Restriction map for
pF1KB8965
(Circular) (Six-base) MAPSORT of: pF1KB8965.seq Check: 6582 from: 1 to: 4175
pF1KB8965 4175 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 686 686
Size: 4175
AccI GT'mk_AC
Cuts at: 818 1213 818
Size: 395 3780
Acc65I G'GTAC_C
Cuts at: 1191 1191
Size: 4175
AclI AA'CG_TT
Cuts at: 4015 4015
Size: 4175
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2000 2432 3179 3447 2000
Size: 432 747 268 2728
Fragments arranged by size:
2728 747 432 268
AflIII A'CryG_T
Cuts at: 1527 2631 3608 1527
Size: 1104 977 2094
Fragments arranged by size:
2094 1104 977
AgeI A'CCGG_T
Cuts at: 2563 2563
Size: 4175
AhdI GACnn_n'nnGTC
Cuts at: 117 117
Size: 4175
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 670 670
Size: 4175
AlwNI CAG_nnn'CTG
Cuts at: 264 900 1480 3047 264
Size: 636 580 1567 1392
Fragments arranged by size:
1567 1392 636 580
ApaLI G'TGCA_C
Cuts at: 2945 2945
Size: 4175
ApoI r'AATT_y
Cuts at: 1179 3528 3784 1179
Size: 2349 256 1570
Fragments arranged by size:
2349 1570 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4175
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
AvaI C'yCGr_G
Cuts at: 822 1195 822
Size: 373 3802
BamHI G'GATC_C
Cuts at: 1200 1200
Size: 4175
BanI G'GyrC_C
Cuts at: 164 669 759 787 874 1191 1605 1853 1888
Size: 505 90 28 87 317 414 248 35
Cuts at: 1888 164
Size: 2451
Fragments arranged by size:
2451 505 414 317 248 90 87 35
28
BanII G_rGCy'C
Cuts at: 295 439 515 556 829 1120 1189 2219 295
Size: 144 76 41 273 291 69 1030 2251
Fragments arranged by size:
2251 1030 291 273 144 76 69 41
BbeI G_GCGC'C
Cuts at: 168 791 878 1609 1857 168
Size: 623 87 731 248 2486
Fragments arranged by size:
2486 731 623 248 87
BbsI GAAGACnn'nnnn_
Cuts at: 151 1059 151
Size: 908 3267
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 455 489 455
Size: 34 4141
BciVI GTATCCnnnnn_n'
Cuts at: 2073 2834 4175 2073
Size: 761 1341 2073
Fragments arranged by size:
2073 1341 761
BclI T'GATC_A
Cuts at: 1695 1695
Size: 4175
BglII A'GATC_T
Cuts at: 251 1690 3727 251
Size: 1439 2037 699
Fragments arranged by size:
2037 1439 699
BlpI GC'TnA_GC
Cuts at: 244 1287 244
Size: 1043 3132
Bme1580I G_kGCm'C
Cuts at: 577 674 764 1800 1893 2949 577
Size: 97 90 1036 93 1056 1803
Fragments arranged by size:
1803 1056 1036 97 93 90
BmrI ACTGGGnnnn_n'
Cuts at: 1387 1468 1803 3947 1387
Size: 81 335 2144 1615
Fragments arranged by size:
2144 1615 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 453 2542 453
Size: 2089 2086
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 979 616
Size: 363 3812
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 1351 2230 2722 3020 469
Size: 882 879 492 298 1624
Fragments arranged by size:
1624 882 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3879 3879
Size: 4175
BsaAI yAC'GTr
Cuts at: 1528 1528
Size: 4175
BsaBI GATnn'nnATC
Cuts at: 6 1689 3726 6
Size: 1683 2037 455
Fragments arranged by size:
2037 1683 455
BsaHI Gr'CG_yC
Cuts at: 165 788 853 875 1087 1606 1854 2556 4052
Size: 623 65 22 212 519 248 702 1496
Cuts at: 4052 165
Size: 288
Fragments arranged by size:
1496 702 623 519 288 248 212 65
22
BsaWI w'CCGG_w
Cuts at: 1364 1885 2563 2837 2984 3395 1364
Size: 521 678 274 147 411 2144
Fragments arranged by size:
2144 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 952 982 952
Size: 30 4145
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 445 445
Size: 4175
BseYI C'CCAG_C
Cuts at: 658 996 1039 1600 2358 2935 3431 658
Size: 338 43 561 758 577 496 1402
Fragments arranged by size:
1402 758 577 561 496 338 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 958 958
Size: 4175
BsiEI CG_ry'CG
Cuts at: 104 454 560 731 1109 1717 1763 2971 104
Size: 350 106 171 378 608 46 1208 1308
Fragments arranged by size:
1308 1208 608 378 350 171 106 46
BsiHKAI G_wGCw'C
Cuts at: 1189 1967 2157 2949 1189
Size: 778 190 792 2415
Fragments arranged by size:
2415 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 295 439 515 556 577 674 764 829 1120
Size: 144 76 41 21 97 90 65 291
Cuts at: 1120 1189 1800 1893 1967 2157 2219 2949 295
Size: 69 611 93 74 190 62 730 1521
Fragments arranged by size:
1521 730 611 291 190 144 97 93
90 76 74 69 65 62 41 21
BspEI T'CCGG_A
Cuts at: 3395 3395
Size: 4175
BspHI T'CATG_A
Cuts at: 3351 3351
Size: 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 686 1125 1225 1439 1741 2122 686
Size: 439 100 214 302 381 2739
Fragments arranged by size:
2739 439 381 302 214 100
BsrBI CCG'CTC
Cuts at: 50 2467 2521 4005 4170 50
Size: 2417 54 1484 165 55
Fragments arranged by size:
2417 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2087 2087
Size: 4175
BsrFI r'CCGG_y
Cuts at: 154 559 730 784 790 1108 2173 2354 2563
Size: 405 171 54 6 318 1065 181 209
Cuts at: 2563 154
Size: 1766
Fragments arranged by size:
1766 1065 405 318 209 181 171 54
6
BsrGI T'GTAC_A
Cuts at: 494 494
Size: 4175
BssSI C'ACGA_G
Cuts at: 570 2446 2804 570
Size: 1876 358 1941
Fragments arranged by size:
1941 1876 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1552 1552
Size: 4175
BstBI TT'CG_AA
Cuts at: 2536 2536
Size: 4175
BstEII G'GTnAC_C
Cuts at: 1137 1137
Size: 4175
BstXI CCAn_nnnn'nTGG
Cuts at: 588 588
Size: 4175
BstYI r'GATC_y
Cuts at: 1 251 1200 1667 1690 2025 2271 3283 3369
Size: 250 949 467 23 335 246 1012 86
Cuts at: 3369 3381 3727 1
Size: 12 346 449
Fragments arranged by size:
1012 949 467 449 346 335 250 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 857 917 1018 1082 107
Size: 750 60 101 64 3200
Fragments arranged by size:
3200 750 101 64 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 177 2125 2306 2469 177
Size: 1948 181 163 1883
Fragments arranged by size:
1948 1883 181 163
BtsI GCAGTG_nn'
Cuts at: 1418 1418
Size: 4175
DraI TTT'AAA
Cuts at: 1174 1174
Size: 4175
DraIII CAC_nnn'GTG
Cuts at: 669 669
Size: 4175
DrdI GACnn_nn'nnGTC
Cuts at: 1881 2739 1881
Size: 858 3317
EaeI y'GGCC_r
Cuts at: 209 410 451 557 621 693 728 841 920
Size: 201 41 106 64 72 35 113 79
Cuts at: 920 1106 1760 1934 2325 2352 3697 209
Size: 186 654 174 391 27 1345 687
Fragments arranged by size:
1345 687 654 391 201 186 174 113
106 79 72 64 41 35 27
EagI C'GGCC_G
Cuts at: 451 557 728 1106 1760 451
Size: 106 171 378 654 2866
Fragments arranged by size:
2866 654 378 171 106
EarI CTCTTCn'nnn_
Cuts at: 1098 2198 2408 1098
Size: 1100 210 2865
Fragments arranged by size:
2865 1100 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2383 2693 2839 3985 2383
Size: 310 146 1146 2573
Fragments arranged by size:
2573 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1187 1187
Size: 4175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 979 2000 2432 3179 3447 616
Size: 363 1021 432 747 268 1344
Fragments arranged by size:
1344 1021 747 432 363 268
EcoO109I rG'GnC_Cy
Cuts at: 366 1314 366
Size: 948 3227
EcoRI G'AATT_C
Cuts at: 1179 1179
Size: 4175
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2194 2194
Size: 4175
FspI TGC'GCA
Cuts at: 1956 1956
Size: 4175
HaeII r_GCGC'y
Cuts at: 168 237 484 595 791 865 878 1609 1857
Size: 69 247 111 196 74 13 731 248
Cuts at: 1857 2879 3858 168
Size: 1022 979 485
Fragments arranged by size:
1022 979 731 485 248 247 196 111
74 69 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 2017 2047 2049 2079 103
Size: 32 1882 30 2 30 2199
Fragments arranged by size:
2199 1882 32 30 30 2
HincII GTy'rAC
Cuts at: 1214 1214
Size: 4175
KasI G'GCGC_C
Cuts at: 164 787 874 1605 1853 164
Size: 623 87 731 248 2486
Fragments arranged by size:
2486 731 623 248 87
KpnI G_GTAC'C
Cuts at: 1195 1195
Size: 4175
MluI A'CGCG_T
Cuts at: 3608 3608
Size: 4175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 710 2846 3030 710
Size: 2136 184 1855
Fragments arranged by size:
2136 1855 184
MscI TGG'CCA
Cuts at: 211 1936 211
Size: 1725 2450
MslI CAynn'nnrTG
Cuts at: 205 2164 2291 205
Size: 1959 127 2089
Fragments arranged by size:
2089 1959 127
MspA1I CmG'CkG
Cuts at: 774 859 872 919 1020 1286 1600 1960 2973
Size: 85 13 47 101 266 314 360 1013
Cuts at: 2973 3218 774
Size: 245 1731
Fragments arranged by size:
1731 1013 360 314 266 245 101 85
47 13
NaeI GCC'GGC
Cuts at: 156 561 732 1110 2356 156
Size: 405 171 378 1246 1975
Fragments arranged by size:
1975 1246 405 378 171
NarI GG'CG_CC
Cuts at: 165 788 875 1606 1854 165
Size: 623 87 731 248 2486
Fragments arranged by size:
2486 731 623 248 87
NcoI C'CATG_G
Cuts at: 107 1082 107
Size: 975 3200
NgoMIV G'CCGG_C
Cuts at: 154 559 730 1108 2354 154
Size: 405 171 378 1246 1975
Fragments arranged by size:
1975 1246 405 378 171
NotI GC'GGCC_GC
Cuts at: 451 451
Size: 4175
NspI r_CATG'y
Cuts at: 1047 1228 1728 2635 1047
Size: 181 500 907 2587
Fragments arranged by size:
2587 907 500 181
PciI A'CATG_T
Cuts at: 2631 2631
Size: 4175
PfoI T'CCnGG_A
Cuts at: 530 530
Size: 4175
PmeI GTTT'AAAC
Cuts at: 1174 1174
Size: 4175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 409 441 3314 3346 409
Size: 32 2873 32 1238
Fragments arranged by size:
2873 1238 32 32
PstI C_TGCA'G
Cuts at: 269 1222 1907 269
Size: 953 685 2537
Fragments arranged by size:
2537 953 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4175
PvuII CAG'CTG
Cuts at: 1600 1960 1600
Size: 360 3815
RsrII CG'GwC_CG
Cuts at: 2370 2370
Size: 4175
SacI G_AGCT'C
Cuts at: 1189 1189
Size: 4175
SacII CC_GC'GG
Cuts at: 860 920 1021 860
Size: 60 101 4014
Fragments arranged by size:
4014 101 60
SalI G'TCGA_C
Cuts at: 1212 1212
Size: 4175
SapI GCTCTTCn'nnn_
Cuts at: 2198 2408 2198
Size: 210 3965
SbfI CC_TGCA'GG
Cuts at: 1222 1222
Size: 4175
ScaI AGT'ACT
Cuts at: 3692 3692
Size: 4175
SfcI C'TryA_G
Cuts at: 33 265 1218 1903 2896 3087 33
Size: 232 953 685 993 191 1121
Fragments arranged by size:
1121 993 953 685 232 191
SfoI GGC'GCC
Cuts at: 166 789 876 1607 1855 166
Size: 623 87 731 248 2486
Fragments arranged by size:
2486 731 623 248 87
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
SgrAI Cr'CCGG_yG
Cuts at: 784 790 784
Size: 6 4169
SmaI CCC'GGG
Cuts at: 1197 1197
Size: 4175
SmlI C'TyrA_G
Cuts at: 484 1330 2245 2737 2999 484
Size: 846 915 492 262 1660
Fragments arranged by size:
1660 915 846 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4175
SphI G_CATG'C
Cuts at: 1228 1728 1228
Size: 500 3675
StyI C'CwwG_G
Cuts at: 107 134 362 369 1082 1309 1663 107
Size: 27 228 7 713 227 354 2619
Fragments arranged by size:
2619 713 354 228 227 27 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 240 1067 1758 1891 2350 2559 240
Size: 827 691 133 459 209 1856
Fragments arranged by size:
1856 827 691 459 209 133
TatI w'GTAC_w
Cuts at: 494 3690 494
Size: 3196 979
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 667 699 3692 3724 667
Size: 32 2993 32 1118
Fragments arranged by size:
2993 1118 32 32
Tth111I GACn'n_nGTC
Cuts at: 1972 1972
Size: 4175
XbaI T'CTAG_A
Cuts at: 1206 1206
Size: 4175
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 954 1670 954
Size: 716 3459
XmaI C'CCGG_G
Cuts at: 1195 1195
Size: 4175
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsiWI
BsmI BsmBI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI