Restriction map for
pF1KB8961
(Circular) (Six-base) MAPSORT of: pF1KB8961.seq Check: 4896 from: 1 to: 4139
pF1KB8961 4139 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 217 217
Size: 4139
AccI GT'mk_AC
Cuts at: 1177 1177
Size: 4139
Acc65I G'GTAC_C
Cuts at: 1155 1155
Size: 4139
AclI AA'CG_TT
Cuts at: 3979 3979
Size: 4139
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 862 1964 2396 3143 3411 862
Size: 1102 432 747 268 1590
Fragments arranged by size:
1590 1102 747 432 268
AfeI AGC'GCT
Cuts at: 943 943
Size: 4139
AflIII A'CryG_T
Cuts at: 998 1491 2595 3572 998
Size: 493 1104 977 1565
Fragments arranged by size:
1565 1104 977 493
AgeI A'CCGG_T
Cuts at: 2527 2527
Size: 4139
AlwNI CAG_nnn'CTG
Cuts at: 1444 3011 1444
Size: 1567 2572
ApaLI G'TGCA_C
Cuts at: 2909 2909
Size: 4139
ApoI r'AATT_y
Cuts at: 985 1143 3492 3748 985
Size: 158 2349 256 1376
Fragments arranged by size:
2349 1376 256 158
AseI AT'TA_AT
Cuts at: 20 20
Size: 4139
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
AvaI C'yCGr_G
Cuts at: 456 569 665 691 1010 1159 456
Size: 113 96 26 319 149 3436
Fragments arranged by size:
3436 319 149 113 96 26
BamHI G'GATC_C
Cuts at: 914 1164 914
Size: 250 3889
BanI G'GyrC_C
Cuts at: 121 321 542 1155 1569 1817 1852 121
Size: 200 221 613 414 248 35 2408
Fragments arranged by size:
2408 613 414 248 221 200 35
BanII G_rGCy'C
Cuts at: 413 803 1153 2183 413
Size: 390 350 1030 2369
Fragments arranged by size:
2369 1030 390 350
BbeI G_GCGC'C
Cuts at: 546 1573 1821 546
Size: 1027 248 2864
Fragments arranged by size:
2864 1027 248
BbsI GAAGACnn'nnnn_
Cuts at: 104 104
Size: 4139
BbvCI CC'TCA_GC
Cuts at: 127 804 127
Size: 677 3462
BciVI GTATCCnnnnn_n'
Cuts at: 2037 2798 4139 2037
Size: 761 1341 2037
Fragments arranged by size:
2037 1341 761
BclI T'GATC_A
Cuts at: 1659 1659
Size: 4139
BglI GCCn_nnn'nGGC
Cuts at: 164 201 527 164
Size: 37 326 3776
Fragments arranged by size:
3776 326 37
BglII A'GATC_T
Cuts at: 1654 3691 1654
Size: 2037 2102
BlpI GC'TnA_GC
Cuts at: 1251 1251
Size: 4139
Bme1580I G_kGCm'C
Cuts at: 326 1764 1857 2913 326
Size: 1438 93 1056 1552
Fragments arranged by size:
1552 1438 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 788 1351 1432 1767 3911 788
Size: 563 81 335 2144 1016
Fragments arranged by size:
2144 1016 563 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 285 2506 285
Size: 2221 1918
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 997 997
Size: 4139
Bpu10I CC'TnA_GC
Cuts at: 127 804 127
Size: 677 3462
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 991 1315 2194 2686 2984 991
Size: 324 879 492 298 2146
Fragments arranged by size:
2146 879 492 324 298
BsaI GGTCTCn'nnnn_
Cuts at: 3843 3843
Size: 4139
BsaAI yAC'GTr
Cuts at: 141 423 1492 141
Size: 282 1069 2788
Fragments arranged by size:
2788 1069 282
BsaBI GATnn'nnATC
Cuts at: 6 1653 3690 6
Size: 1647 2037 455
Fragments arranged by size:
2037 1647 455
BsaHI Gr'CG_yC
Cuts at: 543 1570 1818 2520 4016 543
Size: 1027 248 702 1496 666
Fragments arranged by size:
1496 1027 702 666 248
BsaWI w'CCGG_w
Cuts at: 1328 1849 2527 2801 2948 3359 1328
Size: 521 678 274 147 411 2108
Fragments arranged by size:
2108 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 412 442 910 940 412
Size: 30 468 30 3611
Fragments arranged by size:
3611 468 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 600 603 606 624 627 862 865 600
Size: 3 3 18 3 235 3 3874
Fragments arranged by size:
3874 235 18 3 3 3 3
BseYI C'CCAG_C
Cuts at: 329 511 523 531 1564 2322 2899 3395 329
Size: 182 12 8 1033 758 577 496 1073
Fragments arranged by size:
1073 1033 758 577 496 182 12 8
BsiEI CG_ry'CG
Cuts at: 104 248 296 417 1681 1727 2935 104
Size: 144 48 121 1264 46 1208 1308
Fragments arranged by size:
1308 1264 1208 144 121 48 46
BsiHKAI G_wGCw'C
Cuts at: 658 1153 1931 2121 2913 658
Size: 495 778 190 792 1884
Fragments arranged by size:
1884 792 778 495 190
BsiWI C'GTAC_G
Cuts at: 371 371
Size: 4139
BsmI GAATG_Cn'
Cuts at: 861 861
Size: 4139
BsmBI CGTCTCn'nnnn_
Cuts at: 1124 1124
Size: 4139
Bsp1286I G_dGCh'C
Cuts at: 326 413 658 803 1153 1764 1857 1931 2121
Size: 87 245 145 350 611 93 74 190
Cuts at: 2121 2183 2913 326
Size: 62 730 1552
Fragments arranged by size:
1552 730 611 350 245 190 145 93
87 74 62
BspEI T'CCGG_A
Cuts at: 3359 3359
Size: 4139
BspHI T'CATG_A
Cuts at: 3315 3315
Size: 4139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 217 1189 1403 1705 2086 217
Size: 972 214 302 381 2270
Fragments arranged by size:
2270 972 381 302 214
BsrBI CCG'CTC
Cuts at: 50 356 574 2431 2485 3969 4134 50
Size: 306 218 1857 54 1484 165 55
Fragments arranged by size:
1857 1484 306 218 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2051 2051
Size: 4139
BsrFI r'CCGG_y
Cuts at: 218 1016 2137 2318 2527 218
Size: 798 1121 181 209 1830
Fragments arranged by size:
1830 1121 798 209 181
BssHII G'CGCG_C
Cuts at: 184 209 184
Size: 25 4114
BssSI C'ACGA_G
Cuts at: 270 309 699 2410 2768 270
Size: 39 390 1711 358 1641
Fragments arranged by size:
1711 1641 390 358 39
BstAPI GCAn_nnn'nTGC
Cuts at: 1516 1516
Size: 4139
BstBI TT'CG_AA
Cuts at: 2500 2500
Size: 4139
BstYI r'GATC_y
Cuts at: 1 914 1164 1631 1654 1989 2235 3247 3333
Size: 913 250 467 23 335 246 1012 86
Cuts at: 3333 3345 3691 1
Size: 12 346 449
Fragments arranged by size:
1012 913 467 449 346 335 250 246
86 23 12
BtgI C'CryG_G
Cuts at: 317 504 590 659 317
Size: 187 86 69 3797
Fragments arranged by size:
3797 187 86 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 364 2089 2270 2433 364
Size: 1725 181 163 2070
Fragments arranged by size:
2070 1725 181 163
BtsI GCAGTG_nn'
Cuts at: 1382 1382
Size: 4139
DraI TTT'AAA
Cuts at: 1138 1138
Size: 4139
DrdI GACnn_nn'nnGTC
Cuts at: 1845 2703 1845
Size: 858 3281
EaeI y'GGCC_r
Cuts at: 245 293 392 414 1031 1724 1898 2289 2316
Size: 48 99 22 617 693 174 391 27
Cuts at: 2316 3661 245
Size: 1345 723
Fragments arranged by size:
1345 723 693 617 391 174 99 48
27 22
EagI C'GGCC_G
Cuts at: 245 293 414 1724 245
Size: 48 121 1310 2660
Fragments arranged by size:
2660 1310 121 48
EarI CTCTTCn'nnn_
Cuts at: 997 2162 2372 997
Size: 1165 210 2764
Fragments arranged by size:
2764 1165 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2347 2657 2803 3949 2347
Size: 310 146 1146 2537
Fragments arranged by size:
2537 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1151 1151
Size: 4139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 862 997 1964 2396 3143 3411 862
Size: 135 967 432 747 268 1590
Fragments arranged by size:
1590 967 747 432 268 135
EcoNI CCTnn'n_nnAGG
Cuts at: 758 758
Size: 4139
EcoO109I rG'GnC_Cy
Cuts at: 1278 1278
Size: 4139
EcoRI G'AATT_C
Cuts at: 985 1143 985
Size: 158 3981
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 4139
FseI GG_CCGG'CC
Cuts at: 222 222
Size: 4139
FspI TGC'GCA
Cuts at: 1920 1920
Size: 4139
HaeII r_GCGC'y
Cuts at: 546 945 1023 1573 1821 2843 3822 546
Size: 399 78 550 248 1022 979 863
Fragments arranged by size:
1022 979 863 550 399 248 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 906 938 1981 2011 2013 2043 906
Size: 32 1043 30 2 30 3002
Fragments arranged by size:
3002 1043 32 30 30 2
HincII GTy'rAC
Cuts at: 1178 1178
Size: 4139
KasI G'GCGC_C
Cuts at: 542 1569 1817 542
Size: 1027 248 2864
Fragments arranged by size:
2864 1027 248
KpnI G_GTAC'C
Cuts at: 1159 1159
Size: 4139
MluI A'CGCG_T
Cuts at: 3572 3572
Size: 4139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 776 2810 2994 776
Size: 2034 184 1921
Fragments arranged by size:
2034 1921 184
MscI TGG'CCA
Cuts at: 1033 1900 1033
Size: 867 3272
MslI CAynn'nnrTG
Cuts at: 2128 2255 2128
Size: 127 4012
MspA1I CmG'CkG
Cuts at: 319 343 444 506 527 686 733 1250 1564
Size: 24 101 62 21 159 47 517 314
Cuts at: 1564 1924 2937 3182 319
Size: 360 1013 245 1276
Fragments arranged by size:
1276 1013 517 360 314 245 159 101
62 47 24 21
NaeI GCC'GGC
Cuts at: 220 2320 220
Size: 2100 2039
NarI GG'CG_CC
Cuts at: 543 1570 1818 543
Size: 1027 248 2864
Fragments arranged by size:
2864 1027 248
NgoMIV G'CCGG_C
Cuts at: 218 2318 218
Size: 2100 2039
NspI r_CATG'y
Cuts at: 1002 1192 1692 2599 1002
Size: 190 500 907 2542
Fragments arranged by size:
2542 907 500 190
PasI CC'CwG_GG
Cuts at: 675 675
Size: 4139
PciI A'CATG_T
Cuts at: 998 2595 998
Size: 1597 2542
PfoI T'CCnGG_A
Cuts at: 430 430
Size: 4139
PmeI GTTT'AAAC
Cuts at: 1138 1138
Size: 4139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3278 3310 3278
Size: 32 4107
PstI C_TGCA'G
Cuts at: 228 719 1186 1871 228
Size: 491 467 685 2496
Fragments arranged by size:
2496 685 491 467
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4139
PvuII CAG'CTG
Cuts at: 1564 1924 1564
Size: 360 3779
RsrII CG'GwC_CG
Cuts at: 496 2334 496
Size: 1838 2301
SacI G_AGCT'C
Cuts at: 1153 1153
Size: 4139
SacII CC_GC'GG
Cuts at: 320 507 320
Size: 187 3952
SalI G'TCGA_C
Cuts at: 1176 1176
Size: 4139
SapI GCTCTTCn'nnn_
Cuts at: 2162 2372 2162
Size: 210 3929
SbfI CC_TGCA'GG
Cuts at: 228 719 1186 228
Size: 491 467 3181
Fragments arranged by size:
3181 491 467
ScaI AGT'ACT
Cuts at: 3656 3656
Size: 4139
SfcI C'TryA_G
Cuts at: 33 224 715 1182 1867 2860 3051 33
Size: 191 491 467 685 993 191 1121
Fragments arranged by size:
1121 993 685 491 467 191 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 201 527 201
Size: 326 3813
SfoI GGC'GCC
Cuts at: 544 1571 1819 544
Size: 1027 248 2864
Fragments arranged by size:
2864 1027 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
SmaI CCC'GGG
Cuts at: 458 667 1012 1161 458
Size: 209 345 149 3436
Fragments arranged by size:
3436 345 209 149
SmlI C'TyrA_G
Cuts at: 970 1294 2209 2701 2963 970
Size: 324 915 492 262 2146
Fragments arranged by size:
2146 915 492 324 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4139
SphI G_CATG'C
Cuts at: 1192 1692 1192
Size: 500 3639
StyI C'CwwG_G
Cuts at: 1273 1627 1273
Size: 354 3785
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1722 1855 2314 2523 1722
Size: 133 459 209 3338
Fragments arranged by size:
3338 459 209 133
TatI w'GTAC_w
Cuts at: 3654 3654
Size: 4139
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 910 942 3656 3688 910
Size: 32 2714 32 1361
Fragments arranged by size:
2714 1361 32 32
Tth111I GACn'n_nGTC
Cuts at: 1936 1936
Size: 4139
XbaI T'CTAG_A
Cuts at: 1170 1170
Size: 4139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1634 1634
Size: 4139
XmaI C'CCGG_G
Cuts at: 456 665 1010 1159 456
Size: 209 345 149 3436
Fragments arranged by size:
3436 345 209 149
XmnI GAAnn'nnTTC
Cuts at: 985 985
Size: 4139
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsgI BsrGI
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SanDI SexAI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI