Restriction map for
pF1KB8963
(Circular) (Six-base) MAPSORT of: pF1KB8963.seq Check: 7347 from: 1 to: 4163
pF1KB8963 4163 bp
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AccI GT'mk_AC
Cuts at: 1201 1201
Size: 4163
Acc65I G'GTAC_C
Cuts at: 1179 1179
Size: 4163
AclI AA'CG_TT
Cuts at: 4003 4003
Size: 4163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 792 1988 2420 3167 3435 792
Size: 1196 432 747 268 1520
Fragments arranged by size:
1520 1196 747 432 268
AflIII A'CryG_T
Cuts at: 1515 2619 3596 1515
Size: 1104 977 2082
Fragments arranged by size:
2082 1104 977
AgeI A'CCGG_T
Cuts at: 2551 2551
Size: 4163
AleI CACnn'nnGTG
Cuts at: 220 220
Size: 4163
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 145 145
Size: 4163
AlwNI CAG_nnn'CTG
Cuts at: 1468 3035 1468
Size: 1567 2596
ApaLI G'TGCA_C
Cuts at: 636 2933 636
Size: 2297 1866
ApoI r'AATT_y
Cuts at: 813 1008 1167 3516 3772 813
Size: 195 159 2349 256 1204
Fragments arranged by size:
2349 1204 256 195 159
AseI AT'TA_AT
Cuts at: 20 20
Size: 4163
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
AvaI C'yCGr_G
Cuts at: 621 1183 621
Size: 562 3601
AvrII C'CTAG_G
Cuts at: 916 916
Size: 4163
BamHI G'GATC_C
Cuts at: 740 920 1188 740
Size: 180 268 3715
Fragments arranged by size:
3715 268 180
BanI G'GyrC_C
Cuts at: 912 1179 1593 1841 1876 912
Size: 267 414 248 35 3199
Fragments arranged by size:
3199 414 267 248 35
BanII G_rGCy'C
Cuts at: 864 1177 2207 864
Size: 313 1030 2820
Fragments arranged by size:
2820 1030 313
BbeI G_GCGC'C
Cuts at: 1597 1845 1597
Size: 248 3915
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4129
BciVI GTATCCnnnnn_n'
Cuts at: 2061 2822 4163 2061
Size: 761 1341 2061
Fragments arranged by size:
2061 1341 761
BclI T'GATC_A
Cuts at: 707 1683 707
Size: 976 3187
BglI GCCn_nnn'nGGC
Cuts at: 180 180
Size: 4163
BglII A'GATC_T
Cuts at: 1678 3715 1678
Size: 2037 2126
BlpI GC'TnA_GC
Cuts at: 312 1275 312
Size: 963 3200
Bme1580I G_kGCm'C
Cuts at: 640 1788 1881 2937 640
Size: 1148 93 1056 1866
Fragments arranged by size:
1866 1148 1056 93
BmgBI CAC'GTC
Cuts at: 1086 1086
Size: 4163
BmrI ACTGGGnnnn_n'
Cuts at: 642 1375 1456 1791 3935 642
Size: 733 81 335 2144 870
Fragments arranged by size:
2144 870 733 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2530 2530
Size: 4163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 736 595
Size: 141 4022
Bpu10I CC'TnA_GC
Cuts at: 280 433 280
Size: 153 4010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 1339 2218 2710 3008 490
Size: 849 879 492 298 1645
Fragments arranged by size:
1645 879 849 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3867 3867
Size: 4163
BsaAI yAC'GTr
Cuts at: 549 1516 549
Size: 967 3196
BsaBI GATnn'nnATC
Cuts at: 6 1677 3714 6
Size: 1671 2037 455
Fragments arranged by size:
2037 1671 455
BsaHI Gr'CG_yC
Cuts at: 187 1594 1842 2544 4040 187
Size: 1407 248 702 1496 310
Fragments arranged by size:
1496 1407 702 310 248
BsaWI w'CCGG_w
Cuts at: 1352 1873 2551 2825 2972 3383 1352
Size: 521 678 274 147 411 2132
Fragments arranged by size:
2132 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 544 854 544
Size: 310 3853
BseYI C'CCAG_C
Cuts at: 143 857 1588 2346 2923 3419 143
Size: 714 731 758 577 496 887
Fragments arranged by size:
887 758 731 714 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 620 385
Size: 235 3928
BsiEI CG_ry'CG
Cuts at: 104 581 1705 1751 2959 104
Size: 477 1124 46 1208 1308
Fragments arranged by size:
1308 1208 1124 477 46
BsiHKAI G_wGCw'C
Cuts at: 155 554 640 1177 1955 2145 2937 155
Size: 399 86 537 778 190 792 1381
Fragments arranged by size:
1381 792 778 537 399 190 86
BsmBI CGTCTCn'nnnn_
Cuts at: 1118 1118
Size: 4163
Bsp1286I G_dGCh'C
Cuts at: 155 554 640 864 1177 1788 1881 1955 2145
Size: 399 86 224 313 611 93 74 190
Cuts at: 2145 2207 2937 155
Size: 62 730 1381
Fragments arranged by size:
1381 730 611 399 313 224 190 93
86 74 62
BspEI T'CCGG_A
Cuts at: 3383 3383
Size: 4163
BspHI T'CATG_A
Cuts at: 3339 3339
Size: 4163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1213 1427 1729 2110 1213
Size: 214 302 381 3266
Fragments arranged by size:
3266 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2455 2509 3993 4158 50
Size: 2405 54 1484 165 55
Fragments arranged by size:
2405 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2075 2075
Size: 4163
BsrFI r'CCGG_y
Cuts at: 463 2161 2342 2551 463
Size: 1698 181 209 2075
Fragments arranged by size:
2075 1698 209 181
BsrGI T'GTAC_A
Cuts at: 521 1048 521
Size: 527 3636
BssHII G'CGCG_C
Cuts at: 125 167 125
Size: 42 4121
BssSI C'ACGA_G
Cuts at: 177 633 2434 2792 177
Size: 456 1801 358 1548
Fragments arranged by size:
1801 1548 456 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1540 1540
Size: 4163
BstBI TT'CG_AA
Cuts at: 2524 2524
Size: 4163
BstXI CCAn_nnnn'nTGG
Cuts at: 225 415 678 831 225
Size: 190 263 153 3557
Fragments arranged by size:
3557 263 190 153
BstYI r'GATC_y
Cuts at: 1 626 740 920 1188 1655 1678 2013 2259
Size: 625 114 180 268 467 23 335 246
Cuts at: 2259 3271 3357 3369 3715 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 625 467 449 346 335 268 246
180 114 86 23 12
BtgI C'CryG_G
Cuts at: 156 677 887 156
Size: 521 210 3432
Fragments arranged by size:
3432 521 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 453 657 2113 2294 2457 453
Size: 204 1456 181 163 2159
Fragments arranged by size:
2159 1456 204 181 163
BtsI GCAGTG_nn'
Cuts at: 402 1406 402
Size: 1004 3159
DraI TTT'AAA
Cuts at: 1162 1162
Size: 4163
DrdI GACnn_nn'nnGTC
Cuts at: 1869 2727 1869
Size: 858 3305
EaeI y'GGCC_r
Cuts at: 335 578 884 941 1088 1748 1922 2313 2340
Size: 243 306 57 147 660 174 391 27
Cuts at: 2340 3685 335
Size: 1345 813
Fragments arranged by size:
1345 813 660 391 306 243 174 147
57 27
EagI C'GGCC_G
Cuts at: 578 1748 578
Size: 1170 2993
EarI CTCTTCn'nnn_
Cuts at: 2186 2396 2186
Size: 210 3953
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 180 651 2371 2681 2827 3973 180
Size: 471 1720 310 146 1146 370
Fragments arranged by size:
1720 1146 471 370 310 146
EcoICRI GAG'CTC
Cuts at: 1175 1175
Size: 4163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 736 792 1988 2420 3167 3435 595
Size: 141 56 1196 432 747 268 1323
Fragments arranged by size:
1323 1196 747 432 268 141 56
EcoO109I rG'GnC_Cy
Cuts at: 1302 1302
Size: 4163
EcoRI G'AATT_C
Cuts at: 1167 1167
Size: 4163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2182 2182
Size: 4163
FspI TGC'GCA
Cuts at: 1944 1944
Size: 4163
HaeII r_GCGC'y
Cuts at: 1597 1845 2867 3846 1597
Size: 248 1022 979 1914
Fragments arranged by size:
1914 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2005 2035 2037 2067 2005
Size: 30 2 30 4101
Fragments arranged by size:
4101 30 30 2
HincII GTy'rAC
Cuts at: 1202 1202
Size: 4163
KasI G'GCGC_C
Cuts at: 1593 1841 1593
Size: 248 3915
KpnI G_GTAC'C
Cuts at: 1183 1183
Size: 4163
MluI A'CGCG_T
Cuts at: 3596 3596
Size: 4163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2834 3018 2834
Size: 184 3979
MscI TGG'CCA
Cuts at: 337 943 1924 337
Size: 606 981 2576
Fragments arranged by size:
2576 981 606
MslI CAynn'nnrTG
Cuts at: 220 223 301 676 829 2152 2279 220
Size: 3 78 375 153 1323 127 2104
Fragments arranged by size:
2104 1323 375 153 127 78 3
MspA1I CmG'CkG
Cuts at: 158 971 1274 1588 1948 2961 3206 158
Size: 813 303 314 360 1013 245 1115
Fragments arranged by size:
1115 1013 813 360 314 303 245
NaeI GCC'GGC
Cuts at: 2344 2344
Size: 4163
NarI GG'CG_CC
Cuts at: 1594 1842 1594
Size: 248 3915
NdeI CA'TA_TG
Cuts at: 559 559
Size: 4163
NgoMIV G'CCGG_C
Cuts at: 2342 2342
Size: 4163
NspI r_CATG'y
Cuts at: 1216 1716 2623 1216
Size: 500 907 2756
Fragments arranged by size:
2756 907 500
PciI A'CATG_T
Cuts at: 2619 2619
Size: 4163
PflMI CCAn_nnn'nTGG
Cuts at: 857 857
Size: 4163
PmeI GTTT'AAAC
Cuts at: 1162 1162
Size: 4163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3302 3334 3302
Size: 32 4131
PstI C_TGCA'G
Cuts at: 1210 1895 1210
Size: 685 3478
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4163
PvuII CAG'CTG
Cuts at: 971 1588 1948 971
Size: 617 360 3186
Fragments arranged by size:
3186 617 360
RsrII CG'GwC_CG
Cuts at: 2358 2358
Size: 4163
SacI G_AGCT'C
Cuts at: 1177 1177
Size: 4163
SacII CC_GC'GG
Cuts at: 159 159
Size: 4163
SalI G'TCGA_C
Cuts at: 1200 1200
Size: 4163
SapI GCTCTTCn'nnn_
Cuts at: 2186 2396 2186
Size: 210 3953
SbfI CC_TGCA'GG
Cuts at: 1210 1210
Size: 4163
ScaI AGT'ACT
Cuts at: 3680 3680
Size: 4163
SexAI A'CCwGG_T
Cuts at: 326 326
Size: 4163
SfcI C'TryA_G
Cuts at: 33 1206 1891 2884 3075 33
Size: 1173 685 993 191 1121
Fragments arranged by size:
1173 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1595 1843 1595
Size: 248 3915
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
SmaI CCC'GGG
Cuts at: 1185 1185
Size: 4163
SmlI C'TyrA_G
Cuts at: 505 1318 2233 2725 2987 505
Size: 813 915 492 262 1681
Fragments arranged by size:
1681 915 813 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4163
SphI G_CATG'C
Cuts at: 1216 1716 1216
Size: 500 3663
StyI C'CwwG_G
Cuts at: 916 1297 1651 916
Size: 381 354 3428
Fragments arranged by size:
3428 381 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1746 1879 2338 2547 1746
Size: 133 459 209 3362
Fragments arranged by size:
3362 459 209 133
TatI w'GTAC_w
Cuts at: 521 1048 3678 521
Size: 527 2630 1006
Fragments arranged by size:
2630 1006 527
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3680 3712 3680
Size: 32 4131
Tth111I GACn'n_nGTC
Cuts at: 1960 1960
Size: 4163
XbaI T'CTAG_A
Cuts at: 1194 1194
Size: 4163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 678 951 1658 678
Size: 273 707 3183
Fragments arranged by size:
3183 707 273
XmaI C'CCGG_G
Cuts at: 1183 1183
Size: 4163
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI
PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
BaeI BbsI BbvCI BfrBI BmtI BsaXI BsiWI BsmI
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI