Restriction map for
pF1KB8989
(Circular) (Six-base) MAPSORT of: pF1KB8989.seq Check: 9722 from: 1 to: 4283
pF1KB8989 4283 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AatII G_ACGT'C
Cuts at: 985 985
Size: 4283
AccI GT'mk_AC
Cuts at: 302 1052 1321 302
Size: 750 269 3264
Fragments arranged by size:
3264 750 269
Acc65I G'GTAC_C
Cuts at: 152 1299 152
Size: 1147 3136
AclI AA'CG_TT
Cuts at: 4123 4123
Size: 4283
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 997 2108 2540 3287 3555 997
Size: 1111 432 747 268 1725
Fragments arranged by size:
1725 1111 747 432 268
AflIII A'CryG_T
Cuts at: 1635 2739 3716 1635
Size: 1104 977 2202
Fragments arranged by size:
2202 1104 977
AgeI A'CCGG_T
Cuts at: 2671 2671
Size: 4283
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 799 799
Size: 4283
AlwNI CAG_nnn'CTG
Cuts at: 1588 3155 1588
Size: 1567 2716
ApaLI G'TGCA_C
Cuts at: 1213 3053 1213
Size: 1840 2443
ApoI r'AATT_y
Cuts at: 1287 3636 3892 1287
Size: 2349 256 1678
Fragments arranged by size:
2349 1678 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4283
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4283
AvaI C'yCGr_G
Cuts at: 148 682 1303 148
Size: 534 621 3128
Fragments arranged by size:
3128 621 534
BamHI G'GATC_C
Cuts at: 1308 1308
Size: 4283
BanI G'GyrC_C
Cuts at: 152 548 951 1059 1141 1299 1713 1961 1996
Size: 396 403 108 82 158 414 248 35
Cuts at: 1996 152
Size: 2439
Fragments arranged by size:
2439 414 403 396 248 158 108 82
35
BanII G_rGCy'C
Cuts at: 195 1297 2327 195
Size: 1102 1030 2151
Fragments arranged by size:
2151 1102 1030
BbeI G_GCGC'C
Cuts at: 552 1145 1717 1965 552
Size: 593 572 248 2870
Fragments arranged by size:
2870 593 572 248
BbsI GAAGACnn'nnnn_
Cuts at: 478 841 874 907 985 478
Size: 363 33 33 78 3776
Fragments arranged by size:
3776 363 78 33 33
BciVI GTATCCnnnnn_n'
Cuts at: 2181 2942 4283 2181
Size: 761 1341 2181
Fragments arranged by size:
2181 1341 761
BclI T'GATC_A
Cuts at: 236 1803 236
Size: 1567 2716
BglII A'GATC_T
Cuts at: 1798 3835 1798
Size: 2037 2246
BlpI GC'TnA_GC
Cuts at: 271 385 1395 271
Size: 114 1010 3159
Fragments arranged by size:
3159 1010 114
Bme1580I G_kGCm'C
Cuts at: 943 956 1217 1908 2001 3057 943
Size: 13 261 691 93 1056 2169
Fragments arranged by size:
2169 1056 691 261 93 13
BmgBI CAC'GTC
Cuts at: 1080 1212 1080
Size: 132 4151
BmrI ACTGGGnnnn_n'
Cuts at: 1495 1576 1911 4055 1495
Size: 81 335 2144 1723
Fragments arranged by size:
2144 1723 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 198 2650 198
Size: 2452 1831
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 460 511 541 724 883 916 937 1246
Size: 108 51 30 183 159 33 21 309
Cuts at: 1246 352
Size: 3389
Fragments arranged by size:
3389 309 183 159 108 51 33 30
21
Bpu10I CC'TnA_GC
Cuts at: 194 194
Size: 4283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 305 862 1099 1459 2338 2830 3128 305
Size: 557 237 360 879 492 298 1460
Fragments arranged by size:
1460 879 557 492 360 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 3987 3987
Size: 4283
BsaAI yAC'GTr
Cuts at: 1023 1636 1023
Size: 613 3670
BsaBI GATnn'nnATC
Cuts at: 6 1797 3834 6
Size: 1791 2037 455
Fragments arranged by size:
2037 1791 455
BsaHI Gr'CG_yC
Cuts at: 161 549 982 1142 1247 1714 1962 2664 4160
Size: 388 433 160 105 467 248 702 1496
Cuts at: 4160 161
Size: 284
Fragments arranged by size:
1496 702 467 433 388 284 248 160
105
BsaWI w'CCGG_w
Cuts at: 1472 1993 2671 2945 3092 3503 1472
Size: 521 678 274 147 411 2252
Fragments arranged by size:
2252 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 196 226 697 727 1021 1051 196
Size: 30 471 30 294 30 3428
Fragments arranged by size:
3428 471 294 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 349 673 700 751 763 1252 220
Size: 129 324 27 51 12 489 3251
Fragments arranged by size:
3251 489 324 129 51 27 12
BseYI C'CCAG_C
Cuts at: 187 393 663 936 1708 2466 3043 3539 187
Size: 206 270 273 772 758 577 496 931
Fragments arranged by size:
931 772 758 577 496 273 270 206
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 385
Size: 4283
BsiEI CG_ry'CG
Cuts at: 104 142 1825 1871 3079 104
Size: 38 1683 46 1208 1308
Fragments arranged by size:
1683 1308 1208 46 38
BsiHKAI G_wGCw'C
Cuts at: 1115 1217 1297 2075 2265 3057 1115
Size: 102 80 778 190 792 2341
Fragments arranged by size:
2341 792 778 190 102 80
Bsp1286I G_dGCh'C
Cuts at: 195 943 956 1115 1217 1297 1908 2001 2075
Size: 748 13 159 102 80 611 93 74
Cuts at: 2075 2265 2327 3057 195
Size: 190 62 730 1421
Fragments arranged by size:
1421 748 730 611 190 159 102 93
80 74 62 13
BspEI T'CCGG_A
Cuts at: 3503 3503
Size: 4283
BspHI T'CATG_A
Cuts at: 566 3459 566
Size: 2893 1390
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1196 1333 1547 1849 2230 1196
Size: 137 214 302 381 3249
Fragments arranged by size:
3249 381 302 214 137
BsrBI CCG'CTC
Cuts at: 50 138 339 678 963 2575 2629 4113 4278
Size: 88 201 339 285 1612 54 1484 165
Cuts at: 4278 50
Size: 55
Fragments arranged by size:
1612 1484 339 285 201 165 88 55
54
BsrDI GCAATG_nn'
Cuts at: 2195 2195
Size: 4283
BsrFI r'CCGG_y
Cuts at: 220 594 1180 2281 2462 2671 220
Size: 374 586 1101 181 209 1832
Fragments arranged by size:
1832 1101 586 374 209 181
BssHII G'CGCG_C
Cuts at: 1033 1033
Size: 4283
BssSI C'ACGA_G
Cuts at: 414 2554 2912 414
Size: 2140 358 1785
Fragments arranged by size:
2140 1785 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1221 1660 1221
Size: 439 3844
BstBI TT'CG_AA
Cuts at: 2644 2644
Size: 4283
BstXI CCAn_nnnn'nTGG
Cuts at: 1128 1128
Size: 4283
BstYI r'GATC_y
Cuts at: 1 308 1088 1308 1775 1798 2133 2379 3391
Size: 307 780 220 467 23 335 246 1012
Cuts at: 3391 3477 3489 3835 1
Size: 86 12 346 449
Fragments arranged by size:
1012 780 467 449 346 335 307 246
220 86 23 12
BtgI C'CryG_G
Cuts at: 107 1046 107
Size: 939 3344
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2233 2414 2577 2233
Size: 181 163 3939
Fragments arranged by size:
3939 181 163
BtsI GCAGTG_nn'
Cuts at: 368 1526 368
Size: 1158 3125
DraI TTT'AAA
Cuts at: 561 1282 561
Size: 721 3562
DraIII CAC_nnn'GTG
Cuts at: 1026 1026
Size: 4283
DrdI GACnn_nn'nnGTC
Cuts at: 1989 2847 1989
Size: 858 3425
EaeI y'GGCC_r
Cuts at: 139 596 1868 2042 2433 2460 3805 139
Size: 457 1272 174 391 27 1345 617
Fragments arranged by size:
1345 1272 617 457 391 174 27
EagI C'GGCC_G
Cuts at: 139 1868 139
Size: 1729 2554
EarI CTCTTCn'nnn_
Cuts at: 224 2306 2516 224
Size: 2082 210 1991
Fragments arranged by size:
2082 1991 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 361 2491 2801 2947 4093 361
Size: 2130 310 146 1146 551
Fragments arranged by size:
2130 1146 551 310 146
EcoICRI GAG'CTC
Cuts at: 1295 1295
Size: 4283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 460 511 541 724 883 916 937 997
Size: 108 51 30 183 159 33 21 60
Cuts at: 997 1246 2108 2540 3287 3555 352
Size: 249 862 432 747 268 1080
Fragments arranged by size:
1080 862 747 432 268 249 183 159
108 60 51 33 30 21
EcoO109I rG'GnC_Cy
Cuts at: 114 168 1422 114
Size: 54 1254 2975
Fragments arranged by size:
2975 1254 54
EcoRI G'AATT_C
Cuts at: 1287 1287
Size: 4283
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 828 2302 828
Size: 1474 2809
FspI TGC'GCA
Cuts at: 451 1000 2064 451
Size: 549 1064 2670
Fragments arranged by size:
2670 1064 549
HaeII r_GCGC'y
Cuts at: 498 552 640 703 1145 1717 1965 2987 3966
Size: 54 88 63 442 572 248 1022 979
Cuts at: 3966 498
Size: 815
Fragments arranged by size:
1022 979 815 572 442 248 88 63
54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 196 228 697 729 2125 2155 2157 2187 196
Size: 32 469 32 1396 30 2 30 2292
Fragments arranged by size:
2292 1396 469 32 32 30 30 2
HincII GTy'rAC
Cuts at: 465 528 1322 465
Size: 63 794 3426
Fragments arranged by size:
3426 794 63
KasI G'GCGC_C
Cuts at: 548 1141 1713 1961 548
Size: 593 572 248 2870
Fragments arranged by size:
2870 593 572 248
KpnI G_GTAC'C
Cuts at: 156 1303 156
Size: 1147 3136
MluI A'CGCG_T
Cuts at: 3716 3716
Size: 4283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 224 611 695 1223 2954 3138 224
Size: 387 84 528 1731 184 1369
Fragments arranged by size:
1731 1369 528 387 184 84
MscI TGG'CCA
Cuts at: 2044 2044
Size: 4283
MslI CAynn'nnrTG
Cuts at: 1126 2272 2399 1126
Size: 1146 127 3010
Fragments arranged by size:
3010 1146 127
MspA1I CmG'CkG
Cuts at: 187 202 315 330 697 779 1048 1394 1708
Size: 15 113 15 367 82 269 346 314
Cuts at: 1708 2068 3081 3326 187
Size: 360 1013 245 1144
Fragments arranged by size:
1144 1013 367 360 346 314 269 245
113 82 15 15
NaeI GCC'GGC
Cuts at: 222 596 1182 2464 222
Size: 374 586 1282 2041
Fragments arranged by size:
2041 1282 586 374
NarI GG'CG_CC
Cuts at: 549 1142 1714 1962 549
Size: 593 572 248 2870
Fragments arranged by size:
2870 593 572 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4283
NgoMIV G'CCGG_C
Cuts at: 220 594 1180 2462 220
Size: 374 586 1282 2041
Fragments arranged by size:
2041 1282 586 374
NotI GC'GGCC_GC
Cuts at: 139 139
Size: 4283
NspI r_CATG'y
Cuts at: 1336 1836 2743 1336
Size: 500 907 2876
Fragments arranged by size:
2876 907 500
PciI A'CATG_T
Cuts at: 2739 2739
Size: 4283
PfoI T'CCnGG_A
Cuts at: 278 278
Size: 4283
PmeI GTTT'AAAC
Cuts at: 1282 1282
Size: 4283
PmlI CAC'GTG
Cuts at: 1023 1023
Size: 4283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3422 3454 3422
Size: 32 4251
PpuMI rG'GwC_Cy
Cuts at: 114 114
Size: 4283
PshAI GACnn'nnGTC
Cuts at: 1048 1048
Size: 4283
PstI C_TGCA'G
Cuts at: 1330 2015 1330
Size: 685 3598
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4283
PvuII CAG'CTG
Cuts at: 315 330 1708 2068 315
Size: 15 1378 360 2530
Fragments arranged by size:
2530 1378 360 15
RsrII CG'GwC_CG
Cuts at: 2478 2478
Size: 4283
SacI G_AGCT'C
Cuts at: 1297 1297
Size: 4283
SacII CC_GC'GG
Cuts at: 1049 1049
Size: 4283
SalI G'TCGA_C
Cuts at: 1320 1320
Size: 4283
SapI GCTCTTCn'nnn_
Cuts at: 224 2306 2516 224
Size: 2082 210 1991
Fragments arranged by size:
2082 1991 210
SbfI CC_TGCA'GG
Cuts at: 1330 1330
Size: 4283
ScaI AGT'ACT
Cuts at: 3800 3800
Size: 4283
SexAI A'CCwGG_T
Cuts at: 248 248
Size: 4283
SfcI C'TryA_G
Cuts at: 33 1326 2011 3004 3195 33
Size: 1293 685 993 191 1121
Fragments arranged by size:
1293 1121 993 685 191
SfoI GGC'GCC
Cuts at: 550 1143 1715 1963 550
Size: 593 572 248 2870
Fragments arranged by size:
2870 593 572 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4283
SmaI CCC'GGG
Cuts at: 150 1305 150
Size: 1155 3128
SmlI C'TyrA_G
Cuts at: 320 877 1114 1438 2353 2845 3107 320
Size: 557 237 324 915 492 262 1496
Fragments arranged by size:
1496 915 557 492 324 262 237
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4283
SphI G_CATG'C
Cuts at: 1336 1836 1336
Size: 500 3783
StuI AGG'CCT
Cuts at: 811 811
Size: 4283
StyI C'CwwG_G
Cuts at: 107 818 839 884 1417 1771 107
Size: 711 21 45 533 354 2619
Fragments arranged by size:
2619 711 533 354 45 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1866 1999 2458 2667 1866
Size: 133 459 209 3482
Fragments arranged by size:
3482 459 209 133
TatI w'GTAC_w
Cuts at: 3798 3798
Size: 4283
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3800 3832 3800
Size: 32 4251
Tth111I GACn'n_nGTC
Cuts at: 2080 2080
Size: 4283
XbaI T'CTAG_A
Cuts at: 1314 1314
Size: 4283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 825 891 1128 1778 825
Size: 66 237 650 3330
Fragments arranged by size:
3330 650 237 66
XmaI C'CCGG_G
Cuts at: 148 1303 148
Size: 1155 3128
XmnI GAAnn'nnTTC
Cuts at: 811 811
Size: 4283
ZraI GAC'GTC
Cuts at: 983 983
Size: 4283
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI BsiWI
BsmI BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NruI NsiI PacI PasI PflMI PsiI PspOMI
PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI