Restriction map for
pF1KB8988
(Circular) (Six-base) MAPSORT of: pF1KB8988.seq Check: 7065 from: 1 to: 4277
pF1KB8988 4277 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 824 824
Size: 4277
AccI GT'mk_AC
Cuts at: 1315 1315
Size: 4277
Acc65I G'GTAC_C
Cuts at: 1293 1293
Size: 4277
AclI AA'CG_TT
Cuts at: 4117 4117
Size: 4277
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2102 2534 3281 3549 2102
Size: 432 747 268 2830
Fragments arranged by size:
2830 747 432 268
AflIII A'CryG_T
Cuts at: 692 1629 2733 3710 692
Size: 937 1104 977 1259
Fragments arranged by size:
1259 1104 977 937
AgeI A'CCGG_T
Cuts at: 2665 2665
Size: 4277
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 634 634
Size: 4277
AlwNI CAG_nnn'CTG
Cuts at: 1582 3149 1582
Size: 1567 2710
ApaLI G'TGCA_C
Cuts at: 3047 3047
Size: 4277
ApoI r'AATT_y
Cuts at: 1099 1281 3630 3886 1099
Size: 182 2349 256 1490
Fragments arranged by size:
2349 1490 256 182
AseI AT'TA_AT
Cuts at: 20 20
Size: 4277
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4277
AvaI C'yCGr_G
Cuts at: 139 148 213 498 524 678 1297 139
Size: 9 65 285 26 154 619 3119
Fragments arranged by size:
3119 619 285 154 65 26 9
BamHI G'GATC_C
Cuts at: 1302 1302
Size: 4277
BanI G'GyrC_C
Cuts at: 673 824 1293 1707 1955 1990 673
Size: 151 469 414 248 35 2960
Fragments arranged by size:
2960 469 414 248 151 35
BanII G_rGCy'C
Cuts at: 229 332 517 883 1291 2321 229
Size: 103 185 366 408 1030 2185
Fragments arranged by size:
2185 1030 408 366 185 103
BbeI G_GCGC'C
Cuts at: 1711 1959 1711
Size: 248 4029
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1083 1117 1083
Size: 34 4243
BciVI GTATCCnnnnn_n'
Cuts at: 250 2175 2936 4277 250
Size: 1925 761 1341 250
Fragments arranged by size:
1925 1341 761 250
BclI T'GATC_A
Cuts at: 1797 1797
Size: 4277
BglI GCCn_nnn'nGGC
Cuts at: 632 1147 632
Size: 515 3762
BglII A'GATC_T
Cuts at: 1792 3829 1792
Size: 2037 2240
BlpI GC'TnA_GC
Cuts at: 331 1389 331
Size: 1058 3219
Bme1580I G_kGCm'C
Cuts at: 157 827 1902 1995 3051 157
Size: 670 1075 93 1056 1383
Fragments arranged by size:
1383 1075 1056 670 93
BmgBI CAC'GTC
Cuts at: 741 741
Size: 4277
BmrI ACTGGGnnnn_n'
Cuts at: 1489 1570 1905 4049 1489
Size: 81 335 2144 1717
Fragments arranged by size:
2144 1717 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2644 2644
Size: 4277
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 766
Size: 4277
Bpu10I CC'TnA_GC
Cuts at: 1028 1028
Size: 4277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1453 2332 2824 3122 1453
Size: 879 492 298 2608
Fragments arranged by size:
2608 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 151 1182 1201 3981 151
Size: 1031 19 2780 447
Fragments arranged by size:
2780 1031 447 19
BsaAI yAC'GTr
Cuts at: 390 695 1247 1630 390
Size: 305 552 383 3037
Fragments arranged by size:
3037 552 383 305
BsaBI GATnn'nnATC
Cuts at: 6 1791 3828 6
Size: 1785 2037 455
Fragments arranged by size:
2037 1785 455
BsaHI Gr'CG_yC
Cuts at: 167 908 1046 1708 1956 2658 4154 167
Size: 741 138 662 248 702 1496 290
Fragments arranged by size:
1496 741 702 662 290 248 138
BsaWI w'CCGG_w
Cuts at: 837 1173 1466 1987 2665 2939 3086 3497 837
Size: 336 293 521 678 274 147 411 1617
Fragments arranged by size:
1617 678 521 411 336 293 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 211 241 1120 1150 211
Size: 30 879 30 3338
Fragments arranged by size:
3338 879 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 759 799 759
Size: 40 4237
BseYI C'CCAG_C
Cuts at: 120 342 573 622 1702 2460 3037 3533 120
Size: 222 231 49 1080 758 577 496 864
Fragments arranged by size:
1080 864 758 577 496 231 222 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 322 301
Size: 21 4256
BsiEI CG_ry'CG
Cuts at: 104 200 454 1819 1865 3073 104
Size: 96 254 1365 46 1208 1308
Fragments arranged by size:
1365 1308 1208 254 96 46
BsiHKAI G_wGCw'C
Cuts at: 332 517 1034 1291 2069 2259 3051 332
Size: 185 517 257 778 190 792 1558
Fragments arranged by size:
1558 792 778 517 257 190 185
BsiWI C'GTAC_G
Cuts at: 386 701 386
Size: 315 3962
BsmBI CGTCTCn'nnnn_
Cuts at: 915 915
Size: 4277
Bsp1286I G_dGCh'C
Cuts at: 157 229 332 517 827 883 1034 1291 1902
Size: 72 103 185 310 56 151 257 611
Cuts at: 1902 1995 2069 2259 2321 3051 157
Size: 93 74 190 62 730 1383
Fragments arranged by size:
1383 730 611 310 257 190 185 151
103 93 74 72 62 56
BspEI T'CCGG_A
Cuts at: 3497 3497
Size: 4277
BspHI T'CATG_A
Cuts at: 3453 3453
Size: 4277
BspMI ACCTGCnnnn'nnnn_
Cuts at: 323 824 1327 1541 1843 2224 323
Size: 501 503 214 302 381 2376
Fragments arranged by size:
2376 503 501 381 302 214
BsrBI CCG'CTC
Cuts at: 50 405 2569 2623 4107 4272 50
Size: 355 2164 54 1484 165 55
Fragments arranged by size:
2164 1484 355 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1101 2189 1101
Size: 1088 3189
BsrFI r'CCGG_y
Cuts at: 195 449 651 2275 2456 2665 195
Size: 254 202 1624 181 209 1807
Fragments arranged by size:
1807 1624 254 209 202 181
BssSI C'ACGA_G
Cuts at: 643 2548 2906 643
Size: 1905 358 2014
Fragments arranged by size:
2014 1905 358
BstAPI GCAn_nnn'nTGC
Cuts at: 423 1654 423
Size: 1231 3046
BstBI TT'CG_AA
Cuts at: 2638 2638
Size: 4277
BstYI r'GATC_y
Cuts at: 1 1302 1769 1792 2127 2373 3385 3471 3483
Size: 1301 467 23 335 246 1012 86 12
Cuts at: 3483 3829 1
Size: 346 449
Fragments arranged by size:
1301 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 1151 1238 107
Size: 1044 87 3146
Fragments arranged by size:
3146 1044 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2227 2408 2571 2227
Size: 181 163 3933
Fragments arranged by size:
3933 181 163
BtsI GCAGTG_nn'
Cuts at: 305 611 690 1520 305
Size: 306 79 830 3062
Fragments arranged by size:
3062 830 306 79
DraI TTT'AAA
Cuts at: 1276 1276
Size: 4277
DrdI GACnn_nn'nnGTC
Cuts at: 1983 2841 1983
Size: 858 3419
EaeI y'GGCC_r
Cuts at: 197 451 792 894 965 1125 1241 1862 2036
Size: 254 341 102 71 160 116 621 174
Cuts at: 2036 2427 2454 3799 197
Size: 391 27 1345 675
Fragments arranged by size:
1345 675 621 391 341 254 174 160
116 102 71 27
EagI C'GGCC_G
Cuts at: 197 451 1862 197
Size: 254 1411 2612
Fragments arranged by size:
2612 1411 254
EarI CTCTTCn'nnn_
Cuts at: 373 544 2300 2510 373
Size: 171 1756 210 2140
Fragments arranged by size:
2140 1756 210 171
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 415 1128 2485 2795 2941 4087 415
Size: 713 1357 310 146 1146 605
Fragments arranged by size:
1357 1146 713 605 310 146
EcoICRI GAG'CTC
Cuts at: 330 515 1289 330
Size: 185 774 3318
Fragments arranged by size:
3318 774 185
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 2102 2534 3281 3549 766
Size: 1336 432 747 268 1494
Fragments arranged by size:
1494 1336 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1198 1416 1198
Size: 218 4059
EcoRI G'AATT_C
Cuts at: 1099 1281 1099
Size: 182 4095
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2296 2296
Size: 4277
FspI TGC'GCA
Cuts at: 2058 2058
Size: 4277
HaeII r_GCGC'y
Cuts at: 364 574 1711 1959 2981 3960 364
Size: 210 1137 248 1022 979 681
Fragments arranged by size:
1137 1022 979 681 248 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 172 204 211 243 2119 2149 2151 2181 172
Size: 32 7 32 1876 30 2 30 2268
Fragments arranged by size:
2268 1876 32 32 30 30 7 2
HincII GTy'rAC
Cuts at: 1316 1316
Size: 4277
KasI G'GCGC_C
Cuts at: 1707 1955 1707
Size: 248 4029
KpnI G_GTAC'C
Cuts at: 1297 1297
Size: 4277
MluI A'CGCG_T
Cuts at: 3710 3710
Size: 4277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 158 1013 2948 3132 158
Size: 855 1935 184 1303
Fragments arranged by size:
1935 1303 855 184
MscI TGG'CCA
Cuts at: 794 896 1127 2038 794
Size: 102 231 911 3033
Fragments arranged by size:
3033 911 231 102
MslI CAynn'nnrTG
Cuts at: 2266 2393 2266
Size: 127 4150
MspA1I CmG'CkG
Cuts at: 1388 1702 2062 3075 3320 1388
Size: 314 360 1013 245 2345
Fragments arranged by size:
2345 1013 360 314 245
NaeI GCC'GGC
Cuts at: 197 653 2458 197
Size: 456 1805 2016
Fragments arranged by size:
2016 1805 456
NarI GG'CG_CC
Cuts at: 1708 1956 1708
Size: 248 4029
NcoI C'CATG_G
Cuts at: 107 1151 107
Size: 1044 3233
NgoMIV G'CCGG_C
Cuts at: 195 651 2456 195
Size: 456 1805 2016
Fragments arranged by size:
2016 1805 456
NspI r_CATG'y
Cuts at: 426 1171 1330 1830 2737 426
Size: 745 159 500 907 1966
Fragments arranged by size:
1966 907 745 500 159
PasI CC'CwG_GG
Cuts at: 876 876
Size: 4277
PciI A'CATG_T
Cuts at: 2733 2733
Size: 4277
PflMI CCAn_nnn'nTGG
Cuts at: 876 876
Size: 4277
PmeI GTTT'AAAC
Cuts at: 1276 1276
Size: 4277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3416 3448 3416
Size: 32 4245
PpuMI rG'GwC_Cy
Cuts at: 1198 1198
Size: 4277
PstI C_TGCA'G
Cuts at: 419 1324 2009 419
Size: 905 685 2687
Fragments arranged by size:
2687 905 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4277
PvuII CAG'CTG
Cuts at: 1702 2062 1702
Size: 360 3917
RsrII CG'GwC_CG
Cuts at: 2472 2472
Size: 4277
SacI G_AGCT'C
Cuts at: 332 517 1291 332
Size: 185 774 3318
Fragments arranged by size:
3318 774 185
SalI G'TCGA_C
Cuts at: 1314 1314
Size: 4277
SanDI GG'GwC_CC
Cuts at: 1198 1198
Size: 4277
SapI GCTCTTCn'nnn_
Cuts at: 2300 2510 2300
Size: 210 4067
SbfI CC_TGCA'GG
Cuts at: 1324 1324
Size: 4277
ScaI AGT'ACT
Cuts at: 601 3794 601
Size: 3193 1084
SfcI C'TryA_G
Cuts at: 33 415 864 1260 1320 2005 2998 3189 33
Size: 382 449 396 60 685 993 191 1121
Fragments arranged by size:
1121 993 685 449 396 382 191 60
SfoI GGC'GCC
Cuts at: 1709 1957 1709
Size: 248 4029
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4277
SmaI CCC'GGG
Cuts at: 215 500 526 1299 215
Size: 285 26 773 3193
Fragments arranged by size:
3193 773 285 26
SmlI C'TyrA_G
Cuts at: 148 1432 2347 2839 3101 148
Size: 1284 915 492 262 1324
Fragments arranged by size:
1324 1284 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4277
SphI G_CATG'C
Cuts at: 1330 1830 1330
Size: 500 3777
StyI C'CwwG_G
Cuts at: 107 133 275 891 1151 1411 1765 107
Size: 26 142 616 260 260 354 2619
Fragments arranged by size:
2619 616 354 260 260 142 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 992 1145 1860 1993 2452 2661 992
Size: 153 715 133 459 209 2608
Fragments arranged by size:
2608 715 459 209 153 133
TatI w'GTAC_w
Cuts at: 599 3792 599
Size: 3193 1084
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1120 1152 3794 3826 1120
Size: 32 2642 32 1571
Fragments arranged by size:
2642 1571 32 32
Tth111I GACn'n_nGTC
Cuts at: 369 2074 369
Size: 1705 2572
XbaI T'CTAG_A
Cuts at: 1308 1308
Size: 4277
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 787 1149 1772 787
Size: 362 623 3292
Fragments arranged by size:
3292 623 362
XhoI C'TCGA_G
Cuts at: 148 148
Size: 4277
XmaI C'CCGG_G
Cuts at: 213 498 524 1297 213
Size: 285 26 773 3193
Fragments arranged by size:
3193 773 285 26
XmnI GAAnn'nnTTC
Cuts at: 259 259
Size: 4277
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BmtI BsmI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI ZraI