Restriction map for
pF1KB8959
(Circular) (Six-base) MAPSORT of: pF1KB8959.seq Check: 4594 from: 1 to: 4127
pF1KB8959 4127 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 578 578
Size: 4127
AccI GT'mk_AC
Cuts at: 1165 1165
Size: 4127
Acc65I G'GTAC_C
Cuts at: 1143 1143
Size: 4127
AclI AA'CG_TT
Cuts at: 3967 3967
Size: 4127
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1952 2384 3131 3399 1952
Size: 432 747 268 2680
Fragments arranged by size:
2680 747 432 268
AfeI AGC'GCT
Cuts at: 685 685
Size: 4127
AflIII A'CryG_T
Cuts at: 1479 2583 3560 1479
Size: 1104 977 2046
Fragments arranged by size:
2046 1104 977
AgeI A'CCGG_T
Cuts at: 2515 2515
Size: 4127
AhdI GACnn_n'nnGTC
Cuts at: 648 648
Size: 4127
AlwNI CAG_nnn'CTG
Cuts at: 1432 2999 1432
Size: 1567 2560
ApaLI G'TGCA_C
Cuts at: 439 2897 439
Size: 2458 1669
ApoI r'AATT_y
Cuts at: 166 1131 3480 3736 166
Size: 965 2349 256 557
Fragments arranged by size:
2349 965 557 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4127
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4127
AvaI C'yCGr_G
Cuts at: 973 1147 973
Size: 174 3953
BamHI G'GATC_C
Cuts at: 1152 1152
Size: 4127
BanI G'GyrC_C
Cuts at: 556 954 1143 1557 1805 1840 556
Size: 398 189 414 248 35 2843
Fragments arranged by size:
2843 414 398 248 189 35
BanII G_rGCy'C
Cuts at: 772 911 1141 2171 772
Size: 139 230 1030 2728
Fragments arranged by size:
2728 1030 230 139
BbeI G_GCGC'C
Cuts at: 560 958 1561 1809 560
Size: 398 603 248 2878
Fragments arranged by size:
2878 603 398 248
BbvCI CC'TCA_GC
Cuts at: 637 637
Size: 4127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 930 964 930
Size: 34 4093
BciVI GTATCCnnnnn_n'
Cuts at: 2025 2786 4127 2025
Size: 761 1341 2025
Fragments arranged by size:
2025 1341 761
BclI T'GATC_A
Cuts at: 1647 1647
Size: 4127
BglI GCCn_nnn'nGGC
Cuts at: 112 455 112
Size: 343 3784
BglII A'GATC_T
Cuts at: 1642 3679 1642
Size: 2037 2090
BlpI GC'TnA_GC
Cuts at: 775 1239 775
Size: 464 3663
Bme1580I G_kGCm'C
Cuts at: 368 395 443 1752 1845 2901 368
Size: 27 48 1309 93 1056 1594
Fragments arranged by size:
1594 1309 1056 93 48 27
BmgBI CAC'GTC
Cuts at: 374 645 729 944 1000 374
Size: 271 84 215 56 3501
Fragments arranged by size:
3501 271 215 84 56
BmrI ACTGGGnnnn_n'
Cuts at: 1339 1420 1755 3899 1339
Size: 81 335 2144 1567
Fragments arranged by size:
2144 1567 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 229 492 2494 229
Size: 263 2002 1862
Fragments arranged by size:
2002 1862 263
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 487 835 156
Size: 331 348 3448
Fragments arranged by size:
3448 348 331
Bpu10I CC'TnA_GC
Cuts at: 637 637
Size: 4127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 823 1303 2182 2674 2972 736
Size: 87 480 879 492 298 1891
Fragments arranged by size:
1891 879 492 480 298 87
BsaI GGTCTCn'nnnn_
Cuts at: 3831 3831
Size: 4127
BsaAI yAC'GTr
Cuts at: 1480 1480
Size: 4127
BsaBI GATnn'nnATC
Cuts at: 6 1641 3678 6
Size: 1635 2037 455
Fragments arranged by size:
2037 1635 455
BsaHI Gr'CG_yC
Cuts at: 338 557 955 1558 1806 2508 4004 338
Size: 219 398 603 248 702 1496 461
Fragments arranged by size:
1496 702 603 461 398 248 219
BsaWI w'CCGG_w
Cuts at: 510 1316 1837 2515 2789 2936 3347 510
Size: 806 521 678 274 147 411 1290
Fragments arranged by size:
1290 806 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 172 202 172
Size: 30 4097
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 880 880
Size: 4127
BseYI C'CCAG_C
Cuts at: 216 405 708 1552 2310 2887 3383 216
Size: 189 303 844 758 577 496 960
Fragments arranged by size:
960 844 758 577 496 303 189
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 354 556 163
Size: 191 202 3734
Fragments arranged by size:
3734 202 191
BsiEI CG_ry'CG
Cuts at: 104 1669 1715 2923 104
Size: 1565 46 1208 1308
Fragments arranged by size:
1565 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 443 473 851 911 1141 1919 2109 2901 443
Size: 30 378 60 230 778 190 792 1669
Fragments arranged by size:
1669 792 778 378 230 190 60 30
BsmBI CGTCTCn'nnnn_
Cuts at: 309 309
Size: 4127
Bsp1286I G_dGCh'C
Cuts at: 368 395 443 473 772 851 911 1141 1752
Size: 27 48 30 299 79 60 230 611
Cuts at: 1752 1845 1919 2109 2171 2901 368
Size: 93 74 190 62 730 1594
Fragments arranged by size:
1594 730 611 299 230 190 93 79
74 62 60 48 30 27
BspEI T'CCGG_A
Cuts at: 510 3347 510
Size: 2837 1290
BspHI T'CATG_A
Cuts at: 3303 3303
Size: 4127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 470 578 785 1177 1391 1693 2074 470
Size: 108 207 392 214 302 381 2523
Fragments arranged by size:
2523 392 381 302 214 207 108
BsrBI CCG'CTC
Cuts at: 50 497 2419 2473 3957 4122 50
Size: 447 1922 54 1484 165 55
Fragments arranged by size:
1922 1484 447 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2039 2039
Size: 4127
BsrFI r'CCGG_y
Cuts at: 843 2125 2306 2515 843
Size: 1282 181 209 2455
Fragments arranged by size:
2455 1282 209 181
BssSI C'ACGA_G
Cuts at: 2398 2756 2398
Size: 358 3769
BstAPI GCAn_nnn'nTGC
Cuts at: 150 791 1504 150
Size: 641 713 2773
Fragments arranged by size:
2773 713 641
BstBI TT'CG_AA
Cuts at: 2488 2488
Size: 4127
BstEII G'GTnAC_C
Cuts at: 714 714
Size: 4127
BstYI r'GATC_y
Cuts at: 1 536 755 1152 1619 1642 1977 2223 3235
Size: 535 219 397 467 23 335 246 1012
Cuts at: 3235 3321 3333 3679 1
Size: 86 12 346 449
Fragments arranged by size:
1012 535 467 449 397 346 335 246
219 86 23 12
BtgI C'CryG_G
Cuts at: 309 560 581 605 309
Size: 251 21 24 3831
Fragments arranged by size:
3831 251 24 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 933 1011 2077 2258 2421 933
Size: 78 1066 181 163 2639
Fragments arranged by size:
2639 1066 181 163 78
BtsI GCAGTG_nn'
Cuts at: 1370 1370
Size: 4127
DraI TTT'AAA
Cuts at: 1126 1126
Size: 4127
DraIII CAC_nnn'GTG
Cuts at: 150 438 522 606 150
Size: 288 84 84 3671
Fragments arranged by size:
3671 288 84 84
DrdI GACnn_nn'nnGTC
Cuts at: 1833 2691 1833
Size: 858 3269
EaeI y'GGCC_r
Cuts at: 113 1022 1712 1886 2277 2304 3649 113
Size: 909 690 174 391 27 1345 591
Fragments arranged by size:
1345 909 690 591 391 174 27
EagI C'GGCC_G
Cuts at: 1712 1712
Size: 4127
EarI CTCTTCn'nnn_
Cuts at: 916 2150 2360 916
Size: 1234 210 2683
Fragments arranged by size:
2683 1234 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 739 2335 2645 2791 3937 739
Size: 1596 310 146 1146 929
Fragments arranged by size:
1596 1146 929 310 146
EcoICRI GAG'CTC
Cuts at: 909 1139 909
Size: 230 3897
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 487 835 1952 2384 3131 3399 156
Size: 331 348 1117 432 747 268 884
Fragments arranged by size:
1117 884 747 432 348 331 268
EcoNI CCTnn'n_nnAGG
Cuts at: 574 574
Size: 4127
EcoO109I rG'GnC_Cy
Cuts at: 447 1266 447
Size: 819 3308
EcoRI G'AATT_C
Cuts at: 1131 1131
Size: 4127
EcoRV GAT'ATC
Cuts at: 300 300
Size: 4127
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2146 2146
Size: 4127
FspI TGC'GCA
Cuts at: 1908 1908
Size: 4127
HaeII r_GCGC'y
Cuts at: 509 560 623 687 958 1561 1809 2831 3810
Size: 51 63 64 271 603 248 1022 979
Cuts at: 3810 509
Size: 826
Fragments arranged by size:
1022 979 826 603 271 248 64 63
51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 289 321 634 666 1969 1999 2001 2031 289
Size: 32 313 32 1303 30 2 30 2385
Fragments arranged by size:
2385 1303 313 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1166 1166
Size: 4127
HindIII A'AGCT_T
Cuts at: 249 249
Size: 4127
KasI G'GCGC_C
Cuts at: 556 954 1557 1805 556
Size: 398 603 248 2878
Fragments arranged by size:
2878 603 398 248
KpnI G_GTAC'C
Cuts at: 1147 1147
Size: 4127
MluI A'CGCG_T
Cuts at: 3560 3560
Size: 4127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 842 2798 2982 842
Size: 1956 184 1987
Fragments arranged by size:
1987 1956 184
MscI TGG'CCA
Cuts at: 115 1888 115
Size: 1773 2354
MslI CAynn'nnrTG
Cuts at: 586 2116 2243 586
Size: 1530 127 2470
Fragments arranged by size:
2470 1530 127
MspA1I CmG'CkG
Cuts at: 228 409 696 1238 1552 1912 2925 3170 228
Size: 181 287 542 314 360 1013 245 1185
Fragments arranged by size:
1185 1013 542 360 314 287 245 181
NaeI GCC'GGC
Cuts at: 2308 2308
Size: 4127
NarI GG'CG_CC
Cuts at: 557 955 1558 1806 557
Size: 398 603 248 2878
Fragments arranged by size:
2878 603 398 248
NcoI C'CATG_G
Cuts at: 560 581 560
Size: 21 4106
NgoMIV G'CCGG_C
Cuts at: 2306 2306
Size: 4127
NspI r_CATG'y
Cuts at: 144 635 1180 1680 2587 144
Size: 491 545 500 907 1684
Fragments arranged by size:
1684 907 545 500 491
PciI A'CATG_T
Cuts at: 2583 2583
Size: 4127
PflMI CCAn_nnn'nTGG
Cuts at: 582 582
Size: 4127
PmeI GTTT'AAAC
Cuts at: 1126 1126
Size: 4127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3266 3298 3266
Size: 32 4095
PspXI vC'TCGA_Gb
Cuts at: 973 973
Size: 4127
PstI C_TGCA'G
Cuts at: 233 796 1174 1859 233
Size: 563 378 685 2501
Fragments arranged by size:
2501 685 563 378
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4127
PvuII CAG'CTG
Cuts at: 228 409 1552 1912 228
Size: 181 1143 360 2443
Fragments arranged by size:
2443 1143 360 181
RsrII CG'GwC_CG
Cuts at: 2322 2322
Size: 4127
SacI G_AGCT'C
Cuts at: 911 1141 911
Size: 230 3897
SalI G'TCGA_C
Cuts at: 1164 1164
Size: 4127
SapI GCTCTTCn'nnn_
Cuts at: 916 2150 2360 916
Size: 1234 210 2683
Fragments arranged by size:
2683 1234 210
SbfI CC_TGCA'GG
Cuts at: 1174 1174
Size: 4127
ScaI AGT'ACT
Cuts at: 3644 3644
Size: 4127
SfcI C'TryA_G
Cuts at: 33 229 792 1170 1855 2848 3039 33
Size: 196 563 378 685 993 191 1121
Fragments arranged by size:
1121 993 685 563 378 196 191
SfoI GGC'GCC
Cuts at: 558 956 1559 1807 558
Size: 398 603 248 2878
Fragments arranged by size:
2878 603 398 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4127
SmaI CCC'GGG
Cuts at: 1149 1149
Size: 4127
SmlI C'TyrA_G
Cuts at: 751 838 973 1282 2197 2689 2951 751
Size: 87 135 309 915 492 262 1927
Fragments arranged by size:
1927 915 492 309 262 135 87
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4127
SphI G_CATG'C
Cuts at: 144 635 1180 1680 144
Size: 491 545 500 2591
Fragments arranged by size:
2591 545 500 491
StuI AGG'CCT
Cuts at: 856 979 856
Size: 123 4004
StyI C'CwwG_G
Cuts at: 560 581 1261 1615 560
Size: 21 680 354 3072
Fragments arranged by size:
3072 680 354 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 419 1710 1843 2302 2511 419
Size: 1291 133 459 209 2035
Fragments arranged by size:
2035 1291 459 209 133
TatI w'GTAC_w
Cuts at: 3642 3642
Size: 4127
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 172 204 3644 3676 172
Size: 32 3440 32 623
Fragments arranged by size:
3440 623 32 32
Tth111I GACn'n_nGTC
Cuts at: 1924 1924
Size: 4127
XbaI T'CTAG_A
Cuts at: 1158 1158
Size: 4127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1622 1622
Size: 4127
XhoI C'TCGA_G
Cuts at: 973 973
Size: 4127
XmaI C'CCGG_G
Cuts at: 1147 1147
Size: 4127
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AflII AleI AlfI AloI ApaI AscI AvrII
BaeI BbsI BfrBI BmtI BsiWI BsmI BsrGI BssHII
BstXI BstZ17I Bsu36I ClaI CspCI FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XmnI ZraI