Restriction map for
pF1KB8976
(Circular) (Six-base) MAPSORT of: pF1KB8976.seq Check: 4785 from: 1 to: 4229
pF1KB8976 4229 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1267 1267
Size: 4229
Acc65I G'GTAC_C
Cuts at: 1245 1245
Size: 4229
AclI AA'CG_TT
Cuts at: 4069 4069
Size: 4229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 988 2054 2486 3233 3501 988
Size: 1066 432 747 268 1716
Fragments arranged by size:
1716 1066 747 432 268
AflIII A'CryG_T
Cuts at: 1581 2685 3662 1581
Size: 1104 977 2148
Fragments arranged by size:
2148 1104 977
AgeI A'CCGG_T
Cuts at: 2617 2617
Size: 4229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 535 535
Size: 4229
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 142 174 142
Size: 32 4197
AlwNI CAG_nnn'CTG
Cuts at: 1534 3101 1534
Size: 1567 2662
ApaI G_GGCC'C
Cuts at: 239 239
Size: 4229
ApaLI G'TGCA_C
Cuts at: 832 2999 832
Size: 2167 2062
ApoI r'AATT_y
Cuts at: 1233 3582 3838 1233
Size: 2349 256 1624
Fragments arranged by size:
2349 1624 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4229
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4229
AvaI C'yCGr_G
Cuts at: 139 1249 139
Size: 1110 3119
BamHI G'GATC_C
Cuts at: 1254 1254
Size: 4229
BanI G'GyrC_C
Cuts at: 341 857 1245 1659 1907 1942 341
Size: 516 388 414 248 35 2628
Fragments arranged by size:
2628 516 414 388 248 35
BanII G_rGCy'C
Cuts at: 239 442 1243 2273 239
Size: 203 801 1030 2195
Fragments arranged by size:
2195 1030 801 203
BbeI G_GCGC'C
Cuts at: 861 1663 1911 861
Size: 802 248 3179
Fragments arranged by size:
3179 802 248
BbsI GAAGACnn'nnnn_
Cuts at: 402 402
Size: 4229
BbvCI CC'TCA_GC
Cuts at: 863 863
Size: 4229
BciVI GTATCCnnnnn_n'
Cuts at: 2127 2888 4229 2127
Size: 761 1341 2127
Fragments arranged by size:
2127 1341 761
BclI T'GATC_A
Cuts at: 1749 1749
Size: 4229
BglII A'GATC_T
Cuts at: 1744 3781 1744
Size: 2037 2192
BlpI GC'TnA_GC
Cuts at: 1341 1341
Size: 4229
Bme1580I G_kGCm'C
Cuts at: 239 272 836 1854 1947 3003 239
Size: 33 564 1018 93 1056 1465
Fragments arranged by size:
1465 1056 1018 564 93 33
BmrI ACTGGGnnnn_n'
Cuts at: 1441 1522 1857 4001 1441
Size: 81 335 2144 1669
Fragments arranged by size:
2144 1669 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2596 2596
Size: 4229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 355 169
Size: 186 4043
Bpu10I CC'TnA_GC
Cuts at: 863 863
Size: 4229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1405 2284 2776 3074 1405
Size: 879 492 298 2560
Fragments arranged by size:
2560 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 361 3933 361
Size: 3572 657
BsaAI yAC'GTr
Cuts at: 822 1582 822
Size: 760 3469
BsaBI GATnn'nnATC
Cuts at: 6 1743 3780 6
Size: 1737 2037 455
Fragments arranged by size:
2037 1737 455
BsaHI Gr'CG_yC
Cuts at: 858 1660 1908 2610 4106 858
Size: 802 248 702 1496 981
Fragments arranged by size:
1496 981 802 702 248
BsaWI w'CCGG_w
Cuts at: 1418 1939 2617 2891 3038 3449 1418
Size: 521 678 274 147 411 2198
Fragments arranged by size:
2198 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 142 172 181 211 682 712 142
Size: 30 9 30 471 30 3659
Fragments arranged by size:
3659 471 30 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 586 586
Size: 4229
BseYI C'CCAG_C
Cuts at: 449 851 1125 1654 2412 2989 3485 449
Size: 402 274 529 758 577 496 1193
Fragments arranged by size:
1193 758 577 529 496 402 274
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 475
Size: 4229
BsiEI CG_ry'CG
Cuts at: 104 1771 1817 3025 104
Size: 1667 46 1208 1308
Fragments arranged by size:
1667 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 836 1243 2021 2211 3003 836
Size: 407 778 190 792 2062
Fragments arranged by size:
2062 792 778 407 190
BsmI GAATG_Cn'
Cuts at: 1206 1206
Size: 4229
Bsp1286I G_dGCh'C
Cuts at: 239 272 442 836 1243 1854 1947 2021 2211
Size: 33 170 394 407 611 93 74 190
Cuts at: 2211 2273 3003 239
Size: 62 730 1465
Fragments arranged by size:
1465 730 611 407 394 190 170 93
74 62 33
BspEI T'CCGG_A
Cuts at: 3449 3449
Size: 4229
BspHI T'CATG_A
Cuts at: 3405 3405
Size: 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 994 1279 1493 1795 2176 994
Size: 285 214 302 381 3047
Fragments arranged by size:
3047 381 302 285 214
BsrBI CCG'CTC
Cuts at: 50 339 2521 2575 4059 4224 50
Size: 289 2182 54 1484 165 55
Fragments arranged by size:
2182 1484 289 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2141 2141
Size: 4229
BsrFI r'CCGG_y
Cuts at: 344 1081 1135 2227 2408 2617 344
Size: 737 54 1092 181 209 1956
Fragments arranged by size:
1956 1092 737 209 181 54
BssSI C'ACGA_G
Cuts at: 2500 2858 2500
Size: 358 3871
BstAPI GCAn_nnn'nTGC
Cuts at: 840 1606 840
Size: 766 3463
BstBI TT'CG_AA
Cuts at: 2590 2590
Size: 4229
BstEII G'GTnAC_C
Cuts at: 923 923
Size: 4229
BstXI CCAn_nnnn'nTGG
Cuts at: 630 630
Size: 4229
BstYI r'GATC_y
Cuts at: 1 1254 1721 1744 2079 2325 3337 3423 3435
Size: 1253 467 23 335 246 1012 86 12
Cuts at: 3435 3781 1
Size: 346 449
Fragments arranged by size:
1253 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 272 362 389 428 539 974 1010 272
Size: 90 27 39 111 435 36 3491
Fragments arranged by size:
3491 435 111 90 39 36 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 519 2179 2360 2523 519
Size: 1660 181 163 2225
Fragments arranged by size:
2225 1660 181 163
BtsI GCAGTG_nn'
Cuts at: 1047 1104 1472 1047
Size: 57 368 3804
Fragments arranged by size:
3804 368 57
DraI TTT'AAA
Cuts at: 1228 1228
Size: 4229
DraIII CAC_nnn'GTG
Cuts at: 1025 1025
Size: 4229
DrdI GACnn_nn'nnGTC
Cuts at: 1935 2793 1935
Size: 858 3371
EaeI y'GGCC_r
Cuts at: 386 392 431 554 887 1013 1814 1988 2379
Size: 6 39 123 333 126 801 174 391
Cuts at: 2379 2406 3751 386
Size: 27 1345 864
Fragments arranged by size:
1345 864 801 391 333 174 126 123
39 27 6
EagI C'GGCC_G
Cuts at: 1814 1814
Size: 4229
EarI CTCTTCn'nnn_
Cuts at: 670 1165 2252 2462 670
Size: 495 1087 210 2437
Fragments arranged by size:
2437 1087 495 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2437 2747 2893 4039 2437
Size: 310 146 1146 2627
Fragments arranged by size:
2627 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1241 1241
Size: 4229
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 355 988 2054 2486 3233 3501 169
Size: 186 633 1066 432 747 268 897
Fragments arranged by size:
1066 897 747 633 432 268 186
EcoO109I rG'GnC_Cy
Cuts at: 153 235 236 453 461 1368 153
Size: 82 1 217 8 907 3014
Fragments arranged by size:
3014 907 217 82 8 1
EcoRI G'AATT_C
Cuts at: 1233 1233
Size: 4229
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2248 2248
Size: 4229
FspI TGC'GCA
Cuts at: 2010 2010
Size: 4229
HaeII r_GCGC'y
Cuts at: 861 1663 1911 2933 3912 861
Size: 802 248 1022 979 1178
Fragments arranged by size:
1178 1022 979 802 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2071 2101 2103 2133 2071
Size: 30 2 30 4167
Fragments arranged by size:
4167 30 30 2
HincII GTy'rAC
Cuts at: 501 1268 501
Size: 767 3462
KasI G'GCGC_C
Cuts at: 857 1659 1907 857
Size: 802 248 3179
Fragments arranged by size:
3179 802 248
KpnI G_GTAC'C
Cuts at: 1249 1249
Size: 4229
MluI A'CGCG_T
Cuts at: 3662 3662
Size: 4229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2900 3084 2900
Size: 184 4045
MscI TGG'CCA
Cuts at: 556 889 1015 1990 556
Size: 333 126 975 2795
Fragments arranged by size:
2795 975 333 126
MslI CAynn'nnrTG
Cuts at: 538 802 895 1021 2218 2345 538
Size: 264 93 126 1197 127 2422
Fragments arranged by size:
2422 1197 264 127 126 93
MspA1I CmG'CkG
Cuts at: 855 867 1340 1654 2014 3027 3272 855
Size: 12 473 314 360 1013 245 1812
Fragments arranged by size:
1812 1013 473 360 314 245 12
NaeI GCC'GGC
Cuts at: 1083 2410 1083
Size: 1327 2902
NarI GG'CG_CC
Cuts at: 858 1660 1908 858
Size: 802 248 3179
Fragments arranged by size:
3179 802 248
NgoMIV G'CCGG_C
Cuts at: 1081 2408 1081
Size: 1327 2902
NspI r_CATG'y
Cuts at: 748 1282 1782 2689 748
Size: 534 500 907 2288
Fragments arranged by size:
2288 907 534 500
PciI A'CATG_T
Cuts at: 2685 2685
Size: 4229
PfoI T'CCnGG_A
Cuts at: 332 947 332
Size: 615 3614
PmeI GTTT'AAAC
Cuts at: 1228 1228
Size: 4229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 142 174 3368 3400 142
Size: 32 3194 32 971
Fragments arranged by size:
3194 971 32 32
PpuMI rG'GwC_Cy
Cuts at: 153 453 461 153
Size: 300 8 3921
Fragments arranged by size:
3921 300 8
PspOMI G'GGCC_C
Cuts at: 235 235
Size: 4229
PstI C_TGCA'G
Cuts at: 514 1276 1961 514
Size: 762 685 2782
Fragments arranged by size:
2782 762 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4229
PvuII CAG'CTG
Cuts at: 1654 2014 1654
Size: 360 3869
RsrII CG'GwC_CG
Cuts at: 2424 2424
Size: 4229
SacI G_AGCT'C
Cuts at: 1243 1243
Size: 4229
SalI G'TCGA_C
Cuts at: 1266 1266
Size: 4229
SapI GCTCTTCn'nnn_
Cuts at: 1165 2252 2462 1165
Size: 1087 210 2932
Fragments arranged by size:
2932 1087 210
SbfI CC_TGCA'GG
Cuts at: 1276 1276
Size: 4229
ScaI AGT'ACT
Cuts at: 3746 3746
Size: 4229
SfcI C'TryA_G
Cuts at: 33 279 510 1272 1957 2950 3141 33
Size: 246 231 762 685 993 191 1121
Fragments arranged by size:
1121 993 762 685 246 231 191
SfoI GGC'GCC
Cuts at: 859 1661 1909 859
Size: 802 248 3179
Fragments arranged by size:
3179 802 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4229
SgrAI Cr'CCGG_yG
Cuts at: 1081 1081
Size: 4229
SmaI CCC'GGG
Cuts at: 1251 1251
Size: 4229
SmlI C'TyrA_G
Cuts at: 139 1384 2299 2791 3053 139
Size: 1245 915 492 262 1315
Fragments arranged by size:
1315 1245 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4229
SphI G_CATG'C
Cuts at: 748 1282 1782 748
Size: 534 500 3195
Fragments arranged by size:
3195 534 500
StyI C'CwwG_G
Cuts at: 681 1363 1717 681
Size: 682 354 3193
Fragments arranged by size:
3193 682 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1812 1945 2404 2613 1812
Size: 133 459 209 3428
Fragments arranged by size:
3428 459 209 133
TatI w'GTAC_w
Cuts at: 3744 3744
Size: 4229
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 682 714 3746 3778 682
Size: 32 3032 32 1133
Fragments arranged by size:
3032 1133 32 32
Tth111I GACn'n_nGTC
Cuts at: 2026 2026
Size: 4229
XbaI T'CTAG_A
Cuts at: 1260 1260
Size: 4229
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 613 630 804 1724 613
Size: 17 174 920 3118
Fragments arranged by size:
3118 920 174 17
XhoI C'TCGA_G
Cuts at: 139 139
Size: 4229
XmaI C'CCGG_G
Cuts at: 1249 1249
Size: 4229
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI AvrII
BaeI BcgI BfrBI BglI BmgBI BmtI BsiWI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SnaBI
SrfI SspI StuI SwaI XmnI ZraI