Restriction map for
pF1KB8978
(Circular) (Six-base) MAPSORT of: pF1KB8978.seq Check: 8962 from: 1 to: 4235
pF1KB8978 4235 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 960 960
Size: 4235
AccI GT'mk_AC
Cuts at: 1273 1273
Size: 4235
Acc65I G'GTAC_C
Cuts at: 1251 1251
Size: 4235
AclI AA'CG_TT
Cuts at: 4075 4075
Size: 4235
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 1195 1220 2060 2492 3239 3507 346
Size: 849 25 840 432 747 268 1074
Fragments arranged by size:
1074 849 840 747 432 268 25
AflIII A'CryG_T
Cuts at: 1587 2691 3668 1587
Size: 1104 977 2154
Fragments arranged by size:
2154 1104 977
AgeI A'CCGG_T
Cuts at: 2623 2623
Size: 4235
AleI CACnn'nnGTG
Cuts at: 970 970
Size: 4235
AlwNI CAG_nnn'CTG
Cuts at: 1540 3107 1540
Size: 1567 2668
ApaI G_GGCC'C
Cuts at: 234 234
Size: 4235
ApaLI G'TGCA_C
Cuts at: 3005 3005
Size: 4235
ApoI r'AATT_y
Cuts at: 208 916 1239 3588 3844 208
Size: 708 323 2349 256 599
Fragments arranged by size:
2349 708 599 323 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4235
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4235
AvaI C'yCGr_G
Cuts at: 774 1255 774
Size: 481 3754
BamHI G'GATC_C
Cuts at: 1260 1260
Size: 4235
BanI G'GyrC_C
Cuts at: 1251 1665 1913 1948 1251
Size: 414 248 35 3538
Fragments arranged by size:
3538 414 248 35
BanII G_rGCy'C
Cuts at: 234 405 1197 1249 2279 234
Size: 171 792 52 1030 2190
Fragments arranged by size:
2190 1030 792 171 52
BbeI G_GCGC'C
Cuts at: 1669 1917 1669
Size: 248 3987
BbsI GAAGACnn'nnnn_
Cuts at: 1201 1201
Size: 4235
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4201
BciVI GTATCCnnnnn_n'
Cuts at: 2133 2894 4235 2133
Size: 761 1341 2133
Fragments arranged by size:
2133 1341 761
BclI T'GATC_A
Cuts at: 593 1755 593
Size: 1162 3073
BglII A'GATC_T
Cuts at: 1750 3787 1750
Size: 2037 2198
BlpI GC'TnA_GC
Cuts at: 1347 1347
Size: 4235
Bme1580I G_kGCm'C
Cuts at: 234 1860 1953 3009 234
Size: 1626 93 1056 1460
Fragments arranged by size:
1626 1460 1056 93
BmgBI CAC'GTC
Cuts at: 216 216
Size: 4235
BmrI ACTGGGnnnn_n'
Cuts at: 1447 1528 1863 4007 1447
Size: 81 335 2144 1675
Fragments arranged by size:
2144 1675 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2602 2602
Size: 4235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1411 2290 2782 3080 1411
Size: 879 492 298 2566
Fragments arranged by size:
2566 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1113 3939 1113
Size: 2826 1409
BsaAI yAC'GTr
Cuts at: 1588 1588
Size: 4235
BsaBI GATnn'nnATC
Cuts at: 6 1749 3786 6
Size: 1743 2037 455
Fragments arranged by size:
2037 1743 455
BsaHI Gr'CG_yC
Cuts at: 1666 1914 2616 4112 1666
Size: 248 702 1496 1789
Fragments arranged by size:
1789 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1424 1945 2623 2897 3044 3455 1424
Size: 521 678 274 147 411 2204
Fragments arranged by size:
2204 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1139 1169 1139
Size: 30 4205
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1147 1147
Size: 4235
BseYI C'CCAG_C
Cuts at: 1660 2418 2995 3491 1660
Size: 758 577 496 2404
Fragments arranged by size:
2404 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1777 1823 3031 104
Size: 1673 46 1208 1308
Fragments arranged by size:
1673 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 198 1249 2027 2217 3009 198
Size: 1051 778 190 792 1424
Fragments arranged by size:
1424 1051 792 778 190
BsmI GAATG_Cn'
Cuts at: 441 441
Size: 4235
Bsp1286I G_dGCh'C
Cuts at: 198 234 405 1197 1249 1860 1953 2027 2217
Size: 36 171 792 52 611 93 74 190
Cuts at: 2217 2279 3009 198
Size: 62 730 1424
Fragments arranged by size:
1424 792 730 611 190 171 93 74
62 52 36
BspEI T'CCGG_A
Cuts at: 3455 3455
Size: 4235
BspHI T'CATG_A
Cuts at: 851 3411 851
Size: 2560 1675
BspMI ACCTGCnnnn'nnnn_
Cuts at: 356 960 1285 1499 1801 2182 356
Size: 604 325 214 302 381 2409
Fragments arranged by size:
2409 604 381 325 302 214
BsrBI CCG'CTC
Cuts at: 50 155 249 2527 2581 4065 4230 50
Size: 105 94 2278 54 1484 165 55
Fragments arranged by size:
2278 1484 165 105 94 55 54
BsrDI GCAATG_nn'
Cuts at: 446 2147 446
Size: 1701 2534
BsrFI r'CCGG_y
Cuts at: 2233 2414 2623 2233
Size: 181 209 3845
Fragments arranged by size:
3845 209 181
BssSI C'ACGA_G
Cuts at: 692 2506 2864 692
Size: 1814 358 2063
Fragments arranged by size:
2063 1814 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1612 1612
Size: 4235
BstBI TT'CG_AA
Cuts at: 2596 2596
Size: 4235
BstEII G'GTnAC_C
Cuts at: 567 567
Size: 4235
BstXI CCAn_nnnn'nTGG
Cuts at: 698 954 698
Size: 256 3979
BstYI r'GATC_y
Cuts at: 1 1260 1727 1750 2085 2331 3343 3429 3441
Size: 1259 467 23 335 246 1012 86 12
Cuts at: 3441 3787 1
Size: 346 449
Fragments arranged by size:
1259 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2185 2366 2529 2185
Size: 181 163 3891
Fragments arranged by size:
3891 181 163
BtsI GCAGTG_nn'
Cuts at: 836 996 1478 836
Size: 160 482 3593
Fragments arranged by size:
3593 482 160
ClaI AT'CG_AT
Cuts at: 515 515
Size: 4235
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 159 194 159
Size: 35 4200
DraI TTT'AAA
Cuts at: 554 1234 554
Size: 680 3555
DrdI GACnn_nn'nnGTC
Cuts at: 1941 2799 1941
Size: 858 3377
EaeI y'GGCC_r
Cuts at: 359 1820 1994 2385 2412 3757 359
Size: 1461 174 391 27 1345 837
Fragments arranged by size:
1461 1345 837 391 174 27
EagI C'GGCC_G
Cuts at: 1820 1820
Size: 4235
EarI CTCTTCn'nnn_
Cuts at: 811 874 2258 2468 811
Size: 63 1384 210 2578
Fragments arranged by size:
2578 1384 210 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 500 2443 2753 2899 4045 500
Size: 1943 310 146 1146 690
Fragments arranged by size:
1943 1146 690 310 146
EcoICRI GAG'CTC
Cuts at: 1247 1247
Size: 4235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 1195 1220 2060 2492 3239 3507 346
Size: 849 25 840 432 747 268 1074
Fragments arranged by size:
1074 849 840 747 432 268 25
EcoO109I rG'GnC_Cy
Cuts at: 230 1374 230
Size: 1144 3091
EcoRI G'AATT_C
Cuts at: 916 1239 916
Size: 323 3912
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1225 2254 1225
Size: 1029 3206
FspI TGC'GCA
Cuts at: 2016 2016
Size: 4235
HaeII r_GCGC'y
Cuts at: 1669 1917 2939 3918 1669
Size: 248 1022 979 1986
Fragments arranged by size:
1986 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 966 998 2077 2107 2109 2139 966
Size: 32 1079 30 2 30 3062
Fragments arranged by size:
3062 1079 32 30 30 2
HincII GTy'rAC
Cuts at: 952 1274 952
Size: 322 3913
KasI G'GCGC_C
Cuts at: 1665 1913 1665
Size: 248 3987
KpnI G_GTAC'C
Cuts at: 1255 1255
Size: 4235
MfeI C'AATT_G
Cuts at: 1184 1184
Size: 4235
MluI A'CGCG_T
Cuts at: 3668 3668
Size: 4235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2906 3090 2906
Size: 184 4051
MscI TGG'CCA
Cuts at: 361 1996 361
Size: 1635 2600
MslI CAynn'nnrTG
Cuts at: 696 718 850 970 2224 2351 696
Size: 22 132 120 1254 127 2580
Fragments arranged by size:
2580 1254 132 127 120 22
MspA1I CmG'CkG
Cuts at: 750 1346 1660 2020 3033 3278 750
Size: 596 314 360 1013 245 1707
Fragments arranged by size:
1707 1013 596 360 314 245
NaeI GCC'GGC
Cuts at: 2416 2416
Size: 4235
NarI GG'CG_CC
Cuts at: 1666 1914 1666
Size: 248 3987
NgoMIV G'CCGG_C
Cuts at: 2414 2414
Size: 4235
NspI r_CATG'y
Cuts at: 291 1288 1788 2695 291
Size: 997 500 907 1831
Fragments arranged by size:
1831 997 907 500
PciI A'CATG_T
Cuts at: 2691 2691
Size: 4235
PflMI CCAn_nnn'nTGG
Cuts at: 1102 1102
Size: 4235
PfoI T'CCnGG_A
Cuts at: 911 1110 911
Size: 199 4036
PmeI GTTT'AAAC
Cuts at: 1234 1234
Size: 4235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3374 3406 3374
Size: 32 4203
PspOMI G'GGCC_C
Cuts at: 230 230
Size: 4235
PstI C_TGCA'G
Cuts at: 1282 1967 1282
Size: 685 3550
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4235
PvuII CAG'CTG
Cuts at: 1660 2020 1660
Size: 360 3875
RsrII CG'GwC_CG
Cuts at: 2430 2430
Size: 4235
SacI G_AGCT'C
Cuts at: 1249 1249
Size: 4235
SalI G'TCGA_C
Cuts at: 1272 1272
Size: 4235
SapI GCTCTTCn'nnn_
Cuts at: 2258 2468 2258
Size: 210 4025
SbfI CC_TGCA'GG
Cuts at: 1282 1282
Size: 4235
ScaI AGT'ACT
Cuts at: 3752 3752
Size: 4235
SexAI A'CCwGG_T
Cuts at: 598 598
Size: 4235
SfcI C'TryA_G
Cuts at: 33 1071 1278 1963 2956 3147 33
Size: 1038 207 685 993 191 1121
Fragments arranged by size:
1121 1038 993 685 207 191
SfoI GGC'GCC
Cuts at: 1667 1915 1667
Size: 248 3987
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4235
SmaI CCC'GGG
Cuts at: 1257 1257
Size: 4235
SmlI C'TyrA_G
Cuts at: 1390 2305 2797 3059 1390
Size: 915 492 262 2566
Fragments arranged by size:
2566 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4235
SphI G_CATG'C
Cuts at: 1288 1788 1288
Size: 500 3735
SspI AAT'ATT
Cuts at: 888 888
Size: 4235
StyI C'CwwG_G
Cuts at: 1369 1723 1369
Size: 354 3881
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1818 1951 2410 2619 1818
Size: 133 459 209 3434
Fragments arranged by size:
3434 459 209 133
TatI w'GTAC_w
Cuts at: 3750 3750
Size: 4235
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1118 1139 1150 1171 3752 3784 1118
Size: 21 11 21 2581 32 1569
Fragments arranged by size:
2581 1569 32 21 21 11
Tth111I GACn'n_nGTC
Cuts at: 2032 2032
Size: 4235
XbaI T'CTAG_A
Cuts at: 1266 1266
Size: 4235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1730 1730
Size: 4235
XmaI C'CCGG_G
Cuts at: 1255 1255
Size: 4235
XmnI GAAnn'nnTTC
Cuts at: 706 907 706
Size: 201 4034
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BglI BmtI BpmI Bpu10I BsgI
BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I BtgI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI