Restriction map for
pF1KB8980
(Circular) (Six-base) MAPSORT of: pF1KB8980.seq Check: 3642 from: 1 to: 4241
pF1KB8980 4241 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1279 1279
Size: 4241
Acc65I G'GTAC_C
Cuts at: 1257 1257
Size: 4241
AclI AA'CG_TT
Cuts at: 4081 4081
Size: 4241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2066 2498 3245 3513 2066
Size: 432 747 268 2794
Fragments arranged by size:
2794 747 432 268
AfeI AGC'GCT
Cuts at: 641 641
Size: 4241
AflIII A'CryG_T
Cuts at: 1593 2697 3674 1593
Size: 1104 977 2160
Fragments arranged by size:
2160 1104 977
AgeI A'CCGG_T
Cuts at: 2629 2629
Size: 4241
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 376 376
Size: 4241
AlwNI CAG_nnn'CTG
Cuts at: 1227 1546 3113 1227
Size: 319 1567 2355
Fragments arranged by size:
2355 1567 319
ApaI G_GGCC'C
Cuts at: 834 834
Size: 4241
ApaLI G'TGCA_C
Cuts at: 3011 3011
Size: 4241
ApoI r'AATT_y
Cuts at: 118 589 1245 3594 3850 118
Size: 471 656 2349 256 509
Fragments arranged by size:
2349 656 509 471 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4241
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
AvaI C'yCGr_G
Cuts at: 385 1261 385
Size: 876 3365
BamHI G'GATC_C
Cuts at: 1266 1266
Size: 4241
BanI G'GyrC_C
Cuts at: 360 370 539 1257 1671 1919 1954 360
Size: 10 169 718 414 248 35 2647
Fragments arranged by size:
2647 718 414 248 169 35 10
BanII G_rGCy'C
Cuts at: 337 834 1255 2285 337
Size: 497 421 1030 2293
Fragments arranged by size:
2293 1030 497 421
BbeI G_GCGC'C
Cuts at: 374 543 1675 1923 374
Size: 169 1132 248 2692
Fragments arranged by size:
2692 1132 248 169
BbsI GAAGACnn'nnnn_
Cuts at: 802 802
Size: 4241
BbvCI CC'TCA_GC
Cuts at: 487 487
Size: 4241
BciVI GTATCCnnnnn_n'
Cuts at: 2139 2900 4241 2139
Size: 761 1341 2139
Fragments arranged by size:
2139 1341 761
BclI T'GATC_A
Cuts at: 1761 1761
Size: 4241
BglI GCCn_nnn'nGGC
Cuts at: 341 341
Size: 4241
BglII A'GATC_T
Cuts at: 1756 3793 1756
Size: 2037 2204
BlpI GC'TnA_GC
Cuts at: 1353 1353
Size: 4241
Bme1580I G_kGCm'C
Cuts at: 365 834 1866 1959 3015 365
Size: 469 1032 93 1056 1591
Fragments arranged by size:
1591 1056 1032 469 93
BmrI ACTGGGnnnn_n'
Cuts at: 708 919 1127 1453 1534 1869 4013 708
Size: 211 208 326 81 335 2144 936
Fragments arranged by size:
2144 936 335 326 211 208 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1083 2608 1083
Size: 1525 2716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 859 859
Size: 4241
Bpu10I CC'TnA_GC
Cuts at: 487 817 487
Size: 330 3911
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 1078 1417 2296 2788 3086 778
Size: 300 339 879 492 298 1933
Fragments arranged by size:
1933 879 492 339 300 298
BsaI GGTCTCn'nnnn_
Cuts at: 3945 3945
Size: 4241
BsaAI yAC'GTr
Cuts at: 1594 1594
Size: 4241
BsaBI GATnn'nnATC
Cuts at: 6 1755 3792 6
Size: 1749 2037 455
Fragments arranged by size:
2037 1749 455
BsaHI Gr'CG_yC
Cuts at: 371 540 1672 1920 2622 4118 371
Size: 169 1132 248 702 1496 494
Fragments arranged by size:
1496 1132 702 494 248 169
BsaWI w'CCGG_w
Cuts at: 686 1430 1951 2629 2903 3050 3461 686
Size: 744 521 678 274 147 411 1466
Fragments arranged by size:
1466 744 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 245 793 245
Size: 548 3693
BseYI C'CCAG_C
Cuts at: 277 1666 2424 3001 3497 277
Size: 1389 758 577 496 1021
Fragments arranged by size:
1389 1021 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 321 443 1783 1829 3037 104
Size: 217 122 1340 46 1208 1308
Fragments arranged by size:
1340 1308 1208 217 122 46
BsiHKAI G_wGCw'C
Cuts at: 1255 2033 2223 3015 1255
Size: 778 190 792 2481
Fragments arranged by size:
2481 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 828 828
Size: 4241
Bsp1286I G_dGCh'C
Cuts at: 337 365 834 1255 1866 1959 2033 2223 2285
Size: 28 469 421 611 93 74 190 62
Cuts at: 2285 3015 337
Size: 730 1563
Fragments arranged by size:
1563 730 611 469 421 190 93 74
62 28
BspEI T'CCGG_A
Cuts at: 3461 3461
Size: 4241
BspHI T'CATG_A
Cuts at: 3417 3417
Size: 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1291 1505 1807 2188 1291
Size: 214 302 381 3344
Fragments arranged by size:
3344 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2533 2587 4071 4236 50
Size: 2483 54 1484 165 55
Fragments arranged by size:
2483 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 951 2153 951
Size: 1202 3039
BsrFI r'CCGG_y
Cuts at: 367 438 472 2239 2420 2629 367
Size: 71 34 1767 181 209 1979
Fragments arranged by size:
1979 1767 209 181 71 34
BssSI C'ACGA_G
Cuts at: 2512 2870 2512
Size: 358 3883
BstAPI GCAn_nnn'nTGC
Cuts at: 1618 1618
Size: 4241
BstBI TT'CG_AA
Cuts at: 2602 2602
Size: 4241
BstXI CCAn_nnnn'nTGG
Cuts at: 313 1021 313
Size: 708 3533
BstYI r'GATC_y
Cuts at: 1 1266 1733 1756 2091 2337 3349 3435 3447
Size: 1265 467 23 335 246 1012 86 12
Cuts at: 3447 3793 1
Size: 346 449
Fragments arranged by size:
1265 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1049 1049
Size: 4241
BtgI C'CryG_G
Cuts at: 306 338 348 306
Size: 32 10 4199
Fragments arranged by size:
4199 32 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2191 2372 2535 2191
Size: 181 163 3897
Fragments arranged by size:
3897 181 163
BtsI GCAGTG_nn'
Cuts at: 1484 1484
Size: 4241
DraI TTT'AAA
Cuts at: 1240 1240
Size: 4241
DrdI GACnn_nn'nnGTC
Cuts at: 1947 2805 1947
Size: 858 3383
EaeI y'GGCC_r
Cuts at: 318 416 440 1826 2000 2391 2418 3763 318
Size: 98 24 1386 174 391 27 1345 796
Fragments arranged by size:
1386 1345 796 391 174 98 27 24
EagI C'GGCC_G
Cuts at: 318 440 1826 318
Size: 122 1386 2733
Fragments arranged by size:
2733 1386 122
EarI CTCTTCn'nnn_
Cuts at: 785 2264 2474 785
Size: 1479 210 2552
Fragments arranged by size:
2552 1479 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2449 2759 2905 4051 2449
Size: 310 146 1146 2639
Fragments arranged by size:
2639 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1253 1253
Size: 4241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 859 2066 2498 3245 3513 859
Size: 1207 432 747 268 1587
Fragments arranged by size:
1587 1207 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 830 831 1053 1062 1380 830
Size: 1 222 9 318 3691
Fragments arranged by size:
3691 318 222 9 1
EcoRI G'AATT_C
Cuts at: 1245 1245
Size: 4241
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 819 2260 819
Size: 1441 2800
FspI TGC'GCA
Cuts at: 2022 2022
Size: 4241
HaeII r_GCGC'y
Cuts at: 314 374 543 643 1675 1923 2945 3924 314
Size: 60 169 100 1032 248 1022 979 631
Fragments arranged by size:
1032 1022 979 631 248 169 100 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 240 272 2083 2113 2115 2145 240
Size: 32 1811 30 2 30 2336
Fragments arranged by size:
2336 1811 32 30 30 2
HincII GTy'rAC
Cuts at: 1004 1280 1004
Size: 276 3965
KasI G'GCGC_C
Cuts at: 370 539 1671 1919 370
Size: 169 1132 248 2692
Fragments arranged by size:
2692 1132 248 169
KpnI G_GTAC'C
Cuts at: 1261 1261
Size: 4241
MluI A'CGCG_T
Cuts at: 3674 3674
Size: 4241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2912 3096 2912
Size: 184 4057
MscI TGG'CCA
Cuts at: 2002 2002
Size: 4241
MslI CAynn'nnrTG
Cuts at: 2230 2357 2230
Size: 127 4114
MspA1I CmG'CkG
Cuts at: 340 350 524 1174 1352 1666 2026 3039 3284
Size: 10 174 650 178 314 360 1013 245
Cuts at: 3284 340
Size: 1297
Fragments arranged by size:
1297 1013 650 360 314 245 178 174
10
NaeI GCC'GGC
Cuts at: 369 440 2422 369
Size: 71 1982 2188
Fragments arranged by size:
2188 1982 71
NarI GG'CG_CC
Cuts at: 371 540 1672 1920 371
Size: 169 1132 248 2692
Fragments arranged by size:
2692 1132 248 169
NgoMIV G'CCGG_C
Cuts at: 367 438 2420 367
Size: 71 1982 2188
Fragments arranged by size:
2188 1982 71
NotI GC'GGCC_GC
Cuts at: 318 318
Size: 4241
NspI r_CATG'y
Cuts at: 1294 1794 2701 1294
Size: 500 907 2834
Fragments arranged by size:
2834 907 500
PciI A'CATG_T
Cuts at: 2697 2697
Size: 4241
PmeI GTTT'AAAC
Cuts at: 1240 1240
Size: 4241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 3380
Size: 32 4209
PpuMI rG'GwC_Cy
Cuts at: 1062 1062
Size: 4241
PspOMI G'GGCC_C
Cuts at: 830 830
Size: 4241
PstI C_TGCA'G
Cuts at: 380 1112 1288 1973 380
Size: 732 176 685 2648
Fragments arranged by size:
2648 732 685 176
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4241
PvuII CAG'CTG
Cuts at: 1174 1666 2026 1174
Size: 492 360 3389
Fragments arranged by size:
3389 492 360
RsrII CG'GwC_CG
Cuts at: 2436 2436
Size: 4241
SacI G_AGCT'C
Cuts at: 1255 1255
Size: 4241
SacII CC_GC'GG
Cuts at: 341 351 341
Size: 10 4231
SalI G'TCGA_C
Cuts at: 1278 1278
Size: 4241
SapI GCTCTTCn'nnn_
Cuts at: 2264 2474 2264
Size: 210 4031
SbfI CC_TGCA'GG
Cuts at: 1288 1288
Size: 4241
ScaI AGT'ACT
Cuts at: 3758 3758
Size: 4241
SfcI C'TryA_G
Cuts at: 33 376 1108 1284 1969 2962 3153 33
Size: 343 732 176 685 993 191 1121
Fragments arranged by size:
1121 993 732 685 343 191 176
SfoI GGC'GCC
Cuts at: 372 541 1673 1921 372
Size: 169 1132 248 2692
Fragments arranged by size:
2692 1132 248 169
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
SgrAI Cr'CCGG_yG
Cuts at: 367 367
Size: 4241
SmaI CCC'GGG
Cuts at: 1263 1263
Size: 4241
SmlI C'TyrA_G
Cuts at: 757 1057 1396 2311 2803 3065 757
Size: 300 339 915 492 262 1933
Fragments arranged by size:
1933 915 492 339 300 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4241
SphI G_CATG'C
Cuts at: 1294 1794 1294
Size: 500 3741
StyI C'CwwG_G
Cuts at: 1375 1729 1375
Size: 354 3887
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 996 1146 1824 1957 2416 2625 996
Size: 150 678 133 459 209 2612
Fragments arranged by size:
2612 678 459 209 150 133
TatI w'GTAC_w
Cuts at: 3756 3756
Size: 4241
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 484 516 3758 3790 484
Size: 32 3242 32 935
Fragments arranged by size:
3242 935 32 32
Tth111I GACn'n_nGTC
Cuts at: 2038 2038
Size: 4241
XbaI T'CTAG_A
Cuts at: 573 1272 573
Size: 699 3542
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1736 1736
Size: 4241
XmaI C'CCGG_G
Cuts at: 1261 1261
Size: 4241
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NotI NspI PciI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsaXI BsgI BsiWI
BsmI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspXI PsrI SanDI SexAI SfiI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI