Restriction map for
pF1KB8964
(Circular) (Six-base) MAPSORT of: pF1KB8964.seq Check: 3342 from: 1 to: 4163
pF1KB8964 4163 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 324 324
Size: 4163
AccI GT'mk_AC
Cuts at: 179 1115 1201 179
Size: 936 86 3141
Fragments arranged by size:
3141 936 86
Acc65I G'GTAC_C
Cuts at: 1179 1179
Size: 4163
AclI AA'CG_TT
Cuts at: 4003 4003
Size: 4163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1003 1115 1988 2420 3167 3435 1003
Size: 112 873 432 747 268 1731
Fragments arranged by size:
1731 873 747 432 268 112
AfeI AGC'GCT
Cuts at: 586 586
Size: 4163
AflIII A'CryG_T
Cuts at: 704 1515 2619 3596 704
Size: 811 1104 977 1271
Fragments arranged by size:
1271 1104 977 811
AgeI A'CCGG_T
Cuts at: 2551 2551
Size: 4163
AlwNI CAG_nnn'CTG
Cuts at: 316 814 880 1468 3035 316
Size: 498 66 588 1567 1444
Fragments arranged by size:
1567 1444 588 498 66
ApaI G_GGCC'C
Cuts at: 335 390 684 335
Size: 55 294 3814
Fragments arranged by size:
3814 294 55
ApaLI G'TGCA_C
Cuts at: 406 2933 406
Size: 2527 1636
ApoI r'AATT_y
Cuts at: 1167 3516 3772 1167
Size: 2349 256 1558
Fragments arranged by size:
2349 1558 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4163
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
AvaI C'yCGr_G
Cuts at: 390 1183 390
Size: 793 3370
BamHI G'GATC_C
Cuts at: 1188 1188
Size: 4163
BanI G'GyrC_C
Cuts at: 303 504 1179 1593 1841 1876 303
Size: 201 675 414 248 35 2590
Fragments arranged by size:
2590 675 414 248 201 35
BanII G_rGCy'C
Cuts at: 118 272 335 390 673 684 918 971 1177
Size: 154 63 55 283 11 234 53 206
Cuts at: 1177 2207 118
Size: 1030 2074
Fragments arranged by size:
2074 1030 283 234 206 154 63 55
53 11
BbeI G_GCGC'C
Cuts at: 508 1597 1845 508
Size: 1089 248 2826
Fragments arranged by size:
2826 1089 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4129
BciVI GTATCCnnnnn_n'
Cuts at: 2061 2822 4163 2061
Size: 761 1341 2061
Fragments arranged by size:
2061 1341 761
BclI T'GATC_A
Cuts at: 1683 1683
Size: 4163
BfrBI ATG'CAT
Cuts at: 1153 1153
Size: 4163
BglII A'GATC_T
Cuts at: 1678 3715 1678
Size: 2037 2126
BlpI GC'TnA_GC
Cuts at: 1275 1275
Size: 4163
Bme1580I G_kGCm'C
Cuts at: 203 335 390 410 684 1788 1881 2937 203
Size: 132 55 20 274 1104 93 1056 1429
Fragments arranged by size:
1429 1104 1056 274 132 93 55 20
BmgBI CAC'GTC
Cuts at: 1071 1071
Size: 4163
BmrI ACTGGGnnnn_n'
Cuts at: 190 687 1375 1456 1791 3935 190
Size: 497 688 81 335 2144 418
Fragments arranged by size:
2144 688 497 418 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 198 2530 198
Size: 2332 1831
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 857 985 1130 772
Size: 85 128 145 3805
Fragments arranged by size:
3805 145 128 85
Bpu10I CC'TnA_GC
Cuts at: 696 881 696
Size: 185 3978
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 546 1339 2218 2710 3008 546
Size: 793 879 492 298 1701
Fragments arranged by size:
1701 879 793 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3867 3867
Size: 4163
BsaAI yAC'GTr
Cuts at: 1516 1516
Size: 4163
BsaBI GATnn'nnATC
Cuts at: 6 1677 3714 6
Size: 1671 2037 455
Fragments arranged by size:
2037 1671 455
BsaHI Gr'CG_yC
Cuts at: 505 1594 1842 2544 4040 505
Size: 1089 248 702 1496 628
Fragments arranged by size:
1496 1089 702 628 248
BsaWI w'CCGG_w
Cuts at: 1352 1873 2551 2825 2972 3383 1352
Size: 521 678 274 147 411 2132
Fragments arranged by size:
2132 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1003 1033 1003
Size: 30 4133
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 769 769
Size: 4163
BseYI C'CCAG_C
Cuts at: 111 309 691 860 1588 2346 2923 3419 111
Size: 198 382 169 728 758 577 496 855
Fragments arranged by size:
855 758 728 577 496 382 198 169
BsiEI CG_ry'CG
Cuts at: 104 1705 1751 2959 104
Size: 1601 46 1208 1308
Fragments arranged by size:
1601 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 410 971 1177 1955 2145 2937 410
Size: 561 206 778 190 792 1636
Fragments arranged by size:
1636 792 778 561 206 190
Bsp1286I G_dGCh'C
Cuts at: 118 203 272 335 390 410 673 684 918
Size: 85 69 63 55 20 263 11 234
Cuts at: 918 971 1177 1788 1881 1955 2145 2207 2937
Size: 53 206 611 93 74 190 62 730
Cuts at: 2937 118
Size: 1344
Fragments arranged by size:
1344 730 611 263 234 206 190 93
85 74 69 63 62 55 53 20
11
BspEI T'CCGG_A
Cuts at: 3383 3383
Size: 4163
BspHI T'CATG_A
Cuts at: 3339 3339
Size: 4163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 324 734 1213 1427 1729 2110 324
Size: 410 479 214 302 381 2377
Fragments arranged by size:
2377 479 410 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1139 2455 2509 3993 4158 50
Size: 1089 1316 54 1484 165 55
Fragments arranged by size:
1484 1316 1089 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2075 2075
Size: 4163
BsrFI r'CCGG_y
Cuts at: 186 2161 2342 2551 186
Size: 1975 181 209 1798
Fragments arranged by size:
1975 1798 209 181
BssSI C'ACGA_G
Cuts at: 2434 2792 2434
Size: 358 3805
BstAPI GCAn_nnn'nTGC
Cuts at: 1540 1540
Size: 4163
BstBI TT'CG_AA
Cuts at: 2524 2524
Size: 4163
BstEII G'GTnAC_C
Cuts at: 189 189
Size: 4163
BstYI r'GATC_y
Cuts at: 1 1188 1655 1678 2013 2259 3271 3357 3369
Size: 1187 467 23 335 246 1012 86 12
Cuts at: 3369 3715 1
Size: 346 449
Fragments arranged by size:
1187 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 291 887 291
Size: 596 3567
BtgI C'CryG_G
Cuts at: 206 488 836 206
Size: 282 348 3533
Fragments arranged by size:
3533 348 282
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2113 2294 2457 2113
Size: 181 163 3819
Fragments arranged by size:
3819 181 163
BtsI GCAGTG_nn'
Cuts at: 283 1406 283
Size: 1123 3040
DraI TTT'AAA
Cuts at: 1162 1162
Size: 4163
DrdI GACnn_nn'nnGTC
Cuts at: 631 1869 2727 631
Size: 1238 858 2067
Fragments arranged by size:
2067 1238 858
EaeI y'GGCC_r
Cuts at: 143 491 839 1748 1922 2313 2340 3685 143
Size: 348 348 909 174 391 27 1345 621
Fragments arranged by size:
1345 909 621 391 348 348 174 27
EagI C'GGCC_G
Cuts at: 1748 1748
Size: 4163
EarI CTCTTCn'nnn_
Cuts at: 562 2186 2396 562
Size: 1624 210 2329
Fragments arranged by size:
2329 1624 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2371 2681 2827 3973 2371
Size: 310 146 1146 2561
Fragments arranged by size:
2561 1146 310 146
EcoICRI GAG'CTC
Cuts at: 969 1175 969
Size: 206 3957
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 857 985 1003 1115 1130 1988 2420 3167
Size: 85 128 18 112 15 858 432 747
Cuts at: 3167 3435 772
Size: 268 1500
Fragments arranged by size:
1500 858 747 432 268 128 112 85
18 15
EcoO109I rG'GnC_Cy
Cuts at: 295 331 386 387 394 681 1125 1302 295
Size: 36 55 1 7 287 444 177 3156
Fragments arranged by size:
3156 444 287 177 55 36 7 1
EcoRI G'AATT_C
Cuts at: 1167 1167
Size: 4163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2182 2182
Size: 4163
FspI TGC'GCA
Cuts at: 1944 1944
Size: 4163
HaeII r_GCGC'y
Cuts at: 508 588 1597 1845 2867 3846 508
Size: 80 1009 248 1022 979 825
Fragments arranged by size:
1022 1009 979 825 248 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 222 254 913 945 2005 2035 2037 2067 222
Size: 32 659 32 1060 30 2 30 2318
Fragments arranged by size:
2318 1060 659 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1202 1202
Size: 4163
KasI G'GCGC_C
Cuts at: 504 1593 1841 504
Size: 1089 248 2826
Fragments arranged by size:
2826 1089 248
KpnI G_GTAC'C
Cuts at: 1183 1183
Size: 4163
MluI A'CGCG_T
Cuts at: 3596 3596
Size: 4163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2834 3018 2834
Size: 184 3979
MscI TGG'CCA
Cuts at: 493 1924 493
Size: 1431 2732
MslI CAynn'nnrTG
Cuts at: 487 2152 2279 487
Size: 1665 127 2371
Fragments arranged by size:
2371 1665 127
MspA1I CmG'CkG
Cuts at: 359 779 838 1274 1588 1948 2961 3206 359
Size: 420 59 436 314 360 1013 245 1316
Fragments arranged by size:
1316 1013 436 420 360 314 245 59
NaeI GCC'GGC
Cuts at: 2344 2344
Size: 4163
NarI GG'CG_CC
Cuts at: 505 1594 1842 505
Size: 1089 248 2826
Fragments arranged by size:
2826 1089 248
NcoI C'CATG_G
Cuts at: 206 488 206
Size: 282 3881
NgoMIV G'CCGG_C
Cuts at: 2342 2342
Size: 4163
NsiI A_TGCA'T
Cuts at: 1155 1155
Size: 4163
NspI r_CATG'y
Cuts at: 708 1216 1716 2623 708
Size: 508 500 907 2248
Fragments arranged by size:
2248 907 508 500
PasI CC'CwG_GG
Cuts at: 262 327 894 973 262
Size: 65 567 79 3452
Fragments arranged by size:
3452 567 79 65
PciI A'CATG_T
Cuts at: 704 2619 704
Size: 1915 2248
PflMI CCAn_nnn'nTGG
Cuts at: 141 141
Size: 4163
PmeI GTTT'AAAC
Cuts at: 1162 1162
Size: 4163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3302 3334 3302
Size: 32 4131
PpuMI rG'GwC_Cy
Cuts at: 295 394 295
Size: 99 4064
PspOMI G'GGCC_C
Cuts at: 331 386 680 331
Size: 55 294 3814
Fragments arranged by size:
3814 294 55
PstI C_TGCA'G
Cuts at: 321 1210 1895 321
Size: 889 685 2589
Fragments arranged by size:
2589 889 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4163
PvuII CAG'CTG
Cuts at: 1588 1948 1588
Size: 360 3803
RsrII CG'GwC_CG
Cuts at: 2358 2358
Size: 4163
SacI G_AGCT'C
Cuts at: 971 1177 971
Size: 206 3957
SacII CC_GC'GG
Cuts at: 839 839
Size: 4163
SalI G'TCGA_C
Cuts at: 1200 1200
Size: 4163
SapI GCTCTTCn'nnn_
Cuts at: 2186 2396 2186
Size: 210 3953
SbfI CC_TGCA'GG
Cuts at: 1210 1210
Size: 4163
ScaI AGT'ACT
Cuts at: 3680 3680
Size: 4163
SfcI C'TryA_G
Cuts at: 33 317 1206 1891 2884 3075 33
Size: 284 889 685 993 191 1121
Fragments arranged by size:
1121 993 889 685 284 191
SfoI GGC'GCC
Cuts at: 506 1595 1843 506
Size: 1089 248 2826
Fragments arranged by size:
2826 1089 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
SgrAI Cr'CCGG_yG
Cuts at: 186 186
Size: 4163
SmaI CCC'GGG
Cuts at: 392 1185 392
Size: 793 3370
SmlI C'TyrA_G
Cuts at: 525 1318 2233 2725 2987 525
Size: 793 915 492 262 1701
Fragments arranged by size:
1701 915 793 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4163
SphI G_CATG'C
Cuts at: 1216 1716 1216
Size: 500 3663
StuI AGG'CCT
Cuts at: 347 347
Size: 4163
StyI C'CwwG_G
Cuts at: 206 488 1297 1651 206
Size: 282 809 354 2718
Fragments arranged by size:
2718 809 354 282
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 340 1746 1879 2338 2547 340
Size: 1406 133 459 209 1956
Fragments arranged by size:
1956 1406 459 209 133
TatI w'GTAC_w
Cuts at: 3678 3678
Size: 4163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1003 1035 3680 3712 1003
Size: 32 2645 32 1454
Fragments arranged by size:
2645 1454 32 32
Tth111I GACn'n_nGTC
Cuts at: 810 1960 810
Size: 1150 3013
XbaI T'CTAG_A
Cuts at: 1194 1194
Size: 4163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1068 1658 1068
Size: 590 3573
XmaI C'CCGG_G
Cuts at: 390 1183 390
Size: 793 3370
XmnI GAAnn'nnTTC
Cuts at: 742 742
Size: 4163
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BglI BmtI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstXI BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PfoI PmlI PshAI PsiI
PspXI PsrI SanDI SexAI SfiI SnaBI SrfI SspI
SwaI XhoI ZraI