Restriction map for
pF1KB8994
(Circular) (Six-base) MAPSORT of: pF1KB8994.seq Check: 2755 from: 1 to: 4100
pF1KB8994 4100 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 261 261
Size: 4100
AccI GT'mk_AC
Cuts at: 368 1079 1138 368
Size: 711 59 3330
Fragments arranged by size:
3330 711 59
Acc65I G'GTAC_C
Cuts at: 1116 1116
Size: 4100
AclI AA'CG_TT
Cuts at: 1094 3940 1094
Size: 2846 1254
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1925 2357 3104 3372 1925
Size: 432 747 268 2653
Fragments arranged by size:
2653 747 432 268
AfeI AGC'GCT
Cuts at: 433 961 433
Size: 528 3572
AflIII A'CryG_T
Cuts at: 1452 2556 3533 1452
Size: 1104 977 2019
Fragments arranged by size:
2019 1104 977
AgeI A'CCGG_T
Cuts at: 2488 2488
Size: 4100
AlwNI CAG_nnn'CTG
Cuts at: 1008 1405 2972 1008
Size: 397 1567 2136
Fragments arranged by size:
2136 1567 397
ApaI G_GGCC'C
Cuts at: 756 821 756
Size: 65 4035
ApaLI G'TGCA_C
Cuts at: 421 2870 421
Size: 2449 1651
ApoI r'AATT_y
Cuts at: 1104 3453 3709 1104
Size: 2349 256 1495
Fragments arranged by size:
2349 1495 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4100
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4100
AvaI C'yCGr_G
Cuts at: 749 1025 1120 749
Size: 276 95 3729
Fragments arranged by size:
3729 276 95
AvrII C'CTAG_G
Cuts at: 906 906
Size: 4100
BamHI G'GATC_C
Cuts at: 1125 1125
Size: 4100
BanI G'GyrC_C
Cuts at: 132 559 995 1116 1530 1778 1813 132
Size: 427 436 121 414 248 35 2419
Fragments arranged by size:
2419 436 427 414 248 121 35
BanII G_rGCy'C
Cuts at: 167 623 737 756 821 1114 2144 167
Size: 456 114 19 65 293 1030 2123
Fragments arranged by size:
2123 1030 456 293 114 65 19
BbeI G_GCGC'C
Cuts at: 1534 1782 1534
Size: 248 3852
BciVI GTATCCnnnnn_n'
Cuts at: 1998 2759 4100 1998
Size: 761 1341 1998
Fragments arranged by size:
1998 1341 761
BclI T'GATC_A
Cuts at: 1620 1620
Size: 4100
BglI GCCn_nnn'nGGC
Cuts at: 876 876
Size: 4100
BglII A'GATC_T
Cuts at: 1615 3652 1615
Size: 2037 2063
BlpI GC'TnA_GC
Cuts at: 1212 1212
Size: 4100
Bme1580I G_kGCm'C
Cuts at: 137 425 756 780 821 935 1725 1818 2874
Size: 288 331 24 41 114 790 93 1056
Cuts at: 2874 137
Size: 1363
Fragments arranged by size:
1363 1056 790 331 288 114 93 41
24
BmrI ACTGGGnnnn_n'
Cuts at: 883 1312 1393 1728 3872 883
Size: 429 81 335 2144 1111
Fragments arranged by size:
2144 1111 429 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 4100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 730 783 806 996 442
Size: 288 53 23 190 3546
Fragments arranged by size:
3546 288 190 53 23
Bpu10I CC'TnA_GC
Cuts at: 271 271
Size: 4100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1276 2155 2647 2945 1276
Size: 879 492 298 2431
Fragments arranged by size:
2431 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3804 3804
Size: 4100
BsaAI yAC'GTr
Cuts at: 825 1453 825
Size: 628 3472
BsaBI GATnn'nnATC
Cuts at: 6 1614 3651 6
Size: 1608 2037 455
Fragments arranged by size:
2037 1608 455
BsaHI Gr'CG_yC
Cuts at: 112 1531 1779 2481 3977 112
Size: 1419 248 702 1496 235
Fragments arranged by size:
1496 1419 702 248 235
BsaWI w'CCGG_w
Cuts at: 1289 1810 2488 2762 2909 3320 1289
Size: 521 678 274 147 411 2069
Fragments arranged by size:
2069 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 556 586 556
Size: 30 4070
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 352 481 829 889 352
Size: 129 348 60 3563
Fragments arranged by size:
3563 348 129 60
BseYI C'CCAG_C
Cuts at: 136 286 588 812 948 1042 1525 2283 2860
Size: 150 302 224 136 94 483 758 577
Cuts at: 2860 3356 136
Size: 496 880
Fragments arranged by size:
880 758 577 496 483 302 224 150
136 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 941 941
Size: 4100
BsiEI CG_ry'CG
Cuts at: 104 1642 1688 2896 104
Size: 1538 46 1208 1308
Fragments arranged by size:
1538 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 425 1114 1892 2082 2874 425
Size: 689 778 190 792 1651
Fragments arranged by size:
1651 792 778 689 190
BsmI GAATG_Cn'
Cuts at: 473 473
Size: 4100
Bsp1286I G_dGCh'C
Cuts at: 137 167 425 623 737 756 780 821 935
Size: 30 258 198 114 19 24 41 114
Cuts at: 935 1114 1725 1818 1892 2082 2144 2874 137
Size: 179 611 93 74 190 62 730 1363
Fragments arranged by size:
1363 730 611 258 198 190 179 114
114 93 74 62 41 30 24 19
BspEI T'CCGG_A
Cuts at: 3320 3320
Size: 4100
BspHI T'CATG_A
Cuts at: 3276 3276
Size: 4100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 212 261 1150 1364 1666 2047 212
Size: 49 889 214 302 381 2265
Fragments arranged by size:
2265 889 381 302 214 49
BsrBI CCG'CTC
Cuts at: 50 532 714 2392 2446 3930 4095 50
Size: 482 182 1678 54 1484 165 55
Fragments arranged by size:
1678 1484 482 182 165 55 54
BsrDI GCAATG_nn'
Cuts at: 307 313 1046 2012 307
Size: 6 733 966 2395
Fragments arranged by size:
2395 966 733 6
BsrFI r'CCGG_y
Cuts at: 704 2098 2279 2488 704
Size: 1394 181 209 2316
Fragments arranged by size:
2316 1394 209 181
BssSI C'ACGA_G
Cuts at: 2371 2729 2371
Size: 358 3742
BstAPI GCAn_nnn'nTGC
Cuts at: 1477 1477
Size: 4100
BstBI TT'CG_AA
Cuts at: 2461 2461
Size: 4100
BstXI CCAn_nnnn'nTGG
Cuts at: 326 466 948 326
Size: 140 482 3478
Fragments arranged by size:
3478 482 140
BstYI r'GATC_y
Cuts at: 1 1125 1592 1615 1950 2196 3208 3294 3306
Size: 1124 467 23 335 246 1012 86 12
Cuts at: 3306 3652 1
Size: 346 449
Fragments arranged by size:
1124 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 448 448
Size: 4100
BtgI C'CryG_G
Cuts at: 635 941 635
Size: 306 3794
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 207 743 2050 2231 2394 207
Size: 536 1307 181 163 1913
Fragments arranged by size:
1913 1307 536 181 163
BtsI GCAGTG_nn'
Cuts at: 674 725 832 935 1080 1343 674
Size: 51 107 103 145 263 3431
Fragments arranged by size:
3431 263 145 107 103 51
DraI TTT'AAA
Cuts at: 1099 1099
Size: 4100
DrdI GACnn_nn'nnGTC
Cuts at: 891 1806 2664 891
Size: 915 858 2327
Fragments arranged by size:
2327 915 858
EaeI y'GGCC_r
Cuts at: 671 827 1685 1859 2250 2277 3622 671
Size: 156 858 174 391 27 1345 1149
Fragments arranged by size:
1345 1149 858 391 174 156 27
EagI C'GGCC_G
Cuts at: 1685 1685
Size: 4100
EarI CTCTTCn'nnn_
Cuts at: 805 1011 2123 2333 805
Size: 206 1112 210 2572
Fragments arranged by size:
2572 1112 210 206
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 729 2308 2618 2764 3910 729
Size: 1579 310 146 1146 919
Fragments arranged by size:
1579 1146 919 310 146
EcoICRI GAG'CTC
Cuts at: 1112 1112
Size: 4100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 730 783 806 996 1925 2357 3104 3372
Size: 288 53 23 190 929 432 747 268
Cuts at: 3372 442
Size: 1170
Fragments arranged by size:
1170 929 747 432 288 268 190 53
23
EcoO109I rG'GnC_Cy
Cuts at: 753 817 818 877 1239 753
Size: 64 1 59 362 3614
Fragments arranged by size:
3614 362 64 59 1
EcoRI G'AATT_C
Cuts at: 1104 1104
Size: 4100
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2119 2119
Size: 4100
FspI TGC'GCA
Cuts at: 244 1881 244
Size: 1637 2463
HaeII r_GCGC'y
Cuts at: 435 867 963 1534 1782 2804 3783 435
Size: 432 96 571 248 1022 979 752
Fragments arranged by size:
1022 979 752 571 432 248 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 721 753 1942 1972 1974 2004 721
Size: 32 1189 30 2 30 2817
Fragments arranged by size:
2817 1189 32 30 30 2
HincII GTy'rAC
Cuts at: 1080 1139 1080
Size: 59 4041
KasI G'GCGC_C
Cuts at: 1530 1778 1530
Size: 248 3852
KpnI G_GTAC'C
Cuts at: 1120 1120
Size: 4100
MluI A'CGCG_T
Cuts at: 3533 3533
Size: 4100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2771 2955 2771
Size: 184 3916
MscI TGG'CCA
Cuts at: 673 829 1861 673
Size: 156 1032 2912
Fragments arranged by size:
2912 1032 156
MslI CAynn'nnrTG
Cuts at: 2089 2216 2089
Size: 127 3973
MspA1I CmG'CkG
Cuts at: 690 925 1211 1525 1885 2898 3143 690
Size: 235 286 314 360 1013 245 1647
Fragments arranged by size:
1647 1013 360 314 286 245 235
NaeI GCC'GGC
Cuts at: 706 2281 706
Size: 1575 2525
NarI GG'CG_CC
Cuts at: 1531 1779 1531
Size: 248 3852
NcoI C'CATG_G
Cuts at: 941 941
Size: 4100
NgoMIV G'CCGG_C
Cuts at: 704 2279 704
Size: 1575 2525
NspI r_CATG'y
Cuts at: 1153 1653 2560 1153
Size: 500 907 2693
Fragments arranged by size:
2693 907 500
PasI CC'CwG_GG
Cuts at: 216 466 216
Size: 250 3850
PciI A'CATG_T
Cuts at: 2556 2556
Size: 4100
PflMI CCAn_nnn'nTGG
Cuts at: 256 256
Size: 4100
PmeI GTTT'AAAC
Cuts at: 1099 1099
Size: 4100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3239 3271 3239
Size: 32 4068
PspOMI G'GGCC_C
Cuts at: 752 817 752
Size: 65 4035
PstI C_TGCA'G
Cuts at: 571 695 1004 1147 1832 571
Size: 124 309 143 685 2839
Fragments arranged by size:
2839 685 309 143 124
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4100
PvuII CAG'CTG
Cuts at: 690 1525 1885 690
Size: 835 360 2905
Fragments arranged by size:
2905 835 360
RsrII CG'GwC_CG
Cuts at: 2295 2295
Size: 4100
SacI G_AGCT'C
Cuts at: 1114 1114
Size: 4100
SalI G'TCGA_C
Cuts at: 1078 1137 1078
Size: 59 4041
SapI GCTCTTCn'nnn_
Cuts at: 805 1011 2123 2333 805
Size: 206 1112 210 2572
Fragments arranged by size:
2572 1112 210 206
SbfI CC_TGCA'GG
Cuts at: 1004 1147 1004
Size: 143 3957
ScaI AGT'ACT
Cuts at: 3617 3617
Size: 4100
SfcI C'TryA_G
Cuts at: 33 567 691 1000 1143 1828 2821 3012 33
Size: 534 124 309 143 685 993 191 1121
Fragments arranged by size:
1121 993 685 534 309 191 143 124
SfoI GGC'GCC
Cuts at: 1532 1780 1532
Size: 248 3852
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4100
SmaI CCC'GGG
Cuts at: 751 1027 1122 751
Size: 276 95 3729
Fragments arranged by size:
3729 276 95
SmlI C'TyrA_G
Cuts at: 1255 2170 2662 2924 1255
Size: 915 492 262 2431
Fragments arranged by size:
2431 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4100
SphI G_CATG'C
Cuts at: 1153 1653 1153
Size: 500 3600
SrfI GCCC'GGGC
Cuts at: 751 751
Size: 4100
StyI C'CwwG_G
Cuts at: 149 906 941 1234 1588 149
Size: 757 35 293 354 2661
Fragments arranged by size:
2661 757 354 293 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 628 1073 1683 1816 2275 2484 628
Size: 445 610 133 459 209 2244
Fragments arranged by size:
2244 610 459 445 209 133
TatI w'GTAC_w
Cuts at: 3615 3615
Size: 4100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 543 575 3617 3649 543
Size: 32 3042 32 994
Fragments arranged by size:
3042 994 32 32
Tth111I GACn'n_nGTC
Cuts at: 1897 1897
Size: 4100
XbaI T'CTAG_A
Cuts at: 1131 1131
Size: 4100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 156 414 553 1595 156
Size: 258 139 1042 2661
Fragments arranged by size:
2661 1042 258 139
XmaI C'CCGG_G
Cuts at: 749 1025 1120 749
Size: 276 95 3729
Fragments arranged by size:
3729 276 95
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI BaeI
BbsI BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SspI StuI SwaI XhoI XmnI ZraI