Restriction map for
pF1KB8960
(Circular) (Six-base) MAPSORT of: pF1KB8960.seq Check: 64 from: 1 to: 4133
pF1KB8960 4133 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 341 1171 341
Size: 830 3303
Acc65I G'GTAC_C
Cuts at: 1149 1149
Size: 4133
AclI AA'CG_TT
Cuts at: 3973 3973
Size: 4133
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1958 2390 3137 3405 1958
Size: 432 747 268 2686
Fragments arranged by size:
2686 747 432 268
AflIII A'CryG_T
Cuts at: 1485 2589 3566 1485
Size: 1104 977 2052
Fragments arranged by size:
2052 1104 977
AgeI A'CCGG_T
Cuts at: 2521 2521
Size: 4133
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 229 229
Size: 4133
AlwNI CAG_nnn'CTG
Cuts at: 170 507 1438 3005 170
Size: 337 931 1567 1298
Fragments arranged by size:
1567 1298 931 337
ApaLI G'TGCA_C
Cuts at: 2903 2903
Size: 4133
ApoI r'AATT_y
Cuts at: 1137 3486 3742 1137
Size: 2349 256 1528
Fragments arranged by size:
2349 1528 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4133
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4133
AvaI C'yCGr_G
Cuts at: 763 1153 763
Size: 390 3743
BamHI G'GATC_C
Cuts at: 1094 1158 1094
Size: 64 4069
BanI G'GyrC_C
Cuts at: 917 1149 1563 1811 1846 917
Size: 232 414 248 35 3204
Fragments arranged by size:
3204 414 248 232 35
BanII G_rGCy'C
Cuts at: 719 737 763 1147 2177 719
Size: 18 26 384 1030 2675
Fragments arranged by size:
2675 1030 384 26 18
BbeI G_GCGC'C
Cuts at: 1567 1815 1567
Size: 248 3885
BbvCI CC'TCA_GC
Cuts at: 806 806
Size: 4133
BciVI GTATCCnnnnn_n'
Cuts at: 2031 2792 4133 2031
Size: 761 1341 2031
Fragments arranged by size:
2031 1341 761
BclI T'GATC_A
Cuts at: 161 1653 161
Size: 1492 2641
BglI GCCn_nnn'nGGC
Cuts at: 620 620
Size: 4133
BglII A'GATC_T
Cuts at: 364 1648 3685 364
Size: 1284 2037 812
Fragments arranged by size:
2037 1284 812
BlpI GC'TnA_GC
Cuts at: 1245 1245
Size: 4133
Bme1580I G_kGCm'C
Cuts at: 922 1758 1851 2907 922
Size: 836 93 1056 2148
Fragments arranged by size:
2148 1056 836 93
BmrI ACTGGGnnnn_n'
Cuts at: 1345 1426 1761 3905 1345
Size: 81 335 2144 1573
Fragments arranged by size:
2144 1573 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2500 2500
Size: 4133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 625
Size: 4133
Bpu10I CC'TnA_GC
Cuts at: 806 806
Size: 4133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 1054 1309 2188 2680 2978 320
Size: 734 255 879 492 298 1475
Fragments arranged by size:
1475 879 734 492 298 255
BsaI GGTCTCn'nnnn_
Cuts at: 816 3837 816
Size: 3021 1112
BsaAI yAC'GTr
Cuts at: 1486 1486
Size: 4133
BsaBI GATnn'nnATC
Cuts at: 6 1647 3684 6
Size: 1641 2037 455
Fragments arranged by size:
2037 1641 455
BsaHI Gr'CG_yC
Cuts at: 1564 1812 2514 4010 1564
Size: 248 702 1496 1687
Fragments arranged by size:
1687 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1091 1322 1843 2521 2795 2942 3353 1091
Size: 231 521 678 274 147 411 1871
Fragments arranged by size:
1871 678 521 411 274 231 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 168 198 168
Size: 30 4103
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 807 807
Size: 4133
BseYI C'CCAG_C
Cuts at: 686 1558 2316 2893 3389 686
Size: 872 758 577 496 1430
Fragments arranged by size:
1430 872 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 853 853
Size: 4133
BsiEI CG_ry'CG
Cuts at: 104 485 1675 1721 2929 104
Size: 381 1190 46 1208 1308
Fragments arranged by size:
1308 1208 1190 381 46
BsiHKAI G_wGCw'C
Cuts at: 1147 1925 2115 2907 1147
Size: 778 190 792 2373
Fragments arranged by size:
2373 792 778 190
BsmI GAATG_Cn'
Cuts at: 254 254
Size: 4133
BsmBI CGTCTCn'nnnn_
Cuts at: 278 278
Size: 4133
Bsp1286I G_dGCh'C
Cuts at: 719 737 763 922 1147 1758 1851 1925 2115
Size: 18 26 159 225 611 93 74 190
Cuts at: 2115 2177 2907 719
Size: 62 730 1945
Fragments arranged by size:
1945 730 611 225 190 159 93 74
62 26 18
BspEI T'CCGG_A
Cuts at: 3353 3353
Size: 4133
BspHI T'CATG_A
Cuts at: 3309 3309
Size: 4133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1023 1183 1397 1699 2080 1023
Size: 160 214 302 381 3076
Fragments arranged by size:
3076 381 302 214 160
BsrBI CCG'CTC
Cuts at: 50 725 2425 2479 3963 4128 50
Size: 675 1700 54 1484 165 55
Fragments arranged by size:
1700 1484 675 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2045 2045
Size: 4133
BsrFI r'CCGG_y
Cuts at: 2131 2312 2521 2131
Size: 181 209 3743
Fragments arranged by size:
3743 209 181
BsrGI T'GTAC_A
Cuts at: 209 209
Size: 4133
BssSI C'ACGA_G
Cuts at: 653 2404 2762 653
Size: 1751 358 2024
Fragments arranged by size:
2024 1751 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1510 1510
Size: 4133
BstBI TT'CG_AA
Cuts at: 2494 2494
Size: 4133
BstYI r'GATC_y
Cuts at: 1 354 364 1094 1158 1625 1648 1983 2229
Size: 353 10 730 64 467 23 335 246
Cuts at: 2229 3241 3327 3339 3685 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 730 467 449 353 346 335 246
86 64 23 12 10
Bsu36I CC'TnA_GG
Cuts at: 539 539
Size: 4133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2083 2264 2427 2083
Size: 181 163 3789
Fragments arranged by size:
3789 181 163
BtsI GCAGTG_nn'
Cuts at: 890 1376 890
Size: 486 3647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 636 671 636
Size: 35 4098
DraI TTT'AAA
Cuts at: 1132 1132
Size: 4133
DrdI GACnn_nn'nnGTC
Cuts at: 1033 1839 2697 1033
Size: 806 858 2469
Fragments arranged by size:
2469 858 806
EaeI y'GGCC_r
Cuts at: 278 482 662 726 1718 1892 2283 2310 3655
Size: 204 180 64 992 174 391 27 1345
Cuts at: 3655 278
Size: 756
Fragments arranged by size:
1345 992 756 391 204 180 174 64
27
EagI C'GGCC_G
Cuts at: 482 1718 482
Size: 1236 2897
EarI CTCTTCn'nnn_
Cuts at: 782 907 2156 2366 782
Size: 125 1249 210 2549
Fragments arranged by size:
2549 1249 210 125
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2341 2651 2797 3943 2341
Size: 310 146 1146 2531
Fragments arranged by size:
2531 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1145 1145
Size: 4133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 1958 2390 3137 3405 625
Size: 1333 432 747 268 1353
Fragments arranged by size:
1353 1333 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 805 805
Size: 4133
EcoO109I rG'GnC_Cy
Cuts at: 239 489 1272 239
Size: 250 783 3100
Fragments arranged by size:
3100 783 250
EcoRI G'AATT_C
Cuts at: 1137 1137
Size: 4133
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 796 2152 796
Size: 1356 2777
FspI TGC'GCA
Cuts at: 1914 1914
Size: 4133
HaeII r_GCGC'y
Cuts at: 203 993 1567 1815 2837 3816 203
Size: 790 574 248 1022 979 520
Fragments arranged by size:
1022 979 790 574 520 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1975 2005 2007 2037 1975
Size: 30 2 30 4071
Fragments arranged by size:
4071 30 30 2
HincII GTy'rAC
Cuts at: 1017 1172 1017
Size: 155 3978
HpaI GTT'AAC
Cuts at: 1017 1017
Size: 4133
KasI G'GCGC_C
Cuts at: 1563 1811 1563
Size: 248 3885
KpnI G_GTAC'C
Cuts at: 1153 1153
Size: 4133
MfeI C'AATT_G
Cuts at: 441 882 441
Size: 441 3692
MluI A'CGCG_T
Cuts at: 3566 3566
Size: 4133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 328 2804 2988 328
Size: 2476 184 1473
Fragments arranged by size:
2476 1473 184
MscI TGG'CCA
Cuts at: 280 1894 280
Size: 1614 2519
MslI CAynn'nnrTG
Cuts at: 211 634 2122 2249 211
Size: 423 1488 127 2095
Fragments arranged by size:
2095 1488 423 127
MspA1I CmG'CkG
Cuts at: 682 690 1244 1558 1918 2931 3176 682
Size: 8 554 314 360 1013 245 1639
Fragments arranged by size:
1639 1013 554 360 314 245 8
NaeI GCC'GGC
Cuts at: 2314 2314
Size: 4133
NarI GG'CG_CC
Cuts at: 1564 1812 1564
Size: 248 3885
NgoMIV G'CCGG_C
Cuts at: 2312 2312
Size: 4133
NspI r_CATG'y
Cuts at: 210 216 453 1186 1686 2593 210
Size: 6 237 733 500 907 1750
Fragments arranged by size:
1750 907 733 500 237 6
PciI A'CATG_T
Cuts at: 2589 2589
Size: 4133
PflMI CCAn_nnn'nTGG
Cuts at: 306 507 884 942 306
Size: 201 377 58 3497
Fragments arranged by size:
3497 377 201 58
PmeI GTTT'AAAC
Cuts at: 1132 1132
Size: 4133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 949 981 3272 3304 949
Size: 32 2291 32 1778
Fragments arranged by size:
2291 1778 32 32
PpuMI rG'GwC_Cy
Cuts at: 239 489 239
Size: 250 3883
PstI C_TGCA'G
Cuts at: 175 695 1180 1865 175
Size: 520 485 685 2443
Fragments arranged by size:
2443 685 520 485
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4133
PvuII CAG'CTG
Cuts at: 690 1558 1918 690
Size: 868 360 2905
Fragments arranged by size:
2905 868 360
RsrII CG'GwC_CG
Cuts at: 2328 2328
Size: 4133
SacI G_AGCT'C
Cuts at: 1147 1147
Size: 4133
SalI G'TCGA_C
Cuts at: 1170 1170
Size: 4133
SapI GCTCTTCn'nnn_
Cuts at: 2156 2366 2156
Size: 210 3923
SbfI CC_TGCA'GG
Cuts at: 175 1180 175
Size: 1005 3128
ScaI AGT'ACT
Cuts at: 3650 3650
Size: 4133
SfcI C'TryA_G
Cuts at: 33 171 691 1176 1861 2854 3045 33
Size: 138 520 485 685 993 191 1121
Fragments arranged by size:
1121 993 685 520 485 191 138
SfoI GGC'GCC
Cuts at: 1565 1813 1565
Size: 248 3885
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4133
SmaI CCC'GGG
Cuts at: 1155 1155
Size: 4133
SmlI C'TyrA_G
Cuts at: 335 1069 1288 2203 2695 2957 335
Size: 734 219 915 492 262 1511
Fragments arranged by size:
1511 915 734 492 262 219
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4133
SphI G_CATG'C
Cuts at: 1186 1686 1186
Size: 500 3633
StyI C'CwwG_G
Cuts at: 1267 1621 1267
Size: 354 3779
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1716 1849 2308 2517 1716
Size: 133 459 209 3332
Fragments arranged by size:
3332 459 209 133
TatI w'GTAC_w
Cuts at: 209 602 3648 209
Size: 393 3046 694
Fragments arranged by size:
3046 694 393
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 383 415 3650 3682 383
Size: 32 3235 32 834
Fragments arranged by size:
3235 834 32 32
Tth111I GACn'n_nGTC
Cuts at: 569 1930 569
Size: 1361 2772
XbaI T'CTAG_A
Cuts at: 1164 1164
Size: 4133
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 881 1628 881
Size: 747 3386
XmaI C'CCGG_G
Cuts at: 1153 1153
Size: 4133
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI
BsiWI BssHII BstEII BstXI BstZ17I BtgI ClaI DraIII
EcoRV FseI FspAI HindIII NcoI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI