Restriction map for
pF1KB8985
(Circular) (Six-base) MAPSORT of: pF1KB8985.seq Check: 9892 from: 1 to: 4274
pF1KB8985 4274 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1001 1270 1001
Size: 269 4005
AatII G_ACGT'C
Cuts at: 481 481
Size: 4274
AccI GT'mk_AC
Cuts at: 1312 1312
Size: 4274
Acc65I G'GTAC_C
Cuts at: 1290 1290
Size: 4274
AclI AA'CG_TT
Cuts at: 4114 4114
Size: 4274
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 440 1032 2099 2531 3278 3546 440
Size: 592 1067 432 747 268 1168
Fragments arranged by size:
1168 1067 747 592 432 268
AflIII A'CryG_T
Cuts at: 932 1626 2730 3707 932
Size: 694 1104 977 1499
Fragments arranged by size:
1499 1104 977 694
AgeI A'CCGG_T
Cuts at: 2662 2662
Size: 4274
AlwNI CAG_nnn'CTG
Cuts at: 1579 3146 1579
Size: 1567 2707
ApaLI G'TGCA_C
Cuts at: 1208 3044 1208
Size: 1836 2438
ApoI r'AATT_y
Cuts at: 1278 3627 3883 1278
Size: 2349 256 1669
Fragments arranged by size:
2349 1669 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4274
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4274
AvaI C'yCGr_G
Cuts at: 184 607 828 1294 184
Size: 423 221 466 3164
Fragments arranged by size:
3164 466 423 221
BamHI G'GATC_C
Cuts at: 1299 1299
Size: 4274
BanI G'GyrC_C
Cuts at: 398 444 989 1227 1290 1704 1952 1987 398
Size: 46 545 238 63 414 248 35 2685
Fragments arranged by size:
2685 545 414 248 238 63 46 35
BanII G_rGCy'C
Cuts at: 578 614 721 1288 2318 578
Size: 36 107 567 1030 2534
Fragments arranged by size:
2534 1030 567 107 36
BbeI G_GCGC'C
Cuts at: 1231 1708 1956 1231
Size: 477 248 3549
Fragments arranged by size:
3549 477 248
BciVI GTATCCnnnnn_n'
Cuts at: 539 2172 2933 4274 539
Size: 1633 761 1341 539
Fragments arranged by size:
1633 1341 761 539
BclI T'GATC_A
Cuts at: 1794 1794
Size: 4274
BglI GCCn_nnn'nGGC
Cuts at: 649 876 649
Size: 227 4047
BglII A'GATC_T
Cuts at: 1789 3826 1789
Size: 2037 2237
BlpI GC'TnA_GC
Cuts at: 466 1386 466
Size: 920 3354
Bme1580I G_kGCm'C
Cuts at: 449 940 1212 1899 1992 3048 449
Size: 491 272 687 93 1056 1675
Fragments arranged by size:
1675 1056 687 491 272 93
BmgBI CAC'GTC
Cuts at: 516 516
Size: 4274
BmrI ACTGGGnnnn_n'
Cuts at: 1486 1567 1902 4046 1486
Size: 81 335 2144 1714
Fragments arranged by size:
2144 1714 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2641 2641
Size: 4274
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 811
Size: 4274
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 850 1159 1450 2329 2821 3119 850
Size: 309 291 879 492 298 2005
Fragments arranged by size:
2005 879 492 309 298 291
BsaI GGTCTCn'nnnn_
Cuts at: 787 3978 787
Size: 3191 1083
BsaAI yAC'GTr
Cuts at: 1627 1627
Size: 4274
BsaBI GATnn'nnATC
Cuts at: 6 1788 3825 6
Size: 1782 2037 455
Fragments arranged by size:
2037 1782 455
BsaHI Gr'CG_yC
Cuts at: 478 1228 1705 1953 2655 4151 478
Size: 750 477 248 702 1496 601
Fragments arranged by size:
1496 750 702 601 477 248
BsaWI w'CCGG_w
Cuts at: 726 1463 1984 2662 2936 3083 3494 726
Size: 737 521 678 274 147 411 1506
Fragments arranged by size:
1506 737 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 199 229 307 337 511 541 703 733 199
Size: 30 78 30 174 30 162 30 3740
Fragments arranged by size:
3740 174 162 78 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 202
Size: 4274
BseYI C'CCAG_C
Cuts at: 196 848 1699 2457 3034 3530 196
Size: 652 851 758 577 496 940
Fragments arranged by size:
940 851 758 652 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 517
Size: 4274
BsiEI CG_ry'CG
Cuts at: 104 944 957 1816 1862 3070 104
Size: 840 13 859 46 1208 1308
Fragments arranged by size:
1308 1208 859 840 46 13
BsiHKAI G_wGCw'C
Cuts at: 734 1212 1288 2066 2256 3048 734
Size: 478 76 778 190 792 1960
Fragments arranged by size:
1960 792 778 478 190 76
BsmI GAATG_Cn'
Cuts at: 805 805
Size: 4274
Bsp1286I G_dGCh'C
Cuts at: 449 578 614 721 734 940 1212 1288 1899
Size: 129 36 107 13 206 272 76 611
Cuts at: 1899 1992 2066 2256 2318 3048 449
Size: 93 74 190 62 730 1675
Fragments arranged by size:
1675 730 611 272 206 190 129 107
93 76 74 62 36 13
BspEI T'CCGG_A
Cuts at: 726 3494 726
Size: 2768 1506
BspHI T'CATG_A
Cuts at: 422 3450 422
Size: 3028 1246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1001 1270 1324 1538 1840 2221 1001
Size: 269 54 214 302 381 3054
Fragments arranged by size:
3054 381 302 269 214 54
BsrBI CCG'CTC
Cuts at: 50 858 915 2566 2620 4104 4269 50
Size: 808 57 1651 54 1484 165 55
Fragments arranged by size:
1651 1484 808 165 57 55 54
BsrDI GCAATG_nn'
Cuts at: 2186 2186
Size: 4274
BsrFI r'CCGG_y
Cuts at: 112 135 430 648 896 2272 2453 2662 112
Size: 23 295 218 248 1376 181 209 1724
Fragments arranged by size:
1724 1376 295 248 218 209 181 23
BssHII G'CGCG_C
Cuts at: 200 1135 200
Size: 935 3339
BssSI C'ACGA_G
Cuts at: 2545 2903 2545
Size: 358 3916
BstAPI GCAn_nnn'nTGC
Cuts at: 1216 1651 1216
Size: 435 3839
BstBI TT'CG_AA
Cuts at: 2635 2635
Size: 4274
BstEII G'GTnAC_C
Cuts at: 138 1254 138
Size: 1116 3158
BstXI CCAn_nnnn'nTGG
Cuts at: 714 714
Size: 4274
BstYI r'GATC_y
Cuts at: 1 191 821 1031 1166 1299 1766 1789 2124
Size: 190 630 210 135 133 467 23 335
Cuts at: 2124 2370 3382 3468 3480 3826 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 630 467 449 346 335 246 210
190 135 133 86 23 12
BtgI C'CryG_G
Cuts at: 482 1250 482
Size: 768 3506
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2224 2405 2568 2224
Size: 181 163 3930
Fragments arranged by size:
3930 181 163
BtsI GCAGTG_nn'
Cuts at: 1517 1517
Size: 4274
DraI TTT'AAA
Cuts at: 1273 1273
Size: 4274
DrdI GACnn_nn'nnGTC
Cuts at: 235 1980 2838 235
Size: 1745 858 1671
Fragments arranged by size:
1745 1671 858
EaeI y'GGCC_r
Cuts at: 485 641 941 1859 2033 2424 2451 3796 485
Size: 156 300 918 174 391 27 1345 963
Fragments arranged by size:
1345 963 918 391 300 174 156 27
EagI C'GGCC_G
Cuts at: 941 1859 941
Size: 918 3356
EarI CTCTTCn'nnn_
Cuts at: 217 673 2297 2507 217
Size: 456 1624 210 1984
Fragments arranged by size:
1984 1624 456 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1210 2482 2792 2938 4084 1210
Size: 1272 310 146 1146 1400
Fragments arranged by size:
1400 1272 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1286 1286
Size: 4274
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 440 811 1032 2099 2531 3278 3546 440
Size: 371 221 1067 432 747 268 1168
Fragments arranged by size:
1168 1067 747 432 371 268 221
EcoO109I rG'GnC_Cy
Cuts at: 1413 1413
Size: 4274
EcoRI G'AATT_C
Cuts at: 1278 1278
Size: 4274
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 393 2293 393
Size: 1900 2374
FspI TGC'GCA
Cuts at: 997 2055 997
Size: 1058 3216
HaeII r_GCGC'y
Cuts at: 431 466 1158 1231 1708 1956 2978 3957 431
Size: 35 692 73 477 248 1022 979 748
Fragments arranged by size:
1022 979 748 692 477 248 73 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 553 565 585 597 622 654 703 735 2116
Size: 12 20 12 25 32 49 32 1381
Cuts at: 2116 2146 2148 2178 553
Size: 30 2 30 2649
Fragments arranged by size:
2649 1381 49 32 32 30 30 25
20 12 12 2
HincII GTy'rAC
Cuts at: 768 1313 768
Size: 545 3729
KasI G'GCGC_C
Cuts at: 1227 1704 1952 1227
Size: 477 248 3549
Fragments arranged by size:
3549 477 248
KpnI G_GTAC'C
Cuts at: 1294 1294
Size: 4274
MluI A'CGCG_T
Cuts at: 932 3707 932
Size: 2775 1499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 416 575 635 2945 3129 416
Size: 159 60 2310 184 1561
Fragments arranged by size:
2310 1561 184 159 60
MscI TGG'CCA
Cuts at: 2035 2035
Size: 4274
MslI CAynn'nnrTG
Cuts at: 2263 2390 2263
Size: 127 4147
MspA1I CmG'CkG
Cuts at: 1385 1699 2059 3072 3317 1385
Size: 314 360 1013 245 2342
Fragments arranged by size:
2342 1013 360 314 245
NaeI GCC'GGC
Cuts at: 432 650 2455 432
Size: 218 1805 2251
Fragments arranged by size:
2251 1805 218
NarI GG'CG_CC
Cuts at: 1228 1705 1953 1228
Size: 477 248 3549
Fragments arranged by size:
3549 477 248
NgoMIV G'CCGG_C
Cuts at: 430 648 2453 430
Size: 218 1805 2251
Fragments arranged by size:
2251 1805 218
NspI r_CATG'y
Cuts at: 1041 1327 1827 2734 1041
Size: 286 500 907 2581
Fragments arranged by size:
2581 907 500 286
PasI CC'CwG_GG
Cuts at: 597 597
Size: 4274
PciI A'CATG_T
Cuts at: 2730 2730
Size: 4274
PflMI CCAn_nnn'nTGG
Cuts at: 750 750
Size: 4274
PmeI GTTT'AAAC
Cuts at: 1273 1273
Size: 4274
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 4242
PshAI GACnn'nnGTC
Cuts at: 278 278
Size: 4274
PstI C_TGCA'G
Cuts at: 1321 2006 1321
Size: 685 3589
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4274
PvuII CAG'CTG
Cuts at: 1699 2059 1699
Size: 360 3914
RsrII CG'GwC_CG
Cuts at: 2469 2469
Size: 4274
SacI G_AGCT'C
Cuts at: 1288 1288
Size: 4274
SalI G'TCGA_C
Cuts at: 1311 1311
Size: 4274
SapI GCTCTTCn'nnn_
Cuts at: 2297 2507 2297
Size: 210 4064
SbfI CC_TGCA'GG
Cuts at: 1321 1321
Size: 4274
ScaI AGT'ACT
Cuts at: 3791 3791
Size: 4274
SfcI C'TryA_G
Cuts at: 33 257 1317 2002 2995 3186 33
Size: 224 1060 685 993 191 1121
Fragments arranged by size:
1121 1060 993 685 224 191
SfoI GGC'GCC
Cuts at: 1229 1706 1954 1229
Size: 477 248 3549
Fragments arranged by size:
3549 477 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4274
SmaI CCC'GGG
Cuts at: 609 830 1296 609
Size: 221 466 3587
Fragments arranged by size:
3587 466 221
SmlI C'TyrA_G
Cuts at: 865 1174 1429 2344 2836 3098 865
Size: 309 255 915 492 262 2041
Fragments arranged by size:
2041 915 492 309 262 255
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4274
SphI G_CATG'C
Cuts at: 1041 1327 1827 1041
Size: 286 500 3488
Fragments arranged by size:
3488 500 286
StyI C'CwwG_G
Cuts at: 1408 1762 1408
Size: 354 3920
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 268 1857 1990 2449 2658 268
Size: 1589 133 459 209 1884
Fragments arranged by size:
1884 1589 459 209 133
TatI w'GTAC_w
Cuts at: 3789 3789
Size: 4274
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3791 3823 3791
Size: 32 4242
Tth111I GACn'n_nGTC
Cuts at: 2071 2071
Size: 4274
XbaI T'CTAG_A
Cuts at: 1305 1305
Size: 4274
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 489 651 832 1769 489
Size: 162 181 937 2994
Fragments arranged by size:
2994 937 181 162
XmaI C'CCGG_G
Cuts at: 607 828 1294 607
Size: 221 466 3587
Fragments arranged by size:
3587 466 221
ZraI GAC'GTC
Cuts at: 479 479
Size: 4274
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI Bpu10I
BsiWI BsmBI BsrGI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PfoI PmlI
PpuMI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI