Restriction map for
pF1KB8962
(Circular) (Six-base) MAPSORT of: pF1KB8962.seq Check: 3126 from: 1 to: 4139
pF1KB8962 4139 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1177 1177
Size: 4139
Acc65I G'GTAC_C
Cuts at: 534 1155 534
Size: 621 3518
AclI AA'CG_TT
Cuts at: 3979 3979
Size: 4139
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1964 2396 3143 3411 1964
Size: 432 747 268 2692
Fragments arranged by size:
2692 747 432 268
AfeI AGC'GCT
Cuts at: 178 178
Size: 4139
AflIII A'CryG_T
Cuts at: 1491 2595 3572 1491
Size: 1104 977 2058
Fragments arranged by size:
2058 1104 977
AgeI A'CCGG_T
Cuts at: 2527 2527
Size: 4139
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 220 220
Size: 4139
AlwNI CAG_nnn'CTG
Cuts at: 765 948 1444 3011 765
Size: 183 496 1567 1893
Fragments arranged by size:
1893 1567 496 183
ApaI G_GGCC'C
Cuts at: 775 775
Size: 4139
ApaLI G'TGCA_C
Cuts at: 2909 2909
Size: 4139
ApoI r'AATT_y
Cuts at: 964 1143 3492 3748 964
Size: 179 2349 256 1355
Fragments arranged by size:
2349 1355 256 179
AseI AT'TA_AT
Cuts at: 20 20
Size: 4139
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
AvaI C'yCGr_G
Cuts at: 478 564 1159 478
Size: 86 595 3458
Fragments arranged by size:
3458 595 86
BamHI G'GATC_C
Cuts at: 1100 1164 1100
Size: 64 4075
BanI G'GyrC_C
Cuts at: 534 923 1155 1569 1817 1852 534
Size: 389 232 414 248 35 2821
Fragments arranged by size:
2821 414 389 248 232 35
BanII G_rGCy'C
Cuts at: 152 254 775 787 1153 2183 152
Size: 102 521 12 366 1030 2108
Fragments arranged by size:
2108 1030 521 366 102 12
BbeI G_GCGC'C
Cuts at: 1573 1821 1573
Size: 248 3891
BbvCI CC'TCA_GC
Cuts at: 677 677
Size: 4139
BciVI GTATCCnnnnn_n'
Cuts at: 2037 2798 4139 2037
Size: 761 1341 2037
Fragments arranged by size:
2037 1341 761
BclI T'GATC_A
Cuts at: 1659 1659
Size: 4139
BglI GCCn_nnn'nGGC
Cuts at: 770 770
Size: 4139
BglII A'GATC_T
Cuts at: 1654 3691 1654
Size: 2037 2102
BlpI GC'TnA_GC
Cuts at: 1251 1251
Size: 4139
Bme1580I G_kGCm'C
Cuts at: 545 775 928 1764 1857 2913 545
Size: 230 153 836 93 1056 1771
Fragments arranged by size:
1771 1056 836 230 153 93
BmrI ACTGGGnnnn_n'
Cuts at: 1351 1432 1767 3911 1351
Size: 81 335 2144 1579
Fragments arranged by size:
2144 1579 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2506 2506
Size: 4139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 810 1024 810
Size: 214 3925
Bpu10I CC'TnA_GC
Cuts at: 677 677
Size: 4139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1060 1315 2194 2686 2984 1060
Size: 255 879 492 298 2215
Fragments arranged by size:
2215 879 492 298 255
BsaI GGTCTCn'nnnn_
Cuts at: 700 3843 700
Size: 3143 996
BsaAI yAC'GTr
Cuts at: 1492 1492
Size: 4139
BsaBI GATnn'nnATC
Cuts at: 6 1653 3690 6
Size: 1647 2037 455
Fragments arranged by size:
2037 1647 455
BsaHI Gr'CG_yC
Cuts at: 589 1570 1818 2520 4016 589
Size: 981 248 702 1496 712
Fragments arranged by size:
1496 981 712 702 248
BsaWI w'CCGG_w
Cuts at: 1328 1849 2527 2801 2948 3359 1328
Size: 521 678 274 147 411 2108
Fragments arranged by size:
2108 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 185 442 874 185
Size: 257 432 3450
Fragments arranged by size:
3450 432 257
BseYI C'CCAG_C
Cuts at: 292 544 681 733 1564 2322 2899 3395 292
Size: 252 137 52 831 758 577 496 1036
Fragments arranged by size:
1036 831 758 577 496 252 137 52
BsiEI CG_ry'CG
Cuts at: 104 1681 1727 2935 104
Size: 1577 46 1208 1308
Fragments arranged by size:
1577 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 530 1153 1931 2121 2913 530
Size: 623 778 190 792 1756
Fragments arranged by size:
1756 792 778 623 190
BsiWI C'GTAC_G
Cuts at: 140 140
Size: 4139
Bsp1286I G_dGCh'C
Cuts at: 152 254 530 545 775 787 928 1153 1764
Size: 102 276 15 230 12 141 225 611
Cuts at: 1764 1857 1931 2121 2183 2913 152
Size: 93 74 190 62 730 1378
Fragments arranged by size:
1378 730 611 276 230 225 190 141
102 93 74 62 15 12
BspEI T'CCGG_A
Cuts at: 3359 3359
Size: 4139
BspHI T'CATG_A
Cuts at: 3315 3315
Size: 4139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1189 1403 1705 2086 1189
Size: 214 302 381 3242
Fragments arranged by size:
3242 381 302 214
BsrBI CCG'CTC
Cuts at: 50 908 2431 2485 3969 4134 50
Size: 858 1523 54 1484 165 55
Fragments arranged by size:
1523 1484 858 165 55 54
BsrDI GCAATG_nn'
Cuts at: 129 2051 129
Size: 1922 2217
BsrFI r'CCGG_y
Cuts at: 2137 2318 2527 2137
Size: 181 209 3749
Fragments arranged by size:
3749 209 181
BsrGI T'GTAC_A
Cuts at: 446 446
Size: 4139
BssHII G'CGCG_C
Cuts at: 654 654
Size: 4139
BssSI C'ACGA_G
Cuts at: 529 2410 2768 529
Size: 1881 358 1900
Fragments arranged by size:
1900 1881 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1516 1516
Size: 4139
BstBI TT'CG_AA
Cuts at: 617 2500 617
Size: 1883 2256
BstXI CCAn_nnnn'nTGG
Cuts at: 601 601
Size: 4139
BstYI r'GATC_y
Cuts at: 1 1100 1164 1631 1654 1989 2235 3247 3333
Size: 1099 64 467 23 335 246 1012 86
Cuts at: 3333 3345 3691 1
Size: 12 346 449
Fragments arranged by size:
1099 1012 467 449 346 335 246 86
64 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2089 2270 2433 2089
Size: 181 163 3795
Fragments arranged by size:
3795 181 163
BtsI GCAGTG_nn'
Cuts at: 1382 1382
Size: 4139
DraI TTT'AAA
Cuts at: 1138 1138
Size: 4139
DraIII CAC_nnn'GTG
Cuts at: 418 418
Size: 4139
DrdI GACnn_nn'nnGTC
Cuts at: 714 1039 1845 2703 714
Size: 325 806 858 2150
Fragments arranged by size:
2150 858 806 325
EaeI y'GGCC_r
Cuts at: 1724 1898 2289 2316 3661 1724
Size: 174 391 27 1345 2202
Fragments arranged by size:
2202 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1724 1724
Size: 4139
EarI CTCTTCn'nnn_
Cuts at: 913 2162 2372 913
Size: 1249 210 2680
Fragments arranged by size:
2680 1249 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 182 2347 2657 2803 3949 182
Size: 2165 310 146 1146 372
Fragments arranged by size:
2165 1146 372 310 146
EcoICRI GAG'CTC
Cuts at: 1151 1151
Size: 4139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 810 1024 1964 2396 3143 3411 810
Size: 214 940 432 747 268 1538
Fragments arranged by size:
1538 940 747 432 268 214
EcoO109I rG'GnC_Cy
Cuts at: 772 1278 772
Size: 506 3633
EcoRI G'AATT_C
Cuts at: 1143 1143
Size: 4139
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 4139
FspI TGC'GCA
Cuts at: 1920 1920
Size: 4139
HaeII r_GCGC'y
Cuts at: 180 999 1573 1821 2843 3822 180
Size: 819 574 248 1022 979 497
Fragments arranged by size:
1022 979 819 574 497 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 102 134 1981 2011 2013 2043 102
Size: 32 1847 30 2 30 2198
Fragments arranged by size:
2198 1847 32 30 30 2
HincII GTy'rAC
Cuts at: 1178 1178
Size: 4139
KasI G'GCGC_C
Cuts at: 1569 1817 1569
Size: 248 3891
KpnI G_GTAC'C
Cuts at: 538 1159 538
Size: 621 3518
MfeI C'AATT_G
Cuts at: 225 225
Size: 4139
MluI A'CGCG_T
Cuts at: 3572 3572
Size: 4139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 743 2810 2994 743
Size: 2067 184 1888
Fragments arranged by size:
2067 1888 184
MscI TGG'CCA
Cuts at: 1900 1900
Size: 4139
MslI CAynn'nnrTG
Cuts at: 202 2128 2255 202
Size: 1926 127 2086
Fragments arranged by size:
2086 1926 127
MspA1I CmG'CkG
Cuts at: 333 393 636 681 1250 1564 1924 2937 3182
Size: 60 243 45 569 314 360 1013 245
Cuts at: 3182 333
Size: 1290
Fragments arranged by size:
1290 1013 569 360 314 245 243 60
45
NaeI GCC'GGC
Cuts at: 2320 2320
Size: 4139
NarI GG'CG_CC
Cuts at: 1570 1818 1570
Size: 248 3891
NgoMIV G'CCGG_C
Cuts at: 2318 2318
Size: 4139
NspI r_CATG'y
Cuts at: 116 201 447 1192 1692 2599 116
Size: 85 246 745 500 907 1656
Fragments arranged by size:
1656 907 745 500 246 85
PciI A'CATG_T
Cuts at: 2595 2595
Size: 4139
PflMI CCAn_nnn'nTGG
Cuts at: 948 948
Size: 4139
PfoI T'CCnGG_A
Cuts at: 1103 1103
Size: 4139
PmeI GTTT'AAAC
Cuts at: 1138 1138
Size: 4139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3278 3310 3278
Size: 32 4107
PspOMI G'GGCC_C
Cuts at: 771 771
Size: 4139
PstI C_TGCA'G
Cuts at: 1186 1871 1186
Size: 685 3454
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4139
PvuII CAG'CTG
Cuts at: 333 681 1564 1924 333
Size: 348 883 360 2548
Fragments arranged by size:
2548 883 360 348
RsrII CG'GwC_CG
Cuts at: 2334 2334
Size: 4139
SacI G_AGCT'C
Cuts at: 1153 1153
Size: 4139
SalI G'TCGA_C
Cuts at: 1176 1176
Size: 4139
SapI GCTCTTCn'nnn_
Cuts at: 2162 2372 2162
Size: 210 3929
SbfI CC_TGCA'GG
Cuts at: 1186 1186
Size: 4139
ScaI AGT'ACT
Cuts at: 3656 3656
Size: 4139
SfcI C'TryA_G
Cuts at: 33 180 1182 1867 2860 3051 33
Size: 147 1002 685 993 191 1121
Fragments arranged by size:
1121 1002 993 685 191 147
SfoI GGC'GCC
Cuts at: 1571 1819 1571
Size: 248 3891
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
SmaI CCC'GGG
Cuts at: 1161 1161
Size: 4139
SmlI C'TyrA_G
Cuts at: 1075 1294 2209 2701 2963 1075
Size: 219 915 492 262 2251
Fragments arranged by size:
2251 915 492 262 219
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4139
SphI G_CATG'C
Cuts at: 1192 1692 1192
Size: 500 3639
StyI C'CwwG_G
Cuts at: 152 519 1273 1627 152
Size: 367 754 354 2664
Fragments arranged by size:
2664 754 367 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1722 1855 2314 2523 1722
Size: 133 459 209 3338
Fragments arranged by size:
3338 459 209 133
TatI w'GTAC_w
Cuts at: 446 3654 446
Size: 3208 931
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3656 3688 3656
Size: 32 4107
Tth111I GACn'n_nGTC
Cuts at: 1936 1936
Size: 4139
XbaI T'CTAG_A
Cuts at: 1170 1170
Size: 4139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1634 1634
Size: 4139
XmaI C'CCGG_G
Cuts at: 1159 1159
Size: 4139
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsiWI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BmgBI BmtI BsaXI BsgI
BsmI BsmBI BstEII BstZ17I Bsu36I BtgI ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI