Restriction map for
pF1KB8974
(Circular) (Six-base) MAPSORT of: pF1KB8974.seq Check: 94 from: 1 to: 4220
pF1KB8974 4220 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 323 1258 323
Size: 935 3285
Acc65I G'GTAC_C
Cuts at: 1236 1236
Size: 4220
AclI AA'CG_TT
Cuts at: 4060 4060
Size: 4220
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 757 778 1123 2045 2477 3224 3492 757
Size: 21 345 922 432 747 268 1485
Fragments arranged by size:
1485 922 747 432 345 268 21
AflIII A'CryG_T
Cuts at: 359 1572 2676 3653 359
Size: 1213 1104 977 926
Fragments arranged by size:
1213 1104 977 926
AgeI A'CCGG_T
Cuts at: 2608 2608
Size: 4220
AleI CACnn'nnGTG
Cuts at: 340 340
Size: 4220
AlwNI CAG_nnn'CTG
Cuts at: 1525 3092 1525
Size: 1567 2653
ApaLI G'TGCA_C
Cuts at: 2990 2990
Size: 4220
ApoI r'AATT_y
Cuts at: 372 1224 3573 3829 372
Size: 852 2349 256 763
Fragments arranged by size:
2349 852 763 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4220
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4220
AvaI C'yCGr_G
Cuts at: 1240 1240
Size: 4220
BamHI G'GATC_C
Cuts at: 1245 1245
Size: 4220
BanI G'GyrC_C
Cuts at: 292 1236 1650 1898 1933 292
Size: 944 414 248 35 2579
Fragments arranged by size:
2579 944 414 248 35
BanII G_rGCy'C
Cuts at: 1234 2264 1234
Size: 1030 3190
BbeI G_GCGC'C
Cuts at: 1654 1902 1654
Size: 248 3972
BbsI GAAGACnn'nnnn_
Cuts at: 191 352 839 191
Size: 161 487 3572
Fragments arranged by size:
3572 487 161
BciVI GTATCCnnnnn_n'
Cuts at: 2118 2879 4220 2118
Size: 761 1341 2118
Fragments arranged by size:
2118 1341 761
BclI T'GATC_A
Cuts at: 515 1740 515
Size: 1225 2995
BglII A'GATC_T
Cuts at: 1735 3772 1735
Size: 2037 2183
BlpI GC'TnA_GC
Cuts at: 1332 1332
Size: 4220
Bme1580I G_kGCm'C
Cuts at: 1845 1938 2994 1845
Size: 93 1056 3071
Fragments arranged by size:
3071 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1125 1432 1513 1848 3992 1125
Size: 307 81 335 2144 1353
Fragments arranged by size:
2144 1353 335 307 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1212 2587 1212
Size: 1375 2845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1207 1207
Size: 4220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1396 2275 2767 3065 1396
Size: 879 492 298 2551
Fragments arranged by size:
2551 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3924 3924
Size: 4220
BsaAI yAC'GTr
Cuts at: 1573 1573
Size: 4220
BsaBI GATnn'nnATC
Cuts at: 6 1734 3771 6
Size: 1728 2037 455
Fragments arranged by size:
2037 1728 455
BsaHI Gr'CG_yC
Cuts at: 947 1651 1899 2601 4097 947
Size: 704 248 702 1496 1070
Fragments arranged by size:
1496 1070 704 702 248
BsaWI w'CCGG_w
Cuts at: 1409 1930 2608 2882 3029 3440 1409
Size: 521 678 274 147 411 2189
Fragments arranged by size:
2189 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 382 382
Size: 4220
BseYI C'CCAG_C
Cuts at: 1645 2403 2980 3476 1645
Size: 758 577 496 2389
Fragments arranged by size:
2389 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 684 955 318
Size: 366 271 3583
Fragments arranged by size:
3583 366 271
BsiEI CG_ry'CG
Cuts at: 104 1762 1808 3016 104
Size: 1658 46 1208 1308
Fragments arranged by size:
1658 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 570 730 1206 1234 2012 2202 2994 570
Size: 160 476 28 778 190 792 1796
Fragments arranged by size:
1796 792 778 476 190 160 28
Bsp1286I G_dGCh'C
Cuts at: 570 730 1206 1234 1845 1938 2012 2202 2264
Size: 160 476 28 611 93 74 190 62
Cuts at: 2264 2994 570
Size: 730 1796
Fragments arranged by size:
1796 730 611 476 190 160 93 74
62 28
BspEI T'CCGG_A
Cuts at: 3440 3440
Size: 4220
BspHI T'CATG_A
Cuts at: 3396 3396
Size: 4220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1270 1484 1786 2167 1270
Size: 214 302 381 3323
Fragments arranged by size:
3323 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2512 2566 4050 4215 50
Size: 2462 54 1484 165 55
Fragments arranged by size:
2462 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 422 542 548 689 2132 422
Size: 120 6 141 1443 2510
Fragments arranged by size:
2510 1443 141 120 6
BsrFI r'CCGG_y
Cuts at: 2218 2399 2608 2218
Size: 181 209 3830
Fragments arranged by size:
3830 209 181
BsrGI T'GTAC_A
Cuts at: 482 596 482
Size: 114 4106
BssSI C'ACGA_G
Cuts at: 277 1090 2491 2849 277
Size: 813 1401 358 1648
Fragments arranged by size:
1648 1401 813 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1597 1597
Size: 4220
BstBI TT'CG_AA
Cuts at: 2581 2581
Size: 4220
BstEII G'GTnAC_C
Cuts at: 1008 1008
Size: 4220
BstYI r'GATC_y
Cuts at: 1 1086 1245 1712 1735 2070 2316 3328 3414
Size: 1085 159 467 23 335 246 1012 86
Cuts at: 3414 3426 3772 1
Size: 12 346 449
Fragments arranged by size:
1085 1012 467 449 346 335 246 159
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1197 1197
Size: 4220
BtgI C'CryG_G
Cuts at: 179 704 179
Size: 525 3695
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 734 1005 2170 2351 2514 734
Size: 271 1165 181 163 2440
Fragments arranged by size:
2440 1165 271 181 163
BtsI GCAGTG_nn'
Cuts at: 1463 1463
Size: 4220
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 972 1007 972
Size: 35 4185
DraI TTT'AAA
Cuts at: 1219 1219
Size: 4220
DrdI GACnn_nn'nnGTC
Cuts at: 1926 2784 1926
Size: 858 3362
EaeI y'GGCC_r
Cuts at: 1805 1979 2370 2397 3742 1805
Size: 174 391 27 1345 2283
Fragments arranged by size:
2283 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1805 1805
Size: 4220
EarI CTCTTCn'nnn_
Cuts at: 413 2243 2453 413
Size: 1830 210 2180
Fragments arranged by size:
2180 1830 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1069 2428 2738 2884 4030 1069
Size: 1359 310 146 1146 1259
Fragments arranged by size:
1359 1259 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1232 1232
Size: 4220
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 757 778 1123 1207 2045 2477 3224 3492 757
Size: 21 345 84 838 432 747 268 1485
Fragments arranged by size:
1485 838 747 432 345 268 84 21
EcoO109I rG'GnC_Cy
Cuts at: 1359 1359
Size: 4220
EcoRI G'AATT_C
Cuts at: 1224 1224
Size: 4220
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 510 2239 510
Size: 1729 2491
FspI TGC'GCA
Cuts at: 2001 2001
Size: 4220
HaeII r_GCGC'y
Cuts at: 1654 1902 2924 3903 1654
Size: 248 1022 979 1971
Fragments arranged by size:
1971 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 145 177 2062 2092 2094 2124 145
Size: 32 1885 30 2 30 2241
Fragments arranged by size:
2241 1885 32 30 30 2
HincII GTy'rAC
Cuts at: 195 474 1259 195
Size: 279 785 3156
Fragments arranged by size:
3156 785 279
HindIII A'AGCT_T
Cuts at: 1161 1161
Size: 4220
HpaI GTT'AAC
Cuts at: 195 195
Size: 4220
KasI G'GCGC_C
Cuts at: 1650 1898 1650
Size: 248 3972
KpnI G_GTAC'C
Cuts at: 1240 1240
Size: 4220
MfeI C'AATT_G
Cuts at: 380 380
Size: 4220
MluI A'CGCG_T
Cuts at: 3653 3653
Size: 4220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 992 2891 3075 992
Size: 1899 184 2137
Fragments arranged by size:
2137 1899 184
MscI TGG'CCA
Cuts at: 1981 1981
Size: 4220
MslI CAynn'nnrTG
Cuts at: 340 529 2209 2336 340
Size: 189 1680 127 2224
Fragments arranged by size:
2224 1680 189 127
MspA1I CmG'CkG
Cuts at: 503 637 745 1331 1645 2005 3018 3263 503
Size: 134 108 586 314 360 1013 245 1460
Fragments arranged by size:
1460 1013 586 360 314 245 134 108
NaeI GCC'GGC
Cuts at: 2401 2401
Size: 4220
NarI GG'CG_CC
Cuts at: 1651 1899 1651
Size: 248 3972
NcoI C'CATG_G
Cuts at: 179 704 179
Size: 525 3695
NgoMIV G'CCGG_C
Cuts at: 2399 2399
Size: 4220
NspI r_CATG'y
Cuts at: 363 483 1273 1773 2680 363
Size: 120 790 500 907 1903
Fragments arranged by size:
1903 907 790 500 120
PciI A'CATG_T
Cuts at: 359 2676 359
Size: 2317 1903
PmeI GTTT'AAAC
Cuts at: 1219 1219
Size: 4220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3359 3391 3359
Size: 32 4188
PstI C_TGCA'G
Cuts at: 1267 1952 1267
Size: 685 3535
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4220
PvuII CAG'CTG
Cuts at: 503 637 745 1645 2005 503
Size: 134 108 900 360 2718
Fragments arranged by size:
2718 900 360 134 108
RsrII CG'GwC_CG
Cuts at: 2415 2415
Size: 4220
SacI G_AGCT'C
Cuts at: 1234 1234
Size: 4220
SalI G'TCGA_C
Cuts at: 1257 1257
Size: 4220
SapI GCTCTTCn'nnn_
Cuts at: 2243 2453 2243
Size: 210 4010
SbfI CC_TGCA'GG
Cuts at: 1267 1267
Size: 4220
ScaI AGT'ACT
Cuts at: 3737 3737
Size: 4220
SfcI C'TryA_G
Cuts at: 33 498 1263 1948 2941 3132 33
Size: 465 765 685 993 191 1121
Fragments arranged by size:
1121 993 765 685 465 191
SfoI GGC'GCC
Cuts at: 1652 1900 1652
Size: 248 3972
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4220
SmaI CCC'GGG
Cuts at: 1242 1242
Size: 4220
SmlI C'TyrA_G
Cuts at: 1375 2290 2782 3044 1375
Size: 915 492 262 2551
Fragments arranged by size:
2551 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4220
SphI G_CATG'C
Cuts at: 1273 1773 1273
Size: 500 3720
StyI C'CwwG_G
Cuts at: 179 296 704 1354 1708 179
Size: 117 408 650 354 2691
Fragments arranged by size:
2691 650 408 354 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 129 280 370 1027 1057 1803 1936 2395 2604
Size: 151 90 657 30 746 133 459 209
Cuts at: 2604 129
Size: 1745
Fragments arranged by size:
1745 746 657 459 209 151 133 90
30
TatI w'GTAC_w
Cuts at: 269 482 596 3735 269
Size: 213 114 3139 754
Fragments arranged by size:
3139 754 213 114
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3737 3769 3737
Size: 32 4188
Tth111I GACn'n_nGTC
Cuts at: 2017 2017
Size: 4220
XbaI T'CTAG_A
Cuts at: 1251 1251
Size: 4220
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 177 269 627 1715 177
Size: 92 358 1088 2682
Fragments arranged by size:
2682 1088 358 92
XmaI C'CCGG_G
Cuts at: 1240 1240
Size: 4220
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BssHII BstXI
BstZ17I ClaI DraIII EcoNI EcoRV FseI FspAI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI