Restriction map for
pF1KB8970
(Circular) (Six-base) MAPSORT of: pF1KB8970.seq Check: 3280 from: 1 to: 4190
pF1KB8970 4190 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1228 1228
Size: 4190
Acc65I G'GTAC_C
Cuts at: 1206 1206
Size: 4190
AclI AA'CG_TT
Cuts at: 4030 4030
Size: 4190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 1028 2015 2447 3194 3462 171
Size: 857 987 432 747 268 899
Fragments arranged by size:
987 899 857 747 432 268
AflIII A'CryG_T
Cuts at: 1542 2646 3623 1542
Size: 1104 977 2109
Fragments arranged by size:
2109 1104 977
AgeI A'CCGG_T
Cuts at: 2578 2578
Size: 4190
AleI CACnn'nnGTG
Cuts at: 145 492 145
Size: 347 3843
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 510 510
Size: 4190
AlwNI CAG_nnn'CTG
Cuts at: 833 879 1495 3062 833
Size: 46 616 1567 1961
Fragments arranged by size:
1961 1567 616 46
ApaLI G'TGCA_C
Cuts at: 2960 2960
Size: 4190
ApoI r'AATT_y
Cuts at: 1194 3543 3799 1194
Size: 2349 256 1585
Fragments arranged by size:
2349 1585 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4190
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
AvaI C'yCGr_G
Cuts at: 237 1210 237
Size: 973 3217
AvrII C'CTAG_G
Cuts at: 507 507
Size: 4190
BamHI G'GATC_C
Cuts at: 1215 1215
Size: 4190
BanI G'GyrC_C
Cuts at: 512 646 766 809 1206 1620 1868 1903 512
Size: 134 120 43 397 414 248 35 2799
Fragments arranged by size:
2799 414 397 248 134 120 43 35
BanII G_rGCy'C
Cuts at: 458 685 1204 2234 458
Size: 227 519 1030 2414
Fragments arranged by size:
2414 1030 519 227
BbeI G_GCGC'C
Cuts at: 770 1624 1872 770
Size: 854 248 3088
Fragments arranged by size:
3088 854 248
BbsI GAAGACnn'nnnn_
Cuts at: 279 1025 279
Size: 746 3444
BbvCI CC'TCA_GC
Cuts at: 872 872
Size: 4190
BciVI GTATCCnnnnn_n'
Cuts at: 2088 2849 4190 2088
Size: 761 1341 2088
Fragments arranged by size:
2088 1341 761
BclI T'GATC_A
Cuts at: 1710 1710
Size: 4190
BglI GCCn_nnn'nGGC
Cuts at: 203 203
Size: 4190
BglII A'GATC_T
Cuts at: 1087 1705 3742 1087
Size: 618 2037 1535
Fragments arranged by size:
2037 1535 618
BlpI GC'TnA_GC
Cuts at: 1302 1302
Size: 4190
Bme1580I G_kGCm'C
Cuts at: 515 1815 1908 2964 515
Size: 1300 93 1056 1741
Fragments arranged by size:
1741 1300 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1402 1483 1818 3962 1402
Size: 81 335 2144 1630
Fragments arranged by size:
2144 1630 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 699 2557 699
Size: 1858 2332
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 702 901 1076 511
Size: 191 199 175 3625
Fragments arranged by size:
3625 199 191 175
Bpu10I CC'TnA_GC
Cuts at: 343 872 343
Size: 529 3661
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1366 2245 2737 3035 1366
Size: 879 492 298 2521
Fragments arranged by size:
2521 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3894 3894
Size: 4190
BsaAI yAC'GTr
Cuts at: 1543 1543
Size: 4190
BsaBI GATnn'nnATC
Cuts at: 6 1041 1075 1704 3741 6
Size: 1035 34 629 2037 455
Fragments arranged by size:
2037 1035 629 455 34
BsaHI Gr'CG_yC
Cuts at: 261 767 1621 1869 2571 4067 261
Size: 506 854 248 702 1496 384
Fragments arranged by size:
1496 854 702 506 384 248
BsaWI w'CCGG_w
Cuts at: 377 1379 1900 2578 2852 2999 3410 377
Size: 1002 521 678 274 147 411 1157
Fragments arranged by size:
1157 1002 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 458 488 802 832 458
Size: 30 314 30 3816
Fragments arranged by size:
3816 314 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 450 691 898 450
Size: 241 207 3742
Fragments arranged by size:
3742 241 207
BseYI C'CCAG_C
Cuts at: 243 327 696 1615 2373 2950 3446 243
Size: 84 369 919 758 577 496 987
Fragments arranged by size:
987 919 758 577 496 369 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 652 807 1173 652
Size: 155 366 3669
Fragments arranged by size:
3669 366 155
BsiEI CG_ry'CG
Cuts at: 104 1732 1778 2986 104
Size: 1628 46 1208 1308
Fragments arranged by size:
1628 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 185 256 713 926 1204 1982 2172 2964 185
Size: 71 457 213 278 778 190 792 1411
Fragments arranged by size:
1411 792 778 457 278 213 190 71
Bsp1286I G_dGCh'C
Cuts at: 185 256 458 515 685 713 926 1204 1815
Size: 71 202 57 170 28 213 278 611
Cuts at: 1815 1908 1982 2172 2234 2964 185
Size: 93 74 190 62 730 1411
Fragments arranged by size:
1411 730 611 278 213 202 190 170
93 74 71 62 57 28
BspEI T'CCGG_A
Cuts at: 3410 3410
Size: 4190
BspHI T'CATG_A
Cuts at: 3366 3366
Size: 4190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 218 1240 1454 1756 2137 218
Size: 1022 214 302 381 2271
Fragments arranged by size:
2271 1022 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2482 2536 4020 4185 50
Size: 2432 54 1484 165 55
Fragments arranged by size:
2432 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2102 2102
Size: 4190
BsrFI r'CCGG_y
Cuts at: 193 794 1148 2188 2369 2578 193
Size: 601 354 1040 181 209 1805
Fragments arranged by size:
1805 1040 601 354 209 181
BssHII G'CGCG_C
Cuts at: 232 599 846 232
Size: 367 247 3576
Fragments arranged by size:
3576 367 247
BssSI C'ACGA_G
Cuts at: 673 2461 2819 673
Size: 1788 358 2044
Fragments arranged by size:
2044 1788 358
BstAPI GCAn_nnn'nTGC
Cuts at: 653 1567 653
Size: 914 3276
BstBI TT'CG_AA
Cuts at: 2551 2551
Size: 4190
BstEII G'GTnAC_C
Cuts at: 315 315
Size: 4190
BstXI CCAn_nnnn'nTGG
Cuts at: 147 489 726 1170 147
Size: 342 237 444 3167
Fragments arranged by size:
3167 444 342 237
BstYI r'GATC_y
Cuts at: 1 1087 1215 1682 1705 2040 2286 3298 3384
Size: 1086 128 467 23 335 246 1012 86
Cuts at: 3384 3396 3742 1
Size: 12 346 449
Fragments arranged by size:
1086 1012 467 449 346 335 246 128
86 23 12
BtgI C'CryG_G
Cuts at: 482 482
Size: 4190
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 802 2140 2321 2484 802
Size: 1338 181 163 2508
Fragments arranged by size:
2508 1338 181 163
BtsI GCAGTG_nn'
Cuts at: 265 466 1433 265
Size: 201 967 3022
Fragments arranged by size:
3022 967 201
DraI TTT'AAA
Cuts at: 1189 1189
Size: 4190
DraIII CAC_nnn'GTG
Cuts at: 653 935 653
Size: 282 3908
DrdI GACnn_nn'nnGTC
Cuts at: 1896 2754 1896
Size: 858 3332
EaeI y'GGCC_r
Cuts at: 479 1775 1949 2340 2367 3712 479
Size: 1296 174 391 27 1345 957
Fragments arranged by size:
1345 1296 957 391 174 27
EagI C'GGCC_G
Cuts at: 1775 1775
Size: 4190
EarI CTCTTCn'nnn_
Cuts at: 190 931 2213 2423 190
Size: 741 1282 210 1957
Fragments arranged by size:
1957 1282 741 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2398 2708 2854 4000 2398
Size: 310 146 1146 2588
Fragments arranged by size:
2588 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1202 1202
Size: 4190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 511 702 901 1028 1076 2015 2447 3194
Size: 340 191 199 127 48 939 432 747
Cuts at: 3194 3462 171
Size: 268 899
Fragments arranged by size:
939 899 747 432 340 268 199 191
127 48
EcoNI CCTnn'n_nnAGG
Cuts at: 825 825
Size: 4190
EcoO109I rG'GnC_Cy
Cuts at: 1001 1329 1001
Size: 328 3862
EcoRI G'AATT_C
Cuts at: 1194 1194
Size: 4190
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 4190
FspI TGC'GCA
Cuts at: 775 1971 775
Size: 1196 2994
HaeII r_GCGC'y
Cuts at: 581 733 770 913 1624 1872 2894 3873 581
Size: 152 37 143 711 248 1022 979 898
Fragments arranged by size:
1022 979 898 711 248 152 143 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 521 553 2032 2062 2064 2094 521
Size: 32 1479 30 2 30 2617
Fragments arranged by size:
2617 1479 32 30 30 2
HincII GTy'rAC
Cuts at: 1229 1229
Size: 4190
KasI G'GCGC_C
Cuts at: 766 1620 1868 766
Size: 854 248 3088
Fragments arranged by size:
3088 854 248
KpnI G_GTAC'C
Cuts at: 1210 1210
Size: 4190
MfeI C'AATT_G
Cuts at: 1055 1055
Size: 4190
MluI A'CGCG_T
Cuts at: 3623 3623
Size: 4190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2861 3045 2861
Size: 184 4006
MscI TGG'CCA
Cuts at: 481 1951 481
Size: 1470 2720
MslI CAynn'nnrTG
Cuts at: 145 298 492 1168 2179 2306 145
Size: 153 194 676 1011 127 2029
Fragments arranged by size:
2029 1011 676 194 153 127
MspA1I CmG'CkG
Cuts at: 128 276 807 1301 1615 1975 2988 3233 128
Size: 148 531 494 314 360 1013 245 1085
Fragments arranged by size:
1085 1013 531 494 360 314 245 148
NaeI GCC'GGC
Cuts at: 2371 2371
Size: 4190
NarI GG'CG_CC
Cuts at: 767 1621 1869 767
Size: 854 248 3088
Fragments arranged by size:
3088 854 248
NcoI C'CATG_G
Cuts at: 482 482
Size: 4190
NgoMIV G'CCGG_C
Cuts at: 2369 2369
Size: 4190
NspI r_CATG'y
Cuts at: 297 1243 1743 2650 297
Size: 946 500 907 1837
Fragments arranged by size:
1837 946 907 500
PasI CC'CwG_GG
Cuts at: 219 219
Size: 4190
PciI A'CATG_T
Cuts at: 2646 2646
Size: 4190
PmeI GTTT'AAAC
Cuts at: 1189 1189
Size: 4190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 127 159 3329 3361 127
Size: 32 3170 32 956
Fragments arranged by size:
3170 956 32 32
PpuMI rG'GwC_Cy
Cuts at: 1001 1001
Size: 4190
PstI C_TGCA'G
Cuts at: 806 1237 1922 806
Size: 431 685 3074
Fragments arranged by size:
3074 685 431
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4190
PvuII CAG'CTG
Cuts at: 1615 1975 1615
Size: 360 3830
RsrII CG'GwC_CG
Cuts at: 2385 2385
Size: 4190
SacI G_AGCT'C
Cuts at: 1204 1204
Size: 4190
SalI G'TCGA_C
Cuts at: 1227 1227
Size: 4190
SapI GCTCTTCn'nnn_
Cuts at: 190 931 2213 2423 190
Size: 741 1282 210 1957
Fragments arranged by size:
1957 1282 741 210
SbfI CC_TGCA'GG
Cuts at: 1237 1237
Size: 4190
ScaI AGT'ACT
Cuts at: 637 3707 637
Size: 3070 1120
SexAI A'CCwGG_T
Cuts at: 649 649
Size: 4190
SfcI C'TryA_G
Cuts at: 33 802 1233 1918 2911 3102 33
Size: 769 431 685 993 191 1121
Fragments arranged by size:
1121 993 769 685 431 191
SfoI GGC'GCC
Cuts at: 768 1622 1870 768
Size: 854 248 3088
Fragments arranged by size:
3088 854 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
SgrAI Cr'CCGG_yG
Cuts at: 794 794
Size: 4190
SmaI CCC'GGG
Cuts at: 1212 1212
Size: 4190
SmlI C'TyrA_G
Cuts at: 1345 2260 2752 3014 1345
Size: 915 492 262 2521
Fragments arranged by size:
2521 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4190
SphI G_CATG'C
Cuts at: 1243 1743 1243
Size: 500 3690
StyI C'CwwG_G
Cuts at: 482 507 1066 1324 1678 482
Size: 25 559 258 354 2994
Fragments arranged by size:
2994 559 354 258 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 273 1773 1906 2365 2574 273
Size: 1500 133 459 209 1889
Fragments arranged by size:
1889 1500 459 209 133
TatI w'GTAC_w
Cuts at: 635 3705 635
Size: 3070 1120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 458 490 802 834 3707 3739 458
Size: 32 312 32 2873 32 909
Fragments arranged by size:
2873 909 312 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1987 1987
Size: 4190
XbaI T'CTAG_A
Cuts at: 1221 1221
Size: 4190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1685 1685
Size: 4190
XmaI C'CCGG_G
Cuts at: 1210 1210
Size: 4190
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BsrGI BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI