Restriction map for
pF1KB8984
(Circular) (Six-base) MAPSORT of: pF1KB8984.seq Check: 9466 from: 1 to: 4271
pF1KB8984 4271 bp
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1164 1164
Size: 4271
AccI GT'mk_AC
Cuts at: 1309 1309
Size: 4271
Acc65I G'GTAC_C
Cuts at: 1287 1287
Size: 4271
AclI AA'CG_TT
Cuts at: 4111 4111
Size: 4271
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2096 2528 3275 3543 2096
Size: 432 747 268 2824
Fragments arranged by size:
2824 747 432 268
AfeI AGC'GCT
Cuts at: 457 457
Size: 4271
AflIII A'CryG_T
Cuts at: 1623 2727 3704 1623
Size: 1104 977 2190
Fragments arranged by size:
2190 1104 977
AgeI A'CCGG_T
Cuts at: 2659 2659
Size: 4271
AleI CACnn'nnGTG
Cuts at: 136 136
Size: 4271
AlwNI CAG_nnn'CTG
Cuts at: 737 1576 3143 737
Size: 839 1567 1865
Fragments arranged by size:
1865 1567 839
ApaI G_GGCC'C
Cuts at: 506 639 1113 506
Size: 133 474 3664
Fragments arranged by size:
3664 474 133
ApaLI G'TGCA_C
Cuts at: 3041 3041
Size: 4271
ApoI r'AATT_y
Cuts at: 1275 3624 3880 1275
Size: 2349 256 1666
Fragments arranged by size:
2349 1666 256
AscI GG'CGCG_CC
Cuts at: 238 238
Size: 4271
AseI AT'TA_AT
Cuts at: 20 20
Size: 4271
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4271
AvaI C'yCGr_G
Cuts at: 362 1042 1291 362
Size: 680 249 3342
Fragments arranged by size:
3342 680 249
BamHI G'GATC_C
Cuts at: 1296 1296
Size: 4271
BanI G'GyrC_C
Cuts at: 295 649 1287 1701 1949 1984 295
Size: 354 638 414 248 35 2582
Fragments arranged by size:
2582 638 414 354 248 35
BanII G_rGCy'C
Cuts at: 148 313 506 639 775 1113 1184 1285 2315
Size: 165 193 133 136 338 71 101 1030
Cuts at: 2315 148
Size: 2104
Fragments arranged by size:
2104 1030 338 193 165 136 133 101
71
BbeI G_GCGC'C
Cuts at: 653 1705 1953 653
Size: 1052 248 2971
Fragments arranged by size:
2971 1052 248
BbsI GAAGACnn'nnnn_
Cuts at: 972 996 972
Size: 24 4247
BbvCI CC'TCA_GC
Cuts at: 730 730
Size: 4271
BciVI GTATCCnnnnn_n'
Cuts at: 2169 2930 4271 2169
Size: 761 1341 2169
Fragments arranged by size:
2169 1341 761
BclI T'GATC_A
Cuts at: 1791 1791
Size: 4271
BglI GCCn_nnn'nGGC
Cuts at: 520 770 520
Size: 250 4021
BglII A'GATC_T
Cuts at: 1786 3823 1786
Size: 2037 2234
BlpI GC'TnA_GC
Cuts at: 469 1383 469
Size: 914 3357
Bme1580I G_kGCm'C
Cuts at: 298 506 639 1113 1896 1989 3045 298
Size: 208 133 474 783 93 1056 1524
Fragments arranged by size:
1524 1056 783 474 208 133 93
BmrI ACTGGGnnnn_n'
Cuts at: 1483 1564 1899 4043 1483
Size: 81 335 2144 1711
Fragments arranged by size:
2144 1711 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2638 2638
Size: 4271
Bpu10I CC'TnA_GC
Cuts at: 220 307 730 220
Size: 87 423 3761
Fragments arranged by size:
3761 423 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 1447 2326 2818 3116 1000
Size: 447 879 492 298 2155
Fragments arranged by size:
2155 879 492 447 298
BsaI GGTCTCn'nnnn_
Cuts at: 3975 3975
Size: 4271
BsaAI yAC'GTr
Cuts at: 138 1624 138
Size: 1486 2785
BsaBI GATnn'nnATC
Cuts at: 6 1785 3822 6
Size: 1779 2037 455
Fragments arranged by size:
2037 1779 455
BsaHI Gr'CG_yC
Cuts at: 421 650 1702 1950 2652 4148 421
Size: 229 1052 248 702 1496 544
Fragments arranged by size:
1496 1052 702 544 248 229
BsaWI w'CCGG_w
Cuts at: 268 796 1460 1981 2659 2933 3080 3491 268
Size: 528 664 521 678 274 147 411 1048
Fragments arranged by size:
1048 678 664 528 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1110 1110
Size: 4271
BseYI C'CCAG_C
Cuts at: 291 351 573 632 638 1079 1696 2454 3031
Size: 60 222 59 6 441 617 758 577
Cuts at: 3031 3527 291
Size: 496 1035
Fragments arranged by size:
1035 758 617 577 496 441 222 60
59 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 141
Size: 4271
BsiEI CG_ry'CG
Cuts at: 104 235 317 790 1813 1859 3067 104
Size: 131 82 473 1023 46 1208 1308
Fragments arranged by size:
1308 1208 1023 473 131 82 46
BsiHKAI G_wGCw'C
Cuts at: 862 1285 2063 2253 3045 862
Size: 423 778 190 792 2088
Fragments arranged by size:
2088 792 778 423 190
Bsp1286I G_dGCh'C
Cuts at: 148 298 313 506 639 775 862 1113 1184
Size: 150 15 193 133 136 87 251 71
Cuts at: 1184 1285 1896 1989 2063 2253 2315 3045 148
Size: 101 611 93 74 190 62 730 1374
Fragments arranged by size:
1374 730 611 251 193 190 150 136
133 101 93 87 74 71 62 15
BspEI T'CCGG_A
Cuts at: 3491 3491
Size: 4271
BspHI T'CATG_A
Cuts at: 836 3447 836
Size: 2611 1660
BspMI ACCTGCnnnn'nnnn_
Cuts at: 296 1164 1321 1535 1837 2218 296
Size: 868 157 214 302 381 2349
Fragments arranged by size:
2349 868 381 302 214 157
BsrBI CCG'CTC
Cuts at: 50 2563 2617 4101 4266 50
Size: 2513 54 1484 165 55
Fragments arranged by size:
2513 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2183 2183
Size: 4271
BsrFI r'CCGG_y
Cuts at: 156 184 234 673 2269 2450 2659 156
Size: 28 50 439 1596 181 209 1768
Fragments arranged by size:
1768 1596 439 209 181 50 28
BssHII G'CGCG_C
Cuts at: 238 334 560 238
Size: 96 226 3949
Fragments arranged by size:
3949 226 96
BssSI C'ACGA_G
Cuts at: 2542 2900 2542
Size: 358 3913
BstAPI GCAn_nnn'nTGC
Cuts at: 614 1648 614
Size: 1034 3237
BstBI TT'CG_AA
Cuts at: 2632 2632
Size: 4271
BstEII G'GTnAC_C
Cuts at: 264 723 264
Size: 459 3812
BstXI CCAn_nnnn'nTGG
Cuts at: 450 450
Size: 4271
BstYI r'GATC_y
Cuts at: 1 1296 1763 1786 2121 2367 3379 3465 3477
Size: 1295 467 23 335 246 1012 86 12
Cuts at: 3477 3823 1
Size: 346 449
Fragments arranged by size:
1295 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 443 449 784 1053 1217 107
Size: 336 6 335 269 164 3161
Fragments arranged by size:
3161 336 335 269 164 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 398 2221 2402 2565 398
Size: 1823 181 163 2104
Fragments arranged by size:
2104 1823 181 163
BtsI GCAGTG_nn'
Cuts at: 921 1514 921
Size: 593 3678
DraI TTT'AAA
Cuts at: 1270 1270
Size: 4271
DrdI GACnn_nn'nnGTC
Cuts at: 976 1977 2835 976
Size: 1001 858 2412
Fragments arranged by size:
2412 1001 858
EaeI y'GGCC_r
Cuts at: 158 182 278 314 446 787 851 1856 2030
Size: 24 96 36 132 341 64 1005 174
Cuts at: 2030 2421 2448 3793 158
Size: 391 27 1345 636
Fragments arranged by size:
1345 1005 636 391 341 174 132 96
64 36 27 24
EagI C'GGCC_G
Cuts at: 314 787 1856 314
Size: 473 1069 2729
Fragments arranged by size:
2729 1069 473
EarI CTCTTCn'nnn_
Cuts at: 559 2294 2504 559
Size: 1735 210 2326
Fragments arranged by size:
2326 1735 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 2479 2789 2935 4081 127
Size: 2352 310 146 1146 317
Fragments arranged by size:
2352 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 1283 1283
Size: 4271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2096 2528 3275 3543 2096
Size: 432 747 268 2824
Fragments arranged by size:
2824 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1092 1092
Size: 4271
EcoO109I rG'GnC_Cy
Cuts at: 503 927 1101 1109 1110 1410 503
Size: 424 174 8 1 300 3364
Fragments arranged by size:
3364 424 300 174 8 1
EcoRI G'AATT_C
Cuts at: 1275 1275
Size: 4271
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1020 2290 1020
Size: 1270 3001
FseI GG_CCGG'CC
Cuts at: 160 160
Size: 4271
FspI TGC'GCA
Cuts at: 2052 2052
Size: 4271
HaeII r_GCGC'y
Cuts at: 459 469 526 653 765 1217 1705 1953 2975
Size: 10 57 127 112 452 488 248 1022
Cuts at: 2975 3954 459
Size: 979 776
Fragments arranged by size:
1022 979 776 488 452 248 127 112
57 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1004 1036 2113 2143 2145 2175 1004
Size: 32 1077 30 2 30 3100
Fragments arranged by size:
3100 1077 32 30 30 2
HincII GTy'rAC
Cuts at: 1310 1310
Size: 4271
KasI G'GCGC_C
Cuts at: 649 1701 1949 649
Size: 1052 248 2971
Fragments arranged by size:
2971 1052 248
KpnI G_GTAC'C
Cuts at: 1291 1291
Size: 4271
MluI A'CGCG_T
Cuts at: 3704 3704
Size: 4271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2942 3126 2942
Size: 184 4087
MscI TGG'CCA
Cuts at: 2032 2032
Size: 4271
MslI CAynn'nnrTG
Cuts at: 136 172 448 841 853 2260 2387 136
Size: 36 276 393 12 1407 127 2020
Fragments arranged by size:
2020 1407 393 276 127 36 12
MspA1I CmG'CkG
Cuts at: 495 509 611 881 1208 1382 1696 2056 3069
Size: 14 102 270 327 174 314 360 1013
Cuts at: 3069 3314 495
Size: 245 1452
Fragments arranged by size:
1452 1013 360 327 314 270 245 174
102 14
NaeI GCC'GGC
Cuts at: 158 675 2452 158
Size: 517 1777 1977
Fragments arranged by size:
1977 1777 517
NarI GG'CG_CC
Cuts at: 650 1702 1950 650
Size: 1052 248 2971
Fragments arranged by size:
2971 1052 248
NcoI C'CATG_G
Cuts at: 107 443 107
Size: 336 3935
NgoMIV G'CCGG_C
Cuts at: 156 673 2450 156
Size: 517 1777 1977
Fragments arranged by size:
1977 1777 517
NspI r_CATG'y
Cuts at: 630 747 1324 1824 2731 630
Size: 117 577 500 907 2170
Fragments arranged by size:
2170 907 577 500 117
PasI CC'CwG_GG
Cuts at: 210 974 210
Size: 764 3507
PciI A'CATG_T
Cuts at: 2727 2727
Size: 4271
PfoI T'CCnGG_A
Cuts at: 965 1090 965
Size: 125 4146
PmeI GTTT'AAAC
Cuts at: 1270 1270
Size: 4271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3410 3442 3410
Size: 32 4239
PpuMI rG'GwC_Cy
Cuts at: 927 1101 927
Size: 174 4097
PshAI GACnn'nnGTC
Cuts at: 844 844
Size: 4271
PspOMI G'GGCC_C
Cuts at: 502 635 1109 502
Size: 133 474 3664
Fragments arranged by size:
3664 474 133
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 118 150 118
Size: 32 4239
PstI C_TGCA'G
Cuts at: 1318 2003 1318
Size: 685 3586
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4271
PvuII CAG'CTG
Cuts at: 495 611 1696 2056 495
Size: 116 1085 360 2710
Fragments arranged by size:
2710 1085 360 116
RsrII CG'GwC_CG
Cuts at: 2466 2466
Size: 4271
SacI G_AGCT'C
Cuts at: 1285 1285
Size: 4271
SalI G'TCGA_C
Cuts at: 1308 1308
Size: 4271
SanDI GG'GwC_CC
Cuts at: 1101 1101
Size: 4271
SapI GCTCTTCn'nnn_
Cuts at: 2294 2504 2294
Size: 210 4061
SbfI CC_TGCA'GG
Cuts at: 1318 1318
Size: 4271
ScaI AGT'ACT
Cuts at: 598 3788 598
Size: 3190 1081
SfcI C'TryA_G
Cuts at: 33 1314 1999 2992 3183 33
Size: 1281 685 993 191 1121
Fragments arranged by size:
1281 1121 993 685 191
SfoI GGC'GCC
Cuts at: 651 1703 1951 651
Size: 1052 248 2971
Fragments arranged by size:
2971 1052 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4271
SmaI CCC'GGG
Cuts at: 1293 1293
Size: 4271
SmlI C'TyrA_G
Cuts at: 1015 1426 2341 2833 3095 1015
Size: 411 915 492 262 2191
Fragments arranged by size:
2191 915 492 411 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4271
SphI G_CATG'C
Cuts at: 630 1324 1824 630
Size: 694 500 3077
Fragments arranged by size:
3077 694 500
StyI C'CwwG_G
Cuts at: 107 443 1105 1405 1759 107
Size: 336 662 300 354 2619
Fragments arranged by size:
2619 662 354 336 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 711 904 1854 1987 2446 2655 711
Size: 193 950 133 459 209 2327
Fragments arranged by size:
2327 950 459 209 193 133
TatI w'GTAC_w
Cuts at: 596 3786 596
Size: 3190 1081
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3788 3820 3788
Size: 32 4239
Tth111I GACn'n_nGTC
Cuts at: 2068 2068
Size: 4271
XbaI T'CTAG_A
Cuts at: 1302 1302
Size: 4271
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 288 1766 288
Size: 1478 2793
XmaI C'CCGG_G
Cuts at: 1291 1291
Size: 4271
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PfoI PmeI PpiI PpuMI PshAI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AvrII BaeI BcgI
BfrBI BmgBI BmtI BpmI BsaXI BsiWI BsmI BsmBI
BsrGI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PmlI PsiI PspXI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI