Restriction map for
pF1KB8958
(Circular) (Six-base) MAPSORT of: pF1KB8958.seq Check: 3229 from: 1 to: 4118
pF1KB8958 4118 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1156 1156
Size: 4118
Acc65I G'GTAC_C
Cuts at: 473 1134 473
Size: 661 3457
AclI AA'CG_TT
Cuts at: 3958 3958
Size: 4118
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 402 1943 2375 3122 3390 402
Size: 1541 432 747 268 1130
Fragments arranged by size:
1541 1130 747 432 268
AflIII A'CryG_T
Cuts at: 440 1470 2574 3551 440
Size: 1030 1104 977 1007
Fragments arranged by size:
1104 1030 1007 977
AgeI A'CCGG_T
Cuts at: 2506 2506
Size: 4118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 298 298
Size: 4118
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 832 864 832
Size: 32 4086
AlwNI CAG_nnn'CTG
Cuts at: 618 1423 2990 618
Size: 805 1567 1746
Fragments arranged by size:
1746 1567 805
ApaLI G'TGCA_C
Cuts at: 2888 2888
Size: 4118
ApoI r'AATT_y
Cuts at: 1122 3471 3727 1122
Size: 2349 256 1513
Fragments arranged by size:
2349 1513 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4118
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
AvaI C'yCGr_G
Cuts at: 165 182 1074 1138 165
Size: 17 892 64 3145
Fragments arranged by size:
3145 892 64 17
BamHI G'GATC_C
Cuts at: 1143 1143
Size: 4118
BanI G'GyrC_C
Cuts at: 473 1134 1548 1796 1831 473
Size: 661 414 248 35 2760
Fragments arranged by size:
2760 661 414 248 35
BanII G_rGCy'C
Cuts at: 574 1132 2162 574
Size: 558 1030 2530
Fragments arranged by size:
2530 1030 558
BbeI G_GCGC'C
Cuts at: 1552 1800 1552
Size: 248 3870
BbsI GAAGACnn'nnnn_
Cuts at: 1073 1073
Size: 4118
BciVI GTATCCnnnnn_n'
Cuts at: 2016 2777 4118 2016
Size: 761 1341 2016
Fragments arranged by size:
2016 1341 761
BclI T'GATC_A
Cuts at: 1638 1638
Size: 4118
BglII A'GATC_T
Cuts at: 929 1633 3670 929
Size: 704 2037 1377
Fragments arranged by size:
2037 1377 704
BlpI GC'TnA_GC
Cuts at: 177 1230 177
Size: 1053 3065
Bme1580I G_kGCm'C
Cuts at: 1743 1836 2892 1743
Size: 93 1056 2969
Fragments arranged by size:
2969 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 474 1330 1411 1746 3890 474
Size: 856 81 335 2144 702
Fragments arranged by size:
2144 856 702 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1053 2485 1053
Size: 1432 2686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 692 859 692
Size: 167 3951
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 369 1294 2173 2665 2963 369
Size: 925 879 492 298 1524
Fragments arranged by size:
1524 925 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 910 985 3822 910
Size: 75 2837 1206
Fragments arranged by size:
2837 1206 75
BsaAI yAC'GTr
Cuts at: 632 1471 632
Size: 839 3279
BsaBI GATnn'nnATC
Cuts at: 6 1632 3669 6
Size: 1626 2037 455
Fragments arranged by size:
2037 1626 455
BsaHI Gr'CG_yC
Cuts at: 1549 1797 2499 3995 1549
Size: 248 702 1496 1672
Fragments arranged by size:
1672 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1307 1828 2506 2780 2927 3338 1307
Size: 521 678 274 147 411 2087
Fragments arranged by size:
2087 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 690 720 832 862 690
Size: 30 112 30 3946
Fragments arranged by size:
3946 112 30 30
BseYI C'CCAG_C
Cuts at: 332 556 1543 2301 2878 3374 332
Size: 224 987 758 577 496 1076
Fragments arranged by size:
1076 987 758 577 496 224
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 304
Size: 4118
BsiEI CG_ry'CG
Cuts at: 104 261 1660 1706 2914 104
Size: 157 1399 46 1208 1308
Fragments arranged by size:
1399 1308 1208 157 46
BsiHKAI G_wGCw'C
Cuts at: 611 1132 1910 2100 2892 611
Size: 521 778 190 792 1837
Fragments arranged by size:
1837 792 778 521 190
Bsp1286I G_dGCh'C
Cuts at: 574 611 1132 1743 1836 1910 2100 2162 2892
Size: 37 521 611 93 74 190 62 730
Cuts at: 2892 574
Size: 1800
Fragments arranged by size:
1800 730 611 521 190 93 74 62
37
BspEI T'CCGG_A
Cuts at: 3338 3338
Size: 4118
BspHI T'CATG_A
Cuts at: 3294 3294
Size: 4118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 181 1168 1382 1684 2065 181
Size: 987 214 302 381 2234
Fragments arranged by size:
2234 987 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2410 2464 3948 4113 50
Size: 2360 54 1484 165 55
Fragments arranged by size:
2360 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2030 2030
Size: 4118
BsrFI r'CCGG_y
Cuts at: 336 2116 2297 2506 336
Size: 1780 181 209 1948
Fragments arranged by size:
1948 1780 209 181
BssHII G'CGCG_C
Cuts at: 873 873
Size: 4118
BssSI C'ACGA_G
Cuts at: 2389 2747 2389
Size: 358 3760
BstAPI GCAn_nnn'nTGC
Cuts at: 1495 1495
Size: 4118
BstBI TT'CG_AA
Cuts at: 2479 2479
Size: 4118
BstXI CCAn_nnnn'nTGG
Cuts at: 598 598
Size: 4118
BstYI r'GATC_y
Cuts at: 1 929 1143 1610 1633 1968 2214 3226 3312
Size: 928 214 467 23 335 246 1012 86
Cuts at: 3312 3324 3670 1
Size: 12 346 449
Fragments arranged by size:
1012 928 467 449 346 335 246 214
86 23 12
BtgI C'CryG_G
Cuts at: 107 521 107
Size: 414 3704
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2068 2249 2412 2068
Size: 181 163 3774
Fragments arranged by size:
3774 181 163
BtsI GCAGTG_nn'
Cuts at: 162 1361 162
Size: 1199 2919
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 370 405 370
Size: 35 4083
DraI TTT'AAA
Cuts at: 1117 1117
Size: 4118
DrdI GACnn_nn'nnGTC
Cuts at: 1824 2682 1824
Size: 858 3260
EaeI y'GGCC_r
Cuts at: 998 1703 1877 2268 2295 3640 998
Size: 705 174 391 27 1345 1476
Fragments arranged by size:
1476 1345 705 391 174 27
EagI C'GGCC_G
Cuts at: 1703 1703
Size: 4118
EarI CTCTTCn'nnn_
Cuts at: 1059 2141 2351 1059
Size: 1082 210 2826
Fragments arranged by size:
2826 1082 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2326 2636 2782 3928 2326
Size: 310 146 1146 2516
Fragments arranged by size:
2516 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1130 1130
Size: 4118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 402 692 859 1943 2375 3122 3390 402
Size: 290 167 1084 432 747 268 1130
Fragments arranged by size:
1130 1084 747 432 290 268 167
EcoO109I rG'GnC_Cy
Cuts at: 170 1257 170
Size: 1087 3031
EcoRI G'AATT_C
Cuts at: 1122 1122
Size: 4118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 4118
FspI TGC'GCA
Cuts at: 805 1899 805
Size: 1094 3024
FspAI rTGC'GCAy
Cuts at: 805 805
Size: 4118
HaeII r_GCGC'y
Cuts at: 239 1552 1800 2822 3801 239
Size: 1313 248 1022 979 556
Fragments arranged by size:
1313 1022 979 556 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 690 722 1960 1990 1992 2022 690
Size: 32 1238 30 2 30 2786
Fragments arranged by size:
2786 1238 32 30 30 2
HincII GTy'rAC
Cuts at: 790 1157 790
Size: 367 3751
KasI G'GCGC_C
Cuts at: 1548 1796 1548
Size: 248 3870
KpnI G_GTAC'C
Cuts at: 477 1138 477
Size: 661 3457
MluI A'CGCG_T
Cuts at: 440 3551 440
Size: 3111 1007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 747 2789 2973 747
Size: 2042 184 1892
Fragments arranged by size:
2042 1892 184
MscI TGG'CCA
Cuts at: 1879 1879
Size: 4118
MslI CAynn'nnrTG
Cuts at: 596 2107 2234 596
Size: 1511 127 2480
Fragments arranged by size:
2480 1511 127
MspA1I CmG'CkG
Cuts at: 493 1229 1543 1903 2916 3161 493
Size: 736 314 360 1013 245 1450
Fragments arranged by size:
1450 1013 736 360 314 245
NaeI GCC'GGC
Cuts at: 338 2299 338
Size: 1961 2157
NarI GG'CG_CC
Cuts at: 1549 1797 1549
Size: 248 3870
NcoI C'CATG_G
Cuts at: 107 521 107
Size: 414 3704
NdeI CA'TA_TG
Cuts at: 597 597
Size: 4118
NgoMIV G'CCGG_C
Cuts at: 336 2297 336
Size: 1961 2157
NspI r_CATG'y
Cuts at: 1171 1671 2578 1171
Size: 500 907 2711
Fragments arranged by size:
2711 907 500
PciI A'CATG_T
Cuts at: 2574 2574
Size: 4118
PflMI CCAn_nnn'nTGG
Cuts at: 403 618 403
Size: 215 3903
PfoI T'CCnGG_A
Cuts at: 134 355 134
Size: 221 3897
PmeI GTTT'AAAC
Cuts at: 1117 1117
Size: 4118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 832 864 3257 3289 832
Size: 32 2393 32 1661
Fragments arranged by size:
2393 1661 32 32
PpuMI rG'GwC_Cy
Cuts at: 170 170
Size: 4118
PstI C_TGCA'G
Cuts at: 908 1165 1850 908
Size: 257 685 3176
Fragments arranged by size:
3176 685 257
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4118
PvuII CAG'CTG
Cuts at: 1543 1903 1543
Size: 360 3758
RsrII CG'GwC_CG
Cuts at: 2313 2313
Size: 4118
SacI G_AGCT'C
Cuts at: 1132 1132
Size: 4118
SalI G'TCGA_C
Cuts at: 1155 1155
Size: 4118
SapI GCTCTTCn'nnn_
Cuts at: 1059 2141 2351 1059
Size: 1082 210 2826
Fragments arranged by size:
2826 1082 210
SbfI CC_TGCA'GG
Cuts at: 1165 1165
Size: 4118
ScaI AGT'ACT
Cuts at: 3635 3635
Size: 4118
SfcI C'TryA_G
Cuts at: 33 904 1161 1846 2839 3030 33
Size: 871 257 685 993 191 1121
Fragments arranged by size:
1121 993 871 685 257 191
SfoI GGC'GCC
Cuts at: 1550 1798 1550
Size: 248 3870
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
SmaI CCC'GGG
Cuts at: 1076 1140 1076
Size: 64 4054
SmlI C'TyrA_G
Cuts at: 384 1273 2188 2680 2942 384
Size: 889 915 492 262 1560
Fragments arranged by size:
1560 915 889 492 262
SnaBI TAC'GTA
Cuts at: 632 632
Size: 4118
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4118
SphI G_CATG'C
Cuts at: 1171 1671 1171
Size: 500 3618
StyI C'CwwG_G
Cuts at: 107 521 1252 1606 107
Size: 414 731 354 2619
Fragments arranged by size:
2619 731 414 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1701 1834 2293 2502 1701
Size: 133 459 209 3317
Fragments arranged by size:
3317 459 209 133
TatI w'GTAC_w
Cuts at: 3633 3633
Size: 4118
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3635 3667 3635
Size: 32 4086
Tth111I GACn'n_nGTC
Cuts at: 1915 1915
Size: 4118
XbaI T'CTAG_A
Cuts at: 1149 1149
Size: 4118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 388 553 1613 388
Size: 165 1060 2893
Fragments arranged by size:
2893 1060 165
XmaI C'CCGG_G
Cuts at: 1074 1138 1074
Size: 64 4054
XmnI GAAnn'nnTTC
Cuts at: 130 847 130
Size: 717 3401
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BstEII BstZ17I
Bsu36I ClaI DraIII EcoNI EcoRV FseI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SspI StuI SwaI XhoI ZraI