 346 335 246
86 41 23 12
BtgI C'CryG_G
Cuts at: 1119 1119
Size: 4337
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1076 2287 2468 2631 1076
Size: 1211 181 163 2782
Fragments arranged by size:
2782 1211 181 163
BtsI GCAGTG_nn'
Cuts at: 1580 1580
Size: 4337
DraI TTT'AAA
Cuts at: 1336 1336
Size: 4337
DraIII CAC_nnn'GTG
Cuts at: 732 732
Size: 4337
DrdI GACnn_nn'nnGTC
Cuts at: 2043 2901 2043
Size: 858 3479
EaeI y'GGCC_r
Cuts at: 437 933 942 1922 2096 2487 2514 3859 437
Size: 496 9 980 174 391 27 1345 915
Fragments arranged by size:
1345 980 915 496 391 174 27 9
EagI C'GGCC_G
Cuts at: 1922 1922
Size: 4337
EarI CTCTTCn'nnn_
Cuts at: 389 592 2360 2570 389
Size: 203 1768 210 2156
Fragments arranged by size:
2156 1768 210 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2545 2855 3001 4147 2545
Size: 310 146 1146 2735
Fragments arranged by size:
2735 1146 310 146
EcoICRI GAG'CTC
Cuts at: 264 1349 264
Size: 1085 3252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 505 1041 2162 2594 3341 3609 358
Size: 147 536 1121 432 747 268 1086
Fragments arranged by size:
1121 1086 747 536 432 268 147
EcoO109I rG'GnC_Cy
Cuts at: 609 610 1476 609
Size: 1 866 3470
Fragments arranged by size:
3470 866 1
EcoRI G'AATT_C
Cuts at: 1341 1341
Size: 4337
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2356 2356
Size: 4337
FspI TGC'GCA
Cuts at: 2118 2118
Size: 4337
HaeII r_GCGC'y
Cuts at: 435 1038 1771 2019 3041 4020 435
Size: 603 733 248 1022 979 752
Fragments arranged by size:
1022 979 752 733 603 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2179 2209 2211 2241 2179
Size: 30 2 30 4275
Fragments arranged by size:
4275 30 30 2
HincII GTy'rAC
Cuts at: 1376 1376
Size: 4337
KasI G'GCGC_C
Cuts at: 1034 1767 2015 1034
Size: 733 248 3356
Fragments arranged by size:
3356 733 248
KpnI G_GTAC'C
Cuts at: 1357 1357
Size: 4337
MluI A'CGCG_T
Cuts at: 3770 3770
Size: 4337
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3008 3192 3008
Size: 184 4153
MscI TGG'CCA
Cuts at: 935 2098 935
Size: 1163 3174
MslI CAynn'nnrTG
Cuts at: 728 817 1252 2326 2453 728
Size: 89 435 1074 127 2612
Fragments arranged by size:
2612 1074 435 127 89
MspA1I CmG'CkG
Cuts at: 1005 1448 1762 2122 3135 3380 1005
Size: 443 314 360 1013 245 1962
Fragments arranged by size:
1962 1013 443 360 314 245
NaeI GCC'GGC
Cuts at: 1033 2518 1033
Size: 1485 2852
NarI GG'CG_CC
Cuts at: 1035 1768 2016 1035
Size: 733 248 3356
Fragments arranged by size:
3356 733 248
NcoI C'CATG_G
Cuts at: 1119 1119
Size: 4337
NgoMIV G'CCGG_C
Cuts at: 1031 2516 1031
Size: 1485 2852
NheI G'CTAG_C
Cuts at: 807 807
Size: 4337
NspI r_CATG'y
Cuts at: 357 862 1390 1890 2797 357
Size: 505 528 500 907 1897
Fragments arranged by size:
1897 907 528 505 500
PasI CC'CwG_GG
Cuts at: 496 496
Size: 4337
PciI A'CATG_T
Cuts at: 2793 2793
Size: 4337
PflMI CCAn_nnn'nTGG
Cuts at: 802 802
Size: 4337
PfoI T'CCnGG_A
Cuts at: 590 590
Size: 4337
PmeI GTTT'AAAC
Cuts at: 1336 1336
Size: 4337
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3476 3508 3476
Size: 32 4305
PshAI GACnn'nnGTC
Cuts at: 1186 1186
Size: 4337
PspOMI G'GGCC_C
Cuts at: 609 902 609
Size: 293 4044
PstI C_TGCA'G
Cuts at: 296 576 1384 2069 296
Size: 280 808 685 2564
Fragments arranged by size:
2564 808 685 280
PvuI CG_AT'CG
Cuts at: 104 877 104
Size: 773 3564
PvuII CAG'CTG
Cuts at: 1762 2122 1762
Size: 360 3977
RsrII CG'GwC_CG
Cuts at: 2532 2532
Size: 4337
SacI G_AGCT'C
Cuts at: 266 1351 266
Size: 1085 3252
SalI G'TCGA_C
Cuts at: 1374 1374
Size: 4337
SapI GCTCTTCn'nnn_
Cuts at: 389 592 2360 2570 389
Size: 203 1768 210 2156
Fragments arranged by size:
2156 1768 210 203
SbfI CC_TGCA'GG
Cuts at: 1384 1384
Size: 4337
ScaI AGT'ACT
Cuts at: 3854 3854
Size: 4337
SfcI C'TryA_G
Cuts at: 33 292 572 1380 2065 3058 3249 33
Size: 259 280 808 685 993 191 1121
Fragments arranged by size:
1121 993 808 685 280 259 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 445 941 445
Size: 496 3841
SfoI GGC'GCC
Cuts at: 1036 1769 2017 1036
Size: 733 248 3356
Fragments arranged by size:
3356 733 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4337
SmaI CCC'GGG
Cuts at: 784 1359 784
Size: 575 3762
SmlI C'TyrA_G
Cuts at: 418 884 1492 2407 2899 3161 418
Size: 466 608 915 492 262 1594
Fragments arranged by size:
1594 915 608 492 466 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4337
SphI G_CATG'C
Cuts at: 1390 1890 1390
Size: 500 3837
StuI AGG'CCT
Cuts at: 1108 1108
Size: 4337
StyI C'CwwG_G
Cuts at: 1119 1471 1825 1119
Size: 352 354 3631
Fragments arranged by size:
3631 354 352
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1313 1920 2053 2512 2721 1313
Size: 607 133 459 209 2929
Fragments arranged by size:
2929 607 459 209 133
TatI w'GTAC_w
Cuts at: 3852 3852
Size: 4337
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3854 3886 3854
Size: 32 4305
Tth111I GACn'n_nGTC
Cuts at: 2134 2134
Size: 4337
XbaI T'CTAG_A
Cuts at: 1368 1368
Size: 4337
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1260 1832 1260
Size: 572 3765
XmaI C'CCGG_G
Cuts at: 782 1357 782
Size: 575 3762
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BfrBI BmgBI BsaXI BsiWI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NotI NruI NsiI
PacI PmlI PpuMI PsiI PspXI PsrI SacII SanDI
SexAI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI