Restriction map for
pF1KB8993
(Circular) (Six-base) MAPSORT of: pF1KB8993.seq Check: 6246 from: 1 to: 4316
pF1KB8993 4316 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1354 1354
Size: 4316
Acc65I G'GTAC_C
Cuts at: 1332 1332
Size: 4316
AclI AA'CG_TT
Cuts at: 1310 4156 1310
Size: 2846 1470
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 107 149 821 2141 2573 3320 3588 107
Size: 42 672 1320 432 747 268 835
Fragments arranged by size:
1320 835 747 672 432 268 42
AflII C'TTAA_G
Cuts at: 612 612
Size: 4316
AflIII A'CryG_T
Cuts at: 114 1668 2772 3749 114
Size: 1554 1104 977 681
Fragments arranged by size:
1554 1104 977 681
AgeI A'CCGG_T
Cuts at: 2704 2704
Size: 4316
AhdI GACnn_n'nnGTC
Cuts at: 225 225
Size: 4316
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 188 220 188
Size: 32 4284
AlwNI CAG_nnn'CTG
Cuts at: 1621 3188 1621
Size: 1567 2749
ApaLI G'TGCA_C
Cuts at: 3086 3086
Size: 4316
ApoI r'AATT_y
Cuts at: 452 542 552 642 837 888 1053 1320 3669
Size: 90 10 90 195 51 165 267 2349
Cuts at: 3669 3925 452
Size: 256 843
Fragments arranged by size:
2349 843 267 256 195 165 90 90
51 10
AseI AT'TA_AT
Cuts at: 20 428 20
Size: 408 3908
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4316
AvaI C'yCGr_G
Cuts at: 1336 1336
Size: 4316
BamHI G'GATC_C
Cuts at: 268 1341 268
Size: 1073 3243
BanI G'GyrC_C
Cuts at: 1332 1746 1994 2029 1332
Size: 414 248 35 3619
Fragments arranged by size:
3619 414 248 35
BanII G_rGCy'C
Cuts at: 1330 2360 1330
Size: 1030 3286
BbeI G_GCGC'C
Cuts at: 1750 1998 1750
Size: 248 4068
BciVI GTATCCnnnnn_n'
Cuts at: 2214 2975 4316 2214
Size: 761 1341 2214
Fragments arranged by size:
2214 1341 761
BclI T'GATC_A
Cuts at: 1836 1836
Size: 4316
BglII A'GATC_T
Cuts at: 562 1831 3868 562
Size: 1269 2037 1010
Fragments arranged by size:
2037 1269 1010
BlpI GC'TnA_GC
Cuts at: 1428 1428
Size: 4316
Bme1580I G_kGCm'C
Cuts at: 1941 2034 3090 1941
Size: 93 1056 3167
Fragments arranged by size:
3167 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1528 1609 1944 4088 1528
Size: 81 335 2144 1756
Fragments arranged by size:
2144 1756 335 81
BmtI G_CTAG'C
Cuts at: 1267 1267
Size: 4316
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2683 2683
Size: 4316
Bpu10I CC'TnA_GC
Cuts at: 792 792
Size: 4316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 116 1492 2371 2863 3161 116
Size: 1376 879 492 298 1271
Fragments arranged by size:
1376 1271 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 188 4020 188
Size: 3832 484
BsaAI yAC'GTr
Cuts at: 1669 1669
Size: 4316
BsaBI GATnn'nnATC
Cuts at: 6 1830 3867 6
Size: 1824 2037 455
Fragments arranged by size:
2037 1824 455
BsaHI Gr'CG_yC
Cuts at: 1747 1995 2697 4193 1747
Size: 248 702 1496 1870
Fragments arranged by size:
1870 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1505 2026 2704 2978 3125 3536 1505
Size: 521 678 274 147 411 2285
Fragments arranged by size:
2285 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 168 198 453 483 168
Size: 30 255 30 4001
Fragments arranged by size:
4001 255 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 911 911
Size: 4316
BseYI C'CCAG_C
Cuts at: 1741 2499 3076 3572 1741
Size: 758 577 496 2485
Fragments arranged by size:
2485 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1232 1858 1904 3112 104
Size: 1128 626 46 1208 1308
Fragments arranged by size:
1308 1208 1128 626 46
BsiHKAI G_wGCw'C
Cuts at: 1330 2108 2298 3090 1330
Size: 778 190 792 2556
Fragments arranged by size:
2556 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1330 1941 2034 2108 2298 2360 3090 1330
Size: 611 93 74 190 62 730 2556
Fragments arranged by size:
2556 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3536 3536
Size: 4316
BspHI T'CATG_A
Cuts at: 3492 3492
Size: 4316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1366 1580 1882 2263 1366
Size: 214 302 381 3419
Fragments arranged by size:
3419 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2608 2662 4146 4311 50
Size: 2558 54 1484 165 55
Fragments arranged by size:
2558 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2228 2228
Size: 4316
BsrFI r'CCGG_y
Cuts at: 2314 2495 2704 2314
Size: 181 209 3926
Fragments arranged by size:
3926 209 181
BssSI C'ACGA_G
Cuts at: 2587 2945 2587
Size: 358 3958
BstAPI GCAn_nnn'nTGC
Cuts at: 1693 1693
Size: 4316
BstBI TT'CG_AA
Cuts at: 2677 2677
Size: 4316
BstYI r'GATC_y
Cuts at: 1 268 562 1341 1808 1831 2166 2412 3424
Size: 267 294 779 467 23 335 246 1012
Cuts at: 3424 3510 3522 3868 1
Size: 86 12 346 449
Fragments arranged by size:
1012 779 467 449 346 335 294 267
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 1210 107
Size: 1103 3213
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2266 2447 2610 2266
Size: 181 163 3972
Fragments arranged by size:
3972 181 163
BtsI GCAGTG_nn'
Cuts at: 1559 1559
Size: 4316
DraI TTT'AAA
Cuts at: 842 986 1315 842
Size: 144 329 3843
Fragments arranged by size:
3843 329 144
DraIII CAC_nnn'GTG
Cuts at: 1050 1050
Size: 4316
DrdI GACnn_nn'nnGTC
Cuts at: 2022 2880 2022
Size: 858 3458
EaeI y'GGCC_r
Cuts at: 1901 2075 2466 2493 3838 1901
Size: 174 391 27 1345 2379
Fragments arranged by size:
2379 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1901 1901
Size: 4316
EarI CTCTTCn'nnn_
Cuts at: 445 587 676 1274 2339 2549 445
Size: 142 89 598 1065 210 2212
Fragments arranged by size:
2212 1065 598 210 142 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2524 2834 2980 4126 2524
Size: 310 146 1146 2714
Fragments arranged by size:
2714 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1328 1328
Size: 4316
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 107 149 821 2141 2573 3320 3588 107
Size: 42 672 1320 432 747 268 835
Fragments arranged by size:
1320 835 747 672 432 268 42
EcoNI CCTnn'n_nnAGG
Cuts at: 177 177
Size: 4316
EcoO109I rG'GnC_Cy
Cuts at: 1455 1455
Size: 4316
EcoRI G'AATT_C
Cuts at: 1320 1320
Size: 4316
EcoRV GAT'ATC
Cuts at: 1153 1153
Size: 4316
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 881 2335 881
Size: 1454 2862
FspI TGC'GCA
Cuts at: 2097 2097
Size: 4316
HaeII r_GCGC'y
Cuts at: 1750 1998 3020 3999 1750
Size: 248 1022 979 2067
Fragments arranged by size:
2067 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 211 243 586 618 2158 2188 2190 2220 211
Size: 32 343 32 1540 30 2 30 2307
Fragments arranged by size:
2307 1540 343 32 32 30 30 2
HincII GTy'rAC
Cuts at: 995 1355 995
Size: 360 3956
KasI G'GCGC_C
Cuts at: 1746 1994 1746
Size: 248 4068
KpnI G_GTAC'C
Cuts at: 1336 1336
Size: 4316
MluI A'CGCG_T
Cuts at: 3749 3749
Size: 4316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 924 2987 3171 924
Size: 2063 184 2069
Fragments arranged by size:
2069 2063 184
MscI TGG'CCA
Cuts at: 2077 2077
Size: 4316
MslI CAynn'nnrTG
Cuts at: 2305 2432 2305
Size: 127 4189
MspA1I CmG'CkG
Cuts at: 1246 1427 1741 2101 3114 3359 1246
Size: 181 314 360 1013 245 2203
Fragments arranged by size:
2203 1013 360 314 245 181
NaeI GCC'GGC
Cuts at: 2497 2497
Size: 4316
NarI GG'CG_CC
Cuts at: 1747 1995 1747
Size: 248 4068
NcoI C'CATG_G
Cuts at: 107 107
Size: 4316
NdeI CA'TA_TG
Cuts at: 1007 1007
Size: 4316
NgoMIV G'CCGG_C
Cuts at: 2495 2495
Size: 4316
NheI G'CTAG_C
Cuts at: 1263 1263
Size: 4316
NspI r_CATG'y
Cuts at: 118 247 979 1032 1194 1369 1869 2776 118
Size: 129 732 53 162 175 500 907 1658
Fragments arranged by size:
1658 907 732 500 175 162 129 53
PasI CC'CwG_GG
Cuts at: 208 208
Size: 4316
PciI A'CATG_T
Cuts at: 114 2772 114
Size: 2658 1658
PmeI GTTT'AAAC
Cuts at: 1315 1315
Size: 4316
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3455 3487 3455
Size: 32 4284
PsiI TTA'TAA
Cuts at: 635 635
Size: 4316
PstI C_TGCA'G
Cuts at: 1363 2048 1363
Size: 685 3631
PvuI CG_AT'CG
Cuts at: 104 1232 104
Size: 1128 3188
PvuII CAG'CTG
Cuts at: 1246 1741 2101 1246
Size: 495 360 3461
Fragments arranged by size:
3461 495 360
RsrII CG'GwC_CG
Cuts at: 2511 2511
Size: 4316
SacI G_AGCT'C
Cuts at: 1330 1330
Size: 4316
SalI G'TCGA_C
Cuts at: 1353 1353
Size: 4316
SapI GCTCTTCn'nnn_
Cuts at: 1274 2339 2549 1274
Size: 1065 210 3041
Fragments arranged by size:
3041 1065 210
SbfI CC_TGCA'GG
Cuts at: 1363 1363
Size: 4316
ScaI AGT'ACT
Cuts at: 3833 3833
Size: 4316
SfcI C'TryA_G
Cuts at: 33 1359 2044 3037 3228 33
Size: 1326 685 993 191 1121
Fragments arranged by size:
1326 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1748 1996 1748
Size: 248 4068
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4316
SmaI CCC'GGG
Cuts at: 1338 1338
Size: 4316
SmlI C'TyrA_G
Cuts at: 131 612 1471 2386 2878 3140 131
Size: 481 859 915 492 262 1307
Fragments arranged by size:
1307 915 859 492 481 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4316
SphI G_CATG'C
Cuts at: 1369 1869 1369
Size: 500 3816
SspI AAT'ATT
Cuts at: 482 482
Size: 4316
StyI C'CwwG_G
Cuts at: 107 1450 1804 107
Size: 1343 354 2619
Fragments arranged by size:
2619 1343 354
SwaI ATTT'AAAT
Cuts at: 842 842
Size: 4316
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1899 2032 2491 2700 1899
Size: 133 459 209 3515
Fragments arranged by size:
3515 459 209 133
TatI w'GTAC_w
Cuts at: 3831 3831
Size: 4316
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 168 200 1137 1169 3833 3865 168
Size: 32 937 32 2664 32 619
Fragments arranged by size:
2664 937 619 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2113 2113
Size: 4316
XbaI T'CTAG_A
Cuts at: 1347 1347
Size: 4316
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1811 1811
Size: 4316
XmaI C'CCGG_G
Cuts at: 1336 1336
Size: 4316
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NspI PasI PciI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AleI AlfI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BpmI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI FseI FspAI HindIII HpaI
MfeI NotI NruI NsiI PacI PflMI PfoI PmlI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI XhoI XmnI ZraI