Restriction map for
pF1KB8982
(Circular) (Six-base) MAPSORT of: pF1KB8982.seq Check: 7335 from: 1 to: 4247
pF1KB8982 4247 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1285 1285
Size: 4247
Acc65I G'GTAC_C
Cuts at: 1263 1263
Size: 4247
AclI AA'CG_TT
Cuts at: 4087 4087
Size: 4247
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2072 2504 3251 3519 2072
Size: 432 747 268 2800
Fragments arranged by size:
2800 747 432 268
AflIII A'CryG_T
Cuts at: 1599 2703 3680 1599
Size: 1104 977 2166
Fragments arranged by size:
2166 1104 977
AgeI A'CCGG_T
Cuts at: 2635 2635
Size: 4247
AleI CACnn'nnGTG
Cuts at: 1103 1103
Size: 4247
AlwNI CAG_nnn'CTG
Cuts at: 1552 3119 1552
Size: 1567 2680
ApaI G_GGCC'C
Cuts at: 311 311
Size: 4247
ApaLI G'TGCA_C
Cuts at: 3017 3017
Size: 4247
ApoI r'AATT_y
Cuts at: 738 1251 3600 3856 738
Size: 513 2349 256 1129
Fragments arranged by size:
2349 1129 513 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4247
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4247
AvaI C'yCGr_G
Cuts at: 184 678 1267 184
Size: 494 589 3164
Fragments arranged by size:
3164 589 494
BamHI G'GATC_C
Cuts at: 1272 1272
Size: 4247
BanI G'GyrC_C
Cuts at: 129 522 657 883 928 1231 1263 1677 1925
Size: 393 135 226 45 303 32 414 248
Cuts at: 1925 1960 129
Size: 35 2416
Fragments arranged by size:
2416 414 393 303 248 226 135 45
35 32
BanII G_rGCy'C
Cuts at: 115 182 311 988 1024 1031 1261 2291 115
Size: 67 129 677 36 7 230 1030 2071
Fragments arranged by size:
2071 1030 677 230 129 67 36 7
BbeI G_GCGC'C
Cuts at: 133 932 1681 1929 133
Size: 799 749 248 2451
Fragments arranged by size:
2451 799 749 248
BbsI GAAGACnn'nnnn_
Cuts at: 811 811
Size: 4247
BbvCI CC'TCA_GC
Cuts at: 239 1090 239
Size: 851 3396
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1110 1144 1221 1255 1110
Size: 34 77 34 4102
Fragments arranged by size:
4102 77 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2145 2906 4247 2145
Size: 761 1341 2145
Fragments arranged by size:
2145 1341 761
BclI T'GATC_A
Cuts at: 1767 1767
Size: 4247
BglI GCCn_nnn'nGGC
Cuts at: 793 793
Size: 4247
BglII A'GATC_T
Cuts at: 551 1762 3799 551
Size: 1211 2037 999
Fragments arranged by size:
2037 1211 999
BlpI GC'TnA_GC
Cuts at: 1144 1165 1359 1144
Size: 21 194 4032
Fragments arranged by size:
4032 194 21
Bme1580I G_kGCm'C
Cuts at: 233 311 525 662 886 1234 1872 1965 3021
Size: 78 214 137 224 348 638 93 1056
Cuts at: 3021 233
Size: 1459
Fragments arranged by size:
1459 1056 638 348 224 214 137 93
78
BmrI ACTGGGnnnn_n'
Cuts at: 150 1025 1125 1459 1540 1875 4019 150
Size: 875 100 334 81 335 2144 378
Fragments arranged by size:
2144 875 378 335 334 100 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2614 2614
Size: 4247
Bpu10I CC'TnA_GC
Cuts at: 239 1025 1090 239
Size: 786 65 3396
Fragments arranged by size:
3396 786 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1423 2302 2794 3092 1423
Size: 879 492 298 2578
Fragments arranged by size:
2578 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3951 3951
Size: 4247
BsaAI yAC'GTr
Cuts at: 564 1600 564
Size: 1036 3211
BsaBI GATnn'nnATC
Cuts at: 6 1761 3798 6
Size: 1755 2037 455
Fragments arranged by size:
2037 1755 455
BsaHI Gr'CG_yC
Cuts at: 130 929 1678 1926 2628 4124 130
Size: 799 749 248 702 1496 253
Fragments arranged by size:
1496 799 749 702 253 248
BsaWI w'CCGG_w
Cuts at: 1436 1957 2635 2909 3056 3467 1436
Size: 521 678 274 147 411 2216
Fragments arranged by size:
2216 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1057 1087 1145 1175 1057
Size: 30 58 30 4129
Fragments arranged by size:
4129 58 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 469 469
Size: 4247
BseYI C'CCAG_C
Cuts at: 163 232 397 1672 2430 3007 3503 163
Size: 69 165 1275 758 577 496 907
Fragments arranged by size:
1275 907 758 577 496 165 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 400
Size: 4247
BsiEI CG_ry'CG
Cuts at: 104 1789 1835 3043 104
Size: 1685 46 1208 1308
Fragments arranged by size:
1685 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 115 1166 1261 2039 2229 3021 115
Size: 1051 95 778 190 792 1341
Fragments arranged by size:
1341 1051 792 778 190 95
Bsp1286I G_dGCh'C
Cuts at: 115 182 233 311 525 662 886 988 1024
Size: 67 51 78 214 137 224 102 36
Cuts at: 1024 1031 1166 1234 1261 1872 1965 2039 2229
Size: 7 135 68 27 611 93 74 190
Cuts at: 2229 2291 3021 115
Size: 62 730 1341
Fragments arranged by size:
1341 730 611 224 214 190 137 135
102 93 78 74 68 67 62 51
36 27 7
BspEI T'CCGG_A
Cuts at: 3467 3467
Size: 4247
BspHI T'CATG_A
Cuts at: 3423 3423
Size: 4247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 131 341 1297 1511 1813 2194 131
Size: 210 956 214 302 381 2184
Fragments arranged by size:
2184 956 381 302 214 210
BsrBI CCG'CTC
Cuts at: 50 977 1080 2539 2593 4077 4242 50
Size: 927 103 1459 54 1484 165 55
Fragments arranged by size:
1484 1459 927 165 103 55 54
BsrDI GCAATG_nn'
Cuts at: 2159 2159
Size: 4247
BsrFI r'CCGG_y
Cuts at: 126 601 2245 2426 2635 126
Size: 475 1644 181 209 1738
Fragments arranged by size:
1738 1644 475 209 181
BssSI C'ACGA_G
Cuts at: 990 2518 2876 990
Size: 1528 358 2361
Fragments arranged by size:
2361 1528 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1624 1624
Size: 4247
BstBI TT'CG_AA
Cuts at: 2608 2608
Size: 4247
BstXI CCAn_nnnn'nTGG
Cuts at: 373 1105 373
Size: 732 3515
BstYI r'GATC_y
Cuts at: 1 551 1156 1272 1739 1762 2097 2343 3355
Size: 550 605 116 467 23 335 246 1012
Cuts at: 3355 3441 3453 3799 1
Size: 86 12 346 449
Fragments arranged by size:
1012 605 550 467 449 346 335 246
116 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 851 851
Size: 4247
BtgI C'CryG_G
Cuts at: 506 516 842 506
Size: 10 326 3911
Fragments arranged by size:
3911 326 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 486 2197 2378 2541 486
Size: 1711 181 163 2192
Fragments arranged by size:
2192 1711 181 163
BtsI GCAGTG_nn'
Cuts at: 1490 1490
Size: 4247
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1188 1223 1188
Size: 35 4212
DraI TTT'AAA
Cuts at: 1246 1246
Size: 4247
DrdI GACnn_nn'nnGTC
Cuts at: 1953 2811 1953
Size: 858 3389
EaeI y'GGCC_r
Cuts at: 171 475 509 566 1832 2006 2397 2424 3769
Size: 304 34 57 1266 174 391 27 1345
Cuts at: 3769 171
Size: 649
Fragments arranged by size:
1345 1266 649 391 304 174 57 34
27
EagI C'GGCC_G
Cuts at: 1832 1832
Size: 4247
EarI CTCTTCn'nnn_
Cuts at: 751 2270 2480 751
Size: 1519 210 2518
Fragments arranged by size:
2518 1519 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 610 2455 2765 2911 4057 610
Size: 1845 310 146 1146 800
Fragments arranged by size:
1845 1146 800 310 146
EcoICRI GAG'CTC
Cuts at: 113 1259 113
Size: 1146 3101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2072 2504 3251 3519 2072
Size: 432 747 268 2800
Fragments arranged by size:
2800 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 364 364
Size: 4247
EcoO109I rG'GnC_Cy
Cuts at: 1386 1386
Size: 4247
EcoRI G'AATT_C
Cuts at: 1251 1251
Size: 4247
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2266 2266
Size: 4247
FspI TGC'GCA
Cuts at: 2028 2028
Size: 4247
HaeII r_GCGC'y
Cuts at: 133 537 932 1681 1929 2951 3930 133
Size: 404 395 749 248 1022 979 450
Fragments arranged by size:
1022 979 749 450 404 395 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 760 792 856 888 1148 1180 2089 2119 2121
Size: 32 64 32 260 32 909 30 2
Cuts at: 2121 2151 760
Size: 30 2856
Fragments arranged by size:
2856 909 260 64 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1200 1286 1200
Size: 86 4161
KasI G'GCGC_C
Cuts at: 129 928 1677 1925 129
Size: 799 749 248 2451
Fragments arranged by size:
2451 799 749 248
KpnI G_GTAC'C
Cuts at: 1267 1267
Size: 4247
MluI A'CGCG_T
Cuts at: 3680 3680
Size: 4247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2918 3102 2918
Size: 184 4063
MscI TGG'CCA
Cuts at: 511 2008 511
Size: 1497 2750
MslI CAynn'nnrTG
Cuts at: 505 1103 1138 2236 2363 505
Size: 598 35 1098 127 2389
Fragments arranged by size:
2389 1098 598 127 35
MspA1I CmG'CkG
Cuts at: 243 390 1103 1358 1672 2032 3045 3290 243
Size: 147 713 255 314 360 1013 245 1200
Fragments arranged by size:
1200 1013 713 360 314 255 245 147
NaeI GCC'GGC
Cuts at: 128 603 2428 128
Size: 475 1825 1947
Fragments arranged by size:
1947 1825 475
NarI GG'CG_CC
Cuts at: 130 929 1678 1926 130
Size: 799 749 248 2451
Fragments arranged by size:
2451 799 749 248
NcoI C'CATG_G
Cuts at: 506 506
Size: 4247
NgoMIV G'CCGG_C
Cuts at: 126 601 2426 126
Size: 475 1825 1947
Fragments arranged by size:
1947 1825 475
NspI r_CATG'y
Cuts at: 1143 1300 1800 2707 1143
Size: 157 500 907 2683
Fragments arranged by size:
2683 907 500 157
PasI CC'CwG_GG
Cuts at: 1223 1223
Size: 4247
PciI A'CATG_T
Cuts at: 2703 2703
Size: 4247
PflMI CCAn_nnn'nTGG
Cuts at: 564 1002 564
Size: 438 3809
PmeI GTTT'AAAC
Cuts at: 1246 1246
Size: 4247
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 709 741 3386 3418 709
Size: 32 2645 32 1538
Fragments arranged by size:
2645 1538 32 32
PspOMI G'GGCC_C
Cuts at: 307 307
Size: 4247
PstI C_TGCA'G
Cuts at: 151 1294 1979 151
Size: 1143 685 2419
Fragments arranged by size:
2419 1143 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4247
PvuII CAG'CTG
Cuts at: 1672 2032 1672
Size: 360 3887
RsrII CG'GwC_CG
Cuts at: 2442 2442
Size: 4247
SacI G_AGCT'C
Cuts at: 115 1261 115
Size: 1146 3101
SalI G'TCGA_C
Cuts at: 1284 1284
Size: 4247
SapI GCTCTTCn'nnn_
Cuts at: 2270 2480 2270
Size: 210 4037
SbfI CC_TGCA'GG
Cuts at: 1294 1294
Size: 4247
ScaI AGT'ACT
Cuts at: 3764 3764
Size: 4247
SfcI C'TryA_G
Cuts at: 33 147 1290 1975 2968 3159 33
Size: 114 1143 685 993 191 1121
Fragments arranged by size:
1143 1121 993 685 191 114
SfoI GGC'GCC
Cuts at: 131 930 1679 1927 131
Size: 799 749 248 2451
Fragments arranged by size:
2451 799 749 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4247
SmaI CCC'GGG
Cuts at: 680 1269 680
Size: 589 3658
SmlI C'TyrA_G
Cuts at: 1402 2317 2809 3071 1402
Size: 915 492 262 2578
Fragments arranged by size:
2578 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4247
SphI G_CATG'C
Cuts at: 1300 1800 1300
Size: 500 3747
StuI AGG'CCT
Cuts at: 370 370
Size: 4247
StyI C'CwwG_G
Cuts at: 506 791 1001 1381 1735 506
Size: 285 210 380 354 3018
Fragments arranged by size:
3018 380 354 285 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 233 1830 1963 2422 2631 233
Size: 1597 133 459 209 1849
Fragments arranged by size:
1849 1597 459 209 133
TatI w'GTAC_w
Cuts at: 3762 3762
Size: 4247
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1145 1177 3764 3796 1145
Size: 32 2587 32 1596
Fragments arranged by size:
2587 1596 32 32
Tth111I GACn'n_nGTC
Cuts at: 2044 2044
Size: 4247
XbaI T'CTAG_A
Cuts at: 1278 1278
Size: 4247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1742 1742
Size: 4247
XmaI C'CCGG_G
Cuts at: 678 1267 678
Size: 589 3658
XmnI GAAnn'nnTTC
Cuts at: 211 724 211
Size: 513 3734
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BfrBI BmgBI BmtI BpmI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I ClaI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PfoI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI